大豆荚粒数相关QTL的Meta和Overview分析及其候选基因预测
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摘要
荚粒性状与大豆(Glycine max)产量密切相关。本研究利用完备区间作图法(inclusive composite interval mapping, ICIM)对两年的重组自交系(recombinant inbred line, RIL)群体数据进行分析,获得7个与荚粒性状相关的数量性状基因座(quantitative trait loci, QTL),并搜集了国内外已报道的154个荚粒性状相关QTLs。利用元分析(Meta-analysis)方法得到23个Meta-QTL区间,利用基于统计学原理的Overview方法对这些QTL区间进行优化。经Overview分析,获得13个有效的QTL区间,5个QTL区间被缩小到0.5 Mb。对Meta-QTL区间进行基因注释,获得687个基因,筛选11个基因作为候选基因。11个候选基因参与植物生长发育、果实成熟等过程。本研究建立的实验方法及研究结果,对于大豆荚粒性状QTL定位及分子辅助育种提供了基础资料。
Seed and pod traits are closely related to seed yield of soybean(Glycine max). The present study used the interval complete interval mapping method(ICIM) to analyze the recombinant inbred line(RIL) population phenotypic data across two-year, and obtained 7 quantitative trait loci(QTL) related to the seed and pod traits.154 original QTLs related to seed and pod trait of soybean from database and references were analyzed. 23 MetaQTL intervals were obtained by Meta-analysis. Moreover, the Overview method was used to optimize these QTLs based on statistical analysis. After the Overview analysis, 13 valid QTL regions were obtained, and 5 QTL regions were narrowed down to 0.5 Mb. Furthermore, genetic annotation for the Meta-QTL interval and 687 genes were obtained, and 11 genes were selected as candidate genes. 11 candidate genes participated in the process of plant growth and fruit maturation. The experimental method of this study and results are important for QTL mapping associated with seed and pod traits and assisted molecular breeding of soybeans.
Seed and pod traits are closely related to seed yield of soybean(Glycine max). The present study used the interval complete interval mapping method(ICIM) to analyze the recombinant inbred line(RIL) population phenotypic data across two-year, and obtained 7 quantitative trait loci(QTL) related to the seed and pod traits.154 original QTLs related to seed and pod trait of soybean from database and references were analyzed. 23 MetaQTL intervals were obtained by Meta-analysis. Moreover, the Overview method was used to optimize these QTLs based on statistical analysis. After the Overview analysis, 13 valid QTL regions were obtained, and 5 QTL regions were narrowed down to 0.5 Mb. Furthermore, genetic annotation for the Meta-QTL interval and 687 genes were obtained, and 11 genes were selected as candidate genes. 11 candidate genes participated in the process of plant growth and fruit maturation. The experimental method of this study and results are important for QTL mapping associated with seed and pod traits and assisted molecular breeding of soybeans.
引文
陈玉福.2003.高寒地区大豆主要经济性状遗传相关和选择指数的研究[J].西北农业学报,12(2):51-54.(Chen YF.2003.The study on genetic correlation and selection index of soybeans main economic character in high and cold area[J].Acta Agriculturae Boreali-Occidentalis Sinica,12(2):51-54.)
戴颐和.1982.夏大豆荚粒性状遗传变异、遗传相关和选择指数研究的初报[J].安徽农业科学,46-50.(Dai Y H.1982.A preliminary report on genetic variation,genetic correlation and selection index of summer soybean Alfalfa grain traits[J].Journal of Anhui Agricultural Sciences,46-50.)
高利芳,郭勇,郝再彬,等.2013.大豆株高QTL的"整合"及Overview分析[J].遗传,35(2):215-224.(Gao L F,Guo Y,Hao Z B,et al.2013.Integration and"Overview"analysis of QTLs related to plant height in soybean[J].Hereditas,35(2):215-224.)
晁毛妮,郝德荣,印志同,等.2014.大豆生物量与产量组分间的相关及关联分析[J].作物学报,40(1):7-16.(Huang M N,Hao D R,Yin Z T et al.2014.Correlation and association analysis between biomass and yield components in soybean[J].Acta Agronomica Sinica,40(1):7-16.)
李灿东,蒋洪蔚,张闻博,等.2008.大豆荚粒相关性状的QTL分析[J].分子植物育种,6(6):1091-1100.(Li C D,Jiang H W,Zhang W B,et al.2008.QTL analysis of seed and pod traits in soybean molecular plant breeding,6(6):1091-1100.)
刘硕,罗玲,刘章雄,等.2011.大豆蛋白质含量QTL的"整合"及Overview分析[J].大豆科学,30(1):1-7.(Liu S,Luo L,Liu Z X,et al.2011.Integration of QTLs related to soybean protein content and"qualification"of them by overview method[J].Soybean Science,30(1):1-7.)
刘硕,郭勇,罗玲,等.2014.大豆倒伏性相关QTL的整合及Overview分析[J].植物遗传资源学报,15(1):137-143.(Liu S,Guo Y,Luo L,et al.2014.Integration and overview analysis of QTLs related to lodging in soybean(Glycine max)[J].Journal of Plant Genetic Resources,15(1):137-143.)
邱红梅,厉志,于妍,等.2015.基于元分析的大豆含硫氨基酸相关基因挖掘与信息学分析[J].中国油料作物学报,37(2):141-147.(Qiu H M,Li Z,Yu Y et al.2015.Mining and analysis of genes related to sulfur-containing amino acids in soybean based on Meta-QTL[J].Chinese Journal of Oil Crop Sciences,37(2):141-147.)
位艳丽.2011.大豆农艺和品质性状遗传模型分析与QTL定位[D].硕士学位论文,河南农业大学,导师:梁惠珍,pp.35-41.(Wei Y L.2011.Genetic model analysis and QTL mapping of agronomic and quality traits in soybean[D].Thesis for M.S.,Henan Agriculture University,Supervisor:Liang H Z,pp.35-41.)
王贤智,张晓娟,周蓉,等.2007.大豆重组自交系群体荚粒性状的QTL分析[J].作物学报,33(3):441-448.(Wang X Z,Zhang X J,Zhou R,et al.2007.QTL analysis of seed and pod traits in soybean RIL population[J].Acta Agronomica Sinica,33(3):441-448.)
张富厚,郑跃进,侯典云,等.2006.大豆荚粒性状对单株产量的效应分析[J].安徽农业科学,34(15):3632-3633.(Zhang F H,Zheng Y J,Hou D Y,et al.2006.Effect of soybean pod traits on yield per plant[J].Journal of Anhui Agricultural Sciences,34(15):3632-3633.)
张维耀,王金星,宗春美,等.2012.大豆荚粒性状的QTL分析[J].大豆科学,31(2):193-197.(Zhang W Y,Wang JX,Zong C M,et al.2012.QTL analysis of seed and pod traits in soybean[J].Soybean Science,31(2):193-197.)
Arcade A,Labourdette A,Falque M,et al.2004.BioMercator:Integrating genetic maps and QTL towards discovery of candidate genes[J].Bioinformatics.20(14):2324-2326.
Chardon F,Virlon B,Moreau L,et al.2004.Genetic architecture of flowering time in maize as inferred from quantitative trait loci meta-analysis and synteny conservation with the rice genome[J].Genetics,168(4):2169-2185.
Chen Q S,Zhang Z C,Liu C Y et al.2007.QTL analysis of major agronomic traits in soybean[J].Agricultural Sciences in China,6(4):399-405.
Darvasi A,Soller M.1997.A simple method to calculate resolving power and confidence interval of QTL map location[J].Behavior Genetics,27(2):125-132.
Goffinet B,Gerber S.2000.Quantitative trait loci:A metaanalysis[J].Genetics,155(1):463-473.
Geisler D A,P?pke C,Obata T,et al.2012.Downregulation of theδ-subunit reduces mitochondrial ATP synthase levels,alters respiration,and restricts growth and gametophyte development in Arabidopsis[J].Plant Cell,24(7):2792-2811.
He J N,Duan Y,Hua D P,et al.2012.DEXH box RNA helicase-mediated mitochondrial reactive oxygen species production in Arabidopsis mediates crosstalk between abscisic acid and auxin signaling[J].Plant Cell,24(5):1815-1833.
Iwata Y J,Fedoroff N V,Koizumi N.2008.Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response[J].Plant Cell,20(11):3107-3121.
Jeong N,Suh S J,Kim M H,et al.2012.Ln is a key regulator of leaflet shape and number of seeds per pod in soybean[J].Plant Cell,24(12):4807-4818.
Ji X Y,Liu G F,Liu Y J,et al.2015.The regulatory network of ThbZIP1 in response to abscisic acid treatment[J].Frontiers in Plant Science,6(25):1-12
Kandoth P K,Ithal N,Recknor J,et al.2011.The soybean Rhg1 locus for resistance to the soybean cyst nematode heterodera glycines regulates the expression of a large number of stress-and defense-related genes in degenerating feeding cells[J].Plant Physiology,155(4):1960-1975.
Kuroda Y,Kaga A,Tomooka N,et al.2013.QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields[J].Ecology and Evolution,3(7):2150-2168.
Glass G V,Smith M L.1979.Meta-analysis of research on class size and achievement[J].Educational Evaluation&Policy Analysis,1(1):2-16.
Li,D,Sun M,Han Y,et al.(2010).Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold(Sclerotinia sclerotiorum)[J].Euphytica,172(1):49-57.
Li F L,Asami T D,Wu X Z,et al.2007.A putative hydroxysteroid dehydrogenase involved in regulating plant growth and development[J].Plant Physiology,145(1):87-97.
Liu N X,Li M,Hu X B,et al.2017.Construction of high-density genetic map and QTL mapping of yield-related and two quality traits in soybean RILs population by RAD-sequencing[J].BMC Genomics,18(1):466-478.
Liu W X,Kim M Y,Van K,et al.2011.QTL identification of yield-related traits and their association with flowering and maturity in soybean[J].Journal of Crop Science and Biotechnology,14(1):65-70.
Liu S,GuoY,Luo L,et al.2014.Integration and"Overview"analysis of QTLs related to lodging in soybean(Glycine max)[J].Journal of Plant Genetic Resources,15(1):137-143.
Liu Z,Yao D S,Zhang J,et al.2015.Identification of genes associated with the increased number of four-seed pods in soybean(Glycine max L.)using transcriptome analysis[J].Genetics and Molecular Research,14(4):18895-18912.
Merelo P,AgustíJ,Arbona V,et al.2017.Corrigendum:Cell wall remodeling in abscission zone cells during ethylene-promoted fruit abscission in citrus[J].Frontiers in Plant Science,8(126):301-320.
Murphy T M,Belmonte M,Shu S,et al.2009.Requirement for abasic endonuclease gene homologues in Arabidopsis seed development[J].PLoS One,4(1):e4297.
Mukhtar F A,Coyne D P.1981.Inheritance and association of flower,ovule,seed,pod,and maturity characters in dry edible beans(Phaseolus vulgaris L)[J].Journal American Society for Horticultural Science,106(6):713-719.
Palomeque L,Li J L,Li W B,et al.2009.QTL in mega-environments:II.agronomic trait QTL co-localized with seed yield QTL detected in a population derived from a cross of high-yielding adapted x high-yielding exotic soybean lines[J].Theoretical&Applied Genetics,119(3):429-436.
Paque S,Mouille G,Grandont L,et al.2014.Auxin binding protein1 links cell wall remodeling,auxin signaling,and cell expansion in Arabidopsis[J].Plant Cell,26(1):280-295.
Qi Z M,Huang L,Zhu R S,et al.2014.A high-density genetic map for soybean based on specific length amplified fragment sequencing[J].PLoS One,9(8):e104871.
Qi Z M,Wu Q,Han X et al.2011.Soybean oil content QTLmapping and integrating with meta-analysis method for mining genes[J].Euphytica,179(3):499-514.
Ragin B,Richard B,Willsheana C,et al.2012.Genetic analysis of yield components in the PI 438489B by'Hamilton'recombinant inbred line(RIL)population of soybean[Glycine max(L.)Merr.][J].Journal of Agricultural Science,4(9):98-105.
Shi L Y,Li X H,Hao Z F,et al.2007.Comparative QTL mapping of resistance to gray leaf spot in maize based on bioinformatics[J].Agricultural Sciences in China,6(12):1411-1419.
Sun D S,Li W B,Zhang Z C,et al.2006.Quantitative trait loci analysis for the developmental behavior of soybean(Glycine max L.Merr.)[J].Theoretical&Applied Genetics,112(4):665-673.
Swamy B M,Sarla N.2011.Meta-analysis of yield QTLs derived from inter-specific crosses of rice reveals consensus regions and candidate genes[J].Plant Molecular Biology Reporter,29(3):663-680.
Vieira A J D,Oliveira D A,Soares T C B,et al.2006.Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7and F8generations[J].Brazilian Journal of Plant Physiology,2006,18(2):281-290.
Wang X B,Zhang H W,Gao Y L,et al.2015.Evolution and association analysis of GmCYP78A10 gene with seed size/weight and pod number in soybean[J].Molecular Biology Reports,42(2):489-496.
Wang Y,Yao J,Zhang Z F,et al.2006.The comparative analysis based on maize integrated QTL map and meta-analysis of plant height QTLs[J].Chinese Science Bulletin,51(18):2219-2230.
Xu P,Wu X H,Wang B G,et al.2013.QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits.BMC Genetics,14(1):4-13.
Yang Z,Xin D W,Liu CY,et al.2013.Identification of QTLs for seed and pod traits in soybean and analysis for additive effects and epistatic effects of QTLs among multiple environments[J].Molecular Genetics and Genomics,288(12):651-667.
Zhang D,Cheng H,Wang H,et al.2010.Identification of genomic regions determining flower and pod numbers development in soybean(Glycine max L.)[J].Journal of Genetics and Genomics,37(8):545-556.
Zhang Y X,Liu S M,Dai S Y,et al.2012.Integration of shotgun proteomics and bioinformatics analysis to explore plant hormone responses[J].BMC Bioinformatics,13(15):8-18.
戴颐和.1982.夏大豆荚粒性状遗传变异、遗传相关和选择指数研究的初报[J].安徽农业科学,46-50.(Dai Y H.1982.A preliminary report on genetic variation,genetic correlation and selection index of summer soybean Alfalfa grain traits[J].Journal of Anhui Agricultural Sciences,46-50.)
高利芳,郭勇,郝再彬,等.2013.大豆株高QTL的"整合"及Overview分析[J].遗传,35(2):215-224.(Gao L F,Guo Y,Hao Z B,et al.2013.Integration and"Overview"analysis of QTLs related to plant height in soybean[J].Hereditas,35(2):215-224.)
晁毛妮,郝德荣,印志同,等.2014.大豆生物量与产量组分间的相关及关联分析[J].作物学报,40(1):7-16.(Huang M N,Hao D R,Yin Z T et al.2014.Correlation and association analysis between biomass and yield components in soybean[J].Acta Agronomica Sinica,40(1):7-16.)
李灿东,蒋洪蔚,张闻博,等.2008.大豆荚粒相关性状的QTL分析[J].分子植物育种,6(6):1091-1100.(Li C D,Jiang H W,Zhang W B,et al.2008.QTL analysis of seed and pod traits in soybean molecular plant breeding,6(6):1091-1100.)
刘硕,罗玲,刘章雄,等.2011.大豆蛋白质含量QTL的"整合"及Overview分析[J].大豆科学,30(1):1-7.(Liu S,Luo L,Liu Z X,et al.2011.Integration of QTLs related to soybean protein content and"qualification"of them by overview method[J].Soybean Science,30(1):1-7.)
刘硕,郭勇,罗玲,等.2014.大豆倒伏性相关QTL的整合及Overview分析[J].植物遗传资源学报,15(1):137-143.(Liu S,Guo Y,Luo L,et al.2014.Integration and overview analysis of QTLs related to lodging in soybean(Glycine max)[J].Journal of Plant Genetic Resources,15(1):137-143.)
邱红梅,厉志,于妍,等.2015.基于元分析的大豆含硫氨基酸相关基因挖掘与信息学分析[J].中国油料作物学报,37(2):141-147.(Qiu H M,Li Z,Yu Y et al.2015.Mining and analysis of genes related to sulfur-containing amino acids in soybean based on Meta-QTL[J].Chinese Journal of Oil Crop Sciences,37(2):141-147.)
位艳丽.2011.大豆农艺和品质性状遗传模型分析与QTL定位[D].硕士学位论文,河南农业大学,导师:梁惠珍,pp.35-41.(Wei Y L.2011.Genetic model analysis and QTL mapping of agronomic and quality traits in soybean[D].Thesis for M.S.,Henan Agriculture University,Supervisor:Liang H Z,pp.35-41.)
王贤智,张晓娟,周蓉,等.2007.大豆重组自交系群体荚粒性状的QTL分析[J].作物学报,33(3):441-448.(Wang X Z,Zhang X J,Zhou R,et al.2007.QTL analysis of seed and pod traits in soybean RIL population[J].Acta Agronomica Sinica,33(3):441-448.)
张富厚,郑跃进,侯典云,等.2006.大豆荚粒性状对单株产量的效应分析[J].安徽农业科学,34(15):3632-3633.(Zhang F H,Zheng Y J,Hou D Y,et al.2006.Effect of soybean pod traits on yield per plant[J].Journal of Anhui Agricultural Sciences,34(15):3632-3633.)
张维耀,王金星,宗春美,等.2012.大豆荚粒性状的QTL分析[J].大豆科学,31(2):193-197.(Zhang W Y,Wang JX,Zong C M,et al.2012.QTL analysis of seed and pod traits in soybean[J].Soybean Science,31(2):193-197.)
Arcade A,Labourdette A,Falque M,et al.2004.BioMercator:Integrating genetic maps and QTL towards discovery of candidate genes[J].Bioinformatics.20(14):2324-2326.
Chardon F,Virlon B,Moreau L,et al.2004.Genetic architecture of flowering time in maize as inferred from quantitative trait loci meta-analysis and synteny conservation with the rice genome[J].Genetics,168(4):2169-2185.
Chen Q S,Zhang Z C,Liu C Y et al.2007.QTL analysis of major agronomic traits in soybean[J].Agricultural Sciences in China,6(4):399-405.
Darvasi A,Soller M.1997.A simple method to calculate resolving power and confidence interval of QTL map location[J].Behavior Genetics,27(2):125-132.
Goffinet B,Gerber S.2000.Quantitative trait loci:A metaanalysis[J].Genetics,155(1):463-473.
Geisler D A,P?pke C,Obata T,et al.2012.Downregulation of theδ-subunit reduces mitochondrial ATP synthase levels,alters respiration,and restricts growth and gametophyte development in Arabidopsis[J].Plant Cell,24(7):2792-2811.
He J N,Duan Y,Hua D P,et al.2012.DEXH box RNA helicase-mediated mitochondrial reactive oxygen species production in Arabidopsis mediates crosstalk between abscisic acid and auxin signaling[J].Plant Cell,24(5):1815-1833.
Iwata Y J,Fedoroff N V,Koizumi N.2008.Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response[J].Plant Cell,20(11):3107-3121.
Jeong N,Suh S J,Kim M H,et al.2012.Ln is a key regulator of leaflet shape and number of seeds per pod in soybean[J].Plant Cell,24(12):4807-4818.
Ji X Y,Liu G F,Liu Y J,et al.2015.The regulatory network of ThbZIP1 in response to abscisic acid treatment[J].Frontiers in Plant Science,6(25):1-12
Kandoth P K,Ithal N,Recknor J,et al.2011.The soybean Rhg1 locus for resistance to the soybean cyst nematode heterodera glycines regulates the expression of a large number of stress-and defense-related genes in degenerating feeding cells[J].Plant Physiology,155(4):1960-1975.
Kuroda Y,Kaga A,Tomooka N,et al.2013.QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields[J].Ecology and Evolution,3(7):2150-2168.
Glass G V,Smith M L.1979.Meta-analysis of research on class size and achievement[J].Educational Evaluation&Policy Analysis,1(1):2-16.
Li,D,Sun M,Han Y,et al.(2010).Identification of QTL underlying soluble pigment content in soybean stems related to resistance to soybean white mold(Sclerotinia sclerotiorum)[J].Euphytica,172(1):49-57.
Li F L,Asami T D,Wu X Z,et al.2007.A putative hydroxysteroid dehydrogenase involved in regulating plant growth and development[J].Plant Physiology,145(1):87-97.
Liu N X,Li M,Hu X B,et al.2017.Construction of high-density genetic map and QTL mapping of yield-related and two quality traits in soybean RILs population by RAD-sequencing[J].BMC Genomics,18(1):466-478.
Liu W X,Kim M Y,Van K,et al.2011.QTL identification of yield-related traits and their association with flowering and maturity in soybean[J].Journal of Crop Science and Biotechnology,14(1):65-70.
Liu S,GuoY,Luo L,et al.2014.Integration and"Overview"analysis of QTLs related to lodging in soybean(Glycine max)[J].Journal of Plant Genetic Resources,15(1):137-143.
Liu Z,Yao D S,Zhang J,et al.2015.Identification of genes associated with the increased number of four-seed pods in soybean(Glycine max L.)using transcriptome analysis[J].Genetics and Molecular Research,14(4):18895-18912.
Merelo P,AgustíJ,Arbona V,et al.2017.Corrigendum:Cell wall remodeling in abscission zone cells during ethylene-promoted fruit abscission in citrus[J].Frontiers in Plant Science,8(126):301-320.
Murphy T M,Belmonte M,Shu S,et al.2009.Requirement for abasic endonuclease gene homologues in Arabidopsis seed development[J].PLoS One,4(1):e4297.
Mukhtar F A,Coyne D P.1981.Inheritance and association of flower,ovule,seed,pod,and maturity characters in dry edible beans(Phaseolus vulgaris L)[J].Journal American Society for Horticultural Science,106(6):713-719.
Palomeque L,Li J L,Li W B,et al.2009.QTL in mega-environments:II.agronomic trait QTL co-localized with seed yield QTL detected in a population derived from a cross of high-yielding adapted x high-yielding exotic soybean lines[J].Theoretical&Applied Genetics,119(3):429-436.
Paque S,Mouille G,Grandont L,et al.2014.Auxin binding protein1 links cell wall remodeling,auxin signaling,and cell expansion in Arabidopsis[J].Plant Cell,26(1):280-295.
Qi Z M,Huang L,Zhu R S,et al.2014.A high-density genetic map for soybean based on specific length amplified fragment sequencing[J].PLoS One,9(8):e104871.
Qi Z M,Wu Q,Han X et al.2011.Soybean oil content QTLmapping and integrating with meta-analysis method for mining genes[J].Euphytica,179(3):499-514.
Ragin B,Richard B,Willsheana C,et al.2012.Genetic analysis of yield components in the PI 438489B by'Hamilton'recombinant inbred line(RIL)population of soybean[Glycine max(L.)Merr.][J].Journal of Agricultural Science,4(9):98-105.
Shi L Y,Li X H,Hao Z F,et al.2007.Comparative QTL mapping of resistance to gray leaf spot in maize based on bioinformatics[J].Agricultural Sciences in China,6(12):1411-1419.
Sun D S,Li W B,Zhang Z C,et al.2006.Quantitative trait loci analysis for the developmental behavior of soybean(Glycine max L.Merr.)[J].Theoretical&Applied Genetics,112(4):665-673.
Swamy B M,Sarla N.2011.Meta-analysis of yield QTLs derived from inter-specific crosses of rice reveals consensus regions and candidate genes[J].Plant Molecular Biology Reporter,29(3):663-680.
Vieira A J D,Oliveira D A,Soares T C B,et al.2006.Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7and F8generations[J].Brazilian Journal of Plant Physiology,2006,18(2):281-290.
Wang X B,Zhang H W,Gao Y L,et al.2015.Evolution and association analysis of GmCYP78A10 gene with seed size/weight and pod number in soybean[J].Molecular Biology Reports,42(2):489-496.
Wang Y,Yao J,Zhang Z F,et al.2006.The comparative analysis based on maize integrated QTL map and meta-analysis of plant height QTLs[J].Chinese Science Bulletin,51(18):2219-2230.
Xu P,Wu X H,Wang B G,et al.2013.QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits.BMC Genetics,14(1):4-13.
Yang Z,Xin D W,Liu CY,et al.2013.Identification of QTLs for seed and pod traits in soybean and analysis for additive effects and epistatic effects of QTLs among multiple environments[J].Molecular Genetics and Genomics,288(12):651-667.
Zhang D,Cheng H,Wang H,et al.2010.Identification of genomic regions determining flower and pod numbers development in soybean(Glycine max L.)[J].Journal of Genetics and Genomics,37(8):545-556.
Zhang Y X,Liu S M,Dai S Y,et al.2012.Integration of shotgun proteomics and bioinformatics analysis to explore plant hormone responses[J].BMC Bioinformatics,13(15):8-18.