补肾益元方对运动性低血睾酮大鼠HPG轴的影响及有效成分测定
摘要
目的探讨补肾益元方对运动性低血睾酮大鼠HPG轴的影响,并测定方中有效成分。方法 40只大鼠随机分为空白组、模型组、淫羊藿提取物组(阳性对照,0.70 g/kg)及补肾益元方低、高剂量组(0.35、0.70 g/kg),每组8只。荧光PCR、Western blot法检测脑组织卵泡刺激素(FSH)、黄体生成素(LH)、促性腺激素释放激素(GnRH)mRNA、蛋白表达,HPLC法测定人参皂苷、淫羊藿苷含有量。结果大强度运动后,给药组大鼠HPG轴中FSH、LH、GnRH mRNA、蛋白表达均有不同程度上升。同时,每粒中药复方胶囊中分别含人参总皂苷、淫羊藿苷43.57、5.03 mg。结论补肾益元方能调控大鼠HPG轴,可为中药抗运动性低血睾酮提供理论依据,建议运动员服用剂量为1次4粒,每天3次。
引文
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[2] Crewther B T, Cook C, Cardinale M, et al. Two emerging concepts for elite athletes: the short-term effects of testosterone and cortisol on the neuromuscular system and the dose-response training role of these endogenous hormones[J]. Sports Med, 2011, 41(2): 103-123.
[3] 杨丽娟,周惠芳.补肾中药对H-P-O轴干预的实验研究进展[J].中国中医基础医学杂志,2015,21(3):370-371,374.
[4] 周志宏,刘建红,王奎,等.补肾益元中药对运动性疲劳肾气不足证及运动性低血睾酮的影响[J].中国运动医学杂志,2004,23(1):96-98.
[5] 周志宏,石幼琪,刘建红,等.补肾益元中药对运动员的抗疲劳作用[J].中国临床康复,2003,7(18):2628-2629.
[6] 徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,2002.
[7] 叶剑飞,余闽,岑浩望.过度训练的病理生理及康复Ⅰ.大鼠过度训练模型的建立[J].中国运动医学杂志,1992,11(1):15-21,64.
[8] Alvarado L C,Muller M N, Thompson M E, et al. The paternal provisioning hypothesis: effects of workload and testosterone production on men’s musculature[J]. Am J Phys Anthropol,2015,158(1): 19-35.
[9] Engi S A, Planeta C S, Crestani C C. Correction: effect of voluntary ethanol consumption combined with testosterone treatment on cardiovascular function in rats: influence of exercise training[J]. PLoS One, 2016, 11(3): 0151749.
[10] Dismukes A R,Johnson M M,Vitacco M J,et al. Coupling of the HPA and HPG axes in the context of early life adversity in incarcerated male adolescents[J]. Dev Psychobiol, 2015, 57(6): 705-718.
[11] Camille Melón L,Maguire J. GABAergic regulation of the HPA and HPG axes and the impact of stress on reproductive function[J]. J Steroid Biochem Mol Biol, 2016, 160: 196-203.
[12] 徐晓阳,冯炜权,曹建民,等.运动性低血睾酮及补肾中药对大鼠能量代谢某些指标的影响[J].中国运动医学杂志,2000,19(3):282-284,290.
[13] 李艳,于涛,苗明三.淫羊藿的化学、药理与临床应用分析[J].中医学报,2017,32(4):619-622.
[14] 黄文聪.运动应激对HPG轴下丘脑-垂体系统的影响和适应[J].体育科技,2006,27(4):53-55.
[15] 张福生,高兴国,周宗惠.中药抗运动性疲劳机制的研究综述[J].体育世界(学术版), 2012(10):22-23.
[16] 蔡德培,张炜.补肾中药对下丘脑GnRH、垂体FSH、LH及成骨细胞BGP基因表达的调节作用[J].中医杂志,2002,43(3):221-223.