外源GA_3和ABA处理条件下‘兰州百合’鳞茎内源激素的变化
|
摘要
【目的】研究外源激素赤霉素(GA_3)与脱落酸(ABA)处理对休眠解除过程中兰州百合鳞茎内源激素的变化规律.【方法】以百合品种‘兰百1号’为试验材料,采取外源GA_3与ABA浸泡处理百合种球12h,测定在2℃贮藏条件下,兰州百合鳞茎内源激素的含量.【结果】在打破休眠过程中,外源GA_3处理的百合鳞茎内源GA_3、ABA、IAA与ZR含量及比值变化的速率加快、提前达到最值,且百合鳞茎萌发时间提前15d;ABA处理结果正好相反,变化速率变缓、延缓到达最值,且百合鳞茎萌发时间推迟20d.【结论】根据相关分析结果初步判定,‘兰州百合’打破休眠主要受内源GA_3和ABA的调控,可能还与两者的比值有关.外源GA_3会促进‘兰州百合’休眠的解除,外源ABA则会抑制休眠解除,二者均可通过使与休眠相关的内源激素变化来调控休眠进程.
【Objective】To study the changes of endogenous hormones in lily bulbs during the dormancy release by exogenous hormones GA_3 and abscisic acid(ABA).【Method】With Liliumvariety ‘Lanbai No.1' as experimental material,exogenous GA_3 and ABA were used to treat the lily bulbs,and the changes of endogenous hormone content in the bulb during dormancy release were determined.【Result】The GA_3 treatment increased the content of GA_3,ABA,IAA and ZR and the ratio of GA_3,and reached the peak value in advance,and the germination time of bulb rose 15 days ahead of time.The rate of change slowed down,the delay reached the maximum value,and the bulb germination time was postponed for 20 days.【Conclusion】Based on the results of correlation analysis,it is suggested that the dormancy release of lily bulbs is mainly regulated by endogenous GA_3 and ABA.Exogenous GA_3 promotes the release of dormancy of lily and exogenous ABA inhibits it.Both of them can regulate the dormancy process by changing the endogenous hormones associated with dormancy.
【Objective】To study the changes of endogenous hormones in lily bulbs during the dormancy release by exogenous hormones GA_3 and abscisic acid(ABA).【Method】With Liliumvariety ‘Lanbai No.1' as experimental material,exogenous GA_3 and ABA were used to treat the lily bulbs,and the changes of endogenous hormone content in the bulb during dormancy release were determined.【Result】The GA_3 treatment increased the content of GA_3,ABA,IAA and ZR and the ratio of GA_3,and reached the peak value in advance,and the germination time of bulb rose 15 days ahead of time.The rate of change slowed down,the delay reached the maximum value,and the bulb germination time was postponed for 20 days.【Conclusion】Based on the results of correlation analysis,it is suggested that the dormancy release of lily bulbs is mainly regulated by endogenous GA_3 and ABA.Exogenous GA_3 promotes the release of dormancy of lily and exogenous ABA inhibits it.Both of them can regulate the dormancy process by changing the endogenous hormones associated with dormancy.
引文
[1]陈艳华,郭俊琴,张旭东.基于GIS的兰州百合适生种植气候区划[J].干旱气象,2014,32(1):157-161.
[2] Juntilla O.To be or not to be dormant:Some comments on the new dormancy nomenclature[J].Hort Science,1988,23(5):805-806.
[3]孙红梅,李天来,李云飞.百合鳞茎发育过程中内源激素变化初探[J].华中农业大学学报(自然科学版),2004(z2):277-281.
[4]李翊华,安丽萍,李彬,等.低温对百合鳞茎内源激素含量变化的影响[J].中国沙漠,2011,31(5):1208-1214.
[5]李亚莉,侯栋,马真胜,等.兰州百合优质栽培技术[J].中国蔬菜,2016(10):89-91.
[6]孙红梅,李天来,李云飞.兰州百合鳞茎发育及低温解除休眠过程中内源激素的变化[J].植物研究,2006(5):570-576.
[7] Endo Y,Arisaka E,Niimi Y.Effects of chilling and GA3treatments on breaking dormancy in Lilium rubellabaker bulblets cultured in vitro[J].Journal of the Japanese Society for Horticultural Science,1988,57(2):250-257.
[8]杨卫兵,王振林,尹燕枰,等.外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响[J].中国农业科学,2011,44(13):2673-2682.
[9]金鑫,李彬,牛丽涓,等.低温和赤霉素对百合鳞茎休眠解除的影响[J].中国沙漠,2016,36(5):1315-1322.
[10]任秀霞,王顺利,薛璟祺,等.GA和ABA信号转导关键基因在不同温度处理‘凤丹’牡丹种子中的表达分析[J].园艺学报,2017,44(1):80-88.
[11]赵海涛,刘春,明军,等.ABA对‘西伯利亚’百合试管鳞茎发育及休眠的影响[J].园艺学报,2010,37(3):428-434.
[12]黄彦玮,师桂英,李谋强,等.外源GA3、ABA对低温冷藏兰州百合种球萌发的促抑效应及种球碳水化合物变化研究[J].甘肃农大学报,2016,51(1):55-61.
[13]冯晶红,张秀娟,贾桂霞.低温对东方百合试管鳞茎解除休眠及生长的影响[J].植物生理学报,2013,49(12):1427-1432.
[14]李云飞,孙红梅,李天来.低温解除休眠过程中兰州百合中部鳞片物质变化的生理机制[J].中国农业科学,2005,38(2):376-382.
[15]黄永旺,游向阳,方少忠.低温层积与外源激素对百合试管小鳞茎活力的影响[J].福建农业科技,2016(9):26-29.
[16] Choi S T,Jung W Y.Ahn H G,et al.Effects of duration ofcold treatment and planting depth on growth and flowering of Liliumspp.[J].Journal of the Korean Society for Horticultural Science,1998,39(6):765-770.
[17]刘悦善,李成,王东霞,等.加工型马铃薯品种微型薯种薯贮藏期内源激素含量、阈值和休眠期相关性分析[J].中国农业科学,2015,48(2):262-269.
[18]段成国,李宪利,高东升,等.内源ABA和GA3对欧洲甜樱桃花芽自然休眠的调控[J].园艺学报,2004,31(2):149-154.
[19] Shin K S,ChakrabartyD,Paek K Y.Sproutingrate,change of carbohydrate content and related enzymes during cold treatment of lily bublets regenerated in vitro[J].Scientia Horticulturae,2002,96:195-204.
[20] Roh S M.Dormancy and maturity in the bulblet of Lilium lancifolium:the influence of growth regulator treatment on the grow and flowering response[J].Journal of the Korean Society for Horticultural Science,1982,23(1):59-63.
[21] Xiaohua Liu,Qing Wang,JiahuiGu,et al.Vernalization of Oriental hybrid lily Sorbonne':changesin physiology metabolic activity and molecular mechanism[J].Moleular Biology Reports,2014,41:6619-6634.
[22]陈鸿,李智辉,李天来,等.新铁炮百合花芽分化及发育过程中内源多胺及激素含量变化的研究[J].沈阳农业大学学报,2010,41(3):284-288.
[23]黄永旺,游向阳,方少忠.低温层积与外源激素对百合试管小鳞茎活力的影响[J].福建农业科技,2016(9):26-29.
[24]夏宜平,黄春辉,何桂芳,等.东方百合鳞茎冷藏解除休眠的养分代谢和酶活性变化[J].园艺学报,2006,33(3):571-576.
[2] Juntilla O.To be or not to be dormant:Some comments on the new dormancy nomenclature[J].Hort Science,1988,23(5):805-806.
[3]孙红梅,李天来,李云飞.百合鳞茎发育过程中内源激素变化初探[J].华中农业大学学报(自然科学版),2004(z2):277-281.
[4]李翊华,安丽萍,李彬,等.低温对百合鳞茎内源激素含量变化的影响[J].中国沙漠,2011,31(5):1208-1214.
[5]李亚莉,侯栋,马真胜,等.兰州百合优质栽培技术[J].中国蔬菜,2016(10):89-91.
[6]孙红梅,李天来,李云飞.兰州百合鳞茎发育及低温解除休眠过程中内源激素的变化[J].植物研究,2006(5):570-576.
[7] Endo Y,Arisaka E,Niimi Y.Effects of chilling and GA3treatments on breaking dormancy in Lilium rubellabaker bulblets cultured in vitro[J].Journal of the Japanese Society for Horticultural Science,1988,57(2):250-257.
[8]杨卫兵,王振林,尹燕枰,等.外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响[J].中国农业科学,2011,44(13):2673-2682.
[9]金鑫,李彬,牛丽涓,等.低温和赤霉素对百合鳞茎休眠解除的影响[J].中国沙漠,2016,36(5):1315-1322.
[10]任秀霞,王顺利,薛璟祺,等.GA和ABA信号转导关键基因在不同温度处理‘凤丹’牡丹种子中的表达分析[J].园艺学报,2017,44(1):80-88.
[11]赵海涛,刘春,明军,等.ABA对‘西伯利亚’百合试管鳞茎发育及休眠的影响[J].园艺学报,2010,37(3):428-434.
[12]黄彦玮,师桂英,李谋强,等.外源GA3、ABA对低温冷藏兰州百合种球萌发的促抑效应及种球碳水化合物变化研究[J].甘肃农大学报,2016,51(1):55-61.
[13]冯晶红,张秀娟,贾桂霞.低温对东方百合试管鳞茎解除休眠及生长的影响[J].植物生理学报,2013,49(12):1427-1432.
[14]李云飞,孙红梅,李天来.低温解除休眠过程中兰州百合中部鳞片物质变化的生理机制[J].中国农业科学,2005,38(2):376-382.
[15]黄永旺,游向阳,方少忠.低温层积与外源激素对百合试管小鳞茎活力的影响[J].福建农业科技,2016(9):26-29.
[16] Choi S T,Jung W Y.Ahn H G,et al.Effects of duration ofcold treatment and planting depth on growth and flowering of Liliumspp.[J].Journal of the Korean Society for Horticultural Science,1998,39(6):765-770.
[17]刘悦善,李成,王东霞,等.加工型马铃薯品种微型薯种薯贮藏期内源激素含量、阈值和休眠期相关性分析[J].中国农业科学,2015,48(2):262-269.
[18]段成国,李宪利,高东升,等.内源ABA和GA3对欧洲甜樱桃花芽自然休眠的调控[J].园艺学报,2004,31(2):149-154.
[19] Shin K S,ChakrabartyD,Paek K Y.Sproutingrate,change of carbohydrate content and related enzymes during cold treatment of lily bublets regenerated in vitro[J].Scientia Horticulturae,2002,96:195-204.
[20] Roh S M.Dormancy and maturity in the bulblet of Lilium lancifolium:the influence of growth regulator treatment on the grow and flowering response[J].Journal of the Korean Society for Horticultural Science,1982,23(1):59-63.
[21] Xiaohua Liu,Qing Wang,JiahuiGu,et al.Vernalization of Oriental hybrid lily Sorbonne':changesin physiology metabolic activity and molecular mechanism[J].Moleular Biology Reports,2014,41:6619-6634.
[22]陈鸿,李智辉,李天来,等.新铁炮百合花芽分化及发育过程中内源多胺及激素含量变化的研究[J].沈阳农业大学学报,2010,41(3):284-288.
[23]黄永旺,游向阳,方少忠.低温层积与外源激素对百合试管小鳞茎活力的影响[J].福建农业科技,2016(9):26-29.
[24]夏宜平,黄春辉,何桂芳,等.东方百合鳞茎冷藏解除休眠的养分代谢和酶活性变化[J].园艺学报,2006,33(3):571-576.