三参生血颗粒对~(60)Co照射所致C57BL/6小鼠造血及免疫系统不良反应治疗作用的研究
|
摘要
目的:研究三参生血颗粒对~(60)Co照射所致C57BL/6小鼠造血及免疫系统不良反应的治疗作用。方法:50只C57BL/6雄性小鼠随机分为正常组、模型组、高剂量组、中剂量组及低剂量组。采用~(60)Co照射对模型组、高剂量组、中剂量组及低剂量组小鼠进行造模,照射完成后,对各组小鼠进行给药处理,其中高剂量组、中剂量组及低剂量组小鼠分别灌胃给予6.15g/kg、3.075g/kg及1.5375g/kg的三参生血颗粒,空白组及模型组则给予等量生理盐水,连续给药15d。末次给药后24h采血,检测其中血象指标含量[红细胞计数(RBC)、白细胞计数(WBC)、血小板计数(PLT)及血红蛋白(Hgb)],氧化应激指标含量[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)及过氧化氢酶(CAT)],免疫功能指标含量[CD_3~+、CD_4~+、IgG、IgA及IgM]。取血完成后,取小鼠胸腺和脾脏,计算胸腺和脾脏指数。同时取小鼠胸骨及股骨,检测骨髓嗜多染红细胞微核率及有核细胞数。结果:与模型组比,高剂量组、中剂量组及低剂量组小鼠RBC、WBC、PLT及Hgb含量均显著降低(P<0.05),CD_3~+、CD_4~+、IgG、IgA及IgM含量均显著增高(P<0.05),且胸腺和脾脏指数均显著增高(P<0.05)。与模型组比,高剂量组、中剂量组及低剂量组小鼠骨髓嗜多染红细胞微核率均显著降低,而有核细胞数均显著增高(P<0.05)。与模型组比,高剂量组、中剂量组及低剂量组小鼠MDA均显著降低,而SOD、GSH-PX及CAT均显著增高(P<0.05)。结论:三参生血颗粒对~(60)Co照射所致C57BL/6小鼠造血及免疫系统不良反应具有较好的治疗作用。
Objective:To observe the therapeutical effects of Sanshen Shengxue granules on ~(60)Co irradiation induced hematopoietic and immune system adverse reactions in C57 BL/6 mice.Methods:50 C57 BL/6 male mice were divided into control group,model group,high dose group,middle dose group and low dose group.~(60)Co irradiation method was used in model group,high dose group,middle dose group and low dose group to build the model.After the irradiation,the high,middle and low dose groups were treated by Sanshen Shengxue granules with the dose of 6.15 g/kg,3.075 g/kg and 1.5375 g/kg,while the other groups were given equivalence saline,the treatment continues 15 days.24 hafter the last treatment,the serum was obtained,and the hemogram index[red blood cell count(RBC),white blood cell count(WBC),platelet count(PLT)and hemoglobin(Hgb)],oxidative stress indicators[malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(gsh-px)and catalase(CAT)],immune function index content[CD_3~+,CD_4~+,IgG,IgA and IgM]were detected in the groups.After the blood collection,the thymus and spleen index were observed,while the levels of micronucleus rate and number of nucleated cells were detected.Results:Compared with the model group,the content of RBC,WBC,PLT and Hgb were significantly higher in the high,middle and low dose groups(P<0.05),CD_3~+,CD_4~+,IgG,IgA and IgM levels were also significantly higher(P<0.05),while the thymus and spleen index were significantly higher(P<0.05).Compared with the model group,the levels of micronucleus rate were significantly higher and numbers of nucleated cells were significantly lower in the high,middle and low dose groups(P<0.05).Compared with the model group,the levels of MDA were significantly lower,while SOD,gsh-px and CAT levels were significantly higher in the high,middle and low dose groups(P<0.05).Conclusions:Sanshen Shengxue granules on ~(60)Co irradiation induced hematopoietic and immune system adverse reactions has a good effect.
Objective:To observe the therapeutical effects of Sanshen Shengxue granules on ~(60)Co irradiation induced hematopoietic and immune system adverse reactions in C57 BL/6 mice.Methods:50 C57 BL/6 male mice were divided into control group,model group,high dose group,middle dose group and low dose group.~(60)Co irradiation method was used in model group,high dose group,middle dose group and low dose group to build the model.After the irradiation,the high,middle and low dose groups were treated by Sanshen Shengxue granules with the dose of 6.15 g/kg,3.075 g/kg and 1.5375 g/kg,while the other groups were given equivalence saline,the treatment continues 15 days.24 hafter the last treatment,the serum was obtained,and the hemogram index[red blood cell count(RBC),white blood cell count(WBC),platelet count(PLT)and hemoglobin(Hgb)],oxidative stress indicators[malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(gsh-px)and catalase(CAT)],immune function index content[CD_3~+,CD_4~+,IgG,IgA and IgM]were detected in the groups.After the blood collection,the thymus and spleen index were observed,while the levels of micronucleus rate and number of nucleated cells were detected.Results:Compared with the model group,the content of RBC,WBC,PLT and Hgb were significantly higher in the high,middle and low dose groups(P<0.05),CD_3~+,CD_4~+,IgG,IgA and IgM levels were also significantly higher(P<0.05),while the thymus and spleen index were significantly higher(P<0.05).Compared with the model group,the levels of micronucleus rate were significantly higher and numbers of nucleated cells were significantly lower in the high,middle and low dose groups(P<0.05).Compared with the model group,the levels of MDA were significantly lower,while SOD,gsh-px and CAT levels were significantly higher in the high,middle and low dose groups(P<0.05).Conclusions:Sanshen Shengxue granules on ~(60)Co irradiation induced hematopoietic and immune system adverse reactions has a good effect.
引文
[1]张含凤,张海霖,杨志芬,等.放疗不良反应预测因素的研究进展[J].中华现代护理杂志,2016,22(7):1033-1036.
[2]张钱钱,杨丽娜,宋洪涛.癌症放化疗不良反应的中医药治疗[J].中国癌症防治杂志,2014,6(2):208-210.
[3]安鹏,李星瑶,蔡子墨,等.放疗与中药"君-佐"相伍的理论运用于肝癌细胞放疗增敏效果分析[J].陕西中医,2017,38(11):1610-1612.
[4]于纯淼,王贺,赵力松,等.不同辐照剂量率X射线对小鼠外周血细胞及免疫器官的影响[J].实验动物与比较医学,2017,37(2):140-143.
[5]许世娟,杜娟,滕志.PEG-rhG-CSF预处理对BUCY方案致小鼠骨髓抑制模型骨髓造血恢复的影响[J].现代肿瘤医学,2016,24(9):1337-1340.
[6]田源,张红志.肿瘤放射治疗技术进展[J].中华结直肠疾病电子杂志,2016,5(4):287-291.
[7]张燕,蒋扬,贾绍昌.放射治疗对恶性肿瘤患者免疫系统调节作用的研究进展[J].中华临床医师杂志:电子版,2014,8(21):120-123.
[8]颜桂明,陈国付,张飞燕.肿瘤精确放疗技术进展[J].医学理论与实践,2015,28(2):164-166.
[9]秦雨琪,张丽娟,任伟东,等.骨髓干细胞输注修复放疗免疫抑制的动物实验[J].昆明医科大学学报,2017,38(6):9-14.
[10]吴宏,闫国诚,王双全,等.姜黄素对胃癌细胞放疗增敏作用的研究[J].陕西医学杂志,2014,43(2):137-139.
[11]康静波.肿瘤精确放射治疗的技术进展与临床应用[J].转化医学杂志,2016,5(2):65-69.
[12]徐薇,刘秀华,陈钢.从“少阳春生之气”理论探讨调肝生血法对放疗后小鼠造血功能的调控作用[J].时珍国医国药,2016,27(1):45-46.
[13]赵崇伟,王阁.肿瘤放疗免疫反应机制研究新进展[J].重庆医学,2014,43(30):4107-4110.
[14]陈士林,刘安,李琦.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.
[15]刘骏方,胡金香.介入放射学中的中药应用及药理学研究[J].中国中西医结合影像学杂志,2016,14(6):734-737.
[2]张钱钱,杨丽娜,宋洪涛.癌症放化疗不良反应的中医药治疗[J].中国癌症防治杂志,2014,6(2):208-210.
[3]安鹏,李星瑶,蔡子墨,等.放疗与中药"君-佐"相伍的理论运用于肝癌细胞放疗增敏效果分析[J].陕西中医,2017,38(11):1610-1612.
[4]于纯淼,王贺,赵力松,等.不同辐照剂量率X射线对小鼠外周血细胞及免疫器官的影响[J].实验动物与比较医学,2017,37(2):140-143.
[5]许世娟,杜娟,滕志.PEG-rhG-CSF预处理对BUCY方案致小鼠骨髓抑制模型骨髓造血恢复的影响[J].现代肿瘤医学,2016,24(9):1337-1340.
[6]田源,张红志.肿瘤放射治疗技术进展[J].中华结直肠疾病电子杂志,2016,5(4):287-291.
[7]张燕,蒋扬,贾绍昌.放射治疗对恶性肿瘤患者免疫系统调节作用的研究进展[J].中华临床医师杂志:电子版,2014,8(21):120-123.
[8]颜桂明,陈国付,张飞燕.肿瘤精确放疗技术进展[J].医学理论与实践,2015,28(2):164-166.
[9]秦雨琪,张丽娟,任伟东,等.骨髓干细胞输注修复放疗免疫抑制的动物实验[J].昆明医科大学学报,2017,38(6):9-14.
[10]吴宏,闫国诚,王双全,等.姜黄素对胃癌细胞放疗增敏作用的研究[J].陕西医学杂志,2014,43(2):137-139.
[11]康静波.肿瘤精确放射治疗的技术进展与临床应用[J].转化医学杂志,2016,5(2):65-69.
[12]徐薇,刘秀华,陈钢.从“少阳春生之气”理论探讨调肝生血法对放疗后小鼠造血功能的调控作用[J].时珍国医国药,2016,27(1):45-46.
[13]赵崇伟,王阁.肿瘤放疗免疫反应机制研究新进展[J].重庆医学,2014,43(30):4107-4110.
[14]陈士林,刘安,李琦.中药饮片标准汤剂研究策略[J].中国中药杂志,2016,41(8):1367-1375.
[15]刘骏方,胡金香.介入放射学中的中药应用及药理学研究[J].中国中西医结合影像学杂志,2016,14(6):734-737.