瓜蒌子专属性成分的含量测定及其指纹图谱的研究
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摘要
目的:通过对10个产地的瓜蒌子HPLC指纹图谱的建立,以及栝楼仁三醇和栝楼仁二醇的含量测定,从而系统地建立瓜蒌子的质量评价体系。方法:采用高效液相色谱法,测定10个产地的瓜蒌子中栝楼仁三醇和栝楼仁二醇的含量;采用高效液相色谱法,以栝楼仁三醇为参考,建立瓜蒌子的HPLC指纹图谱。结果:在10个产地中,栝楼仁三醇的含量差异不大:其中安国的栝楼仁三醇含量最高,为1.455 mg/g,河南新乡的栝楼仁三醇含量最低,为0.947 9 mg/g;栝楼仁二醇的含量差异较大:其中定州的栝楼仁二醇含量最高,为0.096 70 mg/g,山东的栝楼仁二醇含量最低,为0.022 60 mg/g。通过建立10个产地瓜蒌子的HPLC指纹图谱,共确立了13个色谱共有峰,其中13号峰为对照品栝楼仁三醇的色谱峰。各个产地瓜蒌子HPLC指纹图谱的相似度均大于0.941。结论:栝楼仁三醇和栝楼仁二醇作为瓜蒌子的专属性成分,两者可作为瓜蒌子在质量定量控制方面的两个重要指标。建立的瓜蒌子HPLC指纹图谱,可为瓜蒌子在定性鉴别方面提供科学依据。
Objective:The quality evaluation system of in seeds of Fructus Trichosanthis was established through the establishment of HPLC fingerprint of in seeds of Fructus Trichosanthis in 10 producing areas and the determination of 3,29-Dibenzoyl rarounitriol and Karounidiol. Methods:HPLC method was used to determine the contents of 3,29-Dibenzoyl rarounitriol and Karounidiol in 10 producing areas of in seeds of Fructus Trichosanthis. HPLC fingerprint of the seeds of Fructus Trichosanthes was established by HPLC with 3,29-Dibenzoyl rarounitriol as reference substance. Results:In 10 producing areas, the contents of 3,29-Dibenzoyl rarounitriol and Karounidiol were not different. The highest content of 3,29-Dibenzoyl rarounitriol was 1.455 mg/g in Hebei Anguo and the lowest content of 3,29-Dibenzoyl rarounitriol was 0.947 9 mg/g in Henan Xinxiang. The highest content of Karounidiol in Hebei Dingzhou was 0.096 70 mg/g, and the lowest in the Karounidiol in Shandong was 0.022 60 mg/g. A total of 13 chromatographic peaks were established through the establishment of the HPLC fingerprint in seeds of Fructus Trichosanthes of 10 producing areas. Among them, the peak of number 13 was the chromatographic peak of the 3,29-Dibenzoyl rarounitriol. The similarity of HPLC fingerprints in seeds of Fructus Trichosanthis from various habitats was greater than 0.941. Conclusion: 3,29-Dibenzoyl rarounitriol and Karounidiol as special contents in seeds of Fructus Trichosanthis that can be used as two important indicators in the quantitative control in seeds of Fructus Trichosanthis. The HPLC fingerprintin seeds of Fructus Trichosanthis has high similarity and stable chromatographic peak, which meets the basic requirements of fingerprint. It can provide a scientific basis for the qualitative identification in seeds of Fructus Trichosanthis.
Objective:The quality evaluation system of in seeds of Fructus Trichosanthis was established through the establishment of HPLC fingerprint of in seeds of Fructus Trichosanthis in 10 producing areas and the determination of 3,29-Dibenzoyl rarounitriol and Karounidiol. Methods:HPLC method was used to determine the contents of 3,29-Dibenzoyl rarounitriol and Karounidiol in 10 producing areas of in seeds of Fructus Trichosanthis. HPLC fingerprint of the seeds of Fructus Trichosanthes was established by HPLC with 3,29-Dibenzoyl rarounitriol as reference substance. Results:In 10 producing areas, the contents of 3,29-Dibenzoyl rarounitriol and Karounidiol were not different. The highest content of 3,29-Dibenzoyl rarounitriol was 1.455 mg/g in Hebei Anguo and the lowest content of 3,29-Dibenzoyl rarounitriol was 0.947 9 mg/g in Henan Xinxiang. The highest content of Karounidiol in Hebei Dingzhou was 0.096 70 mg/g, and the lowest in the Karounidiol in Shandong was 0.022 60 mg/g. A total of 13 chromatographic peaks were established through the establishment of the HPLC fingerprint in seeds of Fructus Trichosanthes of 10 producing areas. Among them, the peak of number 13 was the chromatographic peak of the 3,29-Dibenzoyl rarounitriol. The similarity of HPLC fingerprints in seeds of Fructus Trichosanthis from various habitats was greater than 0.941. Conclusion: 3,29-Dibenzoyl rarounitriol and Karounidiol as special contents in seeds of Fructus Trichosanthis that can be used as two important indicators in the quantitative control in seeds of Fructus Trichosanthis. The HPLC fingerprintin seeds of Fructus Trichosanthis has high similarity and stable chromatographic peak, which meets the basic requirements of fingerprint. It can provide a scientific basis for the qualitative identification in seeds of Fructus Trichosanthis.
引文
[1] 国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2015:113.
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[4] 金情政,李钦,赵吟.瓜蒌子油对糖尿病小鼠降血糖作用的研究[J].药学实践杂志,2015,33(4):324-327.
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[9] 王玲娜,于京平,张永清.栝楼化学成分研究概述[J].环球中医药,2014,7(1):72-76.
[10] 刘明,李慧,李陈跟,等.瓜蒌化学成分及药用价值的研究综述[J].阜阳师范学院学报(自然科学版),2018,35(1):23-26.
[11] 徐何方,杨颂,李向日,等.瓜蒌(瓜蒌皮、瓜蒌子)炮制、化学成分和药理作用的研究进展[C]//全国中药炮制学术年会暨中药饮片创新发展论坛及协同创新联盟会议会议讲义.中华中医药学会中药炮制分会、中国医药物资协会中药饮片及生产设备协同创新联盟、中国医药保健品进出口商会中药饮片分会,2014:1.
[12] 张荣超,辛杰,郭庆梅,等.不同产区瓜蒌子有效成分含量测定[J].中国实验方剂学杂志,2013,19(18):71-73.
[13] 程雪梅,吴弢,侴桂新,等.RP-HPLC法测定瓜蒌子中3,29-二苯甲酰基栝楼仁三醇含量[J].药物分析杂志,2005,25(4):377-380.
[14] 谭翔,邹顺,薛琰,等.黔产瓜蒌中3,29-二苯甲酰基栝楼仁三醇的含量测定[J].贵阳中医学院学报,2014,36(3):14-15.
[15] 刘金娜,谢晓亮,杨太新,等.果实熟度及加工方式对瓜蒌子中3,29-二苯甲酰基栝楼仁三醇的影响[J].中药材,2014,37(4):581-583.
[16] 马跃平,李国玉,王金辉.HPLC-UV法同时测定瓜蒌仁及瓜蒌霜中4种三萜化合物的含量[C].中华中医药学会中药炮制分会.中华中医药学会中药炮制分会2011年学术年会论文集.中华中医药学会中药炮制分会,2011:3.
[17] 修彦凤,程雪梅,刘蕾,等.不同瓜蒌子饮片的成分比较[J].中草药,2005(1):33-35.
[18] 刘金娜.不同居群栝楼的农艺学特征比较及化学成分分析[D].石家庄:河北农业大学,2014.
[19] 韩琳娜.瓜蒌子有效成分提取方法研究进展[J].药学研究,2013,32(5):289-291.
[20] 张荣超,辛杰,王真真,等.瓜蒌子有效提取物质研究进展[J].辽宁中医杂志,2013,40(10):2157-2159.
[21] 巢志茂,唐春风,张贵峰,等.瓜蒌子的TLC鉴别和HPLC含量测定[J].中国实验方剂学杂志,2012,18(17):86-88.
[22] 唐春风.瓜蒌子的化学成分和定性定量研究[D].北京:中国协和医科大学,2005.
[23] 巢志茂,何波.双边栝楼种子中不皂化类脂的化学成分研究[J].中国药学杂志,2001(3):13-16.
[24] 巢志茂,何波,张颖,等.栝楼种子中不皂化类脂的化学成分研究[J].中国药学杂志,2000(11):13-16.
[25] 鄢海燕,邹纯才,汪小燕,等.瓜蒌及其炮制品的高效液相色谱指纹特征研究[J].中药材,2015,38(1):58-61.
[26] 郝变,潘丽丽,袁少雄,等.不同品种、产地瓜蒌皮中游离氨基酸类成分指纹图谱研究[J].中医药学报,2015,43(2):14-18.
[27] 史国玉,刘伟,耿岩玲,等.瓜蒌皮药材的HPLC指纹图谱[J].中国实验方剂学杂志,2014,20(1):73-76.
[28] 孙文,巢志茂,王淳,等.瓜蒌饮片高效液相色谱指纹图谱研究[J].中南药学,2013,11(10):757-760.
[29] 邹纯才,徐启祥,鄢海燕,等.不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹特征相似性研究[J].国际药学研究杂志,2017,44(3):278-281.
[30] 邹纯才,宗倩妮,鄢海燕,等.瓜蒌及其蒸制品HPLC指纹图谱及6种成分的定量分析[J].中国药学杂志,2017,52(7):597-601.
[31] 宋艳梅,刘亚鲁,李峰.山东仁瓜蒌的HPLC指纹图谱研究[J].西部中医药,2016,29(8):24-28.
[32] 宋艳梅.瓜蒌多元多息指纹图谱的鉴别研究及其品质相关数学模型的构建[D].济南:山东中医药大学,2011.
[33] 宋九华,张艳明,成英.不同产地金银花药材HPLC指纹图谱的研究[J].化学研究与应用,2018(5):823-827.
[34] 高婧,王领弟,李艳荣,等.不同产地山楂叶HPLC指纹图谱比较分析[J].安徽医药,2018(1):38-42.
[2] 林树花,李高阳,韩晓磊.栝楼籽的化学成分研究进展[J].农产品加工(创新版),2012(8):73-77.
[3] 万丽娟,卢金清,许俊洁,等.瓜蒌子化学成分和药理作用的研究进展[J].中国药房,2015,26(31):4440-4443.
[4] 金情政,李钦,赵吟.瓜蒌子油对糖尿病小鼠降血糖作用的研究[J].药学实践杂志,2015,33(4):324-327.
[5] 王梦晗,乔勇进,陆凤英,等.瓜蒌的功效成分及开发利用研究概述[J].保鲜与加工,2015,15(3):68-71,75.
[6] 孙娟,孟冰雪,赵启韬.瓜蒌药理作用的物质基础研究概况[J].山东中医杂志,2012,31(6):461-463.
[7] 屠婕红,余菁,陈伟光.瓜蒌的化学成分和药理作用研究概况[J].中国药师,2004(7):562-563,572
[8] 滕勇荣,张永清.瓜蒌化学成分研究进展[J].山东中医药大学学报,2011,35(1):85-86.
[9] 王玲娜,于京平,张永清.栝楼化学成分研究概述[J].环球中医药,2014,7(1):72-76.
[10] 刘明,李慧,李陈跟,等.瓜蒌化学成分及药用价值的研究综述[J].阜阳师范学院学报(自然科学版),2018,35(1):23-26.
[11] 徐何方,杨颂,李向日,等.瓜蒌(瓜蒌皮、瓜蒌子)炮制、化学成分和药理作用的研究进展[C]//全国中药炮制学术年会暨中药饮片创新发展论坛及协同创新联盟会议会议讲义.中华中医药学会中药炮制分会、中国医药物资协会中药饮片及生产设备协同创新联盟、中国医药保健品进出口商会中药饮片分会,2014:1.
[12] 张荣超,辛杰,郭庆梅,等.不同产区瓜蒌子有效成分含量测定[J].中国实验方剂学杂志,2013,19(18):71-73.
[13] 程雪梅,吴弢,侴桂新,等.RP-HPLC法测定瓜蒌子中3,29-二苯甲酰基栝楼仁三醇含量[J].药物分析杂志,2005,25(4):377-380.
[14] 谭翔,邹顺,薛琰,等.黔产瓜蒌中3,29-二苯甲酰基栝楼仁三醇的含量测定[J].贵阳中医学院学报,2014,36(3):14-15.
[15] 刘金娜,谢晓亮,杨太新,等.果实熟度及加工方式对瓜蒌子中3,29-二苯甲酰基栝楼仁三醇的影响[J].中药材,2014,37(4):581-583.
[16] 马跃平,李国玉,王金辉.HPLC-UV法同时测定瓜蒌仁及瓜蒌霜中4种三萜化合物的含量[C].中华中医药学会中药炮制分会.中华中医药学会中药炮制分会2011年学术年会论文集.中华中医药学会中药炮制分会,2011:3.
[17] 修彦凤,程雪梅,刘蕾,等.不同瓜蒌子饮片的成分比较[J].中草药,2005(1):33-35.
[18] 刘金娜.不同居群栝楼的农艺学特征比较及化学成分分析[D].石家庄:河北农业大学,2014.
[19] 韩琳娜.瓜蒌子有效成分提取方法研究进展[J].药学研究,2013,32(5):289-291.
[20] 张荣超,辛杰,王真真,等.瓜蒌子有效提取物质研究进展[J].辽宁中医杂志,2013,40(10):2157-2159.
[21] 巢志茂,唐春风,张贵峰,等.瓜蒌子的TLC鉴别和HPLC含量测定[J].中国实验方剂学杂志,2012,18(17):86-88.
[22] 唐春风.瓜蒌子的化学成分和定性定量研究[D].北京:中国协和医科大学,2005.
[23] 巢志茂,何波.双边栝楼种子中不皂化类脂的化学成分研究[J].中国药学杂志,2001(3):13-16.
[24] 巢志茂,何波,张颖,等.栝楼种子中不皂化类脂的化学成分研究[J].中国药学杂志,2000(11):13-16.
[25] 鄢海燕,邹纯才,汪小燕,等.瓜蒌及其炮制品的高效液相色谱指纹特征研究[J].中药材,2015,38(1):58-61.
[26] 郝变,潘丽丽,袁少雄,等.不同品种、产地瓜蒌皮中游离氨基酸类成分指纹图谱研究[J].中医药学报,2015,43(2):14-18.
[27] 史国玉,刘伟,耿岩玲,等.瓜蒌皮药材的HPLC指纹图谱[J].中国实验方剂学杂志,2014,20(1):73-76.
[28] 孙文,巢志茂,王淳,等.瓜蒌饮片高效液相色谱指纹图谱研究[J].中南药学,2013,11(10):757-760.
[29] 邹纯才,徐启祥,鄢海燕,等.不同配伍比例的瓜蒌皮-瓜蒌子与瓜蒌的化学指纹特征相似性研究[J].国际药学研究杂志,2017,44(3):278-281.
[30] 邹纯才,宗倩妮,鄢海燕,等.瓜蒌及其蒸制品HPLC指纹图谱及6种成分的定量分析[J].中国药学杂志,2017,52(7):597-601.
[31] 宋艳梅,刘亚鲁,李峰.山东仁瓜蒌的HPLC指纹图谱研究[J].西部中医药,2016,29(8):24-28.
[32] 宋艳梅.瓜蒌多元多息指纹图谱的鉴别研究及其品质相关数学模型的构建[D].济南:山东中医药大学,2011.
[33] 宋九华,张艳明,成英.不同产地金银花药材HPLC指纹图谱的研究[J].化学研究与应用,2018(5):823-827.
[34] 高婧,王领弟,李艳荣,等.不同产地山楂叶HPLC指纹图谱比较分析[J].安徽医药,2018(1):38-42.