高效DMF降解菌群的降解特性及影响因素
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摘要
从浙江省某皮革厂生化池内的活性污泥中培养得到一组高效利用二甲基甲酰胺(DMF)作为碳源和氮源生长的混合菌群。经群落结构鉴定,菌群主要包含Brevundimonas sp.、Flavobacterium sp.、Nocardioidaceae sp.、Brevibacillus parabrevis和Stenotrophomonas sp.等。批式试验表明,DMF降解菌群的最佳接种率为30%,在pH为5.0～9.0的范围内均有氧化效果,最适pH为6.0,在48h内可将培养基中0.2%的DMF完全分解。菌群对DMF的最大耐受体积分数为6%,体系中氨氮浓度超过2000mg/L时,菌群仍具备DMF分解能力,硝酸盐浓度低于450mg/L时对降解效果影响较小。经7天驯化,菌群还可具备对同系物二甲基乙酰胺(DMAC)的分解能力。
Bacterial communities were obtained from activated sludge of a tannery in Zhejiang province,which grew by utilizing DMF(N,N-dimethylformamide) as carbon and nitrogen source. The community structure indicated that the microflora mainly consisted of: Brevundimonas sp., Flavobacterium sp.,Nocardioidaceae sp., Brevibacillus parabrevis and Stenotrophomonas sp..The optimal inoculation rate was30% and in the range of pH was 5.0—9.0, the microflora showed the oxidation effect and 0.2% of DMF in the medium could be completely decomposed within 48 hours when the optimal pH was 6.0. The maximal tolerable concentration of DMF was 6%. The concentration of ammonia nitrogen in the system exceeded2000 mg/L, the strains still had the ability to decompose DMF, and the nitrate concentration lower than450 mg/L had little effect on the degradation effect. After 7 days of acclimation, the bacteria could decompose DMAC(dimethylacetamide).
Bacterial communities were obtained from activated sludge of a tannery in Zhejiang province,which grew by utilizing DMF(N,N-dimethylformamide) as carbon and nitrogen source. The community structure indicated that the microflora mainly consisted of: Brevundimonas sp., Flavobacterium sp.,Nocardioidaceae sp., Brevibacillus parabrevis and Stenotrophomonas sp..The optimal inoculation rate was30% and in the range of pH was 5.0—9.0, the microflora showed the oxidation effect and 0.2% of DMF in the medium could be completely decomposed within 48 hours when the optimal pH was 6.0. The maximal tolerable concentration of DMF was 6%. The concentration of ammonia nitrogen in the system exceeded2000 mg/L, the strains still had the ability to decompose DMF, and the nitrate concentration lower than450 mg/L had little effect on the degradation effect. After 7 days of acclimation, the bacteria could decompose DMAC(dimethylacetamide).
引文
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[3]胡湖生,杨明德,叶血清,等.萃取-吸附法处理二甲基甲酰胺(DMF)废水的实验研究[J].环境科学研究,2004,17(4):40-43.HU H S,YANG M D,YE X Q,et al.Experimental study on treatment of dimethylformamide wastewater by extraction-adsorption method[J].Environmental Science Research,2004,17(4):40-43.
[4]张慧,叶长燊,林诚.机械搅拌萃取塔中氯仿萃取DMF的传质系数[J].环境工程学报,2011,5(1):103-107.ZHANG H,YE C Q,LIN C.The mass transfer coefficient of chloroform extraction DMF in mechanical stirring extraction tower[J].Journal of Environmental Engineering,2011,5(1):103-107.
[5]赵舜华,宋锡瑾,张景铸,等.合成革生产废水中DMF的节能回收新工艺[J].化工进展,2007,26(9):1347-1350.ZHAO S H,SONG X J,ZHANG J Z,et al.A new energy-saving recycling process for DMF in synthetic leather production wastewater[J].Chemical Industry and Engineering Progress,2007,26(9):1347-1350.
[6]LI S F,CHEN Q.Adsorption of N,N-dimethylformamide by macroporous chitosan membranes[C]//12th Asian Pacific Confederation of Chemical Engineering Congress(Vol.2,Environment and Green Processing).Xiamen,2008.
[7]付杰,李燕虎,叶长燊,等.DMF在大孔吸附树脂上的吸附热力学及动力学研究[J].环境科学学报,2012,32(3):639-644.FU J,LI Y H,YE C Q,et al.Adsorption thermodynamics and kinetics of DMF on macroporous adsorption resin[J].Chinese Journal of Environmental Science,2012,32(3):639-644.
[8]刘志国.DMF废水资源化无害化处理研究[D].南京:南京工业大学,2005.LIU Z G.Study on DMF wastewater resource utilization and harmless treatment[D].Nanjing:Nanjing University of Technology,2005.
[9]刘志国,杨祝红,郑仲,等.氧化剂增强TiO2纤维光催化降解DMF研究[J].环境科学,2006,27(1):47-50.LIU Z G,YANG Z H,ZHENG Z,et al.Study on photocatalytic degradation of DMF by oxidant-enhanced TiO2fiber[J].Environmental Science,2006,27(1):47-50.
[10]胡晓莲,王西峰.光/电/化学催化降解水中二甲基甲酰胺的反应特性[J].环境科学研究,2009,22(5):549-552.HU X L,WANG X F.Reaction characteristics of light/electric/chemical catalytic degradation of dimethylformamide in water[J].Research of Environmental Sciences,2009,22(5):549-552.
[11]GAVRILYUK A I.Degradation of dimethylformamide on the surface of the nanosized WO3films studied by infrared spectroscopy[J].Applied Surface Science,2016,377:56-65.
[12]GROSJEAN N,DESCOME C,BESSON M.Catalytic wet air oxidation of N,N-dimethylformamide aqueous solutions:deactivation of TiO2and ZrO2-supported noble metal catalysts[J].Applied Catalysis B:Environmental,2010,97(1/2):276-283.
[13]李微.非均相Fenton催化剂氧化降解废水中DMF技术研究[D].杭州:浙江大学,2014.LI W.Study on oxidative degradation of DMF in wastewater by heterogeneous fenton catalyst[D].Hangzhou:Zhejiang University,2014.
[14]CHROMEK J,KUPECE J,MAVAN P,et al.Moeglichkeiten der nachreinigung von abwaessern aus der kunstlederproduktion mit hilfe von Algen[J].Acta Hydrochimica et Hydrobiologica,1983,11(3):329-338.
[15]CARTER J,YOUNG D A.Biodegration of chmical plant wastewater containing dimethylformamide[J].Proc.Ind.Waste Conf.,1984,38(2):199-211.
[16]GHISALBA O,KUENZI M,SCHAR H.Biodegradation and utilization of N,N-dimethylformamide by specialized methylotrophs[J].Experientia,1986,42(1):108.
[17]SZTAJER H,LEJCZAK B,PAWLACZYKV M.Enzymatic activity of activated sludge in biological treatment of wastewater containing dimethylamine,dimethylformamide and methylethylketone[J].Acta Microbiologica Polonica,1984,33(2):171-178.
[18]赵骏,赵建国,陈秀荣,等.处理DMF废水连续流和SBR两系统污泥毒性对比研究[J].环境科学与技术,2015,38(5):125-129.ZHAO J,ZHAO J G,CHEN X R,et al.Comparative study on the toxicity of continuous DMF wastewater and SBR two-system sludge[J].Environmental Science and Technology,2015,38(5):125-129.
[19]徐明仙,林春绵,周红艺.超临界水氧化法改善有机污染物可生化性的研究[J].环境污染治理技术与设备,2002,3(3):24-26.XU M X,LIN C M,ZHOU H Y.Study on improving the biodegradability of organic pollutants by supercritical water oxidation[J].Environmental Pollution Control Technology and Equipment,2002,3(3):24-26.
[20]洪飞宇,李德生,韩丹,等.高级氧化/生物法去除制膜废水中的强有机溶剂[J].中国给水排水,2009,25(11):43-46.HONG F Y,LI D S,HAN D,et al.Removal of strong organic solvents in film wastewater by advanced oxidation/bio method[J].China Water&Wastewater,2009,25(11):43-46.
[21]杜海霞,王亚娥,李杰.Fenton氧化与铁炭微电解组合预处理DMF废水[J].环境工程学报,2014,8(9):3695-3703.DU H X,WANG Y E,LI J.Pretreatment of DMF wastewater by Fenton oxidation and iron-carbon microelectrolysis[J].Journal of Environmental Engineering,2014,8(9):3695-3703.
[22]邬莎娜,孙贤波,刘勇弟,等.Fenton法处理DMF废水及无机阴离子对反应的影响[J].华东理工大学学报(自然科学版),2017,43(1):70-75.WU S N,SUN X B,LIU Y D,et al.Fenton law affecting DMFwastewater and inorganic anions on the reaction[J].Process of East China University of Technology(Natural Science),2017,43(1):70-75.
[23]SCH?R H P,HOLZMANN W,RAMOS TOMBO G M,et al.Purification and characterization of N,N-dimethylformamidase from Pseudomonas sp.DMF 3/3[J].European Journal of Biochemistry,1986,158(3):469-475.
[24]OKAZAKI M,HAMADA T,FUJI H,et al.Development of poly(vinyl alcohol)hydrogel for waste water cleaning.Ⅱ.Treatment of N,N‐dimethylformamide in waste water with poly(vinyl alcohol)gel with immobilized microorganisms[J].Journal of Applied Polymer Science,1995,58(12):2243-2249.
[25]HASEGAWA Y,MATSUO M,SIGEMOTO Y,et al.Purification and characterization of N,N-dimethylformamidase from Alcaligenes sp.KUFA-1[J].Journal of Fermentation&Bioengineering,1997,84(6):543-547.
[26]VEERANAGOUDA Y,P V,EMMANUEL Paul,et al.Complete mineralisation of dimethylformamide by Ochrobactrum sp.DGVK1isolated from the soil samples collected from the coalmine leftovers[J].Applied Microbiology and Biotechnology,2006,71(3):369-475.
[27]URAKAMI T,KOBAYASHI H,ARAKI H.Isolation and identification of N,N-dimethylformamide-biodegrading bacteria[J].Journal of Fermentation and Bioengineering,1990,70(1):45-47.
[28]URAKAMI T,ARAKI H,OYANAGI H,et al.Paracoccus aminophilus sp.nov.and Paracoccus Aminovorans sp.nov.,which utilize N,N-dimethylformamide[J].Interntional Journal of Systematic Bacteriology,1990,40(3):287-291.
[29]SWAROOP S,SUGHOSH P,RAMANATHAN G.Biomineralization of N,N-dimethylformamide by Paracoccus sp.strain DMF[J].Journal of Hazardous Materials,2009,171(1/2/3):268-272.
[30]SIDDAVATTARN D,KAREGOUDAR T B,MUDDE S K,et al.Genome of a novel isolate of Paracoccus denitrificans capable of degrading N,N-dimethylformamide[J].Journal of Bacteriology,2011,193(19):5598-5599.
[31]SANJEEVKUMAR S,NAYAK A S,SANTOSHKUMAR M,et al.Paracoccus denitrificans SD1 mediated augmentation with indigenous mixed cultures for enhanced removal of N,N-dimethylformamide from industrial effluents[J].Biochemical Engineering Journal,2013,79(2):1-6.
[32]KIRANKUMAR B,KULKAMI G B,SANJEEVKUMAR S,et al.Influence of DMF-induced oxidative stress on membrane and periplasmic proteins in Paracoccus sp.SKG[J].Applied Biochemistry&Biotechnology,2014,173(5):1263-1273.
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