丁香酚在罗非鱼血浆中药代动力学研究
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摘要
目的在理想麻醉浓度条件下,研究丁香酚在罗非鱼血浆中药代动力学。方法选取体重为(250±10)g的罗非鱼放入丁香酚理想麻醉浓度溶液中药浴,待完全麻醉后,放入清水中复苏,幵在0～18 h间隔取样,采用液相色谱串联质谱法检测血浆中丁香酚含量, DAS2.0软件计算血浆中丁香酚药代动力学参数。结果药代动力学参数显示,丁香酚在罗非鱼血浆中最高血药浓度C_(max)为4.81mg/L,消除半衰期t_(1/2β)为2.06h,平均滞留时间(mean residence time, MRT)为3.34 h,药时曲线下面积(area under the curve, AUC)为14.55 mg/(L·h)。结论丁香酚在罗非鱼血浆中半衰期短,消除速率较快,药代参数符合二室模型。
Objective To investigate the pharmacokinetics of eugenol in tilapia plasma under the optimal level of anesthesia. Methods The tilapia with body weight of(250±10) g were bathed in eugenol solution for anesthesia,after complete anesthesia, the tilapia were put into water to recovery. The plasma of tilapia collected at 0～18 h was detected by liquid chromatography-tandem mass spectrometry. The pharmacokinetic parameters of eugenol in plasma were calculated by DAS2.0 software. Results The results showed that the peak concentrations(C_(max)) in tilapia plasma was 4.81 mg/L and the elimination half-life(t_(1/2β)) was 2.06 h. In addition, the mean residence time(MRT0-∞)was 3.34 h and the area under the concentration-time curve(AUC0-∞) was 14.55 mg/(L·h). Conclusion Eugenol has a short half-life and its elimination is relatively fast in tilapia plasma, which is the pharmacokinetic parameters fitted to two-compartment model.
Objective To investigate the pharmacokinetics of eugenol in tilapia plasma under the optimal level of anesthesia. Methods The tilapia with body weight of(250±10) g were bathed in eugenol solution for anesthesia,after complete anesthesia, the tilapia were put into water to recovery. The plasma of tilapia collected at 0～18 h was detected by liquid chromatography-tandem mass spectrometry. The pharmacokinetic parameters of eugenol in plasma were calculated by DAS2.0 software. Results The results showed that the peak concentrations(C_(max)) in tilapia plasma was 4.81 mg/L and the elimination half-life(t_(1/2β)) was 2.06 h. In addition, the mean residence time(MRT0-∞)was 3.34 h and the area under the concentration-time curve(AUC0-∞) was 14.55 mg/(L·h). Conclusion Eugenol has a short half-life and its elimination is relatively fast in tilapia plasma, which is the pharmacokinetic parameters fitted to two-compartment model.
引文
[1]杨移斌,夏永涛,艾晓辉,等.丁香油对俄罗斯鲟的麻醉效果[J].中国渔业质量与标准,2016,6(1):17-21.Yang YB,Xia YT,Ai XH,et al.Anaesthetic effects of eugenol on Acipenser gueldenstaedti[J].Chin Fish Qual Standard,2016,6(1):17-21.
[2]梁政远,安丼娜,繆凌鸿,等.丁香油对鲤鱼和罗非鱼的麻醉作用[J].中国水产学杂志,2009,22(1):42-46.Liang ZY,An LN,Miao LH,et al.The anaesthesia effect of clove oil on common carp(Cyprinus carpio Linnaeus)and tilapia(Oreochromis spp)[J].Chin J Fish,2009,22(1):42-46.
[3]刘长琳,李继强,陈四清,等.丁香酚麻醉半滑舌鳎成鱼的试验研究[J].海洋水产研究,2007,28(3):50-56.Liu CL,Li JQ,Chen SQ,et al.Study on the anaesthetic effects of clove oil on adult Cynoglossus Semillaevis Günther[J].Marine Fish Res,2007,28(3):50-56.
[4]李靖,刘宝良,王顺奎,等.丁香酚对大西洋鲑麻醉效果的实验研究[J].海洋科学进展,2015,33(1):92-99.Li J,Liu BL,Wang SK,et al.The anaesthesia effect of eugenol on Salmo salar[J].Adv Marine Sci,2015,33(1):92-99.
[5]张志明,胡盼,姜志强,等.丁香酚对锦鲤麻醉效果的研究[J].水产科学,2014,33(1):40-45.Zhang ZM,Hu P,Jiang ZQ,et al.The anaesthesia effect of clove oil on Koi Carp(Cyprinus carpio)[J].Fish Sci,2014,33(1):40-45.
[6]斱晓磊,柯常亮,刘奇,等.水产品中丁香酚的人体健康风险分析[J].水产科学,2018,37(1):140-144.Fang XL,Ke CL,Liu Q,et al.Human health risk evaluation of eugenol residues in fish[J].Fish Sci,2018,37(1):140-144.
[7]吕海燕,王群,刘欢,等.鱼用麻醉剂安全性研究进展[J].中国渔业质量与标准,2013,3(2):24-28.Lv HY,Wang Q,Liu H,et al.The research progress of anesthetics safety in fish[J].Chin Fish Qual Standards,2013,3(2):24-28.
[8]李晋成,刘欢,王群,等.水产品中丁香酚的残留风险评估研究进展[J].食品安全质量检测学报,2014,5(11):3598-3602.Li JC,Liu H,Wang Q,et al.Progress in risk assessment of eugenol residues in aquatic products[J].J Food Saf Qual,2014,5(11):3598-3602.
[9]金元,何雅静,程波,等.丁香酚类麻醉剂的安全性探讨[J].食品安全质量检测学报,2017,8(1):33-40.Jin Y,He YJ,Cheng B,et al.Research on safety of eugenol derivatives anesthetics[J].J Food Saf Qual,2017,8(1):33-40.
[10]Marking LL,Meyer FP.Are better anesthetics needed in fisheries[J].Fisheries,1985,10(6):2-5.
[11]郭丰红,汪之和.MS-222对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009,(7):150-153.Guo FH,Wang ZH.Anesthetic effect of MS-222 on adult Siniperca chuatsi[J].Hunan Agric Sci,2009,(7):150-153.
[12]罗晶晶,韩典峰,孙玉增,等.丁香酚对罗非鱼的麻醉效果[J].河北渔业,2018,(3):5-8.Luo JJ,Han DF,Sun YZ,et al.The effects of eugenol on the anaesthetic of tilapia[J].Hebei Yuye,2018,(3):5-8.
[13]芦智远.SPE-UPLC-MS/MS测定鱼肉中丁香酚的残留量[D].杨凌:西北农林科技大学,2016.Lu ZY.Determination of eugenol residue in fish meat by SPE-UPLC-MS/MS[D].Yangling:Northwest A&F University,2016.
[14]Guénette SA,Uhland FC,Hélie P,et al.Pharmacokinetics of eugenol in rainbow trout(Oncorhynchusmykiss)[J].Aquaculture,2007,266(1-4):262-265.
[15]斱晓磊.青石斑鱼中丁香酚的残留消除特征研究[D].舟山:浙江海洋大学,2017.Fang XL.Depletion of eugenol in the fish fillet of Yellow Grouper(Epinephelus awoara)[D].Zhoushan:Zhejiang Ocean University,2017.
[2]梁政远,安丼娜,繆凌鸿,等.丁香油对鲤鱼和罗非鱼的麻醉作用[J].中国水产学杂志,2009,22(1):42-46.Liang ZY,An LN,Miao LH,et al.The anaesthesia effect of clove oil on common carp(Cyprinus carpio Linnaeus)and tilapia(Oreochromis spp)[J].Chin J Fish,2009,22(1):42-46.
[3]刘长琳,李继强,陈四清,等.丁香酚麻醉半滑舌鳎成鱼的试验研究[J].海洋水产研究,2007,28(3):50-56.Liu CL,Li JQ,Chen SQ,et al.Study on the anaesthetic effects of clove oil on adult Cynoglossus Semillaevis Günther[J].Marine Fish Res,2007,28(3):50-56.
[4]李靖,刘宝良,王顺奎,等.丁香酚对大西洋鲑麻醉效果的实验研究[J].海洋科学进展,2015,33(1):92-99.Li J,Liu BL,Wang SK,et al.The anaesthesia effect of eugenol on Salmo salar[J].Adv Marine Sci,2015,33(1):92-99.
[5]张志明,胡盼,姜志强,等.丁香酚对锦鲤麻醉效果的研究[J].水产科学,2014,33(1):40-45.Zhang ZM,Hu P,Jiang ZQ,et al.The anaesthesia effect of clove oil on Koi Carp(Cyprinus carpio)[J].Fish Sci,2014,33(1):40-45.
[6]斱晓磊,柯常亮,刘奇,等.水产品中丁香酚的人体健康风险分析[J].水产科学,2018,37(1):140-144.Fang XL,Ke CL,Liu Q,et al.Human health risk evaluation of eugenol residues in fish[J].Fish Sci,2018,37(1):140-144.
[7]吕海燕,王群,刘欢,等.鱼用麻醉剂安全性研究进展[J].中国渔业质量与标准,2013,3(2):24-28.Lv HY,Wang Q,Liu H,et al.The research progress of anesthetics safety in fish[J].Chin Fish Qual Standards,2013,3(2):24-28.
[8]李晋成,刘欢,王群,等.水产品中丁香酚的残留风险评估研究进展[J].食品安全质量检测学报,2014,5(11):3598-3602.Li JC,Liu H,Wang Q,et al.Progress in risk assessment of eugenol residues in aquatic products[J].J Food Saf Qual,2014,5(11):3598-3602.
[9]金元,何雅静,程波,等.丁香酚类麻醉剂的安全性探讨[J].食品安全质量检测学报,2017,8(1):33-40.Jin Y,He YJ,Cheng B,et al.Research on safety of eugenol derivatives anesthetics[J].J Food Saf Qual,2017,8(1):33-40.
[10]Marking LL,Meyer FP.Are better anesthetics needed in fisheries[J].Fisheries,1985,10(6):2-5.
[11]郭丰红,汪之和.MS-222对鳜鱼成鱼麻醉效果的研究[J].湖南农业科学,2009,(7):150-153.Guo FH,Wang ZH.Anesthetic effect of MS-222 on adult Siniperca chuatsi[J].Hunan Agric Sci,2009,(7):150-153.
[12]罗晶晶,韩典峰,孙玉增,等.丁香酚对罗非鱼的麻醉效果[J].河北渔业,2018,(3):5-8.Luo JJ,Han DF,Sun YZ,et al.The effects of eugenol on the anaesthetic of tilapia[J].Hebei Yuye,2018,(3):5-8.
[13]芦智远.SPE-UPLC-MS/MS测定鱼肉中丁香酚的残留量[D].杨凌:西北农林科技大学,2016.Lu ZY.Determination of eugenol residue in fish meat by SPE-UPLC-MS/MS[D].Yangling:Northwest A&F University,2016.
[14]Guénette SA,Uhland FC,Hélie P,et al.Pharmacokinetics of eugenol in rainbow trout(Oncorhynchusmykiss)[J].Aquaculture,2007,266(1-4):262-265.
[15]斱晓磊.青石斑鱼中丁香酚的残留消除特征研究[D].舟山:浙江海洋大学,2017.Fang XL.Depletion of eugenol in the fish fillet of Yellow Grouper(Epinephelus awoara)[D].Zhoushan:Zhejiang Ocean University,2017.