16S rDNA文库法分析甜菜夜蛾中肠共生菌组成
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摘要
昆虫共生菌在宿主昆虫的生长、发育及适应环境方面具有重要作用。因此,本文利用16S rDNA文库随机测序法分析了甜菜夜蛾Spodoptera exigua(Hübner)初孵幼虫和1日龄整个虫体、3龄幼虫中肠和5龄幼虫中肠的细菌菌群组成,以探究甜菜夜蛾共生菌可能存在的作用。结果显示,在甜菜夜蛾中发现了12种共生细菌,其中有1种共生细菌只能鉴定到伯克氏菌目Burkholderiales;有3种可以鉴定到科,分别是丛毛单胞菌科Comanonadaceae、肠杆菌科Enterobacteria和草酸杆菌科Massilia;剩余8种均可以鉴定到属,分别是不动杆菌属Acinetobacter、噬酸菌属Acidovorax、噬氢菌属Pelomonas、埃希氏菌属Escherichia、柠檬酸杆菌属Citrobacter、肠球菌属Enterococcus、丛毛单胞菌属Cruvibacter和肠杆菌属Enterobacter。在得到的571条有效序列中,不动杆菌属Acinetobacter相对丰度最高,占72.3%,属于优势共生菌。其次是噬氢菌属Pelomonas和噬酸菌属Acidovorax分别占12.8%和5.6%,剩余种类的相对丰度均低于5%。本研究结果证明甜菜夜蛾中存在多种共生细菌,且其种类和相对丰度随甜菜夜蛾生长发育呈动态变化。其中不动杆菌属Acinetobacter作为优势共生菌具有对多种抗生素天然耐药性的重要特征,这为研究甜菜夜蛾为何易产生较强抗药性的机理提供了新思路,也为甜菜夜蛾的微生物防治研究奠定了基础。
Insect symbiotic microbiota play essential roles in the growth,development and environmental adaptation of host insects. Therefore,the bacterial diversity and community composition in the newlyhatched larva and 1 day old、the midgut of 3 rdlarvae and 5 thlarvae of Spodoptera exigua Hübner were analyzed by 16S rDNA library random sequencing to explore the possible effect of symbiotic bacteria. The results showed that there were twelve species of symbiotic bacteria in the four different periods of S.exigua. And there was a kind of symbiotic bacteria could only identify order Burkholderiales;Comanonadaceae,Enterobacteria and Massilia could be identified family; the remaining species could be identified genus, Acinetobacter, Acidovorax, Pelomonas, Escherichia, Citrobacter, Enterococcus,Cruvibacter and Enterobacter,respectively. Among the 571 effective sequences,Acinetobacter,which belonged to the dominant symbiotic bacteria,was the highest relative abundance and accounted for 72. 3%. Followed by 12. 8% of the Pelomonas and 5. 6% of the Acidovorax,the relative abundance of the remaining bacteria were less than 5%. The results showed that there were a variety of symbiotic bacteria in the S. exigua,and with the growth development of the S. exigua,dynamic changes of species and relative abundance were observed. The dominant symbiotic bacteria of the Acinetobacter can resist various antibiotics because of his own characteristics,which provides a new idea for the study of the mechanism of strong resistance to S. exigua,and the foundation to research the microbial control of S. exigua.
Insect symbiotic microbiota play essential roles in the growth,development and environmental adaptation of host insects. Therefore,the bacterial diversity and community composition in the newlyhatched larva and 1 day old、the midgut of 3 rdlarvae and 5 thlarvae of Spodoptera exigua Hübner were analyzed by 16S rDNA library random sequencing to explore the possible effect of symbiotic bacteria. The results showed that there were twelve species of symbiotic bacteria in the four different periods of S.exigua. And there was a kind of symbiotic bacteria could only identify order Burkholderiales;Comanonadaceae,Enterobacteria and Massilia could be identified family; the remaining species could be identified genus, Acinetobacter, Acidovorax, Pelomonas, Escherichia, Citrobacter, Enterococcus,Cruvibacter and Enterobacter,respectively. Among the 571 effective sequences,Acinetobacter,which belonged to the dominant symbiotic bacteria,was the highest relative abundance and accounted for 72. 3%. Followed by 12. 8% of the Pelomonas and 5. 6% of the Acidovorax,the relative abundance of the remaining bacteria were less than 5%. The results showed that there were a variety of symbiotic bacteria in the S. exigua,and with the growth development of the S. exigua,dynamic changes of species and relative abundance were observed. The dominant symbiotic bacteria of the Acinetobacter can resist various antibiotics because of his own characteristics,which provides a new idea for the study of the mechanism of strong resistance to S. exigua,and the foundation to research the microbial control of S. exigua.
引文
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Dillon R,Charnley K.Mutualism between the desert locust Schistocerca gregaria and its gut microbiota[J].Research in Microbiology,2002,153(8):503.
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Engel P,Moran NA.The gut microbiota of insects-diversity in structure and function[J].Fems Microbiology Reviews,2013,37(5):699-735.
Fang Z,Shao J,Weng Q.De novo transcriptome analysis of Spodoptera exigua multiple nucleopolyhedrovirus(SeM NPV)genes in latently infected Se301 cells[J].Virologica Sinica,2016,31(5):425-436.
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Hongoh Y,Deevong P,Inoue T,et al.Intra-and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host[J].Applied&Environmental Microbiology,2005,71(11):6590.
Huang S,Zhang H.The impact of environmental heterogeneity and life stage on the hindgut microbiota of Holotrichia parallela larvae(Coleoptera:Scarabaeidae)[J].PLoS ONE,2013,8(2):e57169.
Huang Y,Zhan XJ,Lan JX,et al.Research progress on intestinal tract microorganism of insect[J].Hubei Agricultural Sciences,2009,48(11):2888-2890.[黄云,詹先进,蓝家样,等.昆虫肠道微生物的研究进展[J].湖北农业科学,2009,48(11):2888-2890]
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Li Q,Fan J,Sun JR,et al.Research progress in the interactions among the plants,insects and endosymbionts[J].Journal of Plant Protection,2016(6):881-891.[李迁,范佳,孙京瑞,等.昆虫内共生菌-昆虫-植物互作关系研究进展[J].植物保护学报,2016(6):881-891]
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Lu YX,Liu YQ,Li Q,et al.Research progress on intestinal microbial diversity of insects[J].Journal of Henan Agricultural Sciences,2016,45(11):1-7.[鲁迎新,刘彦群,李群,等.昆虫肠道微生物多样性研究进展[J].河南农业科学,2016,45(11):1-7]
Maidak BL,Cole JR,Lilburn TG,et al.The RDP-II(ribosomal database project)[J].Nucleic Acids Research,2001,29(1):173-174.
Oliver KM,Degnan PH,Burke GR,et al.Facultative symbionts in aphids and the horizontal transfer of ecologically important traits[J].Annual Review of Entomology,2010,55(55):247-266.
Sharon G,Segal D,Ringo JM,et al.Commensal bacteria play a role in mating preference of Drosophila melanogaster[J].Proceedings of the National Academy of Sciences,2010,107(46):20051.
Tamura K.MEGA4:Molecular evolutionary genetics analysis(MEGA)software version 4.0[J].Mol.Biol.Evol.2007,24(8):1596-1599.
Wang H.Mechanism of Carbapenems Resistance in Acinetobacter[D].Beijing:Peking Union Medical College,2005.[王辉.不动杆菌对碳青霉烯类耐药机制的研究[D].北京:中国协和医科大学,2005]
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Xia X,Zheng D,Zhong H,et al.DNA sequencing reveals the midgut microbiota of diamondback moth,Plutella xylostella(L.)and a possible relationship with insecticide resistance[J].PLoS ONE,2013,8(7):e68852.
Xiang H.Study on the Structure and Function of Intestinal Bacterial Community in Insects[D].Shanghai:Shanghai Institutes for Biological Sciences,2008.[相辉.昆虫肠道细菌群落结构与功能的研究[D].上海:中国科学院上海生命科学研究院,2008]
Xiang H,Huang YP.Symbiosis between gut microbiota and insects[J].Chinese Journal of Applied Entomology,2008,45(5):687-693.[相辉,黄勇平.肠道微生物与昆虫的共生关系[J].应用昆虫学报,2008,45(5):687-693]
Xiang H,Li MW,Zhao Y,et al.Bacterial community in midguts of the silkworm larvae estimated by PCR/DGGE and 16S rD NA gene library analysis[J].Acta Entomologica Sinica,2007,50(3):222-233.[相辉,李木旺,赵勇,等.家蚕幼虫中肠细菌群落多样性的PCR-DGGE和16SrD NA文库序列分析[J].昆虫学报,2007,50(3):222-233]
Xv G,Sun ZL,Hu XL,et al.Analysis on intestinal bacterial diversity in silkworm(Bombyx mori)based on 16S rRNA gene sequences[J].Science of Sericulture,2015(4):641-649.[许刚,孙振丽,胡小龙,等.基于16S rRNA基因序列分析家蚕肠道细菌的多样性[J].蚕业科学,2015(4):641-649]
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