芩黄清肺散的药效学研究
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摘要
为了观察中药复方制剂芩黄清肺散的抗炎、止咳、化痰、平喘作用,试验将动物随机分为模型组、阳性药物组、芩黄清肺散高、中、低剂量组。通过二甲苯致小鼠耳廓肿胀模型法观察芩黄清肺散抗炎作用;通过小鼠浓氨水引咳法观察其止咳作用;通过小鼠气管酚红排泌法观察其祛痰作用;通过组织胺和乙酰胆碱豚鼠引喘模型观察其平喘作用。结果表明:芩黄清肺散能够降低二甲苯所致小鼠耳部肿胀,提高抑制率;能显著抑制浓氨水引起的咳嗽,延长咳嗽潜伏期,减少咳嗽次数;能促进小鼠气管内酚红的分泌,促进痰液的排出;哮喘试验中芩黄清肺散能延长豚鼠哮喘发作潜伏期。可见芩黄清肺散具有较好的止咳、祛痰、平喘、抗炎作用。
The aim of the present study was to observe the anti-inflammatory,antitussive,expectorant and antiasthmatic effects of the Traditional Chinese Medicine compound Qinhuang qingfei powder.The mice were randomly divided into model group,positive drug group,Qinhuang qingfei high,medium and low dose group in the experiment.The anti-inflammatory effect of Qinhuang qingfei powder was observed by using xylene-induced mouse auricle swelling model.The antitussive effect was observed by using the method of concentrated ammonia-induced mouse cough.The expectorant effect was observed by using the method of phenol red excretion in mouse trachea.The antasthmatic effect was observed by using the histamine and acetylcholine induced guinea pigs asthma model.The results showed that the ear swelling of the mice induced by xylene was reduced by using the Qinhuang qingfei powder,and the inhibition rate was increased.The chemical-induced cough by ammonia was significantly inhibited by using the Qinhuang qingfei powder.The incubation period of cough was extended and the number of cough was also reduced.The secretion of the mice tracheal phenol red was promoted by using the Qinhuang qingfei powder.And the sputum discharge was promoted.The incubation period of asthma in guinea pigs could be prolonged by using the Qinhuang qingfei powder in the Asthma test.The results indicated that the Qinhuang qingfei powder had an obvious effects of anti-inflammatory,antitussive,expectorant and antiasthmatic.
The aim of the present study was to observe the anti-inflammatory,antitussive,expectorant and antiasthmatic effects of the Traditional Chinese Medicine compound Qinhuang qingfei powder.The mice were randomly divided into model group,positive drug group,Qinhuang qingfei high,medium and low dose group in the experiment.The anti-inflammatory effect of Qinhuang qingfei powder was observed by using xylene-induced mouse auricle swelling model.The antitussive effect was observed by using the method of concentrated ammonia-induced mouse cough.The expectorant effect was observed by using the method of phenol red excretion in mouse trachea.The antasthmatic effect was observed by using the histamine and acetylcholine induced guinea pigs asthma model.The results showed that the ear swelling of the mice induced by xylene was reduced by using the Qinhuang qingfei powder,and the inhibition rate was increased.The chemical-induced cough by ammonia was significantly inhibited by using the Qinhuang qingfei powder.The incubation period of cough was extended and the number of cough was also reduced.The secretion of the mice tracheal phenol red was promoted by using the Qinhuang qingfei powder.And the sputum discharge was promoted.The incubation period of asthma in guinea pigs could be prolonged by using the Qinhuang qingfei powder in the Asthma test.The results indicated that the Qinhuang qingfei powder had an obvious effects of anti-inflammatory,antitussive,expectorant and antiasthmatic.
引文
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[14]高源.大黄酸在LPS活化巨噬细胞中发挥抗炎与促炎双向作用的机制研究[D].北京:北京协和医学院,2014.
[15]李晓红,齐云,蔡润兰,等.甘草总皂苷抗炎作用机制研究[J].中国实验方剂学杂志,2010,16(5):110-113.
[16]黎雪桂.枇杷叶主要提取物药理作用研究进展[J].中国当代医药,2012,19(11):17-18.
[17]叶广亿,李书渊,陈艳芬,等.枇杷叶不同提取物的止咳化痰平喘作用比较研究[J].中药药理与临床,2013,29(2):100-102.
[18]张明发,沈雅琴.甘草及其提取物对呼吸系统的药理作用[J].现代药物与临床,2010,25(4):262-267.
[19]丁亚春,刘娅,何小溪,等.黄芩苷与甘草甜素合用对豚鼠实验性哮喘的影响[J].中国实验诊断学,2007,11(3):366-368.
[2]牛建荣,张继瑜,周绪正,等.“菌毒清”口服液对人工感染鸡传染性喉气管炎的治疗[J].甘肃农业大学学报,2015,50(4):12-17,22.
[3]何永明,张浩吉,杨鸿,等.“黄芩、大黄”合剂对人工诱发传染性喉气管炎患鸡血液SOD、MDA水平的影响[J].中国家禽,2003,25(17):9-11.
[4]马四补,隋艳华,王绍辉,等.苗药果上叶镇咳、抗炎作用有效部位筛选[J].贵阳中医学院学报,2016,38(1):1-5.
[5]李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社,2005.
[6]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,2006.
[7]贾琳,冯启荣,苏静.中药百部的主要药效学观察[J].山东医学高等专科学校学报,2014,36(3):197-200.
[8]辛文妤,宋俊科,何国荣,等.黄芩素和黄芩苷的药理作用及机制研究进展[J].中国新药杂志,2013,22(6):647-653,659.
[9]刘菊福,卢长安,廖福龙,等.不同产地黄芩提取物主要药效作用的比较[J].中国中医药信息杂志,2001,8(3):28-30.
[10]WOO K J,LIM J H,SUH S I,et al.Differential inhibitory effects of baicalein and baicalin on LPS-induced cyclooxygenase-2 expression through inhibition of C/EBPbeta DNA-binding activity[J].Immunobiology,2006,211(5):359-368.
[11]KIM E H,SHIM B,KANG S,et al.Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules[J].J Ethnopharmacol,2009,126(2):320-331.
[12]YOON S B,LEE Y J,PARK S K,et al.Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7macrophages[J].J Ethnopharmacol,2009,125(2):286-290.
[13]HU B Y,ZHANG H,MENG X L,et al.Aloe-emodin from rhubarb(Rheum rhabarbarum)inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages[J].J Ethnopharmacol,2014,153(3):846-853.
[14]高源.大黄酸在LPS活化巨噬细胞中发挥抗炎与促炎双向作用的机制研究[D].北京:北京协和医学院,2014.
[15]李晓红,齐云,蔡润兰,等.甘草总皂苷抗炎作用机制研究[J].中国实验方剂学杂志,2010,16(5):110-113.
[16]黎雪桂.枇杷叶主要提取物药理作用研究进展[J].中国当代医药,2012,19(11):17-18.
[17]叶广亿,李书渊,陈艳芬,等.枇杷叶不同提取物的止咳化痰平喘作用比较研究[J].中药药理与临床,2013,29(2):100-102.
[18]张明发,沈雅琴.甘草及其提取物对呼吸系统的药理作用[J].现代药物与临床,2010,25(4):262-267.
[19]丁亚春,刘娅,何小溪,等.黄芩苷与甘草甜素合用对豚鼠实验性哮喘的影响[J].中国实验诊断学,2007,11(3):366-368.