防感香佩包成分分析及其防治儿童上呼吸道感染作用机理研究
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摘要
目的:提取分离防感香佩包中挥发油成分,明确最佳提取条件,分析挥发性成分组成,探索防感香佩包临床疗效可能的作用机理。方法:采用水蒸气蒸馏法将37包防感香佩包进行提取分离,收集挥发油,分别对浸泡时间(1、2、3、4 h)、提取时间(1、2、3、4、5 h)、料液比(5、10、20、30倍)及提取次数(1、2、3次)进行多因素多水平考察。确定高出油率的提取分离条件,通过GC-MS对挥发油化学成分进行定性和半定量分析。结果:收集防感香佩包挥发油成分的最佳提取条件为:5倍体积水浸泡1 h,提取3次,3 h/次。挥发油中主要成分为细辛醚类(43%)和倍半萜类(50%)。结论:防感香佩包挥发油成分获得性良好,主要成分明确,其防治儿童上呼吸道感染反复发作作用机理可能与细辛醚类及倍半萜有关。
Objective:To extract and separate the volatile oil from anti-influenza sachets,clarify the optimal extraction conditions,analyze the compositions of its volatile oil, and discover the probable functional mechanism of the clinical effect of anti-influenza sachets. Methods:Extracted,separated and collected the volatile oil from 37 anti-influenza sachets by steam distillation. Multi factor and multi level study of the soaking time(1,2,3,4 h),extraction time(1,2,3,4,5 h),solid-liquid ratio(5,10,20,30 times)and extraction times(1,2,3 times) respectively. Ascertained the high-oil-yield conditions of extraction and separation. Qualitative and semi-quantitative analysis of chemical constituents of volatile oil was carried out by gas chromatography-mass spectrometry(GC-MS).Results: The optimal extraction conditions of collecting the volatile oil from anti-influenza sachets was soaking with water of 5-time volume for 1 h and extraction for 3 times, once every 3 hours. The main compositions of volatile oil were asarone(43%) and sesquiterpene(50%). Conclusion:The volatile oil of anti-influenza sachets has good availability together with clear composition,and its functional mechanism of preventing and treating recurrent upper respiratory tract infections in children may be related to asarone and sesquiterpene.
Objective:To extract and separate the volatile oil from anti-influenza sachets,clarify the optimal extraction conditions,analyze the compositions of its volatile oil, and discover the probable functional mechanism of the clinical effect of anti-influenza sachets. Methods:Extracted,separated and collected the volatile oil from 37 anti-influenza sachets by steam distillation. Multi factor and multi level study of the soaking time(1,2,3,4 h),extraction time(1,2,3,4,5 h),solid-liquid ratio(5,10,20,30 times)and extraction times(1,2,3 times) respectively. Ascertained the high-oil-yield conditions of extraction and separation. Qualitative and semi-quantitative analysis of chemical constituents of volatile oil was carried out by gas chromatography-mass spectrometry(GC-MS).Results: The optimal extraction conditions of collecting the volatile oil from anti-influenza sachets was soaking with water of 5-time volume for 1 h and extraction for 3 times, once every 3 hours. The main compositions of volatile oil were asarone(43%) and sesquiterpene(50%). Conclusion:The volatile oil of anti-influenza sachets has good availability together with clear composition,and its functional mechanism of preventing and treating recurrent upper respiratory tract infections in children may be related to asarone and sesquiterpene.
引文
[1]钱丹,黄向红,李伟明.防感香佩包预防小儿反复上呼吸道感染临床观察及药理分析[J].新中医,2014,46(7):120-122.
[2]钱丹,李穗华,徐凤霞.儿童反复上呼吸道感染发作病因分析与防治措施[J].中国医学创新,2018,15(2):94-98.
[3]李景恩,聂少平,邵灯寅,等.江香薷挥发油提取率与提取时间的动力学关系[J].食品与生物技术学报,2014,33(3):308-314.
[4]石正英,管建宏,施益农.儿童反复呼吸道感染72例致病因素分析[J].临床肺科杂志,2017,22(9):1711-1713.
[5]刁俊,朱慧华.儿童体质与中医药防治小儿反复呼吸道感染的研究进展[J].中医儿科杂志,2017,13(5):88-90.
[6]韩亚亮,刘萍,何新荣,等.石菖蒲挥发油的基本成分及其药理作用研究进展[J].中国药物应用与监测,2011,8(2):120-124.
[7]Qiu D,Hou L,Chen Y,et al.β-Asarone inhibits synaptic inputs to airway preganglionic parasympathetic motoneurons[J].Respir Physiol Neurobiol,2011,177(3):313-319.
[8]沈梅芳,李小萌,单琪媛.薄荷化学成分与药理作用研究新进展[J].中华中医药学刊,2012,30(7):1484-1487.
[9]任星宇,罗敏,邓才富,等.白芷挥发油提取方法及药理作用的研究进展[J].中国药房,2017,28(29):4167-4170.
[10]李翎,邹衍衍,石琛,等.β、α细辛醚及石菖蒲挥发油对支气管哮喘的药效对比观察[J].时珍国医国药,2006,17(11):2137-2138.
[11]张龙开,许日鑫,蒋梅,等.β-细辛醚微乳鼻腔给药脑内靶向性评价[J].中草药,2014,45(1):86-89.
[12]兰烨荣,刘素香,张铁军,等.细辛醚的研究进展[J].现代药物与临床,2013,28(2):252-257.
[13]徐静,高玲,谢永慧,等.倍半萜内酯化合物药理作用[J].中国热带医学,2007,7(4):623-624.
[2]钱丹,李穗华,徐凤霞.儿童反复上呼吸道感染发作病因分析与防治措施[J].中国医学创新,2018,15(2):94-98.
[3]李景恩,聂少平,邵灯寅,等.江香薷挥发油提取率与提取时间的动力学关系[J].食品与生物技术学报,2014,33(3):308-314.
[4]石正英,管建宏,施益农.儿童反复呼吸道感染72例致病因素分析[J].临床肺科杂志,2017,22(9):1711-1713.
[5]刁俊,朱慧华.儿童体质与中医药防治小儿反复呼吸道感染的研究进展[J].中医儿科杂志,2017,13(5):88-90.
[6]韩亚亮,刘萍,何新荣,等.石菖蒲挥发油的基本成分及其药理作用研究进展[J].中国药物应用与监测,2011,8(2):120-124.
[7]Qiu D,Hou L,Chen Y,et al.β-Asarone inhibits synaptic inputs to airway preganglionic parasympathetic motoneurons[J].Respir Physiol Neurobiol,2011,177(3):313-319.
[8]沈梅芳,李小萌,单琪媛.薄荷化学成分与药理作用研究新进展[J].中华中医药学刊,2012,30(7):1484-1487.
[9]任星宇,罗敏,邓才富,等.白芷挥发油提取方法及药理作用的研究进展[J].中国药房,2017,28(29):4167-4170.
[10]李翎,邹衍衍,石琛,等.β、α细辛醚及石菖蒲挥发油对支气管哮喘的药效对比观察[J].时珍国医国药,2006,17(11):2137-2138.
[11]张龙开,许日鑫,蒋梅,等.β-细辛醚微乳鼻腔给药脑内靶向性评价[J].中草药,2014,45(1):86-89.
[12]兰烨荣,刘素香,张铁军,等.细辛醚的研究进展[J].现代药物与临床,2013,28(2):252-257.
[13]徐静,高玲,谢永慧,等.倍半萜内酯化合物药理作用[J].中国热带医学,2007,7(4):623-624.