右旋糖酐酶开发及应用研究进展
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摘要
右旋糖酐酶是一类能特异性催化右旋糖酐中α-1,6-糖苷键水解的酶,其作用底物——右旋糖酐则是蔗糖经某些微生物发酵生成的高分子葡萄糖聚合物。右旋糖酐被广泛应用于医药、食品、材料等领域,但也给口腔健康、制糖生产等带来不良影响。随着人们对右旋糖酐、右旋糖酐水解物及其他多糖研究的深入,右旋糖酐酶发挥着越来越重要的作用,但右旋糖酐酶制剂的整体开发水平仍不高,应用深度仍有限。综述了右旋糖酐酶菌种构建、发酵、纯化、固定化、酶学性质表征、酶活性增强等多个方面的开发研究,以及右旋糖酐酶在制糖、食品、医药、材料等领域的应用研究进展,其中包括本团队在开发和应用方面的研究成果,讨论了在开发和应用过程中存在的问题,并对未来的开发和应用研究方向进行了展望。
Dextranase is a kind of enzyme that can specifically catalyzes the hydrolysis of α-1,6-glucosidic bonds on dextran. Dextran,the substrate of dextranase,is a glucan produced by the fermentation of sucrose via some microorganisms. Dextran is widely used in medicine,food,materials and other fields,but it brings the adverse effects on oral health,sugar production,etc. Studies on dextran,dextran hydrolysates and other polysaccharides are getting increasingly advanced and dextranase plays an increasingly important role;but the overall development level of dextranase preparation is still not high and its application is still limited. This review summarizes the development and researches on the construction of dextranase-producing strains,fermentation and purification of dextranase,dextranase immobilization,characterization of enzymatic properties,enzyme activity enhancement,etc.,as well as the research progresses on applying dextranase in sugar production,food,medicine and materials,including the research results in our team. Then the paper discusses the issues in the development and application and prospects the future research and development directions.
Dextranase is a kind of enzyme that can specifically catalyzes the hydrolysis of α-1,6-glucosidic bonds on dextran. Dextran,the substrate of dextranase,is a glucan produced by the fermentation of sucrose via some microorganisms. Dextran is widely used in medicine,food,materials and other fields,but it brings the adverse effects on oral health,sugar production,etc. Studies on dextran,dextran hydrolysates and other polysaccharides are getting increasingly advanced and dextranase plays an increasingly important role;but the overall development level of dextranase preparation is still not high and its application is still limited. This review summarizes the development and researches on the construction of dextranase-producing strains,fermentation and purification of dextranase,dextranase immobilization,characterization of enzymatic properties,enzyme activity enhancement,etc.,as well as the research progresses on applying dextranase in sugar production,food,medicine and materials,including the research results in our team. Then the paper discusses the issues in the development and application and prospects the future research and development directions.
引文
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[9]梁达奉,黄曾慰,曾练强,等.α-葡聚糖酶在毕赤酵母中的组成型表达[J].华南理工大学学报:自然科学版, 2012, 40(5):96-100.
[10]黄曾慰,梁达奉,曾练强,等.朱黄青霉α-葡聚糖酶在毕赤酵母中的高效表达[J].广西科学, 2014(6):614-618.
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[12] Wang X, Lu M, Wang S, et al. The atmospheric and roomtemperature plasma(ARTP)method on the dextranase activity and structure[J]. International Journal of Biological Macromolecules, 2014, 70(8):284-291.
[13] Jiao YL, Wang SJ, Lv MS, et al. Characterization of a marinederived dextranase and its application to the prevention of dental caries[J]. J Ind Microbiol Biotechnol, 2014, 41(1):17-26.
[14] Cai R, Lu M, Fang Y, et al. Screening, production, and characterization of dextranase from Catenovulum sp.[J]. Annals of Microbiology, 2014, 64(1):147-155.
[15] Kiran T, Asad W, et al. Industrially important hydrolytic enzyme diversity explored in stove ash bacterial isolates[J]. Pakistan Journal of Pharmaceutical Sciences, 2015, 28(6):2035-2040.
[16] Zohra RR, Aman A, Ansari A, et al. Purification, characterization and end product analysis of dextran degrading endodextranase from Bacillus licheniformis KIBGE-IB25[J]. International Journal of Biological Macromolecules, 2015, 78:243-248.
[17] Zhang YQ, Li RH, Zhang HB, et al. Purification, characterization,and application of a thermostable dextranase from Talaromyces pinophilus[J]. J Ind Microbiol Biotechnol, 2017, 44(2):317-327.
[18] Picozzi C, Meissner D, Chierici M, et al. Phage-mediated transfer of a dextranase gene in Lactobacillus sanfranciscensis and characterization of the enzyme[J]. International Journal of Food Microbiology, 2015, 202:48-53.
[19] Kim JK, Shin SY, Moon JS, et al. Isolation of dextran-hydrolyzing intestinal bacteria and characterization of their dextranolytic activities[J]. Biopolymers, 2015, 103(6):321-327.
[20] Sarbini SR, Kolida S, Naeye T, et al. In vitro fermentation of linear andα-1, 2-branched dextrans by the human fecal microbiota[J].Appl Environ Microbiol, 2011, 77(15):5307-5315.
[21] Olano-Martin E, Mountzouris KC, Gibson GR, et al. In vitro fermentability of dextran, oligodextran and maltodextrin by human gut bacteria[J]. Br J Nutr, 2000, 83(3):247-255.
[22] Goffin D, Delzenne N, Blecker C, et al. Will isomaltooligosaccharides, a well-established functional food in Asia,break through the european and American market? The status of knowledge on these prebiotics[J]. Critical Reviews in Food Science&Nutrition, 2011, 51(5):394-409.
[23]吴兆鹏,梁达奉,曾练强,等.毕赤酵母产α-葡聚糖酶发酵工艺研究[J].粮油食品科技, 2010, 18(2):29-31.
[24]梁达奉,吴兆鹏,曾练强,等.重组毕赤酵母产α-葡聚糖酶甲醇流加控制的研究[J].甘蔗糖业, 2011(5):21-27.
[25]吴兆鹏,曾练强,黄曾慰,等.重组毕赤酵母发酵甘油制备α-葡聚糖酶的研究[J].广西科学, 2014(6):624-628.
[26]吴兆鹏,梁达奉,黄曾慰,等.葡萄糖对组成型毕赤酵母发酵产α-葡聚糖酶的影响[J].广西糖业, 2015(6):16-21.
[27]黄曾慰,吴兆鹏,常国炜,等.重组毕赤酵母发酵生产α-葡聚糖酶的放大研究[J].甘蔗糖业, 2016(2):20-26.
[28]曹研研,张洪斌,李若菡,等.棘孢青霉菌发酵产右旋糖酐酶的条件优化[J].食品科学, 2015, 36(23):215-220.
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