马铃薯StNF-Y核转录因子基因的克隆和生物信息学分析
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摘要
为了研究马铃薯StNF-Y基因在非生物逆境中的作用与功能,在马铃薯抗青枯病基因型ED13中克隆了1个新的NF-YC转录因子(StNF-Y),并对其进行生物信息学分析,预测其生物学功能。结果表明,StNF-Y基因的全长cDNA为1 132 bp,其开放阅读框为693 bp,编码230个氨基酸,氨基酸分子式为C_(1112)H_(1735)N_(317)O_(339)S_(10),等电点为5.37,含有13个磷酸化位点;StNF-Y蛋白分布在胞质中,无信号肽,属于亲水性蛋白,含有高度保守的结构域,属于BUR6 superfamily家族;二级结构显示,StNF-Y蛋白主要由45.65%的α-螺旋、5.65%的延伸链、45.22%的无规则卷曲和3.48%的β-转角组成。系统进化分析结果显示,马铃薯StNF-Y序列与番茄、辣椒和烟草NF-YC序列亲缘关系较近。此外,StNF-Y在多种植物胁迫中起作用。因此,推测StNF-Y可能参与马铃薯相关胁迫抗性的调节。研究结果可为以后进一步深入研究马铃薯StNF-Y的功能以及对逆境胁迫的抵抗机制提供理论依据。
To study the role and function of potato StNF-Y gene under abiotic stress,a new NF-YC transcription factor(StNF-Y)was cloned from potato resistant to bacterial wilt genotype ED13. Bioinformatics analysis and biological function prediction showed that the full-length cDNA of StNF-Y was 1 132 bp,with an open reading frame of 693 bp,encoding 230 amino acids,with a formula of C_(1112)H_(1735)N_(317)O_(339)S_(10) and isoelectric point of 5.37. It contained 13 phosphorylation sites,and StNF-Y protein was distributed in the cytoplasm,without signal peptide.It was a hydrophilic protein. StNF-Y protein contained a highly conserved domain,existed in the BUR6 superfamily. Secondary structure showed that the protein consisted mainly of 45.65% α-helix,5.65% extended chain,45.22% random coil and 3.48% β-turn. Phylogenetic analysis showed that the StNF-Y sequence of potato was closely related to the NF-YC sequences of tomato,pepper and tobacco. In addition,StNF-Y played a certain role in a variety of plant stresses,so it was speculated that StNF-Y might be involved in the regulation of potato-related stress resistance. The results would provide a theoretical basis for further research on the function of potato StNF-Y and its resistance mechanism to stress.
To study the role and function of potato StNF-Y gene under abiotic stress,a new NF-YC transcription factor(StNF-Y)was cloned from potato resistant to bacterial wilt genotype ED13. Bioinformatics analysis and biological function prediction showed that the full-length cDNA of StNF-Y was 1 132 bp,with an open reading frame of 693 bp,encoding 230 amino acids,with a formula of C_(1112)H_(1735)N_(317)O_(339)S_(10) and isoelectric point of 5.37. It contained 13 phosphorylation sites,and StNF-Y protein was distributed in the cytoplasm,without signal peptide.It was a hydrophilic protein. StNF-Y protein contained a highly conserved domain,existed in the BUR6 superfamily. Secondary structure showed that the protein consisted mainly of 45.65% α-helix,5.65% extended chain,45.22% random coil and 3.48% β-turn. Phylogenetic analysis showed that the StNF-Y sequence of potato was closely related to the NF-YC sequences of tomato,pepper and tobacco. In addition,StNF-Y played a certain role in a variety of plant stresses,so it was speculated that StNF-Y might be involved in the regulation of potato-related stress resistance. The results would provide a theoretical basis for further research on the function of potato StNF-Y and its resistance mechanism to stress.
引文
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[21] Lingle S E,Dyer J M.Cloning and expression of sucrose synthase-1 cDNA from sugarcane [J].Journal of Plant Physiology,2001,158(1):129-131.doi:10.1078/0176-1617-00266.
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[23] 苏丽艳.番茄SlETR6基因的克隆及非生物胁迫下的表达分析[J].华北农学报,2019,34(1):19-25.doi:10.7668/hbnxb.201751117.Su L Y.Cloning and expression analysis of SlETR6 gene in tomato under abiotic stress [J].Acta Agriculturae Boreali-Sinica,2019,34(1):19-25.
[24] 梁倩,李璐,安茜,周雅莉,王计平.紫苏脂肪酸去饱和酶基因PfFAD2的生物信息学及表达特性分析 [J].山西农业科学,2018,46(3):316-319.doi:10.3969/j.issn.1002-2481.2018.03.02.Liang Q,Li L,An Q,Zhou Y L,Wang J P.Bioinformatics and expression analysis of perilla fatty acid desaturase gene PfFAD2 [J].Journal of Shanxi Agricultural Sciences,2018,46(3):316-319.
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