基于灰色关联度不同产地硝石抗肿瘤作用谱效关系研究
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摘要
目的:探究不同产地硝石无机元素特征轮廓谱与抗肿瘤作用的谱效关系,为明确硝石抗肿瘤作用的物质基础提供理论依据。方法:采用电感耦合等离子体质谱法,建立不同产地硝石无机元素特征轮廓谱;以肿瘤细胞抑制率为药效学指标,采用灰色关联度分析法建立无机元素轮廓谱与抗肿瘤药效之间的谱效关系。结果:建立了5个不同产地硝石无机元素特征轮廓谱,得到Cu、Na、Mg、Zn、K、Ca、Se、Sb、Ba 9个差异元素,根据关联度计算结果,Cu、Ba、Na、Se、Ca5个元素与抗肿瘤药效关联度大于0.6。结论:明确了硝石中Cu、Ba、Na、Se、Ca是抗肿瘤作用的主要无机成分,为深入研究硝石抗肿瘤药效物质基础及建立基于抗肿瘤药效的硝石质量控制方法提供思路。
Objective:To explore the relationship between the characteristic spectrum of the inorganic elements and anti-tumor effects from different regions of saltpeter,provide theoretical foundation for clarifying the material basis of anti-tumor effects of saltpeter. Methods:ICP-MS was used to establish the characteristic spectrum of the inorganic elements from different regions;The tumor cell inhibition rate was taken as the pharmacodynamic index,the spectral relationship between the characteristic spectrum of the inorganic elements and the anti-tumor effects was established by grayscale correlation analysis. Results:The characteristic spectrum of the inorganic elements from 5 different regions were established,Nine different elements including Cu,Na,Mg,Zn,K,Ca,Se,Sb and Ba were obtained,according to the calculation results of correlation degree,the correlation between the five elements of Cu,Ba,Na,Se and Ca are more than 0.6. Conclusion:It is clear that Cu,Ba,Na,Se and Ca are the main medicinal components of anti-tumor effect in the saltpeter,provide ideas for further research on the anti-tumor substance basis and establishment quality control method based on anti-tumor effects of saltpeter.
Objective:To explore the relationship between the characteristic spectrum of the inorganic elements and anti-tumor effects from different regions of saltpeter,provide theoretical foundation for clarifying the material basis of anti-tumor effects of saltpeter. Methods:ICP-MS was used to establish the characteristic spectrum of the inorganic elements from different regions;The tumor cell inhibition rate was taken as the pharmacodynamic index,the spectral relationship between the characteristic spectrum of the inorganic elements and the anti-tumor effects was established by grayscale correlation analysis. Results:The characteristic spectrum of the inorganic elements from 5 different regions were established,Nine different elements including Cu,Na,Mg,Zn,K,Ca,Se,Sb and Ba were obtained,according to the calculation results of correlation degree,the correlation between the five elements of Cu,Ba,Na,Se and Ca are more than 0.6. Conclusion:It is clear that Cu,Ba,Na,Se and Ca are the main medicinal components of anti-tumor effect in the saltpeter,provide ideas for further research on the anti-tumor substance basis and establishment quality control method based on anti-tumor effects of saltpeter.
引文
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[21]李丽,古扎丽努尔·阿不力孜.微量元素与宫颈癌的研究进展[J].广东微量元素科学,2015,22(1):53-57.
[22]孟红旭,姚明江,刘建勋.人参皂苷Re对大鼠心室肌细胞钠、钾离子通道的影响[J].世界中医药,2013,8(10):1147-1149.
[23]Roderick H Llewelyn,Cook Simon J.Ca2+signalling checkpoints in cancer:remodelling Ca2+for cancer cell proliferation and survival[J].Nature Reviews Cancer,2008,8(5):361-375.
[2]田甜.经方硝类药物考辨及应用规律研究[D].北京:北京中医药大学,2010.
[3]刘庆芳.火硝的临床应用评价[J].河南大学学报:医学科学版,2004(1):67-68.
[4]邵江娟,吕翔,路长珍,等.硝石化学成分研究[J].南京中医药大学学报,2010,26(4):306-307,324.
[5]黎娟兰.11例中药火硝急性中毒的护理[J].中国医学创新,2009,6(35):130-131.
[6]池婕,林兵,刘志宏,等.基于最小二乘回归分析法的雷公藤多苷片免疫抑制作用谱效关系研究[J].中草药,2015,46(18):2755-2758.
[7]薛丹平,张美敬,余越,等.雷公藤不同乙醇提取物指纹图谱及与大鼠肾小球系膜细胞的谱效关系研究[J].中国药师,2017,20(4):617-620.
[8]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447-3452.
[9]王枚博,夏晶,王欣美,等.金银花等10种中药材中15种无机元素分布规律及其相关性研究[J].中国药学杂志,2012,47(8):620-625.
[10]金鹏飞,宋丽洁,胡欣,等.ICP-MS研究中药炮制前后18种微量元素总量和溶出特性的变化[J].中国药学杂志,2010,45(12):893-897.
[11]杨波,王振国.植物类中药寒热药性与无机元素相关性研究[J].南京中医药大学学报,2011,27(2):109-111.
[12]李毓雯,万小华,宁琴,等.铜过量负荷导致肝细胞凋亡及其对Bax Bcl-2基因表达的影响[J].中国当代儿科杂志,2008(1):42-46.
[13]韩尽斌,曲毅,徐新春,等.抗肿瘤Cu配合物研究进展[J].现代中西医结合杂志,2014,23(18):2034-2037.
[14]朱敬丽,路赵硕,吴越,等.铜、铁、锌、铝通过Akt/GSK-3β信号通路诱导SH-SY5Y细胞的凋亡[J].实用老年医学,2017,31(8):723-727.
[15]卢丽,吴亮,陈珺,等.钠离子对成骨细胞的影响及其与上皮钠通道的关系[J].南方医科大学学报,2011,31(11):1871-1874.
[16]Florence Lefranc,Robert Kiss.The sodium pump alpha1 subunit as a potential target to combat apoptosis-resistant glioblastomas[J].Neoplasia,2008,10(3):198-206.
[17]徐忠伟,王凤梅,王聪聪,等.钠钾ATP酶抑制剂通过调节DNA损伤感应复合体Mre11/Rad50/Nbs1的表达诱导肝癌HepG2细胞周期阻滞[J].中国药理学通报,2016,32(3):323-327.
[18]鲍鹏,李国祥.硒抗肿瘤机理研究进展和展望[J].生物技术进展,2017,7(5):506-510.
[19]邵世和,孙丽媛.微量元素Se与肿瘤的关系[J].北华大学学报(自然科学版),2003(3):223-226.
[20]Marjory S Stewart,Julian E Spallholz,Kenneth H Neldner,et al.Selenium compounds have disparate abilities to impose oxidative stress and induce apoptosis[J].Free Radical Biology and Medicine,1999,26(1):42-48.
[21]李丽,古扎丽努尔·阿不力孜.微量元素与宫颈癌的研究进展[J].广东微量元素科学,2015,22(1):53-57.
[22]孟红旭,姚明江,刘建勋.人参皂苷Re对大鼠心室肌细胞钠、钾离子通道的影响[J].世界中医药,2013,8(10):1147-1149.
[23]Roderick H Llewelyn,Cook Simon J.Ca2+signalling checkpoints in cancer:remodelling Ca2+for cancer cell proliferation and survival[J].Nature Reviews Cancer,2008,8(5):361-375.