摘要
目的研究染料木素-锌配合物的合成,并比较染料木素与锌金属离子配位前后的抗癌活性变化。方法以天然活性成分染料木素为配体与锌金属离子配位,合成染料木素-锌配合物。利用红外光谱、紫外光谱、核磁共振等方法对配合物进行表征。利用MTT法比较染料木素与锌金属离子配位前后的抗癌活性变化。结果合成得到的染料木素-锌配合物为淡黄色粉末,分子式为Zn(C15H9O5)2·2H2O,分子量为651。MTT试验结果表明,染料木素-锌配合物对MCF-7和H460细胞增殖抑制的效果明显高于其配体染料木素。结论染料木素-锌配合物的合成方法简便,其抗癌活性高于其配体染料木素。
Objective To study the synthesis of the genistein-zinc complexes and compare the change of anticancer activity before and after coordination between genistein and zinc metal ions. Methods Genistein-zinc complexes is prepared by the combination of nature compound genistein and zinc metal ions. The IR spectra, UV spectra, 1 HNMR are used to characterized the structure of genistein-zinc complexes. The MTT assay is used to compared the anticancer activities before and after coordination between genistein and zinc metal ions. Results The results show that the genistein-zinc complexes have been gotten, light yellow solid, whose molecular formula is Zn(C15 H9 O5)2·2 H2 O and molecular weidght(m/z) is 651. The MTT results are showed that the anticancer activity of genistein-zinc complexes on MCF-7 cells and H460 cells are obviously higher than its ligand genistein. Conclusion The synthesis method of genistein-zinc complexes is simple and its anticancer activity is higher than its ligand genistein.
引文
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