摘要
目的考察鸡血藤组方提取物在体外对肿瘤细胞增殖作用的影响及其机制。方法分别培养人肝癌HepG2细胞、肺癌A549细胞、鼻咽癌CNE-2Z细胞、骨肉瘤143B细胞、急性早幼粒细胞白血病HL-60细胞,加入0.0044~17.00 mg/mL不同浓度的鸡血藤组方提取物,培养72 h后CCK-8法测定细胞活力,计算鸡血藤组方提取物对各细胞株的抑制率及IC50值,Hoechst33258染色法观察HepG2细胞核的形态学变化。结果鸡血藤组方提取物干预HepG2、A549、CNE-2Z、143B、HL-60细胞72 h的IC50值分别为0.54、10.04、1.59、0.86、3.37 mg/mL。Hoechst33258染色显示鸡血藤组方提取物作用48 h后的HepG2细胞凋亡数明显增加。结论鸡血藤组方提取物对上述五个肿瘤细胞株的增殖均产生抑制作用,其中对肝癌HepG2细胞和骨肉瘤143B细胞的增殖抑制较强,并对HepG2细胞产生诱导凋亡作用。
Objective To explore the antitumor effects of the Millettia formulation extract in vitro and its mechanism. Methods HepG2、A549、CNE-2Z、143 B and HL-60 cells were cultured and seeded in 96-well plates at the appropriate cell density, CCK-8 assay was used to investigate the effects of Millettia formulation on the tumor cells. The anti-proliferative rate and IC50 value of different concentrations of Millettia formulation extract on each cell line were calculated. Hoechst33258 staining was used to observe the morphological changes of HepG2 cells. Results The Millettia formulation extract showed a certain inhibition on proliferation of HepG2, A549, CNE-2Z, 143 B and HL-60 cells with IC50 values of 0.54, 10.04, 1.59, 0.86 and 3.37 mg/mL, respectively. Hoechst 33258 staining showed that HepG2 cell apoptosis increased significantly after intervention 48 hours by Millettia formulation extract. Conclusion The proliferation of the five tumor cell lines were inhibited by Millettia formulation extract, especially HepG2 cells and 143 B cells showed good inhibitory effect, which can induce apoptosis in HepG2 cells.
引文
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