miRNA-1271对人乳腺癌细胞增殖的影响
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摘要
目的探讨miRNA-1271对人乳腺癌细胞增殖的影响。方法采用RT-PCR法检测80例乳腺癌组织及癌旁正常组织中miRNA-1271的表达。采用脂质体转染法将miRNA-1271模拟物转染至乳腺癌细胞株MCF-7和MDA-MB-231中,应用CCK-8实验、克隆形成实验、流式细胞术、动物实验等探究miRNA-1271对乳腺癌细胞增殖、凋亡的影响。结果乳腺癌组织中miRNA-1271相对表达量较癌旁正常组织明显降低(P<0.05)。miRNA-1271表达上调后,MCF-7和MDA-MB-231乳腺癌细胞的吸光度值明显降低(均P<0.05),克隆形成数明显减少(均P<0.05),细胞凋亡率明显增高(均P<0.05)。动物实验结果显示,miRNA-1271在体内亦能抑制MCF-7乳腺癌细胞的增殖。结论 miRNA-1271在乳腺癌组织中低表达;上调miRNA-1271表达,能抑制乳腺癌细胞的增殖,促进凋亡的发生,具有抑癌作用。
Objective To investigate the effect of mi RNA-1271 on proliferation of human breast cancer cells. Methods The expression levels of mi RNA-1271 were detected with RT-PCR in 80 samples of breast cancer and pericancerous breast tissues. Human breast cancer MCF-7 and MDA-MB-231 cells were transfected with mi RNA-1271 mimics, the proliferation of breast cancer cells was determined by CCK-8 and colony formation assays, cell apoptosis was examined by flow cytometry. The mi RNA-1271-transfected breast cancer MCF-7 cells were inoculated in nude mice and the growth of transplanted tumors was measured. Results The expression level of mi RNA-1271 in breast cancer tissues were significantly lower than that in pericancerous breast tissues(P <0.05). CCK-8 assay showed that the OD490 value was significantly lower in mi RNA-1271-expressing breast cancer cells(P<0.05). The number of clone formation cells was lower and the apoptosis rate was significantly higher than those in control group(P<0.05). Animal experiments showed that mi RNA-1271 also inhibited the growth of MCF-7 breast cancer in vivo.Conclusion mi RNA-1271 is down-regulated in breast cancer tissues. Up-regulated mi RNA-1271 significantly inhibits the proliferation of breast cancer cells and increases cell apoptosis.
Objective To investigate the effect of mi RNA-1271 on proliferation of human breast cancer cells. Methods The expression levels of mi RNA-1271 were detected with RT-PCR in 80 samples of breast cancer and pericancerous breast tissues. Human breast cancer MCF-7 and MDA-MB-231 cells were transfected with mi RNA-1271 mimics, the proliferation of breast cancer cells was determined by CCK-8 and colony formation assays, cell apoptosis was examined by flow cytometry. The mi RNA-1271-transfected breast cancer MCF-7 cells were inoculated in nude mice and the growth of transplanted tumors was measured. Results The expression level of mi RNA-1271 in breast cancer tissues were significantly lower than that in pericancerous breast tissues(P <0.05). CCK-8 assay showed that the OD490 value was significantly lower in mi RNA-1271-expressing breast cancer cells(P<0.05). The number of clone formation cells was lower and the apoptosis rate was significantly higher than those in control group(P<0.05). Animal experiments showed that mi RNA-1271 also inhibited the growth of MCF-7 breast cancer in vivo.Conclusion mi RNA-1271 is down-regulated in breast cancer tissues. Up-regulated mi RNA-1271 significantly inhibits the proliferation of breast cancer cells and increases cell apoptosis.
引文
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[2]Chan CWH,Law BMH,So WKW,et al.Novel Strategies on Personalized Medicine for Breast.Cancer Treatment:An Update[J].Int J Mol Sci,2017,18(11):E2423.DOI:10.3390/ijms18112423.
[3]Zaleska K.miRNA-Therapeutic tool in breast cancer?Where are we now?[J].Rep Pract Oncol Radiother,2015,20(2):79-86.DOI:10.1016/j.rpor.2014.10.009.
[4]Rupaimoole R,Calin GA,Lopez-Berestein G,et al.miRNA Deregulation in Cancer Cells and the Tumor Microenvironment[J].Cancer Discov,2016,6(3):235-246.DOI:10.1158/2159-8290.
[5]Ades F,Tryfonidis K,Zardavas D.The past and future of breast cancer treatment-from the papyrus to individualised treatment approaches[J].Ecancermedicalscience,2017,11:746.DOI:10.3332/ecancer.2017.746.
[6]Mulrane L,Mc Gee SF,Gallagher WM,et al.miRNA dysregulation in breast cancer[J].Cancer Res,2013,73(22):6554-6562.DOI:10.1158/0008-5472.
[7]Schoepp M,Strose AJ,Haier J.Dysregulation of miRNA Expression in Cancer Associated Fibroblasts(CAFs)and Its Consequences on the Tumor Microenvironment[J].Cancers(Basel),2017,9(6):e54.DOI:10.3390/cancers9060054.
[8]Xu Z,Huang C,Hao D.MicroRNA-1271 inhibits proliferation and promotes apoptosis of multiple myeloma cells through inhibiting smoothened-mediated Hedgehog signaling pathway[J].Oncol Rep,2017,37(2):1261-1269.DOI:10.3892/or.2016.5304.
[9]Li C,Jiang Y,Miao R,et al.MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP4A1/c-Src axis[J].Int J Oncol,2018,52(2):536-546.DOI:10.3892/ijo.2017.4224.
[10]Zhen Z,Niu XM,Li C,et al.Inhibition of the growth of non-small cell lung cancer by miRNA-1271[J].Am J Transl Res,2015,7(10):1917-1924.DOI:10.26355/eurrev_201802_14293.
[11]Li J,Xu J,Yan X,et al.Suppression of Capn4 by microRNA-1271 impedes the proliferation and invasion of colorectal cancer cells[J].Biomed Pharmacother,2018,99:162-168.DOI:10.1016/j.biopha.2017.12.107.
[12]Zhong J,Liu Y,Xu Q,et al.Inhibition of DIXDC1 by microRNA-1271suppresses the proliferation and invasion of prostate cancer cells[J].Biochem Biophys Res Commun,2017,484(4):794-800.DOI:10.1016/j.bbrc.2017.01.169.
[13]Lim B,Kim HJ,Heo H,et al.Epigenetic silencing of miR-1271 enhances MEK1 and TEAD4 expression in gastric cancer[J].Cancer Med,2018.DOI:10.1002/cam4.1605.[Epub ahead of print]
[14]Finlay D,Teriete P,Vamos M,et al.Inducing death in tumor cells:roles of the inhibitor of apoptosis proteins[J].F1000Res,2017,6:587.DOI:10.12688/f1000research.10625.1.
[15]Qin A,Zhu J,Liu X,et al.miRNA-1271 inhibits cellular proliferation of hepatocellular carcinoma[J].Oncology Letters,2017,14:6783-6788.DOI:10.26355/eurrev_201805_14966.
[16]Fan DM,Feng XS,Qi PW,et al.Forkhead factor FOXQ1 promotes TGF-βexpression and induces epithelial-mesenchymal transition[J].Mol Cell Biochem,2014,397:179-186.DOI:10.1007/s11010-014-2185-1.
[17]Du HY,Liu B.MiR-1271 as a tumor suppressor in breast cancer proliferation and progression via targeting SPIN1[J].Eur Rev Med Pharmacol Sci,2018,22(9):2697-2706.DOI:10.26355/eurrew_201805_14966.