大鼠心肌细胞的分离培养
摘要
<正>在利用分子生物学技术来研究心脏生理学方面,培养心肌细胞正在成为一个日益重要的技术[1-5]。急性分离的心肌细胞无法显示基因表达的变化,因此,培养心肌细胞是必要的。成熟心肌细胞通过闰盘和细胞外基质紧密连接,因此很难分离。直到1976年,分离心室心肌细胞的技术才被报道[6]。随着分子生物学和细胞生物学的发展,细胞水平的研究口益受到重视,对心肌细胞分离培养要求也越来越严格,本文对近年来心肌细胞分离培养作一综述,现报告如下。
引文
[1]Elena N.Dedkova,Lothar A.Blatter.Measuring mitochondrial function in intact cardiac myocytes[J].Journal of Molecular and Cellular Cardiology,2012,52(1):48-61.
[2]Ching-Chieh Su,Jia-Ying Yang,Hsin-Ban Leu,et al.Mitochondrial Akt-regulated mitochondrial apoptosis signaling in cardiac muscle cells[J].Physiol Heart Circ Physiol,2012,302(3):716-723.
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[4]樊理华,韩新,卢向红,等.阿霉素对大鼠原代心肌细胞凋亡的影响[J].中华全科医学,2013,11(8):1167-1168.
[5]李小娟,王海英,喻田.乳化异氟醚后处理对成年大鼠缺氧/复氧心肌细胞超微结构的影响[J].贵州医药,2012,36(9):771-775.
[6]Powell T,TWIST VW.A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and atolerance to calcium[J].Biochem Biophys Res Commum,1976,72(1):327-333.
[7]Miteheson J S,Hancox J C,Levi A J.Cultured adult cardiac myocytes:Future application,culture methods,morphological and electrophysiological properties[J].Cardiovasc Res,1998,39(2):280-300.
[8]Berger HJ,Prasad SK,Davidoff AJ,et al.Continual electric field stimulation preserves contractile function of adult ventricular myocytes in primary culture[J].Am J Physio1Heart Circ Physiol,1994,266(1Pt2):H341-H349.
[9]lika Pinz,Ming Zhu,Ulrike Mende,et al.An Improved Isolation Procedure for Adult Mouse Cardiomyocytes[J].Cell Biochem Biophys,2011,61(1):93-101.
[10]William E.Louch,Katherine A.Sheehan C,Beata M.Wolska.Methods in cardiomyocyte isolation,culture,and gene transfer[J].Journal of Molecular and Cellular Cardiology,2011,51(3):288-298.
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[12]宫海滨,王洁,刘颖,等.β2-AR过表达对大鼠心肌细胞存活率的影响及机制[J].中华全科医学,2009,7(4):333-334.
[13]张紫冠,李卫华,黄峥嵘,等.SD大鼠心肌细胞分离方法探索[J].心血管康复医学杂志,2010,19(3):274-278.
[14]陈吉球.心肌细胞分离术[J].中国病理生理学杂志,1999,15(5):475-477.
[15]石晓路,柳絮,郭会彩,等.大鼠心肌细胞分离方法的改进[J].中国药理学通报,2010,26(5):687-690.
[16]李德,唐兵,杨大春,等.Ⅱ型胶原酶加压灌流提高成年大鼠心肌细胞分离效率[J].中国实验动物学报,2011,19(4):150-152.
[17]Egorova MV,Afanaspev SA,Popov SV.A simple method for isolation of cardiomyocytes from adult rat heart[J].Bull Exp Biol Med,2005,140(3):370-373.
[18]Burrows MT.Rhythmical activity of isolated heart muscle cells in vitro[J].Science,1912,36(916):90-92.
[19]宋智钢,刘维永.成熟心肌细胞培养技术及其应用进展[J].临床心血管病杂志,2001,17(8):383-384.
[20]Koch-Schneidenann S,Gehr P,Rutishauser B,et al.Attachment of adult rat cardiomyocytes on laminin and two laminin fragments[J].Struct Bio,1994,113(2):107-116.
[21]Cerbai E,Sartiani L,De Paoli P,et al.Isolated cardiac cells for electropharmacological studies[J].Pharmacol Res,2000,42(1):1-8.
[22]RenJ,Wold LE.Measurement of Cardiac Mechanical Function in Isolated Ventricular Myoeytes from Rats and Mice by Computerized Video-Based Imaging[J].Biol Proced Online,2001,3:43-53.
[23]Louch WE,Hake J,Jφlle OF,et al.Control of Ca2+release by action potential configuration in normal and failing murine cardiomyocytes[J].Biophys,2010,99(5):1377-1386.
[24]王长华.心肌细胞的培养及注意事项[J].中国心脏起搏与心电生理杂志,2003,17(3):203-231.
[25]Yanmauchi Y,Harada A,Kawahara K.Changes in the fluctuation of interbeat intervals in spontaneously beating cultured cardiac myoeytes:experimental and modeling studies[J].Biol Cybern,2002,86(2):147-154.
[26]施雨露,李晓辕,曹美娜,等.新生大鼠心肌细胞和心肌成纤维细胞的同时分离[J].中国组织工程研究,2013,17(24):4414-4420.
[2]Ching-Chieh Su,Jia-Ying Yang,Hsin-Ban Leu,et al.Mitochondrial Akt-regulated mitochondrial apoptosis signaling in cardiac muscle cells[J].Physiol Heart Circ Physiol,2012,302(3):716-723.
[3]Feng Wei,Ting-Zhong,Wang Jing Zhang,et al.Mamesenchymal stem cells neither fully acquire the electrophysiological properties of mature cardiomyocytes nor promote ventricular arrhythmias in infarcted rats[J].Basic Res Cardiol,2012,107(4):274.
[4]樊理华,韩新,卢向红,等.阿霉素对大鼠原代心肌细胞凋亡的影响[J].中华全科医学,2013,11(8):1167-1168.
[5]李小娟,王海英,喻田.乳化异氟醚后处理对成年大鼠缺氧/复氧心肌细胞超微结构的影响[J].贵州医药,2012,36(9):771-775.
[6]Powell T,TWIST VW.A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and atolerance to calcium[J].Biochem Biophys Res Commum,1976,72(1):327-333.
[7]Miteheson J S,Hancox J C,Levi A J.Cultured adult cardiac myocytes:Future application,culture methods,morphological and electrophysiological properties[J].Cardiovasc Res,1998,39(2):280-300.
[8]Berger HJ,Prasad SK,Davidoff AJ,et al.Continual electric field stimulation preserves contractile function of adult ventricular myocytes in primary culture[J].Am J Physio1Heart Circ Physiol,1994,266(1Pt2):H341-H349.
[9]lika Pinz,Ming Zhu,Ulrike Mende,et al.An Improved Isolation Procedure for Adult Mouse Cardiomyocytes[J].Cell Biochem Biophys,2011,61(1):93-101.
[10]William E.Louch,Katherine A.Sheehan C,Beata M.Wolska.Methods in cardiomyocyte isolation,culture,and gene transfer[J].Journal of Molecular and Cellular Cardiology,2011,51(3):288-298.
[11]王如兴,蒋文平.正常耐钙Spraque Dawley大鼠心室肌细胞分离方法及体会[J].中国心脏起搏与心电生理杂志,2007,21(1):59-62.
[12]宫海滨,王洁,刘颖,等.β2-AR过表达对大鼠心肌细胞存活率的影响及机制[J].中华全科医学,2009,7(4):333-334.
[13]张紫冠,李卫华,黄峥嵘,等.SD大鼠心肌细胞分离方法探索[J].心血管康复医学杂志,2010,19(3):274-278.
[14]陈吉球.心肌细胞分离术[J].中国病理生理学杂志,1999,15(5):475-477.
[15]石晓路,柳絮,郭会彩,等.大鼠心肌细胞分离方法的改进[J].中国药理学通报,2010,26(5):687-690.
[16]李德,唐兵,杨大春,等.Ⅱ型胶原酶加压灌流提高成年大鼠心肌细胞分离效率[J].中国实验动物学报,2011,19(4):150-152.
[17]Egorova MV,Afanaspev SA,Popov SV.A simple method for isolation of cardiomyocytes from adult rat heart[J].Bull Exp Biol Med,2005,140(3):370-373.
[18]Burrows MT.Rhythmical activity of isolated heart muscle cells in vitro[J].Science,1912,36(916):90-92.
[19]宋智钢,刘维永.成熟心肌细胞培养技术及其应用进展[J].临床心血管病杂志,2001,17(8):383-384.
[20]Koch-Schneidenann S,Gehr P,Rutishauser B,et al.Attachment of adult rat cardiomyocytes on laminin and two laminin fragments[J].Struct Bio,1994,113(2):107-116.
[21]Cerbai E,Sartiani L,De Paoli P,et al.Isolated cardiac cells for electropharmacological studies[J].Pharmacol Res,2000,42(1):1-8.
[22]RenJ,Wold LE.Measurement of Cardiac Mechanical Function in Isolated Ventricular Myoeytes from Rats and Mice by Computerized Video-Based Imaging[J].Biol Proced Online,2001,3:43-53.
[23]Louch WE,Hake J,Jφlle OF,et al.Control of Ca2+release by action potential configuration in normal and failing murine cardiomyocytes[J].Biophys,2010,99(5):1377-1386.
[24]王长华.心肌细胞的培养及注意事项[J].中国心脏起搏与心电生理杂志,2003,17(3):203-231.
[25]Yanmauchi Y,Harada A,Kawahara K.Changes in the fluctuation of interbeat intervals in spontaneously beating cultured cardiac myoeytes:experimental and modeling studies[J].Biol Cybern,2002,86(2):147-154.
[26]施雨露,李晓辕,曹美娜,等.新生大鼠心肌细胞和心肌成纤维细胞的同时分离[J].中国组织工程研究,2013,17(24):4414-4420.