食用油中三种辣椒素类物质的快速筛查
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摘要
采用稳定同位素内标与高效液相色谱-串联质谱联用技术,对食用油和地沟油中天然辣椒素、二氢辣椒素和合成辣椒素进行快速筛查。前处理采用基质分散固相萃取技术:联合了低温和中性氧化铝粉除油,除油率高达91%,大大降低了前处理时间和油脂的离子化抑制效应;并结合各自同位素内标进行定量,通过比较其保留时间,避免了假阳性结果的产生。以上3种目标物在0. 1～10 ng/mL范围内线性关系良好(r~2> 0. 995),检出限均低至0. 1μg/kg,平均回收率在88. 6%～108. 1%,相对标准偏差(RSD)均小于11%。
Three components including capsaicin,dihydrocapsaicin and nonivamide in edible oil were quickly screened by using LC-MS/MS. The samples were cleaned up by matrix dispersion solid phase extraction technique. A combination of low temperature and neutral alumina powder was used for oil removal with the oil removal rate as high as 91%,which greatly reduced the pre-processing time and inhibition effect of oil ionization. This method used internal standard to determine the quantity. The false positive results were avoided by comparing the retention time. The results indicated that the three components showed good linear relationship in the range of 0. 1-10 ng/ml( r~2> 0. 995),the average recoveries were 88. 6%-108. 1%,the relative standard deviations( RSDs,n = 3) were < 11%,and the detection limit was 0. 1 μg/kg.
Three components including capsaicin,dihydrocapsaicin and nonivamide in edible oil were quickly screened by using LC-MS/MS. The samples were cleaned up by matrix dispersion solid phase extraction technique. A combination of low temperature and neutral alumina powder was used for oil removal with the oil removal rate as high as 91%,which greatly reduced the pre-processing time and inhibition effect of oil ionization. This method used internal standard to determine the quantity. The false positive results were avoided by comparing the retention time. The results indicated that the three components showed good linear relationship in the range of 0. 1-10 ng/ml( r~2> 0. 995),the average recoveries were 88. 6%-108. 1%,the relative standard deviations( RSDs,n = 3) were < 11%,and the detection limit was 0. 1 μg/kg.
引文
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