硫酸酯化修饰对白背三七多糖抗氧化性能的影响
|
摘要
采用浓硫酸法对白背三七多糖(Gynura davaricata(L.)DC polysaccharides,GDPs)进行硫酸酯化修饰,得到取代度为1.23的硫酸酯化多糖(sulfated GDPs,S-GDPs)。进一步分析GDPs和S-GDPs清除1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH)、超氧阴离子自由基(O2-·)、羟自由基(·OH)的能力,并考察两种多糖对H_2O_2诱导的红细胞氧化损伤以及·OH诱导的小鼠肝脏线粒体氧化损伤的保护作用。研究表明:GDPs和S-GDPs均具有抗氧化活性,其中S-GDPs清除DPPH(P<0.01)、O2-·(P<0.05)以及·OH(P<0.05)的能力显著高于GDPs;S-GDPs能显著降低红细胞溶血度(P<0.01)及其丙二醛含量(P<0.05),显著降低小鼠肝脏线粒体丙二醛含量(P<0.05)、极显著增加线粒体细胞膜的琥珀酸脱氢酶(SDH)和ATP酶(Na+-K+-ATPase、Ca~(2+)-Mg~(2+)-ATPase)活性(P<0.01),作用优于GDPs。由此可见,硫酸酯化修饰可以增强GDPs的抗氧化活性。
Sulfuric acid was used in the sulfating polysaccharides from Gynura davaricata(L.)DC.(GDPs),and substituted degree of sulfated polysaccharides(S-GDPs)of 1.23 was obtained.Then,the scavenging capacities of GDPs and S-GDPs against 1,1-diphenyl-2-picrylhydrazyl(DPPH),superoxide anion(O_2~-·)and hydroxyl free radicals(·OH)were tested.Protective effects of GDPs and S-GDPs on oxidative damage induced by H_2O_2 in erythrocytes or induced by ·OH in mice liver mitochondria were also determined.The results showed that S-GDPs had stronger antioxidative activities against DPPH(P<0.01),O_2~-·(P<0.05) and ·OH(P<0.05) than GDPs.At the same concentrations,S-GDPs could significantly inhibit oxidative hemolysis(P<0.01)and reduce malondialdehyde content(P<0.05)in erythrocyte.S-GDPs could significantly reduced MDA content of mitochondria(P<0.05),and improve the activities of succinate dehydrogenase(SDH)and ATPase(Na+-K+-ATPase and Ca~(2+)-Mg~(2+)-ATPase)activities(P<0.01)in mice liver mitochondria.All the above protective effects of S-GDPs is better than GDPs,which demostrated that the antioxidant activity of polysaccharides from Gynura davaricata can be improved through sulfation.
Sulfuric acid was used in the sulfating polysaccharides from Gynura davaricata(L.)DC.(GDPs),and substituted degree of sulfated polysaccharides(S-GDPs)of 1.23 was obtained.Then,the scavenging capacities of GDPs and S-GDPs against 1,1-diphenyl-2-picrylhydrazyl(DPPH),superoxide anion(O_2~-·)and hydroxyl free radicals(·OH)were tested.Protective effects of GDPs and S-GDPs on oxidative damage induced by H_2O_2 in erythrocytes or induced by ·OH in mice liver mitochondria were also determined.The results showed that S-GDPs had stronger antioxidative activities against DPPH(P<0.01),O_2~-·(P<0.05) and ·OH(P<0.05) than GDPs.At the same concentrations,S-GDPs could significantly inhibit oxidative hemolysis(P<0.01)and reduce malondialdehyde content(P<0.05)in erythrocyte.S-GDPs could significantly reduced MDA content of mitochondria(P<0.05),and improve the activities of succinate dehydrogenase(SDH)and ATPase(Na+-K+-ATPase and Ca~(2+)-Mg~(2+)-ATPase)activities(P<0.01)in mice liver mitochondria.All the above protective effects of S-GDPs is better than GDPs,which demostrated that the antioxidant activity of polysaccharides from Gynura davaricata can be improved through sulfation.
引文
[1]李小军,覃欢,刘欢.菊三七属植物的民族药用和食用价值[J].中南民族大学学报(自然科学版),2015(4):62-67.
[2]WU T T,ZHOU X T,DENG Y F,et al.In vitro studies of Gynura divaricata(L).D C extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension[J].J Ethnopharmacol.2011,136(2):305-308.
[3]WAN C,YU Y,ZHOU S,et al.Isolation and identification of phenolic compounds from Gynura divaricata leaves[J].Pharmacogn Mag,2011,7(26):101-108.
[4]陈磊,宋增艳,王津江,等.白背三七地上部分化学成分研究[J].中药材,2010,33(3):373-376.
[5]胡勇,李维林,林厚文,华燕,等.白背三七地上部分降血糖作用研究[J].西南林学院学报,2007,27(1):55-58.
[6]姜曼花,胡剑卓,邱文高,等.白背三七多糖和黄酮降血糖及耐缺氧作用[J].中国医院药学杂志,2009,29(13):1074-1076.
[7]昊霞,张钱钱,王忠震,等.白子菜叶片中酸性多糖的降血糖作用及其对相关指标的影响[J].植物资源与环境学报,2015,24(2):115-117.
[8]刘微微,刘旭,曹学丽.白背三七多糖的分离纯化[J].食品科学,2011,32(23):58-63.
[9]刘微微,刘旭,曹学丽,等.白背三七多糖的结构表征及α-葡萄糖苷酶的抑制活性[J].食品科学,2013,34(7):115-120.
[10]马正东,陈磊,宋洪涛,等.白背三七水提取物对2型糖尿病大鼠的降血糖作用及其机制[J].中草药,2010,41(4):623-626.
[11]LIU C M,CHEN H J,CHEN K,et al.Sulfated modification can enhance antiviral activities of Achyranthes bidentata polysaccharide against porcine reproductive and respiratory syndrome virus(PRRSV)in vitro[J].Int J Biol Macromol,2013,52(1):21-24.
[12]WEI D F,WEI Y X,CHENG W D,et al.Sulfated modification,characterization and antitumor activities of Radix hedysari polysaccharide[J].Int J Biol Macromol,2012,51(4):471-476.
[13]姚秋萍,李阳,邓健.硫酸酯化修饰的油菜花粉多糖的抗氧化活性[J].食品科学,2015,36(13):65-68.
[14]QIAN J Y,BAI Y Y,TANG J,et al.Antioxidation andα-glucosidase inhibitory activities of barley polysaccharides modified with sulfation[J].LWT-Food Sci Technol.2015,64(1):104-111.
[15]陈景耀,张卫明,王习达,等.白岌多糖硫酸醋化及生物学活性的比较研究[J].食品科学,2007,28(4):27-31.
[16]朱振元,原静,李盛峰,等.古尼虫草多糖硫酸酯化修饰及其抗氧化活性[J].现代食品科技,2011,27(1):1-5.
[17]王文侠,王龙艳,宋春丽,等.豆渣多糖硫酸酯化工艺条件优化及其抗氧化活性[J].食品与发酵工业,2013,39(1):103-107.
[18]朱淑云,沙莎,秦云云,等.水飞蓟寡肽对小鼠肝线粒体损伤的保护作用[J].中国粮油学报,2015,30(1):97-101.
[19]阚国仕,张瑞雪,陈红漫.胡萝卜多糖硫酸酯化修饰及其抗氧化活性研究[J].2012,37(11):170-173.
[20]Zhang Y L,Lu X Y,Fu Z B,et al.Sulphated modification of a polysaccharide obtained from fresh persimmon(Diospyros kaki L.)fruit and antioxidant activities of the sulphated derivatives[J].Food Chemistry,2011,127(3):1084-1090.
[21]秦双立,官志忠.线粒体损伤和氧化应激的关系[J].国外医学医学地理分册.2013,34(3):197-201.
[22]谢明勇,王之珺,谢建华.多糖的硫酸化修饰及其结构与生物活性关系研究进展[J].中国食品学报,2015,15(2):1-8.
[23]郭晓强,何钢,刘嵬,等.硫酸酯化银耳多糖的制备及抗氧化性活性研究[J].天然产物研究与开发,2013,25(6):747-751.
[24]刘军,白云凤,宫凯敏.猪苓多糖硫酸酯的制备及活性测定[J].中国实验方剂学杂志,2013,19(14):64-66.
[25]ZOU C,DU Y,LI Y,et al.Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro[J].Carbohydr Polym,2008,73(2):322-331.
[2]WU T T,ZHOU X T,DENG Y F,et al.In vitro studies of Gynura divaricata(L).D C extracts as inhibitors of key enzymes relevant for type 2 diabetes and hypertension[J].J Ethnopharmacol.2011,136(2):305-308.
[3]WAN C,YU Y,ZHOU S,et al.Isolation and identification of phenolic compounds from Gynura divaricata leaves[J].Pharmacogn Mag,2011,7(26):101-108.
[4]陈磊,宋增艳,王津江,等.白背三七地上部分化学成分研究[J].中药材,2010,33(3):373-376.
[5]胡勇,李维林,林厚文,华燕,等.白背三七地上部分降血糖作用研究[J].西南林学院学报,2007,27(1):55-58.
[6]姜曼花,胡剑卓,邱文高,等.白背三七多糖和黄酮降血糖及耐缺氧作用[J].中国医院药学杂志,2009,29(13):1074-1076.
[7]昊霞,张钱钱,王忠震,等.白子菜叶片中酸性多糖的降血糖作用及其对相关指标的影响[J].植物资源与环境学报,2015,24(2):115-117.
[8]刘微微,刘旭,曹学丽.白背三七多糖的分离纯化[J].食品科学,2011,32(23):58-63.
[9]刘微微,刘旭,曹学丽,等.白背三七多糖的结构表征及α-葡萄糖苷酶的抑制活性[J].食品科学,2013,34(7):115-120.
[10]马正东,陈磊,宋洪涛,等.白背三七水提取物对2型糖尿病大鼠的降血糖作用及其机制[J].中草药,2010,41(4):623-626.
[11]LIU C M,CHEN H J,CHEN K,et al.Sulfated modification can enhance antiviral activities of Achyranthes bidentata polysaccharide against porcine reproductive and respiratory syndrome virus(PRRSV)in vitro[J].Int J Biol Macromol,2013,52(1):21-24.
[12]WEI D F,WEI Y X,CHENG W D,et al.Sulfated modification,characterization and antitumor activities of Radix hedysari polysaccharide[J].Int J Biol Macromol,2012,51(4):471-476.
[13]姚秋萍,李阳,邓健.硫酸酯化修饰的油菜花粉多糖的抗氧化活性[J].食品科学,2015,36(13):65-68.
[14]QIAN J Y,BAI Y Y,TANG J,et al.Antioxidation andα-glucosidase inhibitory activities of barley polysaccharides modified with sulfation[J].LWT-Food Sci Technol.2015,64(1):104-111.
[15]陈景耀,张卫明,王习达,等.白岌多糖硫酸醋化及生物学活性的比较研究[J].食品科学,2007,28(4):27-31.
[16]朱振元,原静,李盛峰,等.古尼虫草多糖硫酸酯化修饰及其抗氧化活性[J].现代食品科技,2011,27(1):1-5.
[17]王文侠,王龙艳,宋春丽,等.豆渣多糖硫酸酯化工艺条件优化及其抗氧化活性[J].食品与发酵工业,2013,39(1):103-107.
[18]朱淑云,沙莎,秦云云,等.水飞蓟寡肽对小鼠肝线粒体损伤的保护作用[J].中国粮油学报,2015,30(1):97-101.
[19]阚国仕,张瑞雪,陈红漫.胡萝卜多糖硫酸酯化修饰及其抗氧化活性研究[J].2012,37(11):170-173.
[20]Zhang Y L,Lu X Y,Fu Z B,et al.Sulphated modification of a polysaccharide obtained from fresh persimmon(Diospyros kaki L.)fruit and antioxidant activities of the sulphated derivatives[J].Food Chemistry,2011,127(3):1084-1090.
[21]秦双立,官志忠.线粒体损伤和氧化应激的关系[J].国外医学医学地理分册.2013,34(3):197-201.
[22]谢明勇,王之珺,谢建华.多糖的硫酸化修饰及其结构与生物活性关系研究进展[J].中国食品学报,2015,15(2):1-8.
[23]郭晓强,何钢,刘嵬,等.硫酸酯化银耳多糖的制备及抗氧化性活性研究[J].天然产物研究与开发,2013,25(6):747-751.
[24]刘军,白云凤,宫凯敏.猪苓多糖硫酸酯的制备及活性测定[J].中国实验方剂学杂志,2013,19(14):64-66.
[25]ZOU C,DU Y,LI Y,et al.Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro[J].Carbohydr Polym,2008,73(2):322-331.