水稻α-NAC基因家族的克隆及生物信息学分析
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摘要
新生多肽结合复合体(Nascent polypeptide-associated complex,NAC)是由α和β两个亚基组成的异二聚体复合物。水稻新生多肽结合复合体α链基因家族共有3个基因,分别位于1、3号和5号染色体上。本研究根据公布的水稻基因组序列,通过RT-PCR方法克隆了粳稻品种中花11号中α-NAC家族的3个基因。经生物信息学分析发现,水稻α-NAC基因家族的氨基酸序列比较保守,包含NAC和UBA两个结构域,与玉米NAC基因家族的亲缘关系最近。Os1gNAC和Os3gNAC、Os5gNAC的相似性分别为67.4%、81.8%,Os3gNAC和Os5gNAC的相似性为69.5%。本研究结果为Osα-NAC蛋白纯化及蛋白结构生物学研究提供参考,为今后Osα-NAC基因家族的生物学功能研究奠定基础。
Nascent polypeptide- associated complex(NAC) is a heterodimer consisting of α and β subunits. There are three members of α-NAC in rice genome,which are localized in chromosome 1,3 and 5 respectively. The full open reading frames of the three α-NAC genes were cloned by RT- PCR from Oryza sativa cv. Zhonghua 11. Further bioinformatics analysis revealed that the amino acid sequence of Osα-NAC was conserved inNAC and UBA domains,and showed the highest similarity to ZmNACs. Os1gNAC shared67. 4% and 81. 8% homology with Os3gNAC and Os5gNAC respectively,whereas Os3gNAC and Os5gNAC shared 69. 5% homology. These results would provide references for protein purification and structural biological study,and lay a foundation for functional analysis of Osα-NAC.
Nascent polypeptide- associated complex(NAC) is a heterodimer consisting of α and β subunits. There are three members of α-NAC in rice genome,which are localized in chromosome 1,3 and 5 respectively. The full open reading frames of the three α-NAC genes were cloned by RT- PCR from Oryza sativa cv. Zhonghua 11. Further bioinformatics analysis revealed that the amino acid sequence of Osα-NAC was conserved inNAC and UBA domains,and showed the highest similarity to ZmNACs. Os1gNAC shared67. 4% and 81. 8% homology with Os3gNAC and Os5gNAC respectively,whereas Os3gNAC and Os5gNAC shared 69. 5% homology. These results would provide references for protein purification and structural biological study,and lay a foundation for functional analysis of Osα-NAC.
引文
[1]Wiedmann B,Sakai H,Davis T A,et al.A protein complex required for signal sequence specific sorting and translocation[J].Nature,1994,370:434-440.
[2]Rospert S,Dubaquie Y,Gautschi M.Nascent polypeptide associated complex[J].Cell Mol.Life Sci.,2002,59:1632-1639.
[3]Yotov W V,Moreau A,St-Arnaud R.The alpha chain of the nascent polypeptide associated complex functions as a transcriptional coactivator[J].Mol.Cell Biol.,1998,18(3):1303-1311.
[4]Moreau A,Yotov W V,Glorieux F H,et al.Bone specific expression of the alpha chain of the nascent polypeptide associated complex,a coactivator potentiating c-Jun mediated transcription[J].Mol.Cell Biol.,1998,18(3):1312-1321.
[5]Quelo I,Gauthier C,Hannigan G E,et al.Integrin linked kinase regulates the nuclear entry of the c-Jun coactivator alpha NAC and its coactivation potency[J].J.Biol.Chem.,2004,279:43893-43899.
[6]Huh S U,Kim K J,Paek K H.Capsicum annuum basic transcription factor 3(Ca Btf3)regulates transcription of pathogenesis related genes during hypersensitive response upon Tobacco mosaic virus infection[J].Biochem.Biophys.Res.Commun.,2012,417:910-917.
[7]Karan R,Subudhi P K.Overexpression of a nascent polypeptide associated complex gene(SaβNAC)of Spartina alterniflora improves tolerance to salinity and drought in transgenic Arabidopsis[J].Biochem.Biophys.Res.Commun.,2012,424:747-752.
[8]Kang G,Ma H,Liu G,et al.Silencing of Ta BTF3 gene impairs tolerance to freezing and drought stresses in wheat[J].Mol.Genet.Genomics,2013,288:591-599.
[9]Wang W,Xu M,Wang Y,et al.Basal transcription factor 3plays an important role in seed germination and seedling growth of rice[J].Biomed.Res.Int.,2014,54:957-964.
[10]Wang Y,Zhang X,Lu S,et al.Inhibition of a basal transcription factor 3-like gene Osj10g BTF3 in rice results in significant plant miniaturization and typical pollen abortion[J].Plant Cell Physiol.,2012,53:2073-2089.
[11]Preissler S,Deuerling E.Ribosome-associated chaperones as key players in proteostasis[J].Trends Biochem.Sci.,2012,37:274-283.
[12]李广旭,陈华民,何晨阳,等.Os BTF3过表达转基因水稻株系的创制及其分子鉴定[J].生物技术进展,2012,2(1):39-43.
[13]李广旭,陈华民,何晨阳,等.Os BTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定[J].中国水稻科学,2012,26(1):5-8.
[14]李广旭,陈华民,何晨阳,等.Os BTF3基因在水稻光合作用和生长发育中的功能[J].作物学报,2012,38(5):904-908.
[15]李广旭,陈华民,何晨阳,等.Os BTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析[J].植物病理学报,2012,42(2):169-175.
[2]Rospert S,Dubaquie Y,Gautschi M.Nascent polypeptide associated complex[J].Cell Mol.Life Sci.,2002,59:1632-1639.
[3]Yotov W V,Moreau A,St-Arnaud R.The alpha chain of the nascent polypeptide associated complex functions as a transcriptional coactivator[J].Mol.Cell Biol.,1998,18(3):1303-1311.
[4]Moreau A,Yotov W V,Glorieux F H,et al.Bone specific expression of the alpha chain of the nascent polypeptide associated complex,a coactivator potentiating c-Jun mediated transcription[J].Mol.Cell Biol.,1998,18(3):1312-1321.
[5]Quelo I,Gauthier C,Hannigan G E,et al.Integrin linked kinase regulates the nuclear entry of the c-Jun coactivator alpha NAC and its coactivation potency[J].J.Biol.Chem.,2004,279:43893-43899.
[6]Huh S U,Kim K J,Paek K H.Capsicum annuum basic transcription factor 3(Ca Btf3)regulates transcription of pathogenesis related genes during hypersensitive response upon Tobacco mosaic virus infection[J].Biochem.Biophys.Res.Commun.,2012,417:910-917.
[7]Karan R,Subudhi P K.Overexpression of a nascent polypeptide associated complex gene(SaβNAC)of Spartina alterniflora improves tolerance to salinity and drought in transgenic Arabidopsis[J].Biochem.Biophys.Res.Commun.,2012,424:747-752.
[8]Kang G,Ma H,Liu G,et al.Silencing of Ta BTF3 gene impairs tolerance to freezing and drought stresses in wheat[J].Mol.Genet.Genomics,2013,288:591-599.
[9]Wang W,Xu M,Wang Y,et al.Basal transcription factor 3plays an important role in seed germination and seedling growth of rice[J].Biomed.Res.Int.,2014,54:957-964.
[10]Wang Y,Zhang X,Lu S,et al.Inhibition of a basal transcription factor 3-like gene Osj10g BTF3 in rice results in significant plant miniaturization and typical pollen abortion[J].Plant Cell Physiol.,2012,53:2073-2089.
[11]Preissler S,Deuerling E.Ribosome-associated chaperones as key players in proteostasis[J].Trends Biochem.Sci.,2012,37:274-283.
[12]李广旭,陈华民,何晨阳,等.Os BTF3过表达转基因水稻株系的创制及其分子鉴定[J].生物技术进展,2012,2(1):39-43.
[13]李广旭,陈华民,何晨阳,等.Os BTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定[J].中国水稻科学,2012,26(1):5-8.
[14]李广旭,陈华民,何晨阳,等.Os BTF3基因在水稻光合作用和生长发育中的功能[J].作物学报,2012,38(5):904-908.
[15]李广旭,陈华民,何晨阳,等.Os BTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析[J].植物病理学报,2012,42(2):169-175.