超高效液相色谱-质谱联用法测定不同粒径活血散透皮吸收液中4种成分含量
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摘要
目的比较粒径分别为0.150,0.075,0.048,0.038 mm的活血散透皮吸收前后的大黄素、黄柏碱、小檗碱、药根碱的含量,确定活血散的最佳粒径。方法采用超高效液相-质谱联用技术,对不同粒径活血散透皮吸收液24 h内大黄素、黄柏碱、小檗碱、药根碱的含量进行测定,比较各有效成分的含量,确定活血散的最佳粒径。结果粒径0.075 mm的活血散各有效成分含量较高,透皮吸收后各有效成分含量也较高。结论活血散以粒径0.075 mm的散剂最佳。
Objective To optimize the particle size of Huoxue powder by contents comparison of emodin,phellodendrine,berberine and jatrorrhizine before and after permeabilized skin absorption of Huoxue powder in different particle size of 0.150,0. 075,0. 048,0. 038 mm. Methods The contents of emodin,phellodendrine,berberine and jatrorrhizine in transparent absorbent fluid of Huoxue power in different particle size within 24 hours were measured by ultra performance liquid chromatography tandem mass spectrometry,and optimize its particle size by contents comparison of the effective components.Results The contents of the active components in Huoxue power with the particle size of 0.075 mm were high before and after percutaneous absorption. Conclusion Particle size of 0.075 mm is best for Huoxue powder.
Objective To optimize the particle size of Huoxue powder by contents comparison of emodin,phellodendrine,berberine and jatrorrhizine before and after permeabilized skin absorption of Huoxue powder in different particle size of 0.150,0. 075,0. 048,0. 038 mm. Methods The contents of emodin,phellodendrine,berberine and jatrorrhizine in transparent absorbent fluid of Huoxue power in different particle size within 24 hours were measured by ultra performance liquid chromatography tandem mass spectrometry,and optimize its particle size by contents comparison of the effective components.Results The contents of the active components in Huoxue power with the particle size of 0.075 mm were high before and after percutaneous absorption. Conclusion Particle size of 0.075 mm is best for Huoxue powder.
引文
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[14]胡梦迪,苏迪,范晓苏,等.月桂酰基苹果酸酯胶束电动色谱-质谱法同时测定牛黄上清片中5种生物碱[J].色谱,2012,30(12):1276-1281.
[15]邓雯.三七、西洋参两味中药材超微粉的制备工艺与质量评价研究[D].广州:广州中医药大学,2008:20-21.
[2]马微,王海波,陈冬东,等.高效液相色谱-串联质谱法同时测定减肥保健食品中酚酞和大黄素的方法研究[J].食品科技,2010,8(3):339-343.
[3]张艺竹,安叡,陈烨,等.LC-MS/MS同时测定一清胶囊9种成分的含量[J].药物分析杂志,2013,33(7):1197-1201,1209.
[4]刘俊红,王红,曲鹏飞,等.金黄膏中小檗碱体外透皮吸收研究[J].中成药,2014,36(1):65-69.
[5]李芸,闫治攀,魏舒畅,等.盐酸小檗碱、吴茱萸挥发油、大黄总蒽醌促进苦参碱透皮吸收的量效关系研究[J].中成药,2014,36(3):510-514.
[6]汪宏,王维,张建华,等.他克莫司软膏的体外透皮吸收研究[J].华西药学杂志,2014,29(2):119-121.
[7]肖学成,熊驰,彭秀菊,等.HPLC测定他克莫司软膏体外透皮吸收[J].中国现代应用药学,2014,31(7):839-843.
[8]梁学政,奉建芳,陈惠红,等.双柏凝胶剂中大黄素体外透皮吸收的实验研究[J].时珍国医国药,2010,21(1):160-161.
[9]李玲慧.双黄巴布剂的研制与体外透皮吸收研究[D].广州:广州中医药大学,2010:15.
[10]张倩,蔡丽芬,钟国跃,等.RP-HPLC法同时测定关黄柏中小檗碱、药根碱、巴马汀及黄柏酮含量的方法学研究[J].中国中药杂志,2010,35(16):2061-2064.
[11]匡艳辉,朱晶晶,王智民,等.一测多评法测定黄连中小檗碱、巴马汀、黄连碱、表小檗碱、药根碱含量[J].中国药学杂志,2009,44(5):390-394.
[12]董静,王弘,万乐人,等.高效液相色谱/电喷雾-离子阱-飞行时间质谱分析鉴定中药虎杖中的主要化学成分[J].色谱,2009,27(4):425-430.
[13]蒋海强,容蓉,吕青涛.大黄化学成分的液相色谱-质谱联用鉴别[J].时珍国医国药,2011,22(7):1705-1706.
[14]胡梦迪,苏迪,范晓苏,等.月桂酰基苹果酸酯胶束电动色谱-质谱法同时测定牛黄上清片中5种生物碱[J].色谱,2012,30(12):1276-1281.
[15]邓雯.三七、西洋参两味中药材超微粉的制备工艺与质量评价研究[D].广州:广州中医药大学,2008:20-21.