Magnetic Fe_3O_4@mTiO_2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins
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摘要
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe_3 O_4@TiO_2(Fe_3 O_4@mTiO_2) microsphere and a 5-aminoisophthalic acid(AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe_3 O_4@mTiO_2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe_3 O_4@mTiO_2-AIPA was successfully utilized to separate α-casein(a typical phosphoprotein) and bovine serum albumin(BSA, a typical non-phosphoprotein) from their mixtures(molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe_3 O_4@mTiO_2-AIPA for application in phosphoprotein enrichment.
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe_3 O_4@TiO_2(Fe_3 O_4@mTiO_2) microsphere and a 5-aminoisophthalic acid(AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe_3 O_4@mTiO_2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe_3 O_4@mTiO_2-AIPA was successfully utilized to separate α-casein(a typical phosphoprotein) and bovine serum albumin(BSA, a typical non-phosphoprotein) from their mixtures(molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe_3 O_4@mTiO_2-AIPA for application in phosphoprotein enrichment.
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe_3 O_4@TiO_2(Fe_3 O_4@mTiO_2) microsphere and a 5-aminoisophthalic acid(AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe_3 O_4@mTiO_2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe_3 O_4@mTiO_2-AIPA was successfully utilized to separate α-casein(a typical phosphoprotein) and bovine serum albumin(BSA, a typical non-phosphoprotein) from their mixtures(molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe_3 O_4@mTiO_2-AIPA for application in phosphoprotein enrichment.
引文
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[34]Xu H,Zhang Y,Niu X,et al.Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres[J].J.Wuhan Univ.Technol.,-Mater.Sci.Ed.,2013,28(6):1 231-1 235
[35]Li C,Younesi R,Cai Y,et al.Photocatalytic and Antibacterial Properties of Au-Decorated Fe3O4@mTiO2 Core-Shell Microspheres[J].Appl.Catal.,B,2014,156-157:314-322
[36]Song H,Ma X,Xiong F,et al.Preparation and Evaluation of Insulin-Loaded Nanoparticles based on Hydroxypropyl-β-Cyclodextrin Modifed Carboxymethyl Chitosan for Oral Delivery[J].J.Wuhan Univ.Technol.-Mater.Sci.Ed.,2016,31(6):1 394-1 400
[37]Jin WH,Dai J,Li SJ,et al.Human Plasma Proteome Analysis by Multidimensional Chromatography Prefractionation and Linear Ion Trap Mass Spectrometry Identification[J].J.Proteome Res.,2005,4(2):613-619
[38]Canas B,Pineiro C,Calvo E,et al.Trends in Sample Preparation for Classical and Second Generation Proteomics[J].J.Chromatogr.A,2007,1 153(1-2):235-258
[39]Wuhrer M,Deelder AM,Hokke CH.Protein Glycosylation Analysis by Liquid Chromatography-Mass Spectrometry[J].J.Chromatogr.B,2005,825(2):124-133
[2]Grimsrud PA,Swaney DL,Wenger CD,et al.Phosphoproteomics for the Masses[J].ACS Chem.Biol.,2010,5(1):105-119
[3]Graves JD,Krebs EG.Protein Phosphorylation and Signal Transduction[J].Pharmacol.Ther.,1999,82(2-3):111-121
[4]Gong CX,Singh TJ,Grundke-Igbal I,et al.Alzheimer’s Disease Abnormally PhosphorylatedτIs Dephosphorylated by Protein Phosphatase-2B(Calcineurin)[J].J.Neurochem.,1994,62(2):803-806
[5]Solari FA,Dell’Aica M,Sickmann A,et al.Why Phosphoproteomics Is Still a Challenge[J].Mol.BioSyst.,2015,11(6):1 487-1 493
[6]Engholm-Keller K,Larsen MR.Technologies and Challenges in LargeScale Phosphoproteomics[J].Proteomics,2013,13(6):910-931
[7]Hou J,Xie Z,Xue P,et al.Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix[J].J.Biomed.Biotechnol.,2010:759 690
[8]Xiong Z,Chen Y,Zhang L,et al.Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides[J].ACS Appl.Mater.Interfaces,2014,6(24):22 743-22 750
[9]Wu S,Lourette NM,Tolic N,et al.An Integrated Top-Down and Bottom-Up Strategy for Broadly Characterizing Protein Isoforms and Modifications[J].J.Proteome Res.,2009,8(3):1 347-1 357
[10]Chait BT.Mass Spectrometry:Bottom-Up or Top-Down?[J].Science,2006,314(5 796):65-66
[11]Delom F,Chevet E.Phosphoprotein Analysis:from Proteins to Proteomes[J].Proteome Sci.,2006,4:15
[12]Yates JR,Ruse CI,Nakorchevsky A.Proteomics by Mass Spectrometry:Approaches,Advances,and Applications[J].Annu.Rev.Biomed.Eng.,2009,11:49-79
[13]Han X,Wang Y,Aslanian A,et al.Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Top-Down Characterization of Pyrococcus Furiosus Proteins on a Proteome Scale[J].Anal.Chem.,2014,86(22):11 006-11 012
[14]Siuti N,Kelleher NL.Decoding Protein Modifications Using TopDown Mass Spectrometry[J].Nat Methods.,2007,4(10):817-821
[15]Waanders LF,Hanke S,Mann M.Top-Down Quantitation and Characterization of SILAC-Labeled Proteins[J].J.Am.Soc.Mass Spectrom.,2007,18(11):2 058-2 064
[16]Tran JC,Zamdborg L,Ahlf DR,et al.Mapping Intact Protein Isoforms in Discovery Mode Using Top-Down Proteomics[J].Nature,2011,480(7 376):254-258
[17]Schmidt SR,Schweikart F,Andersson ME.Current Methods for Phosphoprotein Isolation and Enrichment[J].J.Chromatogr.B,2007,849(1-2):154-162
[18]Hwang L,Ayaz-Guner S,Gregorich ZR,et al.Specific Enrichment of Phosphoproteins Using Functionalized Multivalent Nanoparticles[J].J.Am.Chem.Soc.,2015,137(7):2 432-2 243
[19]Liu H,Yang T,Dai J,et al.Hydrophilic Modification of Titania Nanomaterials as a Biofunctional Adsorbent for Selective Enrichment of Phosphopeptides[J].Analyst,2015,140(19):6 652-6 659
[20]Yan YH,Zhang XM,Deng CH.Designed Synthesis of Titania Nanoparticles Coated Hierarchially Ordered Macro/Mesoporous Silica for Selective Enrichment of Phosphopeptides[J].ACS Appl.Mater.Interfaces,2014,6(8):5 467-5 471
[21]Li Y,Xu X,Qi D,et al.Novel Fe3O4@TiO2 Core-Shell Microspheres for Selective Enrichment of Phosphopeptides in Phosphoproteome Analysis[J].J.Proteome Res.,2008,7(6):2 526-2 538
[22]Mann M,Ong SE,Gronborg M,et al.Analysis of Protein Phosphorylation Using Mass Spectrometry:Deciphering the Phosphoproteome[J].Trends Biotechnol.,2002,20(6):261-268
[23]Tang J,Yin P,Lu X,et al.Development of Mesoporous TiO2 Microspheres with High Specific Surface Area for Selective Enrichment of Phosphopeptides by Mass Spectrometric Analysis[J].J.Chromatogr.A,2010,1217(15):2 197-2 205
[24]Qing G,Wang X,Jiang L,et al.Saccharide-Sensitive Wettability Switching on a Smart Polymer Surface[J].Soft Matter.,2009,5(14):2 759-2 765
[25]Liu S,Kang J,Cao X,et al.Acylthiourea Derivatives as Colorimetric Sensors for Anions:Synthesis,Characterization and Spectral Behaviors[J].Spectrochim.Acta,Part A,2016,153:471-477
[26]Nishio T,Ayano E,Suzuki Y,et al.Separation of Phosphorylated Peptides Utilizing Dual pH-and Temperature-Responsive Chromatography[J].J.Chromatogr.A,2011,1218(15):2 079-2 084
[27]Lu L,Li W,Wang G,et al.Synthesis and Characterization of Biomimetic Fe3O4/Coke Magnetic Nanoparticles Composite Material[J].J.Wuhan Univ.Technol.,-Mater.Sci.Ed.,2016,31(2):254-259
[28]Ren Q,Chu H,Chen M,et al.Design and Fabrication of Superparamaganitic Hybrid Microspheres for Protein Immobilization[J].J.Wuhan Univ.Technol.-Mater.Sci.Ed.,2011,26(6):1 084-1 088
[29]Chen CT,Chen YC.Fe3O4/TiO2 Core/Shell Nanoparticles as Affinity Probes for the Analysis of Phosphopeptides Using TiO2 Surface-Assisted Laser Desorption/Ionization Mass Spectrometry[J].Anal.Chem.,2005,77(18):5 912-5 919
[30]Deng H,Li X,Peng Q,et al.Monodisperse Magnetic Single-Crystal Ferrite Microspheres[J].Angew.Chem.Int.Ed.,2005,44(18):2 782-2 785
[31]Wang P,Chen D,Tang FQ.Preparation of Titania-Coated Polystyrene Particles in Mixed Solvents by Ammonia Catalysis[J].Langmuir,2006,22(10):4 832-4 835
[32]Gelb LD,Gubbins KE.Characterization of Porous Glasses:Simulation Models,Adsorption Isotherms,and the Brunauer-Emmett-Teller Analysis Method[J].Langmuir,1998,14(8):2 097-2 111
[33]Ojeda ML,Esparza JM,Campero A,et al.On Comparing BJH and NLDFT Pore-Size Distributions Determined from N2 Sorption on SBA-15 Substrata[J].Phys.Chem.Chem.Phys.,2003,5(9):1 859-1 866
[34]Xu H,Zhang Y,Niu X,et al.Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres[J].J.Wuhan Univ.Technol.,-Mater.Sci.Ed.,2013,28(6):1 231-1 235
[35]Li C,Younesi R,Cai Y,et al.Photocatalytic and Antibacterial Properties of Au-Decorated Fe3O4@mTiO2 Core-Shell Microspheres[J].Appl.Catal.,B,2014,156-157:314-322
[36]Song H,Ma X,Xiong F,et al.Preparation and Evaluation of Insulin-Loaded Nanoparticles based on Hydroxypropyl-β-Cyclodextrin Modifed Carboxymethyl Chitosan for Oral Delivery[J].J.Wuhan Univ.Technol.-Mater.Sci.Ed.,2016,31(6):1 394-1 400
[37]Jin WH,Dai J,Li SJ,et al.Human Plasma Proteome Analysis by Multidimensional Chromatography Prefractionation and Linear Ion Trap Mass Spectrometry Identification[J].J.Proteome Res.,2005,4(2):613-619
[38]Canas B,Pineiro C,Calvo E,et al.Trends in Sample Preparation for Classical and Second Generation Proteomics[J].J.Chromatogr.A,2007,1 153(1-2):235-258
[39]Wuhrer M,Deelder AM,Hokke CH.Protein Glycosylation Analysis by Liquid Chromatography-Mass Spectrometry[J].J.Chromatogr.B,2005,825(2):124-133