可降解PLLA/Mg-Zn-Y-Nd合金肠道支架在兔中的生物安全性探究
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摘要
目的探究解决肠道狭窄的经左旋聚乳酸(PLLA)涂层处理过的PLLA/镁-锌-钇-钕(Mg-Zn-Y-Nd)合金肠道支架在兔中的生物安全性。方法 36只新西兰大白兔,随机分为空白对照组、裸支架组、PLLA涂层组,各12只。在其肠道内植入不同处理的可降解Mg-Zn-Y-Nd合金支架。分别于植入后2、5、8 d进行X线、组织学观察,以了解植入支架的生物安全情况。结果 (1) X线检测:PLLA/Mg-Zn-Y-Nd支架植入兔肠道5～8 d期间,支架因降解致使支架结构塌陷而脱落。(2) HE染色:PLLA/Mg-Zn-Y-Nd支架植入兔内后,肝脏组织和肾脏组织的形态和结构均未出现明显异常。(3)免疫组化:术后2 d, PLLA涂层组Bax表达水平(17.3±1.6)%略高于空白对照组(17.0±1.3)%。裸支架组Bax表达水平(18.3±2.6)%略高于空白对照组,且裸支架组Bax表达水平稍高于PLLA涂层组。术后5 d,PLLA涂层组Bax表达水平(17.1±1.0)%较术后2 d稍降低,同时略高于空白对照组(16.6±1.2)%。但随着时间推移,裸支架组Bax的表达水平始终略高于PLLA涂层组和空白对照组。在Bcl-2的表达方面,随着支架植入时间延长,第5天PLLA涂层组表达水平(14.7±1.3)%较第2天(15.2±1.0)%稍降低,而且PLLA涂层组的Bcl-2表达水平在整个实验期间均略低于裸支架组和稍高于空白对照组。但裸支架组与空白对照组之间Bcl-2表达水平在实验期间比较差异均无统计学意义(P>0.05)。术后2、5 d后,不同组间Bax和Bcl-2的表达水平比较差异均无统计学意义(P>0.05)。结论 PLLA涂层的Mg-Zn-Y-Nd合金肠道支架未引起明显的炎症反应,而且对肠道上皮细胞增殖无明显促进作用,具有较好的生物学安全性。
引文
[1]刘小丽.血管支架用Mg-Zn-Y-Nd合金的体外生物相容性及表面改性研究.北京科技大学, 2015.
[2]程竑.浅谈医用镁及镁合金的应用特性及其表面改性.口腔材料器械杂志, 2018, 27(4):181-184.
[3]Hou SS, Zhang RR, Guan SK, et al. In vitro corrosion behavior of Ti-O film deposited on fluoride-treated Mg-Zn-Y-Nd alloy.Applied Surface Science, 2012, 258(8):3571-3577.
[4]Zhu SJ, Liu Q, Qian YF, et al. Effect of different processings on mechanical property and corrosion behavior in simulated body fluid of Mg-Zn-Y-Nd alloy for cardiovascular stent application.Frontiers of Materials Science, 2014, 8(3):256-263.
[5]Liu J, Wang P, Chu CC, et al. A novel biodegradable and biologically functional Arginine-based poly(ester urea urethane)coating for Mg-Zn-Y-Nd alloy:enhancement in corrosion-resistance and biocompatibility. Journal of Materials Chemistry B, 2017, 5(9):1787-1802.
[6]Liu J, Wang P, Chu CC, et al. Arginine-leucine based poly(ester urea urethane)coating for Mg-Zn-Y-Nd alloy in cardiovascular stent applications. Colloids&Surfaces B Biointerfaces, 2017,159:78.
[7]Song X, Chang L, Wang J, et al. Investigation on the in vitro cytocompatibility of Mg-Zn-Y-Nd-Zr alloys as degradable orthopaedic implant materials. J Mater Sci Mater Med, 2018,29(4):44.
[8]Wang J, Wang L, Guan S, et al. Microstructure and corrosion properties of as sub-rapid solidification Mg-Zn-Y-Nd alloy in dynamic simulated body fluid for vascular stent application. Journal of Materials Science Materials in Medicine, 2010, 21(7):2001-2008.
[9]谢旭升,李刚,李翼,等.生物医用纺织肠道支架研究进展.产业用纺织品, 2016, 34(10):1-10.
[10]周嘉敏,钟芸诗,姚礼庆.结直肠支架应用进展.中国实用外科杂志, 2013, 33(11):982-985.
[11]王心玲,胡桐楠,储冰峰.镁合金材料与人内皮细胞的生物相容性.中国组织工程研究, 2017, 21(18):2846-2851.
[2]程竑.浅谈医用镁及镁合金的应用特性及其表面改性.口腔材料器械杂志, 2018, 27(4):181-184.
[3]Hou SS, Zhang RR, Guan SK, et al. In vitro corrosion behavior of Ti-O film deposited on fluoride-treated Mg-Zn-Y-Nd alloy.Applied Surface Science, 2012, 258(8):3571-3577.
[4]Zhu SJ, Liu Q, Qian YF, et al. Effect of different processings on mechanical property and corrosion behavior in simulated body fluid of Mg-Zn-Y-Nd alloy for cardiovascular stent application.Frontiers of Materials Science, 2014, 8(3):256-263.
[5]Liu J, Wang P, Chu CC, et al. A novel biodegradable and biologically functional Arginine-based poly(ester urea urethane)coating for Mg-Zn-Y-Nd alloy:enhancement in corrosion-resistance and biocompatibility. Journal of Materials Chemistry B, 2017, 5(9):1787-1802.
[6]Liu J, Wang P, Chu CC, et al. Arginine-leucine based poly(ester urea urethane)coating for Mg-Zn-Y-Nd alloy in cardiovascular stent applications. Colloids&Surfaces B Biointerfaces, 2017,159:78.
[7]Song X, Chang L, Wang J, et al. Investigation on the in vitro cytocompatibility of Mg-Zn-Y-Nd-Zr alloys as degradable orthopaedic implant materials. J Mater Sci Mater Med, 2018,29(4):44.
[8]Wang J, Wang L, Guan S, et al. Microstructure and corrosion properties of as sub-rapid solidification Mg-Zn-Y-Nd alloy in dynamic simulated body fluid for vascular stent application. Journal of Materials Science Materials in Medicine, 2010, 21(7):2001-2008.
[9]谢旭升,李刚,李翼,等.生物医用纺织肠道支架研究进展.产业用纺织品, 2016, 34(10):1-10.
[10]周嘉敏,钟芸诗,姚礼庆.结直肠支架应用进展.中国实用外科杂志, 2013, 33(11):982-985.
[11]王心玲,胡桐楠,储冰峰.镁合金材料与人内皮细胞的生物相容性.中国组织工程研究, 2017, 21(18):2846-2851.