太行鸡慢羽公鸡羽速基因型鉴定方法的建立与验证
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摘要
快慢羽性状为伴性遗传性状,可通过建立雌雄自鉴别品系应用于家禽的早期性别鉴定。因慢羽基因对快羽基因呈显性,故区分慢羽公鸡的羽速基因型是建立慢羽纯系的重要工作。使用传统测交鉴定方法鉴别耗时费力,而已有的分子鉴定方法有的成本太高,有的准确率太低。本研究以已有表型记录的快羽或慢羽太行鸡公母鸡为试验材料,设计了半定量PCR鉴定公鸡慢羽羽速基因型引物,通过摸索最佳反应体系和条件并通过对测交验证,最终建立了能较准确鉴定太行鸡慢羽公鸡羽速基因型方法,为太行鸡慢羽纯系的建立奠定技术基础。
Slow and fast feathering traits are sex-linkage traits and could be used to identify the sex of poultry at early age.The slow feathering gene is dominant to the fast feathering one.So to authenticate the genotype of late featering male chicken is an important work for establishing late feathering chicken lines.It is time-consuming and laborious to identify the feathering-speed genotype of the slow-feather cock by using the method of traditional test cross.Therefore,Using the chickens known the feathering phenotypic(slow-or fast-feathering) as experiment material and refered to the related literature,semi-quantitative PCR primers were designed for identification feathering speed genotype.Through groping for the best reaction system and reaction conditions,a semi-quantitative PCR method was setted up and the accuracy of this method was tested by test cross.This research laid a solid foundation for the establishment of the late-feathing line of taihang chicken.
Slow and fast feathering traits are sex-linkage traits and could be used to identify the sex of poultry at early age.The slow feathering gene is dominant to the fast feathering one.So to authenticate the genotype of late featering male chicken is an important work for establishing late feathering chicken lines.It is time-consuming and laborious to identify the feathering-speed genotype of the slow-feather cock by using the method of traditional test cross.Therefore,Using the chickens known the feathering phenotypic(slow-or fast-feathering) as experiment material and refered to the related literature,semi-quantitative PCR primers were designed for identification feathering speed genotype.Through groping for the best reaction system and reaction conditions,a semi-quantitative PCR method was setted up and the accuracy of this method was tested by test cross.This research laid a solid foundation for the establishment of the late-feathing line of taihang chicken.
引文
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[4] 王翠苹,孙莉.绿壳蛋鸡快慢羽自别雌雄配套品系的建立方法[J].畜牧兽医科技信息,2011(2):76-77.
[5] 王海洲,赵纪华,贾晓晖.日照麻鸡快慢羽纯系的选育及自别雌雄效果分析[J].中国家禽,2016(9):54-56.
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[7] SMITH E J,LEVIN I.Application of a locusspecific DNA hybridization probe in the analysis of the slowfeathering endogenous virus complex of chickens[J].Poul Sci,1991,70(9):1957-1964.
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[10] 李培周,李华,杜炳旺,等.贵妃鸡羽速基因分子检测及相关早熟性状分析[J].中国家禽,2013,35(21):5-8.
[11] 李珊珊,李东华,吕福琨,等.慢羽系麒麟公鸡纯合个体分子检测方法的建立[J].河南农业科学,2016,45(7):118-121.
[12] 张乐超,王晗,张秀玲,等.鸡ev21占位区和未占位区重复序列结构与羽速表型关系研究[J].畜牧兽医学报,2017,28(6):990-999.
[13] ELFERINK M G,VALLéE A A,JUNGERIUS A P,et al.Partial duplication of the PRLR and SPEF2 genes at the latefeathering locus in chicken[J].BMC Genomics,2008,391(9):1-9.
[14] BU G X,HUANG G,FU H,et al.Characterization of the novel duplicated PRLR gene at the late-feathering K locus in Lohmann chickens[J].J Mol Endorinol,2013,51(2):261-276.
[15] ZHAO J C,YAO J H,LI F Y.Identification of candidate genes for chicken early-and late-feathering[J].Poul Sci,2016,95(7):1498-1503.
[2] 张秀玲,李兰会,李祥龙,等.河北太行鸡遗传资源调研报告[J].山东农业大学学报( 自然科学版),2015,46(5):725-729.
[3] 宋素芳,康相涛,孙桂荣,等.固始鸡快慢羽纯系的选育及自别雌雄效果研究[J].养殖与饲料,2004(2):4-6.
[4] 王翠苹,孙莉.绿壳蛋鸡快慢羽自别雌雄配套品系的建立方法[J].畜牧兽医科技信息,2011(2):76-77.
[5] 王海洲,赵纪华,贾晓晖.日照麻鸡快慢羽纯系的选育及自别雌雄效果分析[J].中国家禽,2016(9):54-56.
[6] BACON L D,SMITH E,CRITTENDEN L B,et al.Association of the slow feathering ( K) and an endogenous viral ( ev21) gene on the Z chromosome of chickens[J].Poul Sci,1988,67(2):191197.
[7] SMITH E J,LEVIN I.Application of a locusspecific DNA hybridization probe in the analysis of the slowfeathering endogenous virus complex of chickens[J].Poul Sci,1991,70(9):1957-1964.
[8] IRAQI F,SMITH E J.Determination of the zygosity of ev21-K in late-feathering male white leghorns using the polymerase chain reaction[J].Poul Sci,1994,73(7):939-946.
[9] IRAQI F,SMITH E J.Organization of the sex-linked late-feathering haplotype in chickens[J].Anim Gen,1995,26:141-146.
[10] 李培周,李华,杜炳旺,等.贵妃鸡羽速基因分子检测及相关早熟性状分析[J].中国家禽,2013,35(21):5-8.
[11] 李珊珊,李东华,吕福琨,等.慢羽系麒麟公鸡纯合个体分子检测方法的建立[J].河南农业科学,2016,45(7):118-121.
[12] 张乐超,王晗,张秀玲,等.鸡ev21占位区和未占位区重复序列结构与羽速表型关系研究[J].畜牧兽医学报,2017,28(6):990-999.
[13] ELFERINK M G,VALLéE A A,JUNGERIUS A P,et al.Partial duplication of the PRLR and SPEF2 genes at the latefeathering locus in chicken[J].BMC Genomics,2008,391(9):1-9.
[14] BU G X,HUANG G,FU H,et al.Characterization of the novel duplicated PRLR gene at the late-feathering K locus in Lohmann chickens[J].J Mol Endorinol,2013,51(2):261-276.
[15] ZHAO J C,YAO J H,LI F Y.Identification of candidate genes for chicken early-and late-feathering[J].Poul Sci,2016,95(7):1498-1503.