染料木素-锌配合物的合成及抗癌活性研究
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摘要
目的研究染料木素-锌配合物的合成,并比较染料木素与锌金属离子配位前后的抗癌活性变化。方法以天然活性成分染料木素为配体与锌金属离子配位,合成染料木素-锌配合物。利用红外光谱、紫外光谱、核磁共振等方法对配合物进行表征。利用MTT法比较染料木素与锌金属离子配位前后的抗癌活性变化。结果合成得到的染料木素-锌配合物为淡黄色粉末,分子式为Zn(C15H9O5)2·2H2O,分子量为651。MTT试验结果表明,染料木素-锌配合物对MCF-7和H460细胞增殖抑制的效果明显高于其配体染料木素。结论染料木素-锌配合物的合成方法简便,其抗癌活性高于其配体染料木素。
Objective To study the synthesis of the genistein-zinc complexes and compare the change of anticancer activity before and after coordination between genistein and zinc metal ions. Methods Genistein-zinc complexes is prepared by the combination of nature compound genistein and zinc metal ions. The IR spectra, UV spectra, 1 HNMR are used to characterized the structure of genistein-zinc complexes. The MTT assay is used to compared the anticancer activities before and after coordination between genistein and zinc metal ions. Results The results show that the genistein-zinc complexes have been gotten, light yellow solid, whose molecular formula is Zn(C15 H9 O5)2·2 H2 O and molecular weidght(m/z) is 651. The MTT results are showed that the anticancer activity of genistein-zinc complexes on MCF-7 cells and H460 cells are obviously higher than its ligand genistein. Conclusion The synthesis method of genistein-zinc complexes is simple and its anticancer activity is higher than its ligand genistein.
Objective To study the synthesis of the genistein-zinc complexes and compare the change of anticancer activity before and after coordination between genistein and zinc metal ions. Methods Genistein-zinc complexes is prepared by the combination of nature compound genistein and zinc metal ions. The IR spectra, UV spectra, 1 HNMR are used to characterized the structure of genistein-zinc complexes. The MTT assay is used to compared the anticancer activities before and after coordination between genistein and zinc metal ions. Results The results show that the genistein-zinc complexes have been gotten, light yellow solid, whose molecular formula is Zn(C15 H9 O5)2·2 H2 O and molecular weidght(m/z) is 651. The MTT results are showed that the anticancer activity of genistein-zinc complexes on MCF-7 cells and H460 cells are obviously higher than its ligand genistein. Conclusion The synthesis method of genistein-zinc complexes is simple and its anticancer activity is higher than its ligand genistein.
引文
[1]周发阳,徐翊雯,孙宇,等.中药配位化学研究进展[J].中国现代中药,2015, 17(5):502-508.
[2]蒋伟.金莲花中荭草苷锌配合物合成、表征及其生物活性初步研究[D].张家口:河北北方学院, 2013.
[3]杨学娟,陈钧,陈华,等.发酵豆粕提取物中染料木素的分离纯化及结构鉴定[J].天然产物研究与开发, 2012, 24(S1):8-10.
[4]王建华.大豆异黄酮研究进展[J].现代中药研究与实践, 2013, 27(1):85-88.
[5]陈莉敏,刘洋,李光文,等.姜黄素金属配合物的合成、表征和抗肿瘤活性研究[J].中国新药杂志, 2008(19):1676-1678.
[6]吴明亮.桑色素锌(Ⅱ)、镉(Ⅱ)、钴(Ⅱ)配合物与DNA的作用机理及DNA电化学传感器的研究[D].青岛:青岛科技大学, 2009.
[7]钱俊臻,王伯初,谭君,等.黄酮类化合物的金属配合物及其药理作用[J].中国药理学通报, 2012, 28(8):1058-1062.
[8]周晶,王进义,龚国权,等.槲皮素合铜(Ⅱ)配合物与DNA相互作用的研究[J].兰州大学学报, 2000(6):131-133.
[2]蒋伟.金莲花中荭草苷锌配合物合成、表征及其生物活性初步研究[D].张家口:河北北方学院, 2013.
[3]杨学娟,陈钧,陈华,等.发酵豆粕提取物中染料木素的分离纯化及结构鉴定[J].天然产物研究与开发, 2012, 24(S1):8-10.
[4]王建华.大豆异黄酮研究进展[J].现代中药研究与实践, 2013, 27(1):85-88.
[5]陈莉敏,刘洋,李光文,等.姜黄素金属配合物的合成、表征和抗肿瘤活性研究[J].中国新药杂志, 2008(19):1676-1678.
[6]吴明亮.桑色素锌(Ⅱ)、镉(Ⅱ)、钴(Ⅱ)配合物与DNA的作用机理及DNA电化学传感器的研究[D].青岛:青岛科技大学, 2009.
[7]钱俊臻,王伯初,谭君,等.黄酮类化合物的金属配合物及其药理作用[J].中国药理学通报, 2012, 28(8):1058-1062.
[8]周晶,王进义,龚国权,等.槲皮素合铜(Ⅱ)配合物与DNA相互作用的研究[J].兰州大学学报, 2000(6):131-133.