HPLC法同时测定宝儿康散中9种成分
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摘要
目的建立HPLC法同时测定宝儿康散(太子参、白术、茯苓等)中太子参环肽B、白术内酯Ⅲ、白术内酯Ⅰ、去氢土莫酸、土莫酸、去氢茯苓酸、茯苓酸、β-细辛醚、α-细辛醚的含有量。方法该药物甲醇提取液的分析采用Agilent Eclipse XDB-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.05%磷酸,梯度洗脱;检测波长203、210、220、257 nm;体积流量0.9 mL/min;柱温35℃。结果 9种成分在各自范围内线性关系良好(r≥0.999 1),平均加样回收率96.94%～100.05%,RSD 0.59%～1.34%。结论该方法简便准确,重复性好,可用于宝儿康散的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of heterophyllin B, atracylenolide Ⅲ, atracylenolideⅠ, dehydrotumulosic acid, tumulosic acid, dehydropachymic acid, pachymic acid, β-asarone and α-asarone in Bao'erkang Powder(Pseudostellariae Radix, Atractylodis macrocephalae Rhizoma, Poria, etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Eclipse XDB-C_(18 )column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.05% phosphoric acid flowing at 0.9 mL/min in a gradient elution manner, and the detection wavelengths were set at 203, 210, 220, 257 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 1), whose average recoveries were 96.94%-100.05% with the RSDs of 0.59%-1.34%.CONCLUSION This simple, accurate and reproducible method can be used for the quality control of Bao'erkang Powder.
AIM To establish an HPLC method for the simultaneous content determination of heterophyllin B, atracylenolide Ⅲ, atracylenolideⅠ, dehydrotumulosic acid, tumulosic acid, dehydropachymic acid, pachymic acid, β-asarone and α-asarone in Bao'erkang Powder(Pseudostellariae Radix, Atractylodis macrocephalae Rhizoma, Poria, etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Eclipse XDB-C_(18 )column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.05% phosphoric acid flowing at 0.9 mL/min in a gradient elution manner, and the detection wavelengths were set at 203, 210, 220, 257 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 1), whose average recoveries were 96.94%-100.05% with the RSDs of 0.59%-1.34%.CONCLUSION This simple, accurate and reproducible method can be used for the quality control of Bao'erkang Powder.
引文
[1] 中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准(中药成方制剂第十册)[S].1995:96.
[2] 吴玉燕,张伟.宝儿康散治疗小儿急性腹泻病的疗效观察[J].中国社区医师(医学专业),2012,14(3):197.
[3] 张丽学.宝儿康散治疗脾虚湿困型小儿腹泻的临床疗效观察[J].中国中药杂志,2015,40(8):1605-1609.
[4] 钟成梁,胡思源,郭圣璇,等.宝儿康散剂量加倍治疗小儿慢性迁延性腹泻病脾虚湿困证评价其有效性和安全性的随机双盲、阳性药和原剂量平行对照、多中心临床试验[J].天津中医药,2018,35(5):324-328.
[5] 汪秀云,甄党霞.中药宝儿康散治疗秋季小儿腹泻120例疗效观察[J].内蒙古中医药,2011,30(15):52-53.
[6] 杨树英,帅普霞.宝儿康散治疗小儿营养性缺铁性贫血62例[J].中国药业,2012,21(4):80.
[7] 红霞,李喆.宝儿康散与思连康联用治疗婴幼儿非感染性腹泻158例报告[J].中国实用医药,2006,1(6):60-61.
[8] 池浩波,张雪原,林晓辉,等.宝儿康散质量标准修订研究[J].中国药品标准,2008,9(5):334-338.
[9] 周汉明,田贞东.宝儿康颗粒质量标准研究[J].湖北中医杂志,2013,35(1):74-76.
[10] 胡军林,顾承刚,吴宁.宝儿康颗粒的质量标准研究[J].中国医药指南,2014,12(16):94-96.
[11] 龙全江,王晓阁,徐雪琴.安徽与其它主产区地产太子参药材中太子参环肽B的含量比较研究[J].现代中药研究与实践,2016,30(3):55-57.
[12] 王晓阁,龙全江,赵悦,等.产地不同加工方法对太子参药材中太子参环肽B含量的影响[J].甘肃中医药大学学报,2016,33(3):45-49.
[13] 许祥君,陈敏,宗露,等.不同品规白术中白术内酯Ⅰ、Ⅲ的含量测定[J].中国医院药学杂志,2011,31(17):1421-1424.
[14] 吴慧,赵文龙,单国顺,等.HPLC波长切换法同时测定白术及其不同麸制品中白术内酯Ⅰ、Ⅱ、Ⅲ[J].中成药,2013,35(11):2484-2487.
[15] 张靓琦,贾英,罗洁,等.超高效液相色谱法同时测定茯苓中去氢土莫酸等6种活性成分的含量[J].中国药学杂志,2012,47(13):1080-1083.
[16] 车爽,李清,霍艳双,等.波长转换RP-HPLC法同时测定茯苓不同部位中5种三萜酸含量[J].药学学报,2010,45(4):494-497.
[17] 柳梦婷,方婧,吴宏伟,等.高效液相色谱法同时检测石菖蒲中β-细辛醚、α-细辛醚的含量[J].中国实验方剂学杂志,2014,20(19):75-78.
[18] 陈龙梗,杨青山,杨烁,等.高效液相色谱法测定安徽产石菖蒲中α-细辛醚、β-细辛醚的含量[J].甘肃中医药大学学报,2017,34(5):15-18.
[2] 吴玉燕,张伟.宝儿康散治疗小儿急性腹泻病的疗效观察[J].中国社区医师(医学专业),2012,14(3):197.
[3] 张丽学.宝儿康散治疗脾虚湿困型小儿腹泻的临床疗效观察[J].中国中药杂志,2015,40(8):1605-1609.
[4] 钟成梁,胡思源,郭圣璇,等.宝儿康散剂量加倍治疗小儿慢性迁延性腹泻病脾虚湿困证评价其有效性和安全性的随机双盲、阳性药和原剂量平行对照、多中心临床试验[J].天津中医药,2018,35(5):324-328.
[5] 汪秀云,甄党霞.中药宝儿康散治疗秋季小儿腹泻120例疗效观察[J].内蒙古中医药,2011,30(15):52-53.
[6] 杨树英,帅普霞.宝儿康散治疗小儿营养性缺铁性贫血62例[J].中国药业,2012,21(4):80.
[7] 红霞,李喆.宝儿康散与思连康联用治疗婴幼儿非感染性腹泻158例报告[J].中国实用医药,2006,1(6):60-61.
[8] 池浩波,张雪原,林晓辉,等.宝儿康散质量标准修订研究[J].中国药品标准,2008,9(5):334-338.
[9] 周汉明,田贞东.宝儿康颗粒质量标准研究[J].湖北中医杂志,2013,35(1):74-76.
[10] 胡军林,顾承刚,吴宁.宝儿康颗粒的质量标准研究[J].中国医药指南,2014,12(16):94-96.
[11] 龙全江,王晓阁,徐雪琴.安徽与其它主产区地产太子参药材中太子参环肽B的含量比较研究[J].现代中药研究与实践,2016,30(3):55-57.
[12] 王晓阁,龙全江,赵悦,等.产地不同加工方法对太子参药材中太子参环肽B含量的影响[J].甘肃中医药大学学报,2016,33(3):45-49.
[13] 许祥君,陈敏,宗露,等.不同品规白术中白术内酯Ⅰ、Ⅲ的含量测定[J].中国医院药学杂志,2011,31(17):1421-1424.
[14] 吴慧,赵文龙,单国顺,等.HPLC波长切换法同时测定白术及其不同麸制品中白术内酯Ⅰ、Ⅱ、Ⅲ[J].中成药,2013,35(11):2484-2487.
[15] 张靓琦,贾英,罗洁,等.超高效液相色谱法同时测定茯苓中去氢土莫酸等6种活性成分的含量[J].中国药学杂志,2012,47(13):1080-1083.
[16] 车爽,李清,霍艳双,等.波长转换RP-HPLC法同时测定茯苓不同部位中5种三萜酸含量[J].药学学报,2010,45(4):494-497.
[17] 柳梦婷,方婧,吴宏伟,等.高效液相色谱法同时检测石菖蒲中β-细辛醚、α-细辛醚的含量[J].中国实验方剂学杂志,2014,20(19):75-78.
[18] 陈龙梗,杨青山,杨烁,等.高效液相色谱法测定安徽产石菖蒲中α-细辛醚、β-细辛醚的含量[J].甘肃中医药大学学报,2017,34(5):15-18.