分子标记及RAD测序辅助选择改造两系不育系水稻粒型
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摘要
长粒型稻米具有垩白度低,直链淀粉含量高,蒸煮后坚韧蓬松的优点。为了改良水稻粒型,本研究以‘R15’为长粒型基因供体材料,‘Y58S’光温敏两系不育系作为轮回亲本进行改良,开发出了与控制粒型的qGL7紧密连锁的分子标记R072774。利用该分子标记在每一代选出含有长粒型基因的株系进行建库测序,利用RAD重测序数据进行基因组选择,选择含有长粒型基因且基因组背景最接近轮回亲本‘Y58S’的个体。从F2代开始进行回交,经过4代回交和1代自交,F2代及回交后代与‘Y58S’遗传背景平均相似率为F2(37.07%) Long grain rice has the advantages of low degree of chalkiness, high content of amylose and firm and fluffy after cooking. In order to improve the rice grain shape, in this study, 'R15' was used as long-grained gene donor material and 'Y58 S', photo-thermo-sensitive two-line sterile line, was used as a recurrent parent to develop a mo lecular marker R072774 closely linked to qGL7 controlling grain shape. Using this molecular marker, the individuals containing the long-grained gene were selected in each generation for the establishment of the genomic library, and the genome selection was performed using RAD resequencing data. The individuals containing the long-grained gene and the genome background closest to the recurrent parent 'Y58 S' were selected. Backcrossing from the F2 generation, after 4 generations of backcross and 1 generation of self-crossing, the average genetic background similarity of F2 generation and backcross generations compared with 'Y58 S' was F2(37.07%)
引文
Bai X.F.,Luo L.J.,Yan W.H.,Kovi M.R.,Zhan W.,and Xing Y.Z.,2010,Genetic dissection of rice grain shape using a recombinant inbred line population derived from two contrasting parents and fine mapping a pleiotropic quantitative trait locus qGL7,BMC Genet.,11:16
Fan C.C.,Yu S.B.,Wang C.R.,and Xing Y.H.,2009,A causal C-A mutation in the second exon of GS3 highly associated with rice grain length and validated as a functional marker,Theor.Appl.Genet.,118(3):465-472
Jiang L.R.,and Fang X.J.,2004,Improvement of the appearance traits of elite rice Zhenshan 97B by molecular introgression of targeted about 800 kb Interval from Indica rice jiafuzhan genome,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),2(3):453-454(江良荣,方宣钧,2004,分子标记辅助渗入佳辐占基因组约800 kb区间定向改良珍汕97B外观品质,分子植物育种,2(3):453-454)
Kovach M.J.,Sweeney M.T.,and McCouch S.R.,2007,New insights into the history of rice domestication,Trends Genet.,23(11):578-587
Li H.J.,Li P.,and Gao F.Y.,Lu X.J.,and Ren G.J.,2004,Improvement of amylose content of G46B by SSR marker-assisted selection,Zuowu Xuebao(Acta Agronomica Sinica),30(11):1159-1163(李浩杰,李平,高方远,陆贤军,任光俊,2004,SSR标记辅助选择改良冈46B直链淀粉含量的研究,作物学报,30(11):1159-1163)
Li J.,Thomson M.,and McCouch S.R.,2004,Fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3,Genetics,168(4):2187-2195
Li Y.B.,Fan C.C.,Xing Y.Z.,Jiang Y.H.,Luo L.J.,Sun L.,Shao D.,Xu C.J.,Li X.H.,Xiao J.H.,He Y.Q.,and Zhang Q.,2011,Natural variation in GS5 plays an important role in regulating grain size and yield in rice,Nat.Genet.,43(12):1266-1269
Mao H.L.,Sun S.Y.,Yao J.L.,Wang C.R.,Yu S.B.,Xu C.G.,Li X.H.,and Zhang Q.F.,2010,Linking differential domain functions of the GS3 protein to natural variation of grain size in rice,Proc.Natl.Acad.Sci.USA,107(45):19579-19584
Qiu X.J.,Du B.,Hu H.,Ou X.X.,LüW.K.,Lang L.W.,Xing D.Y.,Xu J.L.,Zheng T.Q.,Xu J.Y.,Li Z.X.,Zhang Y.B.,and Wang X.Y.,2017,Scanning QTLs for grain shape using two sets of introgression lines in rice,Int.J.Agric.Biol.,19:509-516
Qiu X.J.,Yuan Z.H.,He W.J.,Liu H.,Xu J.L.,and Xing D.Y.,2014,Genetic research of grain size traits in rice,Jiangxi Nongye Daxue Xuebao(Acta Agricultural Universitatis Jiangxiensis),36(4):705-709(邱先进,袁志华,何文静,刘环,徐建龙,邢丹英,2014,水稻粒型性状遗传研究进展,江西农业大学学报,36(4):705-709)
Sang T.,and Ge S.,2007,Genetics and phylogenetics of rice domestication,Curr.Opin.Genet.Dev.,17:533-538
Unnevehr L.J.,Duff B.,Juliano and B.O.,eds.,1992,Consumer demand for rice grain quality:introduction and major findings,The Philippines/International Development Research Centre,Ottawa,Canada,pp.5-19
Wan X.Y.,Wan J.M.,Jiang L.,Wang J.K.,Zhai H.Q.,Weng J.F.,Wang H.L.,Lei C.L.,Wang J.L.,Zhang X.,Cheng Z.J.,and Guo X.P.,2006,QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects,Theor.Appl.Genet.,112(7):1258-1270
Wang S.K.,Wu K.,Yuan Q.B.,Liu X.Y.,Liu Z.B.,Lin X.Y.,Zeng R.Z.,Zhu H.T.,Dong G.J.,Qian Q.,Zhang G.Q.,and Fu X.D.,2012,Control of grain size,shape and quality by OsSPL16 in rice,Nat.Genet.,44(8):950-954
Xing Z.,Tan F.,Hua P.,Sun L.,Xu G.,and Zhang Q.Q.,2002,Characterization of the main effects,epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice,Theor.Appl.Genet.,105(1-2):248-257
Yang Q.,Liang C.,Zhuang W.,Li J.,Deng H.,Deng Q.,and Wang B.,2007,Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping,Planta,225(2):321-330
Yin D.S.,Xia M.Y.,Li J.B.,Wan B.L.,Zha Z.P.,Du X.S.,and Qi H.X.,2011,Development of the STS linkage marker for rice blast resistance gene Pi9 and its application in molecular marker assisted breeding,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),25(1):25-30(殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄,2011,抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用,中国水稻科学,25(1):25-30)
Yu S.B.,Li J.X.,Xu C.G.,Tan Y.F.,Gao Y.J.,Li X.H.,Zhang Q.,and Saghai Maroof M.A.,1997,Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid,Proc.Natl.Acad.Sci.USA,94(17):9226-9231
Zhang F.J.,Zhang M.L.,and Zhu S.F.,2004,An improved rapid method of plant total DNA extraction,Zhiwu Jianyi(Plant Quarantine),18(6):330-332(张凤娟,张满良,朱水芳,2004,一种改进的水稻总DNA的快速提取方法,植物检疫,18(6):330-332)
Zhou L.J.,Sheng W.T.,Wu J.,Deng Q.Y.,and Zhao X.Q.,2015,Analysis of grain quality for state-registered indica hybrid rice varieties in China,Zajiao Shuidao(Hybrid Rice),30(3):76-80(周丽洁,盛文涛,吴俊,邓启云,赵向前,2015,国审杂交籼稻组合的稻米品质分析,杂交水稻,30(3):76-80)
Fan C.C.,Yu S.B.,Wang C.R.,and Xing Y.H.,2009,A causal C-A mutation in the second exon of GS3 highly associated with rice grain length and validated as a functional marker,Theor.Appl.Genet.,118(3):465-472
Jiang L.R.,and Fang X.J.,2004,Improvement of the appearance traits of elite rice Zhenshan 97B by molecular introgression of targeted about 800 kb Interval from Indica rice jiafuzhan genome,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),2(3):453-454(江良荣,方宣钧,2004,分子标记辅助渗入佳辐占基因组约800 kb区间定向改良珍汕97B外观品质,分子植物育种,2(3):453-454)
Kovach M.J.,Sweeney M.T.,and McCouch S.R.,2007,New insights into the history of rice domestication,Trends Genet.,23(11):578-587
Li H.J.,Li P.,and Gao F.Y.,Lu X.J.,and Ren G.J.,2004,Improvement of amylose content of G46B by SSR marker-assisted selection,Zuowu Xuebao(Acta Agronomica Sinica),30(11):1159-1163(李浩杰,李平,高方远,陆贤军,任光俊,2004,SSR标记辅助选择改良冈46B直链淀粉含量的研究,作物学报,30(11):1159-1163)
Li J.,Thomson M.,and McCouch S.R.,2004,Fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3,Genetics,168(4):2187-2195
Li Y.B.,Fan C.C.,Xing Y.Z.,Jiang Y.H.,Luo L.J.,Sun L.,Shao D.,Xu C.J.,Li X.H.,Xiao J.H.,He Y.Q.,and Zhang Q.,2011,Natural variation in GS5 plays an important role in regulating grain size and yield in rice,Nat.Genet.,43(12):1266-1269
Mao H.L.,Sun S.Y.,Yao J.L.,Wang C.R.,Yu S.B.,Xu C.G.,Li X.H.,and Zhang Q.F.,2010,Linking differential domain functions of the GS3 protein to natural variation of grain size in rice,Proc.Natl.Acad.Sci.USA,107(45):19579-19584
Qiu X.J.,Du B.,Hu H.,Ou X.X.,LüW.K.,Lang L.W.,Xing D.Y.,Xu J.L.,Zheng T.Q.,Xu J.Y.,Li Z.X.,Zhang Y.B.,and Wang X.Y.,2017,Scanning QTLs for grain shape using two sets of introgression lines in rice,Int.J.Agric.Biol.,19:509-516
Qiu X.J.,Yuan Z.H.,He W.J.,Liu H.,Xu J.L.,and Xing D.Y.,2014,Genetic research of grain size traits in rice,Jiangxi Nongye Daxue Xuebao(Acta Agricultural Universitatis Jiangxiensis),36(4):705-709(邱先进,袁志华,何文静,刘环,徐建龙,邢丹英,2014,水稻粒型性状遗传研究进展,江西农业大学学报,36(4):705-709)
Sang T.,and Ge S.,2007,Genetics and phylogenetics of rice domestication,Curr.Opin.Genet.Dev.,17:533-538
Unnevehr L.J.,Duff B.,Juliano and B.O.,eds.,1992,Consumer demand for rice grain quality:introduction and major findings,The Philippines/International Development Research Centre,Ottawa,Canada,pp.5-19
Wan X.Y.,Wan J.M.,Jiang L.,Wang J.K.,Zhai H.Q.,Weng J.F.,Wang H.L.,Lei C.L.,Wang J.L.,Zhang X.,Cheng Z.J.,and Guo X.P.,2006,QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects,Theor.Appl.Genet.,112(7):1258-1270
Wang S.K.,Wu K.,Yuan Q.B.,Liu X.Y.,Liu Z.B.,Lin X.Y.,Zeng R.Z.,Zhu H.T.,Dong G.J.,Qian Q.,Zhang G.Q.,and Fu X.D.,2012,Control of grain size,shape and quality by OsSPL16 in rice,Nat.Genet.,44(8):950-954
Xing Z.,Tan F.,Hua P.,Sun L.,Xu G.,and Zhang Q.Q.,2002,Characterization of the main effects,epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice,Theor.Appl.Genet.,105(1-2):248-257
Yang Q.,Liang C.,Zhuang W.,Li J.,Deng H.,Deng Q.,and Wang B.,2007,Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping,Planta,225(2):321-330
Yin D.S.,Xia M.Y.,Li J.B.,Wan B.L.,Zha Z.P.,Du X.S.,and Qi H.X.,2011,Development of the STS linkage marker for rice blast resistance gene Pi9 and its application in molecular marker assisted breeding,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),25(1):25-30(殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄,2011,抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用,中国水稻科学,25(1):25-30)
Yu S.B.,Li J.X.,Xu C.G.,Tan Y.F.,Gao Y.J.,Li X.H.,Zhang Q.,and Saghai Maroof M.A.,1997,Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid,Proc.Natl.Acad.Sci.USA,94(17):9226-9231
Zhang F.J.,Zhang M.L.,and Zhu S.F.,2004,An improved rapid method of plant total DNA extraction,Zhiwu Jianyi(Plant Quarantine),18(6):330-332(张凤娟,张满良,朱水芳,2004,一种改进的水稻总DNA的快速提取方法,植物检疫,18(6):330-332)
Zhou L.J.,Sheng W.T.,Wu J.,Deng Q.Y.,and Zhao X.Q.,2015,Analysis of grain quality for state-registered indica hybrid rice varieties in China,Zajiao Shuidao(Hybrid Rice),30(3):76-80(周丽洁,盛文涛,吴俊,邓启云,赵向前,2015,国审杂交籼稻组合的稻米品质分析,杂交水稻,30(3):76-80)