鸡血藤组方提取物的抗肿瘤作用及其机制初探
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摘要
目的考察鸡血藤组方提取物在体外对肿瘤细胞增殖作用的影响及其机制。方法分别培养人肝癌HepG2细胞、肺癌A549细胞、鼻咽癌CNE-2Z细胞、骨肉瘤143B细胞、急性早幼粒细胞白血病HL-60细胞,加入0.0044~17.00 mg/mL不同浓度的鸡血藤组方提取物,培养72 h后CCK-8法测定细胞活力,计算鸡血藤组方提取物对各细胞株的抑制率及IC50值,Hoechst33258染色法观察HepG2细胞核的形态学变化。结果鸡血藤组方提取物干预HepG2、A549、CNE-2Z、143B、HL-60细胞72 h的IC50值分别为0.54、10.04、1.59、0.86、3.37 mg/mL。Hoechst33258染色显示鸡血藤组方提取物作用48 h后的HepG2细胞凋亡数明显增加。结论鸡血藤组方提取物对上述五个肿瘤细胞株的增殖均产生抑制作用,其中对肝癌HepG2细胞和骨肉瘤143B细胞的增殖抑制较强,并对HepG2细胞产生诱导凋亡作用。
Objective To explore the antitumor effects of the Millettia formulation extract in vitro and its mechanism. Methods HepG2、A549、CNE-2Z、143 B and HL-60 cells were cultured and seeded in 96-well plates at the appropriate cell density, CCK-8 assay was used to investigate the effects of Millettia formulation on the tumor cells. The anti-proliferative rate and IC50 value of different concentrations of Millettia formulation extract on each cell line were calculated. Hoechst33258 staining was used to observe the morphological changes of HepG2 cells. Results The Millettia formulation extract showed a certain inhibition on proliferation of HepG2, A549, CNE-2Z, 143 B and HL-60 cells with IC50 values of 0.54, 10.04, 1.59, 0.86 and 3.37 mg/mL, respectively. Hoechst 33258 staining showed that HepG2 cell apoptosis increased significantly after intervention 48 hours by Millettia formulation extract. Conclusion The proliferation of the five tumor cell lines were inhibited by Millettia formulation extract, especially HepG2 cells and 143 B cells showed good inhibitory effect, which can induce apoptosis in HepG2 cells.
Objective To explore the antitumor effects of the Millettia formulation extract in vitro and its mechanism. Methods HepG2、A549、CNE-2Z、143 B and HL-60 cells were cultured and seeded in 96-well plates at the appropriate cell density, CCK-8 assay was used to investigate the effects of Millettia formulation on the tumor cells. The anti-proliferative rate and IC50 value of different concentrations of Millettia formulation extract on each cell line were calculated. Hoechst33258 staining was used to observe the morphological changes of HepG2 cells. Results The Millettia formulation extract showed a certain inhibition on proliferation of HepG2, A549, CNE-2Z, 143 B and HL-60 cells with IC50 values of 0.54, 10.04, 1.59, 0.86 and 3.37 mg/mL, respectively. Hoechst 33258 staining showed that HepG2 cell apoptosis increased significantly after intervention 48 hours by Millettia formulation extract. Conclusion The proliferation of the five tumor cell lines were inhibited by Millettia formulation extract, especially HepG2 cells and 143 B cells showed good inhibitory effect, which can induce apoptosis in HepG2 cells.
引文
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[10]靳宝辉,隋华,李琦.扶正中药逆转肿瘤多药耐药的研究进展[J].中华中医药杂志,2013,28(8):2367-2370.
[11]李东梅,蒋晓松,汤力昌,等.扶正解毒法配合新辅助化疗治疗乳腺癌33例临床研究[J].世界中医药,2010,5(6):394-396.
[2]Ma W,Zhang D,Zheng L,et al.Potential roles of Centipede Scolopendra extracts as a strategy against EGFR-dependent cancers[J].Am J Transl Res,2015,7(1):39-52.
[3]韩莉,周永芹,韩钰.蜈蚣提取物诱导宫颈癌Hela细胞凋亡及其机制的研究[J].时珍国医国药,2007,18(9):2109-2111.
[4]潘玉真,殷东风,邢玉庆,等.柴胡龙牡汤对小鼠Lewis肺癌细胞凋亡的影响及其机制的实验研究[J].中华中医药学刊,2014,32(10):2419-2422.
[5]Chow LW,Loo WT,Sham JS,et al.Radix bupleuri containing compound(KY88 liver-livo)induces apoptosis and production of interleukin-4 and tumor necrosis factor-alpha in liver cancer cells in vitro[J].Am J Chin Med.2004,32(2):185-193.
[6]武峰,秦志丰,李勇进,等.半夏化学成分抗肿瘤研究进展[J].中华中医药学刊,2013,31(2):270-272.
[7]Li N,Dong G,Wang S,et al.Pinellia pedatisecta agglutinin-based lectin blot analysis distinguishes between glycosylation patterns in various cancer cell lines[J].Oncol Lett,2014,8(2):837-840.
[8]董秀,韩兆峰,王晓波,等.厚朴酚对人小细胞肺癌H446细胞凋亡的影响[J].中国医药指南,2011,9(8):200-201.
[9]喻明,曹琦琛,苏玉茜,等.基于中医传承辅助系统的中医治疗肿瘤方剂组方分析及新方发现[J].中国中药杂志,2015,40(15):3110-3114.
[10]靳宝辉,隋华,李琦.扶正中药逆转肿瘤多药耐药的研究进展[J].中华中医药杂志,2013,28(8):2367-2370.
[11]李东梅,蒋晓松,汤力昌,等.扶正解毒法配合新辅助化疗治疗乳腺癌33例临床研究[J].世界中医药,2010,5(6):394-396.