利用抗旱选择导入系定位向日葵产量性状QTL
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摘要
干旱是造成向日葵减产的最主要因素之一。利用综合性状优良的自交系K55作为轮回亲本与抗旱自交系K58杂交构建回交导入系,在干旱条件下进行单株产量筛选,得到45个BC3F2抗旱定向选择导入系。通过全基因组SSR及SNP标记扫描,以方差分析和基于遗传搭车原理的卡方检验对呼和浩特市及武川县两点、两种水分条件下的5个产量性状进行QTL检测。方差分析检测到的QTL根据不同环境下的表达情况分为三类,第一类在两种水分条件下稳定表达,包括武川的4个百粒重QTL及呼和浩特的2个单株产量QTL、3个单株实粒数QTL,这些QTL可能对向日葵抗旱性有直接贡献;第二类受干旱胁迫表达,包括呼和浩特的30个和武川的27个;第三类仅在正常供水条件下被检测到,包括呼和浩特的38个和武川的64个。卡方检验检测到极显著位点274个。用两种方法共检测到一致性位点14个,可能是与向日葵抗旱性相关的关键位点。本研究结果可为向日葵高效抗旱分子育种奠定基础并提供相关材料。
Drought is one of the most important factors to decrease the yield of sunflower. The BC3 F2 selected backcrossing introgression lines of oil sunflower population including 45 lines were developed by drought-tolerance screening for yield using Helianthus annuus K55 with excellent comprehensive characters and drought sensitivity as recurrent parent and K58 with drought-tolerance as donor parent. The genotypes of selected backcrossing introgression lines were obtained with the whole genome SSR and SNP markers. QTLs affecting five yield traits were detected under both drought stress and well watered conditions in Hohhot and Wuchuan respectively by one-way ANOVA and Chi-square test based on the Genetic Hitchhiking Effect. The QTLs detected by one-way ANOVA were grouped into three types based on their behaviors: type I was the QTLs detected in both watered conditions, including four QTLs affecting hundred-seed weight(HSW) in Wuchuan and two QTLs affecting seed yield(SY) and three QTLs affecting filled seeds per plant(FSP) in Hohhot, which were considered to be able to directly contribute to drought tolerance; type II was the QTLs detected only under drought stress, including 30 QTLs in Hohhot and 27 QTLs in Wuchuan; type III was the QTLs detected only in well watered condition, including 38 QTLs in Hohhot and 64 QTLs in Wuchuan. There 274 loci were detected by chi-square test, among them 14 loci could be detected by ANOVA and chi-square test simultaneously, which might be the key loci for drought tolerance of sunflower. The results lay a foundation of efficient drought tolerance molecular breeding and provide useful drought tolerance materials for sunflower.
Drought is one of the most important factors to decrease the yield of sunflower. The BC3 F2 selected backcrossing introgression lines of oil sunflower population including 45 lines were developed by drought-tolerance screening for yield using Helianthus annuus K55 with excellent comprehensive characters and drought sensitivity as recurrent parent and K58 with drought-tolerance as donor parent. The genotypes of selected backcrossing introgression lines were obtained with the whole genome SSR and SNP markers. QTLs affecting five yield traits were detected under both drought stress and well watered conditions in Hohhot and Wuchuan respectively by one-way ANOVA and Chi-square test based on the Genetic Hitchhiking Effect. The QTLs detected by one-way ANOVA were grouped into three types based on their behaviors: type I was the QTLs detected in both watered conditions, including four QTLs affecting hundred-seed weight(HSW) in Wuchuan and two QTLs affecting seed yield(SY) and three QTLs affecting filled seeds per plant(FSP) in Hohhot, which were considered to be able to directly contribute to drought tolerance; type II was the QTLs detected only under drought stress, including 30 QTLs in Hohhot and 27 QTLs in Wuchuan; type III was the QTLs detected only in well watered condition, including 38 QTLs in Hohhot and 64 QTLs in Wuchuan. There 274 loci were detected by chi-square test, among them 14 loci could be detected by ANOVA and chi-square test simultaneously, which might be the key loci for drought tolerance of sunflower. The results lay a foundation of efficient drought tolerance molecular breeding and provide useful drought tolerance materials for sunflower.
引文
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[2]王阳,刘成,王天宇,石云素,宋燕春,黎裕.干旱胁迫和正常灌溉条件下玉米产量性状的QTL分析.植物遗传资源学报,2007,8:179–183Wang Y,Liu C,Wang T Y,Shi Y S,Song Y C,Li Y.QTL analysis of yield components in maize under different water regimes.J Plant Genet Resour,2007,8:179–183(in Chinese with English abstract)
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[4]Adiredjo A L,Navaud O,Munos S,Langlade N B,Lamaze T,Grieu P.Genetic control of water use efficiency and leaf carbon isotope discrimination in sunflower(Helianthus annuu L.)subjected to two drought scenarios.PLo S One,2014,9:e101218
[5]Abdi1 N,Darvishzadeh R,Jafaril M,Pirzad A,Haddadi P.Genetic analysis and QTL mapping of agro-morphological traits in sunflower(Helianthus annuus L.)under two contrasting water treatment conditions.Plant Omics J,2012,5:149–158
[6]Haddadi P,Yazdi-samadi B,Naghavi M R,Kalantari A,Maury P,Sarrafi A.QTL analysis of agronomic traits in recombinant inbred lines of sunflower under partial irrigation.Plant Biotechnol Rep,2011,5:135–146
[7]吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华.向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析.作物学报,2017,43:19–30Lyu P,Yu H F,Yu Z X,Zhang Y H,Zhang Y F,Wang T T,Hu J H.Construction of high-density genetic map and qtl mapping for seed germination traits in sunflower under two water conditions.Acta Agron Sin,2017,43:19–30(in Chinese with English abstract)
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[9]刘海燕.水稻高代回交导入系抗旱相关性状QTL定位研究.中国农业科学院硕士学位论文,北京,2009Liu H Y.QTL Mapping of Traits Related to Drought Tolerance of Advanced Backcrossing Introgression Lines in Rice.MS Thesis of Chinese Academy of Agricultural Sciences,Beijing,China,2009(in Chinese with English abstract)
[10]黎志康.我国水稻分子育种计划的策略.分子植物育种,2005,3:603–608Li Z K.Strategies for molecular rice breeding in China.Mol Plant Breed,2005,3:603–608
[11]卓壮.黄华占回交导入系抗旱筛选及QTL分析.四川农业大学硕士学位论文,四川雅安,2013Zhuo Z.Screening and QTL Mapping of Advanced Backcrossing Introgression Lines for Drought Tolerance in Huang Huazhan.MS Thesis of Sichuan Agricultural University,Sichuan Ya’an,China,2013(in Chinese with English abstract)
[12]赵秀琴,朱苓华,徐建龙,黎志康.灌溉与自然降雨条件下水稻高代回交导入系产量QTL的定位.作物学报,2007,33:1536–1542Zhao X Q,Zhu L H,Xu J L,Li Z K.QTL mapping of yield under irrigation and rainfed field conditions for advanced backcrossing introgression lines in rice.Acta Agron Sin.2007,33:1536–1542(in Chinese with English abstract)
[13]王利锋,郝宪彬,何云霞,华泽田,高用明,黎志康.利用选择回交导入群体进行粳稻优良恢复系抗旱性改良与QTL定位.安徽农业大学学报,2007,34:311–319Wang L F,Hao X B,He Y X,Hua Z T,Gao Y M,Li Z K.Improvement of drought tolerance for elite restorer of japonica rice(Oryza sativa L.)and identification of drought tolerant QTLs using selected introgression populations by backcross.J Anhui Agric Univ,2007,34:311–319(in Chinese with English abstract)
[14]王英.利用回交导入系筛选水稻高产、抗旱和耐盐株系及选择导入系相关性状的QTL定位.中国农业科学院博士学位论文,北京,2013Wang Y.Screening of High Yield,Drought and Salt Tolerant Plants from Backcross Introgression Lines and QTL Detection for Related Traits in Rice.Ph D Dissertation of Chinese Academy of Agricultural Sciences,Beijing,China,2013(in Chinese with English abstract)
[15]任洁,赵秀琴,丁在松,项超,张晶,王超,张俊巍,Joseph C A,张强,庞昀龙,高用明,石英尧.利用选择导入系进行水稻耐低磷鉴定与QTL定位分析.中国水稻科学,2015,29:1–13Ren J,Zhao X Q,Ding Z S,Xiang C,Zhang J,Wang C,Zhang J W,Joseph C A,Zhang Q,Pong Y L,Gao Y M,Shi Y Y.Dissection and QTL mapping of low-phosphorus tolerance using selected introgression lines in rice.Chin J Rice Sci,2015,29:1–13(in Chinese with English abstract)
[16]李芳,程立锐,许美容,周政,张帆,孙勇,周永力,朱苓华,徐建龙,黎志康.利用品质性状的回交选择导入系挖掘水稻抗纹枯病QTL.作物学报,2009,35:1729-1737Li F,Cheng L R,Xu M R,Zhou Z,Zhang F,Sun Y,Zhou Y L,Zhu L H,Xu J L,Li Z K.QTL mining for sheath blight resistance using the backcross selected introgression lines for grain quality in rice.Acta Agron Sin,2009,35:1729–1737(in Chinese with English abstract)
[17]李灿东,蒋洪蔚,刘春燕,邱鹏程,张闻博,李文福,高运来,陈庆山,胡国华.大豆定向选择群体耐旱性位点基因型分析及QTL定位.中国油料作物学报,2009,31:285–292Li C D,Jiang H W,Liu C Y,Qiu P C,Zhang W B,Li T F,Gao Y L,Chen Q S.Genotype and QTL analysis of drought tolerance loci for directional population in soybean.Chin J Oil Crop Sci,2009,31:285–292(in Chinese with English abstract)
[18]张金巍,韩粉霞,陈明阳,孙君明,韩广振,闫淑荣,杨华.利用选择回交导入群体定位大豆蛋白质含量QTL.中国油料作物学报,2015,37:433–442Zhang J W,Han F X,Chen M Y,Sun J M,Han G Z,Yan S R,Yang H.Detection of protein content QTL with random and extremely selected BC2F2 populations in soybean.Chin J Oil Crop Sci,2015,37:433–442(in Chinese with English abstract)
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