摘要
目的在理想麻醉浓度条件下,研究丁香酚在罗非鱼血浆中药代动力学。方法选取体重为(250±10)g的罗非鱼放入丁香酚理想麻醉浓度溶液中药浴,待完全麻醉后,放入清水中复苏,幵在0~18 h间隔取样,采用液相色谱串联质谱法检测血浆中丁香酚含量, DAS2.0软件计算血浆中丁香酚药代动力学参数。结果药代动力学参数显示,丁香酚在罗非鱼血浆中最高血药浓度C_(max)为4.81mg/L,消除半衰期t_(1/2β)为2.06h,平均滞留时间(mean residence time, MRT)为3.34 h,药时曲线下面积(area under the curve, AUC)为14.55 mg/(L·h)。结论丁香酚在罗非鱼血浆中半衰期短,消除速率较快,药代参数符合二室模型。
Objective To investigate the pharmacokinetics of eugenol in tilapia plasma under the optimal level of anesthesia. Methods The tilapia with body weight of(250±10) g were bathed in eugenol solution for anesthesia,after complete anesthesia, the tilapia were put into water to recovery. The plasma of tilapia collected at 0~18 h was detected by liquid chromatography-tandem mass spectrometry. The pharmacokinetic parameters of eugenol in plasma were calculated by DAS2.0 software. Results The results showed that the peak concentrations(C_(max)) in tilapia plasma was 4.81 mg/L and the elimination half-life(t_(1/2β)) was 2.06 h. In addition, the mean residence time(MRT0-∞)was 3.34 h and the area under the concentration-time curve(AUC0-∞) was 14.55 mg/(L·h). Conclusion Eugenol has a short half-life and its elimination is relatively fast in tilapia plasma, which is the pharmacokinetic parameters fitted to two-compartment model.
引文
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