转cry1Ie基因抗虫玉米对根际微生物群落碳代谢的影响
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摘要
转基因作物对土壤生态系统的影响日益引起人们的广泛关注。采用Biolog ECO微平板对转基因抗虫玉米IE09S034及其对应的受体玉米根际微生物群落碳代谢功能进行了比较研究。结果表明,转基因抗虫玉米IE09S034对根际微生物碳代谢功能无显著性影响,且发现年份和生育期是引起根际微生物碳代谢情况变化的主要因素。本文为转基因抗虫玉米IE09S034的环境安全性评估提供了新的依据。
With the increase of genetically modified( GM) crops area,their biosafety for soil ecosystems has become a controversial issue. This paper compared the functional diversity of rhizosphere microbial communities between GM Bt maize IE09S034 and its non-GM parental maize Zong31 by Biolog ECO microplate. Results indicated that there was no difference between IE09S034 and Zong31 in the functional diversity of microbial communities in rhizosphere soil. Besides,year and plant growth stage had stronger effect than cultivar. It was concluded that GM maize IE09S034 did not alter the functional diversity of microbial communities in rhizosphere soil.
With the increase of genetically modified( GM) crops area,their biosafety for soil ecosystems has become a controversial issue. This paper compared the functional diversity of rhizosphere microbial communities between GM Bt maize IE09S034 and its non-GM parental maize Zong31 by Biolog ECO microplate. Results indicated that there was no difference between IE09S034 and Zong31 in the functional diversity of microbial communities in rhizosphere soil. Besides,year and plant growth stage had stronger effect than cultivar. It was concluded that GM maize IE09S034 did not alter the functional diversity of microbial communities in rhizosphere soil.
引文
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[21]Liang J,Sun S,Ji J,et al..Comparison of the rhizosphere bacterial communities of zigongdongdou soybean and a highmethionine transgenic line of this cultivar[J].PLoS ONE,2014,9(7):e103343.
[22]Liang J,Xin L,Meng F,et al..High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms[J].Plant Soil Environ.,2016,62(10):441-446.
[23]Lv Y,Cai H,Yu J,et al..Biosafety assessment of GFPtransplastomic tobacco to rhizosphere microbial community[J].Ecotoxicology,2014,23(4):718-725.
[24]Wang G H,Jin J,Chen X L,et al..Biomass and catabolic diversity of microbial communities with long-term restoration,bare fallow and cropping history in Chinese Mollisols[J].Plant Soil Environ.,2007,53(4):177-185.
[25]Filion M.Do transgenic plants affect rhizobacteria populations[J].Microb.Biotechnol.,2008,1(6):463-475.
[26]汪海静.土壤微生物多样性的主要影响因素[J].北方环境,2011,23(1-2):90-91.Wang H J.The main affecting factors of soil microbial diversity[J].Northern Environ.,2011,23(1-2):90-91.
[27]Velasco A G V,Kowalchuk G A,Manero F J G,et al..Increased microbial activity and nitrogen mineralization coupled to changes in microbial community structure in the rhizosphere of Bt corn[J].Appl.Soil Ecol.,2013,68(2):46-56.
[28]叶慧香,崔跃原,宋新元,等.转cry1Ie基因抗虫玉米对土壤中细菌群落结构的影响[J].生物安全学报,2015,24(1):64-71.Ye H,Cui Y,Song X,et al..The effect of transgenic maize with cry1Ie gene on rhizosphere bacteria community structure[J].J.Biosafety,2015,24(1):64-71.
[29]刘新颖,王柏凤,周琳,等.转cry1Ie基因抗虫玉米IE09S034种植对田间大型土壤动物多样性的影响[J].作物杂志,2016(1):62-68.Liu X,Wang B,Zhou L,et al..Effect of cry1Ie on soil macrofauna diversity in transgenic corn IE09S034 fields[J].Crops,2016(1):62-68.
[2]Mina U,Khan S A,Choudhary A,et al..An approach for impact assessment of transgenic plants on soil ecosystem[J].Appl.Ecol.Environ.Res.,2008,6(3):1-19.
[3]Ali B,Sabri A N,Ljung K,et al..Auxin production by plant associated bacteria:Impact on endogenous IAA content and growth of Triticum aestivum L.[J].Lett.Appl.Microbiol.,2009,48(5):542-547.
[4]Castiglioni P,Warner D,Bensen R J,et al..Bacterial RNAchaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions[J].Plant Physiol.,2008,147(2):446-455.
[5]Innerebner G,Knief C,Vorholt J A.Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system[J].Appl.Environ.Microb.,2011,77(10):3202-3210.
[6]Whitman W B,Coleman D C,Wiebe W J.Prokaryotes:The unseen majority[J].P.Natl.Acad.Sci.USA,1998,95(12):6578-6583.
[7]Inceoglu O,Salles J F,Overbeek L V,et al..Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields[J].Appl.Environ.Microb.,2010,76(11):3675-3684.
[8]Peiffer J A,Spor A,Koren O,et al..Diversity and heritability of the maize rhizosphere microbiome under field conditions[J].P.Natl.Acad.Sci.USA,2013,110(16):6548-6553.
[9]梁晋刚,孟芳,张正光.转基因高蛋氨酸大豆对根际土壤主要有机元素和酶活性的影响[J].生物安全学报,2017,26(4):301-306.Liang J,Meng F,Zhang Z.Effect of a high-methionine transgenic soybean(Glycine max)on soil organic elements and enzyme activities in the rhizosphere[J].J.Biosafety,2017,26(4):301-306.
[10]梁晋刚,张正光.转基因高蛋氨酸大豆对根际真菌群落碳代谢功能的影响[J].江苏农业学报,2017,33(5):993-997.Liang J,Zhang Z.Effect of a high-methionine transgenic soybean line on functional diversity of rhizosphere fungal communities[J].Jiangsu J.Agric.Sci.,2017,33(5):993-997.
[11]Griffiths B S,Geoghegan I E,Robertson W M.Testing genetically engineered potato,producing the lectins GNA and Con A,on non-target soil organisms and processes[J].J.Appl.Ecol.,2000,37(1):159-170.
[12]Heuer H,Kroppenstedt R M,Lottmann J,et al..Effects of T4lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors[J].Appl.Environ.Microb.,2002,68(3):1325-1335.
[13]Wei M,Tan F,Zhu H,et al..Impact of Bt-transgenic rice(SHK601)on soil ecosystems in the rhizosphere during crop development[J].Plant Soil Environ.,2012,58(5):217-223.
[14]梁晋刚,张秀杰.转基因作物对土壤微生物多样性影响的研究方法[J].生物技术通报,2017,33(10):111-116.Liang J,Zhang X.Strategies for evaluating the effects of transgenic crops on soil microbial diversity[J].Biotech.Bull.,2017,33(10):111-116.
[15]Dai P,Jia H,Geng L,et al..Bt toxin Cry1Ie causes no negative effects on survival,pollen consumption,or olfactory learning in worker honey bees(Hymenoptera:Apidae)[J].J.Econ.Entomol.,2016,109(3):1028-1033.
[16]Feng D,Chen Z,Wang Z,et al..Domain III of Bacillus thuringiensis Cry1Ie toxin plays an important role in binding to peritrophic membrane of Asian corn borer[J].PLoS ONE,2015,10(8):e0136430.
[17]Guo J,He K,Hellmich R L,et al..Field trials to evaluate the effects of transgenic cry1Ie maize on the community characteristics of arthropod natural enemies[J].Sci.Rep.,2016,6:22102.
[18]Jia H,Geng L,Li Y,et al..The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica(Hymenoptera:Apidae)[J].Sci.Rep.,2016,6:24664.
[19]Song F,Zhang J,Gu A,et al..Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene[J].Appl.Environ.Microb.,2003,69(9):5207-5211.
[20]Liang J,Meng F,Sun S,et al..Community structure of arbuscular mycorrhizal fungi in rhizospheric soil of a transgenic high-methionine soybean and a near isogenic variety[J].PLoSONE,2015,10(12):e0145001.
[21]Liang J,Sun S,Ji J,et al..Comparison of the rhizosphere bacterial communities of zigongdongdou soybean and a highmethionine transgenic line of this cultivar[J].PLoS ONE,2014,9(7):e103343.
[22]Liang J,Xin L,Meng F,et al..High-methionine soybean has no adverse effect on functional diversity of rhizosphere microorganisms[J].Plant Soil Environ.,2016,62(10):441-446.
[23]Lv Y,Cai H,Yu J,et al..Biosafety assessment of GFPtransplastomic tobacco to rhizosphere microbial community[J].Ecotoxicology,2014,23(4):718-725.
[24]Wang G H,Jin J,Chen X L,et al..Biomass and catabolic diversity of microbial communities with long-term restoration,bare fallow and cropping history in Chinese Mollisols[J].Plant Soil Environ.,2007,53(4):177-185.
[25]Filion M.Do transgenic plants affect rhizobacteria populations[J].Microb.Biotechnol.,2008,1(6):463-475.
[26]汪海静.土壤微生物多样性的主要影响因素[J].北方环境,2011,23(1-2):90-91.Wang H J.The main affecting factors of soil microbial diversity[J].Northern Environ.,2011,23(1-2):90-91.
[27]Velasco A G V,Kowalchuk G A,Manero F J G,et al..Increased microbial activity and nitrogen mineralization coupled to changes in microbial community structure in the rhizosphere of Bt corn[J].Appl.Soil Ecol.,2013,68(2):46-56.
[28]叶慧香,崔跃原,宋新元,等.转cry1Ie基因抗虫玉米对土壤中细菌群落结构的影响[J].生物安全学报,2015,24(1):64-71.Ye H,Cui Y,Song X,et al..The effect of transgenic maize with cry1Ie gene on rhizosphere bacteria community structure[J].J.Biosafety,2015,24(1):64-71.
[29]刘新颖,王柏凤,周琳,等.转cry1Ie基因抗虫玉米IE09S034种植对田间大型土壤动物多样性的影响[J].作物杂志,2016(1):62-68.Liu X,Wang B,Zhou L,et al..Effect of cry1Ie on soil macrofauna diversity in transgenic corn IE09S034 fields[J].Crops,2016(1):62-68.