茶树油提取物粉对小鼠免疫功能的影响
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摘要
研究茶树油提取物粉对小鼠免疫功能的影响,为茶树油产品的研发及作为畜禽养殖添加剂的应用积累资料。茶树油提取物粉对小鼠的经口急性毒性LD_(50)﹥5000 mg/kg b.w.。茶树油提取物粉分别以1250、250、50 mg/kg b.w.灌胃给予连续10 d,分别进行腹腔巨噬细胞吞噬活性和脾淋巴细胞增殖试验,测定免疫器官指数、血清溶血素水平及外周血CD3~+、CD4~+、CD8~+T淋巴细胞亚群水平。结果表明茶树油提取物粉高、中、低剂量组能极显著增加小鼠脾脏指数(P<0.01),中剂量能显著增加肝脏指数和胸腺指数(P<0.05)。中、高剂量能显著提高腹腔巨噬细胞吞噬活性和血清溶血素水平(P<0.05)。三个剂量组均能显著提高小鼠脾淋巴细胞增殖的刺激指数(P<0.05)。中剂量组能显著增加小鼠CD4+/CD8+比值(P<0.05)。因此,茶树油提取物粉在一定程度上能够提高小鼠细胞免疫和体液免疫,具有增强小鼠免疫功能的作用。
To study the effect of the tea tree oil extract powder on the immune function of mice,and to accumulate data for the research and development of the tea tree oil products and the application as additives for livestock and poultry breeding. Acute oral toxicity of the tea tree oil extract powder to mice is LD_(50)>5000 mg/kg b.w. ICR mice were orally administered at dose of 1250,250 and 50 mg/kg b. w. for 10 consecutive days respectively,the peritoneal macrophage phagocytosis activity and spleen lymphocyte proliferation test were performed respectively,and the immune organ index,serum hemolysin levels and level of CD3~+,CD4~+,CD8~+T lymphocyte subsets in peripheral blood were determined. The results showed that the high,medium and low doses of the tea tree oil extract could significantly increase the spleen index of mice( P<0.01),and the medium dose could significantly increase the liver index and thymus index( P < 0. 05). Medium and high doses of the tea tree oil extract could significantly increased the peritoneal macrophage phagocytic activity and serum hemolysin level( P < 0. 05). The stimulation index of mouse spleen lymphocyte proliferation was significantly increased in all three dose groups( P<0.05).The middle dose group significantly increased the CD4~+/CD8~+ ratio in mice( P<0.05).The conclusion is that the tea tree oil extract powder can improve the cellular immunity and humoral immunity of mice to a certain extent,and has the effect of promoting the immune function of mice.
To study the effect of the tea tree oil extract powder on the immune function of mice,and to accumulate data for the research and development of the tea tree oil products and the application as additives for livestock and poultry breeding. Acute oral toxicity of the tea tree oil extract powder to mice is LD_(50)>5000 mg/kg b.w. ICR mice were orally administered at dose of 1250,250 and 50 mg/kg b. w. for 10 consecutive days respectively,the peritoneal macrophage phagocytosis activity and spleen lymphocyte proliferation test were performed respectively,and the immune organ index,serum hemolysin levels and level of CD3~+,CD4~+,CD8~+T lymphocyte subsets in peripheral blood were determined. The results showed that the high,medium and low doses of the tea tree oil extract could significantly increase the spleen index of mice( P<0.01),and the medium dose could significantly increase the liver index and thymus index( P < 0. 05). Medium and high doses of the tea tree oil extract could significantly increased the peritoneal macrophage phagocytic activity and serum hemolysin level( P < 0. 05). The stimulation index of mouse spleen lymphocyte proliferation was significantly increased in all three dose groups( P<0.05).The middle dose group significantly increased the CD4~+/CD8~+ ratio in mice( P<0.05).The conclusion is that the tea tree oil extract powder can improve the cellular immunity and humoral immunity of mice to a certain extent,and has the effect of promoting the immune function of mice.
引文
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[7]程晓静,黄群峰,徐云龙.低聚壳聚糖复配体系对皮肤致病菌的体外抗菌活性研究[J].材料导报,2016,30(2):313-316.Cheng X J,Huang Q F,Xu Y L. Antibacterial Properties of Low Molecular-weight Chitosan Composite System Towards Skin Pathogenic Bacteria[J]. Materials Review,2016,30(2):313-316.
[8]Singh B R,Vadhana P,Bhardwaj M,et al. Comparative Antimicrobial Activity of Tea Tree Oil(Melaleuca Oil)and Common Topical Antimicrobials against Bacteria Associated With Wound and Topical Infections[J]. Pharmaceutica Analytica Acta,2017,7(11).
[9]秦文文,梁剑平,郝宝成,等.茶树油对5种常见致病菌的体外抑菌作用研究[J].黑龙江畜牧兽医,2017(21):195-199.Qin W W,Liang J P,Hao B C,et al. Bacteriostasis of Tea tree oil on five common pathogenic bacteria in vitro[J]. Heilongjiang Animal Science and Veterinary Medicine,2017(21):195-199.
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[11]Shiran S,Shlomo P,Diana K,et al. Terpinen-4-ol:A Novel and Promising Therapeutic Agent for Human Gastrointestinal Cancers[J]. Plos One,2016,11(6):e0156540.
[12]杨玉荣,郑世民,焦喜兰.益生素对雏鸡免疫器官指数的影响[J].科学技术与工程,2007,(17):4448-4450.Yang Y R,Zheng S M,Jiao X L. Effects of Probiotics on Immune Organs Index of Chicks[J]. Science Technology and Engineering,2007,(17):4448-4450.
[13]周霞,彭耀宗,黄涛,等.黄连生物碱对体外培养的小鼠腹腔巨噬细胞的影响[J].中国中药杂志,2015,(23):4660-4666.Zhou X,Peng Y Z,Huang T,et al. Effects of alkaloids from Coptidis Rhizoma on mouse peritoneal macropgages in vitro[J].China Journal of Chinese Materia Medica,2015,(23):4660-4666.
[14]周泽伟,刘浩,王凯,等.黄芪灵芝口服液对雏鸡免疫功能的影响[J].中国兽医杂志,2016,(08):59-61.Zhou Z W,Liu H,Wang K,et al. Effect of Huangqi Lingzhi Oral Liquid on Immune Function of Chicken[J]. Chinese Journal of bVeterinary Medicine,2016,(08):59-61.
[15]王思芦,汪开毓,耿毅,等.鸡枞菌多糖对小鼠T细胞免疫功能的影响[J].中国兽医科学,2013,(01):77-83.Wang S L,Wang K Y,Gen Y,et al. Effect of Termitomyces albuminosus polysaccharide on T cell immunity in mouse[J].Chinese Veterinary Science,2013,(01):77-83.
[16]吴铁,汤少明,彭剑飞,等.小鼠全血法测定血清溶血素的方法学探讨[J].中国药理学通报,1992,(02):155-156.Wu T,Tang S M,Peng J F,et al. Methodological study on determination of serum hemolysin by whole blood method in mice[J]. Chinese Pharmacological Bulletin,1992,(02):155-156.
[17]袁媛,邱霞.急性毒性试验研究进展[J].海军医学杂志,2013,(05):360-361.Yuan Y,Qiu X. Progress in acute toxicity test[J]. Journal of Navy Medicine,2013,(05):360-361.
[18]王思芦,汪开毓,赵玲,等.鸡枞菌多糖对免疫抑制小鼠免疫功能的影响[J].中国药理学通报,2013,(01):59-63.Wang S L,Wang K Y,Zhao L,et al. Effect of chicken sputum polysaccharide on immune function in immunosuppressed mice[J]. Chinese Pharmacological Bulletin,2013,(01):59-63.
[19]戴志民,王伍超.巨噬细胞吞噬作用的研究进展[J].世界最新医学信息文摘,2015(84):33-35.Dai Z M,Wang W C. Advance in Microphage Phagocytosis[J].World Latest Medicine Information,2015(84):33-35.
[20]Mantovani A,Locati M. Macrophage Metabolism Shapes Angiogenesis in Tumors[J]. Cell Metabolism,2016,24(5):653-654.
[21]Da S J L,Souza-Silva F,Pereira B A,et al. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania(Leishmania)amazonensis[J]. Biomed Research International,2017,2017(5):9089748.
[22]郭明,安高,封桂英,等. CD4+T细胞亚群在类风湿性关节炎中的研究进展[J].细胞与分子免疫学杂志,2014,30(9):1004-1007.Guo M,An G,Feng G Y. et al. Progress in Studies of CD4+Regulatory T Cells and Their Role in Rheumatoid Arthritis[J].Clinical Medical Engineering,2014,30(9):1004-1007.
[23]刘春景,白荣,宋继荣,等.蓬草除痹汤对CIA大鼠外周血T淋巴细胞亚群影响的实验研究[J].陕西中医,2014(8):1086-1087.Liu C J,Bai R,Song J R,et al. Experimental Study on Effect of Pengcao Buyu Decoction on T Lymphocyte Subsets in Peripheral Blood of CIA Rats[J]. Shanxi Journal of Traditional Chinese Medicine,2014(8):1086-1087.
[24]史钧,晏利圣.鸡红细胞免疫吸附性能与白细胞数量关系[J].生物学杂志,2005,22(2):30-31.Shi J,Yan L S. Relationship between chicken red blood cell immunoadsorption performance and white blood cell count[J].Journal of Biology. 2005,22(2):30-31.
[25]叶振南,李楠,盛丹丹,等.青钱柳多糖对高脂血症大鼠血脂及抗脂质过氧化作用的影响[J].现代食品科技,2014(4):1-5.Ye Z N,Li N,Sheng D D,et al. Effect of Cyclocarya paliurus Polysaccharide on Hyperlipidemia and Anti-lipid Peroxidation in Hyperlipidemic Rats[J]. Modern Food Science&Technology,2014(4):1-5.
[26]段淑香,韩玉生,陈刚,等.强脊康对小鼠免疫功能影响的实验研究[J].齐齐哈尔医学院学报,2011,32(3):341-342.Duan S X,Han Y S,Chen G,et al. Experimental study on the effect of Qiangkangkang on immune function in mice[J]. Journal of Qiqihar Medical College,2011,32(3):341-342.
[2]Brophy J J,Davies N W,Southwell I A,et al. Gas chromatographic quality control for oil of Melaleuca terpinen-4-ol type(Australian tea tree)[J]. Journal of Agricultural&Food Chemistry,1989,37(5):1330-1335.
[3]Pegard A. Antimicrobial activity of essential oil of Bulnesia sarmienti,Lorenz(Gaiac Wood)and of a rectified fraction of this oil.Comparison with Melaleuca alternifolia,L.(Tea Tree)essential oil activity[J]. Phytothérapie,2015,13(6):384-388.
[4]Ninomiya K,Maruyama N,Inoue S,et al. The essential oil of Melaleuca alternifolia(tea tree oil)and its main component,terpinen-4-ol protect mice from experimental oral candidiasis[J]. Biological&Pharmaceutical Bulletin,2012,35(6):861.
[5]Usachev E V,Pyankov O V,Usacheva O V,et al. Antiviral activity of tea tree and eucalyptus oil aerosol and vapour[J]. Journal of Aerosol Science,2013,59(3):22-30.
[6]Pazyar N,Yaghoobi R,Bagherani N,et al. A review of applications of tea tree oil in dermatology[J]. International Journal of Dermatology,2013,52(7):784-790.
[7]程晓静,黄群峰,徐云龙.低聚壳聚糖复配体系对皮肤致病菌的体外抗菌活性研究[J].材料导报,2016,30(2):313-316.Cheng X J,Huang Q F,Xu Y L. Antibacterial Properties of Low Molecular-weight Chitosan Composite System Towards Skin Pathogenic Bacteria[J]. Materials Review,2016,30(2):313-316.
[8]Singh B R,Vadhana P,Bhardwaj M,et al. Comparative Antimicrobial Activity of Tea Tree Oil(Melaleuca Oil)and Common Topical Antimicrobials against Bacteria Associated With Wound and Topical Infections[J]. Pharmaceutica Analytica Acta,2017,7(11).
[9]秦文文,梁剑平,郝宝成,等.茶树油对5种常见致病菌的体外抑菌作用研究[J].黑龙江畜牧兽医,2017(21):195-199.Qin W W,Liang J P,Hao B C,et al. Bacteriostasis of Tea tree oil on five common pathogenic bacteria in vitro[J]. Heilongjiang Animal Science and Veterinary Medicine,2017(21):195-199.
[10]钟昌勇,陈海燕.茶树油的应用及市场前景[J].生物质化学工程,2003,37(5):31-35.Zhong C Y,Chen H Y. Tea tree oil application and market prospects[J]. Biomass Chemical Engineering,2003,37(5):31-35.
[11]Shiran S,Shlomo P,Diana K,et al. Terpinen-4-ol:A Novel and Promising Therapeutic Agent for Human Gastrointestinal Cancers[J]. Plos One,2016,11(6):e0156540.
[12]杨玉荣,郑世民,焦喜兰.益生素对雏鸡免疫器官指数的影响[J].科学技术与工程,2007,(17):4448-4450.Yang Y R,Zheng S M,Jiao X L. Effects of Probiotics on Immune Organs Index of Chicks[J]. Science Technology and Engineering,2007,(17):4448-4450.
[13]周霞,彭耀宗,黄涛,等.黄连生物碱对体外培养的小鼠腹腔巨噬细胞的影响[J].中国中药杂志,2015,(23):4660-4666.Zhou X,Peng Y Z,Huang T,et al. Effects of alkaloids from Coptidis Rhizoma on mouse peritoneal macropgages in vitro[J].China Journal of Chinese Materia Medica,2015,(23):4660-4666.
[14]周泽伟,刘浩,王凯,等.黄芪灵芝口服液对雏鸡免疫功能的影响[J].中国兽医杂志,2016,(08):59-61.Zhou Z W,Liu H,Wang K,et al. Effect of Huangqi Lingzhi Oral Liquid on Immune Function of Chicken[J]. Chinese Journal of bVeterinary Medicine,2016,(08):59-61.
[15]王思芦,汪开毓,耿毅,等.鸡枞菌多糖对小鼠T细胞免疫功能的影响[J].中国兽医科学,2013,(01):77-83.Wang S L,Wang K Y,Gen Y,et al. Effect of Termitomyces albuminosus polysaccharide on T cell immunity in mouse[J].Chinese Veterinary Science,2013,(01):77-83.
[16]吴铁,汤少明,彭剑飞,等.小鼠全血法测定血清溶血素的方法学探讨[J].中国药理学通报,1992,(02):155-156.Wu T,Tang S M,Peng J F,et al. Methodological study on determination of serum hemolysin by whole blood method in mice[J]. Chinese Pharmacological Bulletin,1992,(02):155-156.
[17]袁媛,邱霞.急性毒性试验研究进展[J].海军医学杂志,2013,(05):360-361.Yuan Y,Qiu X. Progress in acute toxicity test[J]. Journal of Navy Medicine,2013,(05):360-361.
[18]王思芦,汪开毓,赵玲,等.鸡枞菌多糖对免疫抑制小鼠免疫功能的影响[J].中国药理学通报,2013,(01):59-63.Wang S L,Wang K Y,Zhao L,et al. Effect of chicken sputum polysaccharide on immune function in immunosuppressed mice[J]. Chinese Pharmacological Bulletin,2013,(01):59-63.
[19]戴志民,王伍超.巨噬细胞吞噬作用的研究进展[J].世界最新医学信息文摘,2015(84):33-35.Dai Z M,Wang W C. Advance in Microphage Phagocytosis[J].World Latest Medicine Information,2015(84):33-35.
[20]Mantovani A,Locati M. Macrophage Metabolism Shapes Angiogenesis in Tumors[J]. Cell Metabolism,2016,24(5):653-654.
[21]Da S J L,Souza-Silva F,Pereira B A,et al. Exploring the Association of Surface Plasmon Resonance with Recombinant MHC:Ig Hybrid Protein as a Tool for Detecting T Lymphocytes in Mice Infected with Leishmania(Leishmania)amazonensis[J]. Biomed Research International,2017,2017(5):9089748.
[22]郭明,安高,封桂英,等. CD4+T细胞亚群在类风湿性关节炎中的研究进展[J].细胞与分子免疫学杂志,2014,30(9):1004-1007.Guo M,An G,Feng G Y. et al. Progress in Studies of CD4+Regulatory T Cells and Their Role in Rheumatoid Arthritis[J].Clinical Medical Engineering,2014,30(9):1004-1007.
[23]刘春景,白荣,宋继荣,等.蓬草除痹汤对CIA大鼠外周血T淋巴细胞亚群影响的实验研究[J].陕西中医,2014(8):1086-1087.Liu C J,Bai R,Song J R,et al. Experimental Study on Effect of Pengcao Buyu Decoction on T Lymphocyte Subsets in Peripheral Blood of CIA Rats[J]. Shanxi Journal of Traditional Chinese Medicine,2014(8):1086-1087.
[24]史钧,晏利圣.鸡红细胞免疫吸附性能与白细胞数量关系[J].生物学杂志,2005,22(2):30-31.Shi J,Yan L S. Relationship between chicken red blood cell immunoadsorption performance and white blood cell count[J].Journal of Biology. 2005,22(2):30-31.
[25]叶振南,李楠,盛丹丹,等.青钱柳多糖对高脂血症大鼠血脂及抗脂质过氧化作用的影响[J].现代食品科技,2014(4):1-5.Ye Z N,Li N,Sheng D D,et al. Effect of Cyclocarya paliurus Polysaccharide on Hyperlipidemia and Anti-lipid Peroxidation in Hyperlipidemic Rats[J]. Modern Food Science&Technology,2014(4):1-5.
[26]段淑香,韩玉生,陈刚,等.强脊康对小鼠免疫功能影响的实验研究[J].齐齐哈尔医学院学报,2011,32(3):341-342.Duan S X,Han Y S,Chen G,et al. Experimental study on the effect of Qiangkangkang on immune function in mice[J]. Journal of Qiqihar Medical College,2011,32(3):341-342.