多油辣木幼苗在干旱胁迫下的生理生化响应
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摘要
通过对多油辣木幼苗设置干旱胁迫处理,测定了不同处理的生理生化指标。结果表明,在轻度和中度干旱胁迫下,幼苗叶片组织含水量随着胁迫天数的增加出现先升后降的趋势,在重度干旱胁迫下则是随着胁迫天数的增加而逐渐降低;可溶性糖含量在轻度干旱胁迫下呈现上升趋势,而中度和重度干旱胁迫强度下先增后降;可溶性蛋白的含量均呈现先升后降的趋势;游离脯氨酸含量随胁迫天数的增加呈现上升趋势;丙二醛含量在处理前期变化不大,而后期出现大幅度的增加,且会随着干旱程度的加剧而加大;相对电导率也都是随着干旱程度的加大和干旱时间的延伸而不断升高;过氧化物酶活性则是轻度干旱胁迫下呈现上升趋势,中度和重度干旱处理下都是随着时间的延伸而呈现出先增加后减少的趋势;叶绿素变化范围不大,但均低于对照组。主成分分析结果表明,辣木幼苗叶片内游离脯氨酸对其抗旱性起着重要的作用。
In this research,the physiological and biochemical indexes of different treatments were measured by different setting drought stress on Moringa oleifera seedlings.The results showed that under the mild or moderate drought stress,the water content of the leaves increased firstly and then decreased but declined continuous under the increasing time of severe drought stress.The the soluble sugar content showed an upward trend under mild drought stress,but increased firstly and then decreased under moderate and severe drought stress.The contents of soluble protein decreased firstly and then decreased under moderate and severe drought stress.The content of free proline content increased with the increase of stress days.The malondialdehyde(MDA) under the three drought stress treatments were similar at the early stage,but increased lately,and it changed rapidly with the increase of drought level.The relative conductivity was also high with the increase of drought level and the extension of drought time.The activity of peroxidase increased under mild drought stress,and chlorophyll content was decreasing under different treatments.The principal component analysis indicated that free proline in leaves of M.oleifera seedlings played the most important role in its drought resistance.
In this research,the physiological and biochemical indexes of different treatments were measured by different setting drought stress on Moringa oleifera seedlings.The results showed that under the mild or moderate drought stress,the water content of the leaves increased firstly and then decreased but declined continuous under the increasing time of severe drought stress.The the soluble sugar content showed an upward trend under mild drought stress,but increased firstly and then decreased under moderate and severe drought stress.The contents of soluble protein decreased firstly and then decreased under moderate and severe drought stress.The content of free proline content increased with the increase of stress days.The malondialdehyde(MDA) under the three drought stress treatments were similar at the early stage,but increased lately,and it changed rapidly with the increase of drought level.The relative conductivity was also high with the increase of drought level and the extension of drought time.The activity of peroxidase increased under mild drought stress,and chlorophyll content was decreasing under different treatments.The principal component analysis indicated that free proline in leaves of M.oleifera seedlings played the most important role in its drought resistance.
引文
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[12]杜春花,陆斌,陈芳,等.辣木种子发芽试验及容器苗苗木分级研究[J].西北林学院学报,2008,23(1):108-110.
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[14]刘昌芬,伍英,龙继明.不同品种和产地辣木叶片营养成分含量[J].热带农业科技,2003,26(4):1-2,14.
[15]段琼芬,李迅,陈思多,等.辣木营养价值的开发利用[J].安徽农业科学,2008,36(29):12670-12672.
[16]韦秋梅,黄毅翠,赵毅辉,等.细叶云南松幼苗对PEG-6000模拟干旱胁迫的生理响应[J].西南林业大学学报,2017,37(3):7-13.
[17]付宝春,薄伟.玉簪抗旱性隶属函数及主成分分析[J].草地学报,2014,22(6):1324-1330.
[18]石永红,万里强,刘建宁,等.多年生黑麦草抗旱性主成分及隶属函数分析[J].草地学报,2010,18(5):669-672.
[2]陈惠明.植物中的钻石—高经济价值的辣木[M].台湾:商周出版社,2003.
[3]彭兴民,郑益兴,段琼芬,等.印度传统辣木引种栽培研究[J].热带亚热带植物学报,2008,16(6):579-585.
[4]张燕平,段琼芬,苏建荣.辣木的开发与利用[J].热带农业科学,2004,24(4):42-28.
[5]马崇坚,王玉珍,任安祥,等.辣木的组织培养与快速繁殖[J].植物生理学通讯,2007,43(4): 748.
[6]Atawodi S E,Atawodi J C,Idakwo G A, et al.Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves,stem,and root rarks of Moringa oleifera Lam[J].Journal of Medicinal Food,2010,13: 710-716.
[7]Coppin J P,Yanping Xu Y P,Chen H, et al.Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera[J].Journal of Functional Foods,2013,5:1892-1899.
[8]陈少瑜,郎南军,李吉跃,等.干旱胁迫下坡柳等幼苗质膜相对透性和脯氨酸含量的变化[J].广西植物,2006,26(1):80-84.
[9]李继新,丁福章,袁有波.不同强度干旱胁迫对烤烟叶片质膜透性和丙二醛含量的影响[J].贵州农业科学,2008,36(4):34-35.
[10]吕晓静,任安祥,王羽梅.不同光照强度下辣木光合作用及与其相关生理指标的日变化[J].植物生理学通讯,2009,45(2):142-144.
[11]蒋桂芝,杨焱,龙继明,等.辣木在西双版纳栽培适应性研究[J].长江农业,2008(6):44-46.
[12]杜春花,陆斌,陈芳,等.辣木种子发芽试验及容器苗苗木分级研究[J].西北林学院学报,2008,23(1):108-110.
[13]林若冰,林仰河.辣木的丰产栽培技术[J].中国热带农业,2007(4):59-60.
[14]刘昌芬,伍英,龙继明.不同品种和产地辣木叶片营养成分含量[J].热带农业科技,2003,26(4):1-2,14.
[15]段琼芬,李迅,陈思多,等.辣木营养价值的开发利用[J].安徽农业科学,2008,36(29):12670-12672.
[16]韦秋梅,黄毅翠,赵毅辉,等.细叶云南松幼苗对PEG-6000模拟干旱胁迫的生理响应[J].西南林业大学学报,2017,37(3):7-13.
[17]付宝春,薄伟.玉簪抗旱性隶属函数及主成分分析[J].草地学报,2014,22(6):1324-1330.
[18]石永红,万里强,刘建宁,等.多年生黑麦草抗旱性主成分及隶属函数分析[J].草地学报,2010,18(5):669-672.