茉莉酸甲酯对木豆不定根染料木素含量及抗氧化系统影响
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摘要
为探讨茉莉酸甲酯(Methyl Jasmonate,MJ)对木豆不定根影响,研究了茉莉酸甲酯的不同浓度及处理时间对木豆不定根中染料木素的含量及抗氧化系统的影响。研究结果表明,诱导子的浓度和处理时间对不定根中染料木素的含量有重要影响。在MJ浓度为100μmol·L~(-1),处理时间为48 h时,染料木素的含量最高,为1.38 mg·g~(-1),是对照组的4.03倍。在此基础上,研究了100μmol·L~(-1)的MJ处理对木豆不定根抗氧化系统的影响,结果表明,MJ处理的木豆不定根明显发生了氧化胁迫,O_2~-速率、H_2O_2和MDA含量明显升高,同时不定根中SOD和POD活性明显升高,但是抗氧化酶活性的提高并未消除不定根的氧化胁迫,这种氧化胁迫导致了木豆不定根次生代谢产物合成能力提高。
We studied the effect of different concentrations and treatment time of Methyl jasmonate(MJ)ongenistein production in adventitious roots.The concentrations and treatment time of MJ had a significant effect on the content of genistein in pigeon pea adventitious roots.When the concentration of MJ was 100μmol·L~(-1),and the treatment time was 48 h,the content of genistein was the highest(1.38 mg·g~(-1)),which was 4.03 times higher than that of the control.We studied the effect of MJ(100μmol·L~(-1))on the antioxidant system inpigeon pea adventitious roots.The adventitious roots treated with MJ appeared obvious oxidative stress,the contents of O_2~-,H_2O_2 and MDA increased significantly,and the activities of SOD and POD also increased significantly.However,the increase of antioxidant enzyme activity couldnot eliminate the oxidative stress inpigeon peaadventitious roots,leding to the improvement of secondary metabolites synthesis.
We studied the effect of different concentrations and treatment time of Methyl jasmonate(MJ)ongenistein production in adventitious roots.The concentrations and treatment time of MJ had a significant effect on the content of genistein in pigeon pea adventitious roots.When the concentration of MJ was 100μmol·L~(-1),and the treatment time was 48 h,the content of genistein was the highest(1.38 mg·g~(-1)),which was 4.03 times higher than that of the control.We studied the effect of MJ(100μmol·L~(-1))on the antioxidant system inpigeon pea adventitious roots.The adventitious roots treated with MJ appeared obvious oxidative stress,the contents of O_2~-,H_2O_2 and MDA increased significantly,and the activities of SOD and POD also increased significantly.However,the increase of antioxidant enzyme activity couldnot eliminate the oxidative stress inpigeon peaadventitious roots,leding to the improvement of secondary metabolites synthesis.
引文
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17.Hashemi S M,Naghavi M R.Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L.using abiotic elicitors[J].Plant Cell,Tissue and Organ Culture(PCTOC),2016,125(1):31-41.
18.Sirikantaramas S,Yamazaki M,Saito K.Mechanisms of resistance to self-produced toxic secondary metabolites in plants[J].Phytochemistry Reviews,2008,7(3):467-477.
19.孟书亦,马秀杰,韩梅,等.水杨酸对黄芩愈伤组织抗氧化酶活性及黄芩苷含量的影响[J].分子植物育种,2017,15(10):4179-4183.Meng S Y,Ma X J,Han M,et al.Effects of salicylic acid on antioxidant enzyme activity and content of baicalin in Scutellaria baicalensis callus[J].Molecular Plant Breeding,2017,15(10):4179-4183.
20.Kim O T,Bang K H,Kim Y C,et al.Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate[J].Plant Cell,Tissue and Organ Culture(PC-TOC),2009,98(1):25-33.
21.Song S H,Byun S Y.Characterization of cell growth and camptothecin production in cell cultures of Camptotheca acuminata[J].Journal of Microbiology&Biotechnology,1998,8(6):631-638.
22.Li J,Wang J,Li J X,et al.Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.:bioactive compounds and antioxidant enzymes[J].Research on Chemical Intermediates,2016,42(2):1503-1519.
23.Li T T,Hu Y Y,Du X H,et al.Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv.merrillii seedlings by activating photosynthesis and enhancing antioxidant systems[J].PLo S One,2014,9(10):e109492.
24.赵晓菊,张丽霞,满秀玲.NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响[J].植物研究,2018,38(5):669-674.Zhao X J,Zhang L X,Man X L.Effects of exogenous NOon seed germination and physiological metabolism in Catharanthus roseus seedling under Na Cl stress.Bulletin of Botanical Research,2018,38(5):669-674.
25.Farooq M A,Gill R A,Islam F,et al.Methyl jasmonate regulates antioxidant defense and suppresses arsenic uptake in Brassica napus L.[J].Frontiers in Plant Science,2016,7:468.
26.Li J X,Wang J,Wu X L,et al.Jasmonic acid and Methyl dihydrojasmonate enhance saponin biosynthesis as well as expression of functional genes in adventitious roots of Panax notoginseng F.H.Chen[J].Biotechnology and Applied Biochemistry,2017,64(2):225-238.
2.Zhang D Y,Zu Y G,FU Y J,et al.Aqueous two-phase extraction and enrichment of two main flavonoids from pigeon pea roots and the antioxidant activity[J].Separation andPurification Technology,2013,102:26-33.
3.Pal D,Mishra P,Sachan N,et al.Biological activities and medicinal properties of Cajanus cajan(L)Millsp.[J].Journal of Advanced Pharmaceutical Technology&Research,2011,2(4):207-214.
4.Zhang D Y,Zhang S,Zu Y G,et al.Negative pressure cavitation extraction and antioxidant activity of genistein and genistin from the roots of pigeon pea(Cajanus cajan(L.)Millsp.)[J].Separation and Purification Technology,2010,74(2):261-270.
5.李琰,赵磊,崔蕾,等.诱导子对雷公藤不定根生长和次生代谢产物含量的影响[J].生物工程学报,2015,31(5):734-743.Li Y,Zhao L,Cui L,et al.Effects of elicitors on growth of adventitious roots and contents of secondary metabolites in Tripterygium wilfordii Hook.f.[J].Chinese Journal of Biotechnology,2015,31(5):734-743.
6.Qiu Z B,Guo J L,Zhu A J,et al.Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress[J].Ecotoxicology and Environmental Safety,2014,104:202-208.
7.忽雪琦,李东阳,严加坤,等.干旱胁迫下外源茉莉酸甲酯对玉米幼苗根系吸水的影响[J].植物生理学报,2018,54(6):991-998.Hu X Q,Li D Y,Yan J K,et al.Effects of exogenous methyl jasmonate on water absorption capacity of maize(Zea mays L.)seedling root under drought stress[J].Plant Physiology Journal,2018,54(6):991-998.
8.Wang C H,Zheng L P,Tian H,et al.Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots[J].Journal of Photochemistry and Photobiology B:Biology,2016,159:93-100.
9.Ahnj K,Lee W Y,Park E J.Effect of methyl jasmonate on the root growth and the eleutheroside accumulation in the adventitious root culture of Eleutherococcus senticosus[J].Journal of Korean Society of Forest Science,2010,99(3):331-336.
10.Marsh Z,Yang T H,Nopo-Olazabal L,et al.Effect of light,methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora[J].Phytochemistry,2014,107:50-60.
11.范艳敏,党士坤,王文杰,等.碳源、生长素及诱导子对木豆不定根生长和次生代谢产物合成的影响[J].植物研究,2018,38(3):391-398.Fan Y M,Dang S K,Wang W J,et al.Effect of carbon source,auxin and elicitor on the growth and synthesis of secondary metabolites of adventitious roots of Cajanus cajan(Linn.)Millsp.[J].Bulletin of Botanical Research,2018,38(3):391-398.
12.Mir S A,Bhat A S,Ahangar A A.Optimized in vitro methods for generating superoxide anion from hydroxylamine,and their response to flavonoids,curcumin and ascorbic acid[J].International Journal of Pharmtech Research,2013,5(2):349-358.
13.Zhang X X,Li C J,Nan Z B.Effects of cadmium stress on growth and anti-oxidative systems in Achnatherum inebrians,symbiotic with Neotyphodium gansuense[J].Journal of Hazardous Materials,2010,175(1-3):703-709.
14.Zhao J,Hu Q,Guo Y Q,et al.Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+influx and the oxidative burst[J].Plant Science,2001,161(3):423-431.
15.La G X,Liu G S,Ping F.Effects of monosodium glutamate wastewater on yield and quality of tomato fruit,SOD,CAT,POD activities and MDA content in tomato leaves[C].//Proceedings of the 2011 2nd international conference on mechanic automation and control engineering.Hohhot,China:IEEE,2011:6464-6467.
16.张宜奎,宋秀凯,刘爱英,等.Cd2+胁迫对文蛤鳃组织SOD、CAT活性及MDA含量的影响[J].安全与环境学报,2011,11(6):19-23.Zhang Y K,Song X K,Liu A Y,et al.Effects of Cd2+on SOD activity CAT activity,and MDA content in the gill of Meretrix meretrix[J].Journal of Safety and Environment,2011,11(6):19-23.
17.Hashemi S M,Naghavi M R.Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L.using abiotic elicitors[J].Plant Cell,Tissue and Organ Culture(PCTOC),2016,125(1):31-41.
18.Sirikantaramas S,Yamazaki M,Saito K.Mechanisms of resistance to self-produced toxic secondary metabolites in plants[J].Phytochemistry Reviews,2008,7(3):467-477.
19.孟书亦,马秀杰,韩梅,等.水杨酸对黄芩愈伤组织抗氧化酶活性及黄芩苷含量的影响[J].分子植物育种,2017,15(10):4179-4183.Meng S Y,Ma X J,Han M,et al.Effects of salicylic acid on antioxidant enzyme activity and content of baicalin in Scutellaria baicalensis callus[J].Molecular Plant Breeding,2017,15(10):4179-4183.
20.Kim O T,Bang K H,Kim Y C,et al.Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate[J].Plant Cell,Tissue and Organ Culture(PC-TOC),2009,98(1):25-33.
21.Song S H,Byun S Y.Characterization of cell growth and camptothecin production in cell cultures of Camptotheca acuminata[J].Journal of Microbiology&Biotechnology,1998,8(6):631-638.
22.Li J,Wang J,Li J X,et al.Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.:bioactive compounds and antioxidant enzymes[J].Research on Chemical Intermediates,2016,42(2):1503-1519.
23.Li T T,Hu Y Y,Du X H,et al.Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv.merrillii seedlings by activating photosynthesis and enhancing antioxidant systems[J].PLo S One,2014,9(10):e109492.
24.赵晓菊,张丽霞,满秀玲.NO对盐胁迫下长春花种子萌发和幼苗生理代谢的影响[J].植物研究,2018,38(5):669-674.Zhao X J,Zhang L X,Man X L.Effects of exogenous NOon seed germination and physiological metabolism in Catharanthus roseus seedling under Na Cl stress.Bulletin of Botanical Research,2018,38(5):669-674.
25.Farooq M A,Gill R A,Islam F,et al.Methyl jasmonate regulates antioxidant defense and suppresses arsenic uptake in Brassica napus L.[J].Frontiers in Plant Science,2016,7:468.
26.Li J X,Wang J,Wu X L,et al.Jasmonic acid and Methyl dihydrojasmonate enhance saponin biosynthesis as well as expression of functional genes in adventitious roots of Panax notoginseng F.H.Chen[J].Biotechnology and Applied Biochemistry,2017,64(2):225-238.