基于单味药-配伍的合欢花与酸枣仁水提取物对抑郁性失眠动物模型的影响
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摘要
目的对酸枣仁、合欢花水提取物单味药和配伍应用进行抗抑郁性失眠研究,探讨其配伍应用及作用机制。方法采用抑郁性失眠动物模型,观察酸枣仁、合欢花水提取物单味药和配伍应用对行为学、海马组织内单胺神经递质和氨基酸神经递质含量及比值的影响。结果酸枣仁-合欢花提取物高、低剂量组及其单味药提取物高、低剂量组与模型组比较,旷场实验水平得分与垂直得分都明显提高(P <0.01,P <0.05)或有升高趋势,海马组织中单胺神经递质含量也明显增高(P <0.001,P <0.01)或有增高趋势,氨基酸神经递质Glu含量及Glu/GABA明显降低(P <0.001,P <0.01,P <0.05)或有降低趋势,GABA含量明显增高(P <0.01,P <0.05)或有增高趋势。结论酸枣仁、合欢花提取物单独或配伍应用,都能发挥改善抑郁性失眠的功效,并以配伍应用更佳。
Objective To study the use of water extract of Flos Albiziae and Semen Ziziphi Spinosae on anti-sleep deprivation depression based on single herb and compatibility, and to probe into the effect and mechanism of action of compatibility. Methods We adopted sleep deprivation depressionanimal model, to observe the effecton behavio ristics,monamineneurotransmitter content and amino acid neurotransmitters content and their ratio in hippocampus of the animals with the use of water extract of Flos Albiziae and Semen Ziziphi Spinosae based on single herb and compatibility. Results Compared with the model group, horizontalscore and verticalscore in open field test were obviously enhanced of the rats in Flos Albiziae and Semen Ziziphi Spinosae compatibility high-dose and low-dose groups and Flos Albiziae and Semen Ziziphi Spinosae single herb high-dose and low-dose groups(P < 0.01, P < 0.05)or had rising trend, and their monamine neurotransmitter content in hippocampus were obviously rised(P < 0.001,P < 0.01) or had rising trend, and their amino acid neurotransmitters content and their ratio in hippocampus were obviously reduced(P < 0.001, P < 0.01, P < 0.05) or had lower trend. The content of GABA increased significantly(P < 0.01, P < 0.05). Conclusion Whether compatibility use or single herb use of water extract of Flos Albiziae and Semen Ziziphi Spinosae, they all could develope anti-sleep deprivation depression effect. Compatibility use was better than single herb use.
Objective To study the use of water extract of Flos Albiziae and Semen Ziziphi Spinosae on anti-sleep deprivation depression based on single herb and compatibility, and to probe into the effect and mechanism of action of compatibility. Methods We adopted sleep deprivation depressionanimal model, to observe the effecton behavio ristics,monamineneurotransmitter content and amino acid neurotransmitters content and their ratio in hippocampus of the animals with the use of water extract of Flos Albiziae and Semen Ziziphi Spinosae based on single herb and compatibility. Results Compared with the model group, horizontalscore and verticalscore in open field test were obviously enhanced of the rats in Flos Albiziae and Semen Ziziphi Spinosae compatibility high-dose and low-dose groups and Flos Albiziae and Semen Ziziphi Spinosae single herb high-dose and low-dose groups(P < 0.01, P < 0.05)or had rising trend, and their monamine neurotransmitter content in hippocampus were obviously rised(P < 0.001,P < 0.01) or had rising trend, and their amino acid neurotransmitters content and their ratio in hippocampus were obviously reduced(P < 0.001, P < 0.01, P < 0.05) or had lower trend. The content of GABA increased significantly(P < 0.01, P < 0.05). Conclusion Whether compatibility use or single herb use of water extract of Flos Albiziae and Semen Ziziphi Spinosae, they all could develope anti-sleep deprivation depression effect. Compatibility use was better than single herb use.
引文
[1]DEMYTTENAERE K,BRUFFAERTS R,POSADA-VILLAJ,et al.Prevalenceseverityand unmet need for treatment of mental disorders in the World Health Organization World Mental Health Surveys[J].The Journal of theAmerican Medical Association,2004,291(21):2581-2590.
[2]应达时,王中男,王若男,等.舒郁颗粒对抑郁模型大鼠行为学及海马脑源性神经营养因子表达的影响[J].吉林中医药,2016,36(10):1033-1035.
[3]张继辉,刘亚平,潘集阳.失眠与抑郁关系2008-2013年研究进展及存在问题[J].中国心理卫生杂志,2015,29(2):81-86.
[4]XIANG Y T,MA X,CAI Z J,et al.The prevalence of insomnia,itssociademograghic and clinical correlates andtreatmentin rural and urban regions of Beijing,China:ageneral population based survey[J].Sleep,2008,31(12):1655-1662.
[5]LOPEZ-RODRIGUEZ F,KIM J,POLAND R E.Total sleep deprivation decreases immobility in the forced-swim test[J].Neuropsychophannacology:official publication of the American College of Neuropsychophannacology,2004,29(6):1105-1111.
[6]杨奕,乔卫,刘婧姝,等.酸枣仁抗抑郁活性组分配伍的研究[J].时珍国医国药,2012,23(1):7-8.
[7]赵启铎,舒乐新,王颖,等.酸枣仁油对行为绝望小鼠模型的影响[J].中国实验方剂学杂志,2011,17(18):190-192.
[8]李作平,赵丁,任雷鸣,等.合欢花抗抑郁作用的药理实验研究初探[J].河北医科大学学报,2003,24(4):214-216.
[9]向春,严铸云,李晓华,等.5种川产合欢花抗抑郁作用比较研究[J].时珍国医国药,2006,17(7):1168-1169.
[10]施学丽,夏猛,冯秋瑜,等.对药酸枣仁-合欢花对抑郁患者生活质量及神经-内分泌-免疫网络的影响[J].时珍国医国药,2016,27(10):2348-2350.
[11]施学丽,郭超峰,范丽丽,等.对药酸枣仁-合欢花对老年抑郁症患者生活质量的影响[J].世界科学技术-中医药现代化,2013,15(9):1933-1936.
[12]施学丽,张永全,蒋春雷.对药酸枣仁-合欢花治疗抑郁症的临床研究[J].广西中医药,2016,39(3):21-23.
[13]孙秀萍,王琼,石哲,等.动物行为实验方法学研究的回顾与展望[J].中国比较医学杂志,2018,28(3):1-7.
[14]林晓春,李云鹏,卞艳芳,等.大鼠旷场实验指标检测及参考值的探讨[J].毒理学杂志,2010,24(3):224-225.
[15]赵洪庆,韩远山,柳卓,等.焦虑性抑郁模型大鼠脑区单胺递质含量与神经因子表达的变化[J].中国实验动物学报,2017,25(4):373-379.
[16]王冰梅,许峰,王中男.熟眠胶囊对失眠模型大鼠脑内不同组织5-HT1α、5-HT2α受体表达的影响[J].吉林中医药,2015,35(3):286-288.
[17]乎宝力格,苏日古嘎,高玉峰.蒙医灸疗对失眠模型大鼠行为学及脑组织5-HT的影响[J].吉林中医药,2018,38(12):1426-1428.
[18]周庆莹,刘宏岩.解郁开心丹对抑郁模型大鼠脑内单胺类神经递质的影响[J].吉林中医药,2015,35(3):283-285.
[19]NUTTD D J,STAHL S M.Searching for perfect sleep:the continuing evolution of GABAA receptor modulators as Hypnotics[J].Psychopharm acol,2010,24(11):1601-1612.
[20]刘娟娟.失眠合并脑梗死患者血浆GABA、Glu、Ach水平的临床研究[J].中国医学创新,2019,16(3):37-40.
[21]甘雨,马进,乔敏,等.安神脐贴对实验性失眠模型大鼠自主活动及血清GABA、NE含量的影响[J].实验动物科学,2018,35(4):45-48.
[2]应达时,王中男,王若男,等.舒郁颗粒对抑郁模型大鼠行为学及海马脑源性神经营养因子表达的影响[J].吉林中医药,2016,36(10):1033-1035.
[3]张继辉,刘亚平,潘集阳.失眠与抑郁关系2008-2013年研究进展及存在问题[J].中国心理卫生杂志,2015,29(2):81-86.
[4]XIANG Y T,MA X,CAI Z J,et al.The prevalence of insomnia,itssociademograghic and clinical correlates andtreatmentin rural and urban regions of Beijing,China:ageneral population based survey[J].Sleep,2008,31(12):1655-1662.
[5]LOPEZ-RODRIGUEZ F,KIM J,POLAND R E.Total sleep deprivation decreases immobility in the forced-swim test[J].Neuropsychophannacology:official publication of the American College of Neuropsychophannacology,2004,29(6):1105-1111.
[6]杨奕,乔卫,刘婧姝,等.酸枣仁抗抑郁活性组分配伍的研究[J].时珍国医国药,2012,23(1):7-8.
[7]赵启铎,舒乐新,王颖,等.酸枣仁油对行为绝望小鼠模型的影响[J].中国实验方剂学杂志,2011,17(18):190-192.
[8]李作平,赵丁,任雷鸣,等.合欢花抗抑郁作用的药理实验研究初探[J].河北医科大学学报,2003,24(4):214-216.
[9]向春,严铸云,李晓华,等.5种川产合欢花抗抑郁作用比较研究[J].时珍国医国药,2006,17(7):1168-1169.
[10]施学丽,夏猛,冯秋瑜,等.对药酸枣仁-合欢花对抑郁患者生活质量及神经-内分泌-免疫网络的影响[J].时珍国医国药,2016,27(10):2348-2350.
[11]施学丽,郭超峰,范丽丽,等.对药酸枣仁-合欢花对老年抑郁症患者生活质量的影响[J].世界科学技术-中医药现代化,2013,15(9):1933-1936.
[12]施学丽,张永全,蒋春雷.对药酸枣仁-合欢花治疗抑郁症的临床研究[J].广西中医药,2016,39(3):21-23.
[13]孙秀萍,王琼,石哲,等.动物行为实验方法学研究的回顾与展望[J].中国比较医学杂志,2018,28(3):1-7.
[14]林晓春,李云鹏,卞艳芳,等.大鼠旷场实验指标检测及参考值的探讨[J].毒理学杂志,2010,24(3):224-225.
[15]赵洪庆,韩远山,柳卓,等.焦虑性抑郁模型大鼠脑区单胺递质含量与神经因子表达的变化[J].中国实验动物学报,2017,25(4):373-379.
[16]王冰梅,许峰,王中男.熟眠胶囊对失眠模型大鼠脑内不同组织5-HT1α、5-HT2α受体表达的影响[J].吉林中医药,2015,35(3):286-288.
[17]乎宝力格,苏日古嘎,高玉峰.蒙医灸疗对失眠模型大鼠行为学及脑组织5-HT的影响[J].吉林中医药,2018,38(12):1426-1428.
[18]周庆莹,刘宏岩.解郁开心丹对抑郁模型大鼠脑内单胺类神经递质的影响[J].吉林中医药,2015,35(3):283-285.
[19]NUTTD D J,STAHL S M.Searching for perfect sleep:the continuing evolution of GABAA receptor modulators as Hypnotics[J].Psychopharm acol,2010,24(11):1601-1612.
[20]刘娟娟.失眠合并脑梗死患者血浆GABA、Glu、Ach水平的临床研究[J].中国医学创新,2019,16(3):37-40.
[21]甘雨,马进,乔敏,等.安神脐贴对实验性失眠模型大鼠自主活动及血清GABA、NE含量的影响[J].实验动物科学,2018,35(4):45-48.