摘要
为获悉云南省部分地区猫血巴尔通氏体的感染情况,采集来自云南省部分地区猫血液样品338份,PCR扩增猫血巴尔通氏体的16S rRNA基因,通过测序、序列比对分析、构建进化树,确定所检获的猫血巴尔通氏体种属关系。结果有21个样本确定为猫血巴尔通氏体感染阳性,总体感染率为6.21%(21/338),其中检测20份样品为猫小型血巴尔通氏体,1份样品为猫大型血巴尔通氏体。对不同养殖模式和不同年龄进行感染因素分析,表明感染率在不同养殖模式和不同年龄段之间有显著的差异(P<0.05)。本研究首次报道了云南省部分地区猫体内存在猫血巴尔通氏体的感染,为云南省猫血巴尔通氏体病的防控提供了数据参考。
The present study was to examine the prevalence of Haemobartonellafelisinfections in cats from some areas of Yunnan province,a total of 338 fresh blood were collected and was detected using PCR amplification of the 16S rRNA gene,and the product of PCR were sequenced and compared the phylogenetic analysis.Results showed that 21 samples were positive,and the overall prevalence of H.felis was 6.21%(21/338).Twenty samples were infected with Candidatus Mycoplasma haemominutum,and one sample was infected with Mycoplasma haemofelis.Analysis of influences factors showed that there was a significant difference between breeding mode and age(P<0.05).The present investigation firstly report of the H.felisinfection in cat in Yunnan and the study provide the basic data for preventing and controlling H.felisinfection in cats from Yunnan Province.
引文
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