不同温度下喷施油菜素内酯对香椿幼苗的影响
|
摘要
为了探究外源油菜素内酯(BR)对不同温度(8、28、38℃)下香椿幼苗生长的调节作用,以盆栽5月龄香椿幼苗为试材,以喷施清水为对照,研究外源油菜素内酯对不同温度下香椿幼苗生物量、叶绿素含量、光合特性、叶绿素荧光特性的影响。结果表明;外源BR处理可提高不同温度下香椿幼苗叶绿素的含量,显著提高香椿幼苗生物量和干物质积累量,使香椿幼苗叶片组织加厚,叶片相对含水率(RWC)提升,并且提高了香椿幼苗的净光合速率(P_n)、气孔导度(Gs)、最大光化学效率(F_v/F_m)、实际光化学效率(ΦPSⅡ)和光化学猝灭系数(qP),降低了胞间CO_2浓度(C_i)、蒸腾速率(T_r)、初始荧光(F_o)、非光化学猝灭系数(qN)。综上所述:喷施油菜素内酯可以促进适温下(28℃)香椿幼苗植株的生长,提高香椿幼苗在温度胁迫下的缓解作用和适应能力。
To investigate effects of spraying Brassinolide on the biomass, chlorophyll content, photosynthetic characteristics and chlorophyll fluorescence of seedlings of Toona sinensis in different Temperatures(8℃, 28℃, 38℃), 5-month-old Toona sinensis seedlings were taken as the experimental material and the seedlings were sprayed with distilled water as the control. The results revealed that BR application increased the chlorophyll contents in different temperatures, significantly raised the seeding biomass and dry matter accumulation, thickened the leaf tissue and enhanced leaf relative water content of seedlings of Toona sinensis. With the BR treatments, the net photosynthetic rate, stomatal conductance, maximum photochemical efficiency, actual photochemical efficiency and photochemical quenching coefficient were increased. The rate of intercellular CO_2 concentration, transpiration rate, initial fluorescence and non-photochemical quenching coefficient were decreased. To sum up, the spraying Brassinolide treatments can promote the growth of seedlings of Toona sinensis in suitable temperature(28℃) and increase the mitigation and adaptation of seedlings of Toona sinensis to different temperature stress.
To investigate effects of spraying Brassinolide on the biomass, chlorophyll content, photosynthetic characteristics and chlorophyll fluorescence of seedlings of Toona sinensis in different Temperatures(8℃, 28℃, 38℃), 5-month-old Toona sinensis seedlings were taken as the experimental material and the seedlings were sprayed with distilled water as the control. The results revealed that BR application increased the chlorophyll contents in different temperatures, significantly raised the seeding biomass and dry matter accumulation, thickened the leaf tissue and enhanced leaf relative water content of seedlings of Toona sinensis. With the BR treatments, the net photosynthetic rate, stomatal conductance, maximum photochemical efficiency, actual photochemical efficiency and photochemical quenching coefficient were increased. The rate of intercellular CO_2 concentration, transpiration rate, initial fluorescence and non-photochemical quenching coefficient were decreased. To sum up, the spraying Brassinolide treatments can promote the growth of seedlings of Toona sinensis in suitable temperature(28℃) and increase the mitigation and adaptation of seedlings of Toona sinensis to different temperature stress.
引文
[1]祁承经,汤庚国.树木学(南方本)[M].北京:中国林业出版社,2005:381-382.
[2]李辉,左钦月,涂升斌.油菜素内酯生物合成和代谢研究进展[J].植物生理学报,2015,51(11):1787-1798.
[3]CLOUSE S D,SASSE J M.Brassinosteroids:essential regulators of plant growth and development[J].Annu.Rev.Plant Physiol.Plant.Mol.Biol.,1998,49:47-51.
[4]杨慧杰,原向阳,郭平毅,等.油菜素内酯对阔世玛胁迫下谷子叶片光合荧光特性及糖代谢的影响[J].中国农业科学,2017,50(13):2508-2518.
[5]王舒甜,张金池,张亮.油菜素内酯对盐胁迫下香樟叶片光合色素以及叶绿素荧光的影响[J].浙江大学学报(农业与生命科学版),2017,43(1):45-53.
[6]吴杨,高慧纯,张必弦,等.2,4-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响[J].中国农业科学,2017,50(5):811-821.
[7]袁凌云,汪承刚,黄兴学,等.油菜素内酯对Ca(NO3)2胁迫下黄瓜叶绿体AsA-GSH循环及叶黄素稳态的影响[J].园艺学报,2017,44(5):881-890.
[8]李泽,谭晓风,卢锟,等.供镁水平对油桐幼苗生长及光合特性的影响[J].生态学杂志,2015,34(9):2440-2447.
[9]尚庆茂,宋士清,张志刚,等.外源BR诱导黄瓜(Cucumis sativus L.)幼苗的抗盐性[J].中国农业科学,2006,39(9):1872-1877.
[10]DHAUBHADEL S,CHAUDHARY S,DOBINSON K F,et al.Treat-ment with 24-epibrassinolide,a brassinosteroid,increases the basic thermotolerance of Brassica napus and tomato seedlings[J].Plant Mol.Biol.,1999,40:333-342.
[11]吴晓丽,罗立津,黄丽岚,等.水杨酸和油菜素内酯对花椰菜幼苗生长及抗旱性的影响[J].干旱地区农业研究,2011,29(2):168-172.
[12]王传凯,郭淼.油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响[J].南方农业学报,2017,48(7):1173-1177.
[13]谢云灿,何孝磊,杜鹏,等.外源油菜素内酯对高温胁迫下大豆光合特性及产量品质的影响[J].大豆科学,2017,36(2):237-243.
[14]赵爽,刘洋,卢铁兵,等.不同时期‘绿岭’核桃叶片光合和荧光特性的动态变化[J].经济林研究,2017,35(4):1-8.
[15]刘雪,彭冶,范俊俊,等.淹水胁迫对湖北海棠生长和生理特性的影响[J].经济林研究,2018,36(1):35-42.
[16]徐晓昀,郁继华,颉建明,等.2,4-表油菜素内酯对亚适温弱光下黄瓜幼苗光合特性和抗氧化系统的影响[J].核农学报,2017,31(5):979-986.
[17]杨猛,魏玲,胡萌,等.低温胁迫对玉米幼苗光合特性的影响[J].东北农业大学学报,2012,43(1):66-71.
[18]GEIGER D R,SERVAITES J C,FUCHS M A,et al.Role of starch in carbon translocation and partitioning at the plant level[J].Functional Plant Biology,2000,27(6):571-582.
[19]泰兹,奇格尔.植物生理学[M].5版.宋纯鹏,王学路,周云,等译.北京:科学出版社,2015.
[20]FARQUHAR G D,SHARKEY T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33:317-345.
[21]张永平,陈幼源,杨少军.高温胁迫下2,4-表油菜素内酯对甜瓜幼苗生理及光合特性的影响[J].植物生理学报,2012,48(7):683-688.
[22]丁坤元,刘艳红.氮肥和连续夜间低温对珙桐幼苗叶片光合特性的影响[J].东北林业大学学报,2015,43(3):56-61.
[23]GENTY B,BRLANTAIS J M,BAKER N R.The relationship between quantum yield of photosynthetic electron transportand quenching of chlorophyll fluorescence[J].Biochim Biophys Acta Gen Subiect,1989,990(1):87-92.
[24]张永平,范红伟,杨少军,等.表油菜素内酯对高温胁迫下甜瓜幼苗叶绿素荧光特性的影响[J].上海农业学报,2012,28(3):17-21.
[25]吕芳德,徐德聪,侯红波,等.5种红山茶叶绿素荧光特性的比较研究[J].经济林研究,2003,21(4):4-7.
[26]张睿佳,李瑛,虞秀明,等.高温胁迫与外源油菜素内酯对‘巨峰’葡萄叶片光合生理和果实品质的影响[J].果树学报,2015,32(4):590-596.
[27]吴思政,梁文斌,聂东伶,等.高温胁迫对不同蓝莓品种光合作用的影响[J].中南林业科技大学学报,2017,37(11):1-8.
[2]李辉,左钦月,涂升斌.油菜素内酯生物合成和代谢研究进展[J].植物生理学报,2015,51(11):1787-1798.
[3]CLOUSE S D,SASSE J M.Brassinosteroids:essential regulators of plant growth and development[J].Annu.Rev.Plant Physiol.Plant.Mol.Biol.,1998,49:47-51.
[4]杨慧杰,原向阳,郭平毅,等.油菜素内酯对阔世玛胁迫下谷子叶片光合荧光特性及糖代谢的影响[J].中国农业科学,2017,50(13):2508-2518.
[5]王舒甜,张金池,张亮.油菜素内酯对盐胁迫下香樟叶片光合色素以及叶绿素荧光的影响[J].浙江大学学报(农业与生命科学版),2017,43(1):45-53.
[6]吴杨,高慧纯,张必弦,等.2,4-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响[J].中国农业科学,2017,50(5):811-821.
[7]袁凌云,汪承刚,黄兴学,等.油菜素内酯对Ca(NO3)2胁迫下黄瓜叶绿体AsA-GSH循环及叶黄素稳态的影响[J].园艺学报,2017,44(5):881-890.
[8]李泽,谭晓风,卢锟,等.供镁水平对油桐幼苗生长及光合特性的影响[J].生态学杂志,2015,34(9):2440-2447.
[9]尚庆茂,宋士清,张志刚,等.外源BR诱导黄瓜(Cucumis sativus L.)幼苗的抗盐性[J].中国农业科学,2006,39(9):1872-1877.
[10]DHAUBHADEL S,CHAUDHARY S,DOBINSON K F,et al.Treat-ment with 24-epibrassinolide,a brassinosteroid,increases the basic thermotolerance of Brassica napus and tomato seedlings[J].Plant Mol.Biol.,1999,40:333-342.
[11]吴晓丽,罗立津,黄丽岚,等.水杨酸和油菜素内酯对花椰菜幼苗生长及抗旱性的影响[J].干旱地区农业研究,2011,29(2):168-172.
[12]王传凯,郭淼.油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响[J].南方农业学报,2017,48(7):1173-1177.
[13]谢云灿,何孝磊,杜鹏,等.外源油菜素内酯对高温胁迫下大豆光合特性及产量品质的影响[J].大豆科学,2017,36(2):237-243.
[14]赵爽,刘洋,卢铁兵,等.不同时期‘绿岭’核桃叶片光合和荧光特性的动态变化[J].经济林研究,2017,35(4):1-8.
[15]刘雪,彭冶,范俊俊,等.淹水胁迫对湖北海棠生长和生理特性的影响[J].经济林研究,2018,36(1):35-42.
[16]徐晓昀,郁继华,颉建明,等.2,4-表油菜素内酯对亚适温弱光下黄瓜幼苗光合特性和抗氧化系统的影响[J].核农学报,2017,31(5):979-986.
[17]杨猛,魏玲,胡萌,等.低温胁迫对玉米幼苗光合特性的影响[J].东北农业大学学报,2012,43(1):66-71.
[18]GEIGER D R,SERVAITES J C,FUCHS M A,et al.Role of starch in carbon translocation and partitioning at the plant level[J].Functional Plant Biology,2000,27(6):571-582.
[19]泰兹,奇格尔.植物生理学[M].5版.宋纯鹏,王学路,周云,等译.北京:科学出版社,2015.
[20]FARQUHAR G D,SHARKEY T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33:317-345.
[21]张永平,陈幼源,杨少军.高温胁迫下2,4-表油菜素内酯对甜瓜幼苗生理及光合特性的影响[J].植物生理学报,2012,48(7):683-688.
[22]丁坤元,刘艳红.氮肥和连续夜间低温对珙桐幼苗叶片光合特性的影响[J].东北林业大学学报,2015,43(3):56-61.
[23]GENTY B,BRLANTAIS J M,BAKER N R.The relationship between quantum yield of photosynthetic electron transportand quenching of chlorophyll fluorescence[J].Biochim Biophys Acta Gen Subiect,1989,990(1):87-92.
[24]张永平,范红伟,杨少军,等.表油菜素内酯对高温胁迫下甜瓜幼苗叶绿素荧光特性的影响[J].上海农业学报,2012,28(3):17-21.
[25]吕芳德,徐德聪,侯红波,等.5种红山茶叶绿素荧光特性的比较研究[J].经济林研究,2003,21(4):4-7.
[26]张睿佳,李瑛,虞秀明,等.高温胁迫与外源油菜素内酯对‘巨峰’葡萄叶片光合生理和果实品质的影响[J].果树学报,2015,32(4):590-596.
[27]吴思政,梁文斌,聂东伶,等.高温胁迫对不同蓝莓品种光合作用的影响[J].中南林业科技大学学报,2017,37(11):1-8.