哈维氏弧菌致病因子检测与防治研究进展
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摘要
弧菌疾病是水产品的主要病害,哈维氏弧菌作为世界范围内近年来发现的主要海洋致病菌之一,多次引起水产品的流行性疾病,造成巨大的经济损失。本文对于哈维氏弧菌的主要致病机理以及检测技术进行了归纳,哈维氏弧菌引起宿主疾病的主要原因有哈维氏弧菌分泌的胞外产物中的溶血素(vhh),胞外蛋白酶,铁载体等,弧菌自身对于宿主血清清除作用的抗性作用以及环境因素如水温,以及菌群密度感应系统。本文还总结了对哈维氏弧菌疾病检测的防治研究进展,为今后更好的研究与预防海洋弧菌疾病提供有效的参考。
Vibriosis is the major disease of aquatic products,while Vibrio harveyi is one of the major marine pathogens discovered in recent years in the world,has repeatedly caused epidemic diseases of aquatic products,causing huge economic losses.The main pathogenic mechanism and detection technology of Vibrio harveyi and concludes the research progress on the prevention and treatment of Vibrio harveyi disease were summarized.The main cause of host disease caused by Vibrio harveyi was hemolysin (vhh),extracellular protease,iron carrier,etc.in the extracellular product secreted by Vibrio harveyi.Vibrio itself had resistance to host serum clearance. And environmental factors such as water temperature,as well as the flora density sensing system.The research progress on the prevention and treatment of Vibrio harveyi disease detection were summarized,and provide an effectived reference for better research and prevention of marine Vibrio disease in the future.
Vibriosis is the major disease of aquatic products,while Vibrio harveyi is one of the major marine pathogens discovered in recent years in the world,has repeatedly caused epidemic diseases of aquatic products,causing huge economic losses.The main pathogenic mechanism and detection technology of Vibrio harveyi and concludes the research progress on the prevention and treatment of Vibrio harveyi disease were summarized.The main cause of host disease caused by Vibrio harveyi was hemolysin (vhh),extracellular protease,iron carrier,etc.in the extracellular product secreted by Vibrio harveyi.Vibrio itself had resistance to host serum clearance. And environmental factors such as water temperature,as well as the flora density sensing system.The research progress on the prevention and treatment of Vibrio harveyi disease detection were summarized,and provide an effectived reference for better research and prevention of marine Vibrio disease in the future.
引文
[1]王刚,孙成波,蔡传彬,等.哈维氏弧菌和白斑综合症病毒对墨吉明对虾仔虾的致病性[J].热带生物学报,2016,7(1):1-9.
[2]Soto-Rodriguez S A,Gomez-Gil B,Lozano R,et al.Virulence of Vibrio harveyi responsible for the“bright-red”syndrome in the Pacific white shrimp Litopenaeus vannamei[J]. Journal of Invertebrate Pathology,2012,109(3):307-317.
[3]徐晓津,徐斌,王军,等.大黄鱼感染哈维氏弧菌后血液生化指标的变化及组织病理学观察[J].水产学报,2010,34(4):618-625.
[4]高晓建,姚东瑞,孙晶晶,等. 4株长牡蛎(Crassostrea gigas)致病性哈维氏弧菌(Vibrio harveyi)鉴定及其毒力基因检测[J].海洋湖沼通报,2015(3):87-96.
[5]沈桂明,李晨,史成银,等.哈维氏弧菌(Vibrio harveyi)ML01株胞外产物及分泌性蛋白的分离与特性分析[J].渔业科学进展,2017,38(4):25-33.
[6]张凤萍,彭志兰,张健,等.鮸鱼弧菌病病原菌(哈维氏弧菌)的分离与鉴定[J].微生物学报,2010,50(3):304-309.
[7]Hashem M,El-Barbary M.Vibrio harveyi,infection in Arabian Surgeon fish(Acanthurus sohal)of Red Sea at Hurghada,Egypt[J]. Egyptian Journal of Aquatic Research,2013,39(3):199-203.
[8]Sithigorngul P,Rukpratanporn S N,Suksawat P,et al.A simple and rapid immunochromatographic test strip for detection of pathogenic isolates of Vibrio harveyi[J].Journal of Microbiological Methods,2007,71(3):256-264.
[9]Medina A,Mancera J M,Martínez-Manzanares E,et al.Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole(Solea senegalensis,Kaup)[J].Fish and Shellfish Immunology,2015,47(1):377-380.
[10]Kumar B K,Raghunath P,Devegowda D,et al.Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood[J].International Journal of Food Microbiology,2011,145(1):244-249.
[11]Thongkao K. Establishment of immunological-based assay and molecular assay for rapid detection of Vibrio harveyi[J].Procedia-Social and Behavioral Sciences,2015,197:1627-1633.
[12]Xu X,Liu K,Wang S,et al.Identification of pathogenicity,investigation of virulent gene distribution and development of a virulent strain-specific detection PCR method for Vibrio harveyi,isolated from Hainan Province and Guangdong Province,China[J].Aquaculture,2017,468:226-234.
[13]Yi M Y,Ling L,Neogi S B,et al.Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus[J]. Food Control,2014,41(1):91-95.
[14]Ren C H,Hu C Q,Luo P,et al. Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification[J]. Microbiological Research,2009,164(5):514-521.
[15]Yu L P,Hu Y H,Zhang X H,et al.Development of a triplex loop-mediated isothermal amplification method for rapid on-site detection of three Vibrio,species associated with fish diseases[J]. Aquaculture,2013,s 414-415(2):267-273.
[16]Bai F,Sun B,Woo N,et al.Vibrio harveyi hemolysin induces ultrastructural changes and apoptosis in flounder(Paralichthys olivaceus)cells.[J]. Biochemical and Biophysical Research Communications,2010,395(1):70-75.
[17]白方方,张晓华.哈维氏弧菌密度感应系统对几种胞外酶的活性影响[J].中国海洋大学学报:自然科学版,2010,40(6):91-95.
[18]Ruwandeepika H A D,Bhowmick P P,Karunasagar I,et al.Pathogenesis,virulence factors and virulence regulation of Vibrios belonging to the Harveyi clade[J].Reviews in Aquaculture,2012,4(2):59-74.
[19]陈雅芳,王军,苏永全,等.养殖石斑鱼溃疡病病原哈维氏弧菌胞外产物的研究[J].海洋科学,2006,30(10):30-34.
[20]Zhang X H,Austin B. Haemolysins in Vibrio species[J].Journal of Applied Microbiology,2005,98(5):1011-9.
[21]王淑娴,张晓华,孙铂光,等.不同海洋弧菌中5类溶血素基因的分布及其与溶血活性和磷脂酶活性的相关性[J].中国水产科学,2007,14(4):570-578.
[22]Ferreira D,Seca A M,D C G A,et al. Targeting human pathogenic bacteria by siderophores:A proteomics review[J].Journal of Proteomics,2016,145:153-166.
[23]Kyoungmi W,Sooil P. Pathogenicity of Vibrio harveyi to cultured marine fishes in Korea[J]. Aquaculture,2008,285(1-4):8-13.
[24]Rajamani S,Sayre R T.A sensitive fluorescence reporter for monitoring quorum sensing regulated protease production in Vibrio harveyi[J]. Journal of Microbiological Methods,2011,84(2):189.
[25]Gao M,Song H,Liu X,et al. Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules[J]. Journal of Bioscience and Bioengineering,2016,121(4):406.
[26]Binns M M,Davies D L,Hardy K G.Cloned fragments of the plasmid Col V,I-K94 specifying virulence and serum resistance[J].Nature,1979,279(5716):778-781.
[27]Li M,Wang C L,Sun L.A pathogenic Vibrio harveyi lineage causes recurrent disease outbreaks in cultured Japanese flounder(Paralichthys olivaceus)and induces apoptosis in host cells[J].Aquaculture,2011,319(1):30-36.
[28]Zha S,Liu S,Su W,et al. Laboratory simulation reveals significant impacts of ocean acidification on microbial community composition and host-pathogen interactions between the blood clam and Vibrio harveyi[J].Fish and Shellfish Immunology,2017,71:393.
[29]赵吉臣,王刚,邓中宁,等.不同条件下哈维氏弧菌(Vibrio harvey)对墨吉明对虾(Fenneropenaeus merguiensis)致病性研究[J].海洋与湖沼,2015,46(6):1413-1419..
[30]钟硕良,陈月忠,周宸.环境因子对发光细菌的生长及日本对虾仔虾感染死亡率的影响[J].中国水产科学,2001,8(1):41-45.
[31]廖梅杰,张正,荣小军,等.基于vhh P2基因的SYBR Green I实时定量PCR检测哈维氏弧菌方法的建立[J].中国水产科学,2014,21(3):611-620..
[32]耿伟光,史成银,李晋,等.同步检测海水养殖动物5种病原菌的扩增子拯救多重PCR(Arm-PCR)方法的建立与应用[J].农业生物技术学报,2013,21(9):1125-1134.
[33]Cano-Gomez A,Hj L,Owens L,et al.A multiplex PCRbased protocol for identification and quantification of Vibrio harveyi-related species[J].Aquaculture,2015,437:195-200.
[34]Schikorski D,Renault T,Paillard C,et al. Development of Taq Man real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture[J]. Aquaculture,2013,392(10):106-112.
[35]Cao Y T W Z H,Jian J C,Lu Y S.Evaluation of a loopmediated isothermal amplification method for the rapid detection of Vibrio harveyi in cultured marine shellfish[J]. Letters in Applied Microbiology,2010,51(1):24-29.
[36]张静,施慧,谢建军,等.利用LAMP法快速检测致病性哈维氏弧菌[J].上海海洋大学学报,2010,19(5):601-607.
[37]Yu L,Hu Y,Zhang X,et al.Development of a triplex loopmediated isothermal amplification method for rapid on-site detection of three Vibrio species associated with fish diseases[J].Aquaculture,2013,s 414-415(2):267-273.
[38]程蝶,柴方超,蔡怡,等.环介导等温扩增联合横向流动试纸条可视化检测哈维氏弧菌的研究[J].生物技术通报,2016,32(6):60-68.
[39]Robertson P A W,Xu H S,Austin B. An enzyme-linked immunosorbent assay(ELISA)for the detection of Vibrio harveyi,in penaeid shrimp and water[J]. Journal of Microbiological Methods,1998,34(1):31-39.
[40]Kumar B K,Raghunath P,Devegowda D,et al.Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus,in seafood[J].International Journal of Food Microbiology,2011,145(1):244-249.
[41]Ng W K,Koh C B,Teoh C Y,et al. Farm-raised tiger shrimp,Penaeus monodon,fed commercial feeds with added organic acids showed enhanced nutrient utilization,immune response and resistance to Vibrio harveyi,challenge[J].Aquaculture,2015,449:69-77.
[42]杨仲明,B Vaseeharan,G S Prasad,等.大蒜对印度明对虾黑鳃病原耐药菌株哈维氏弧菌抗菌活性的研究[J].渔业信息与战略,2012,27(3):219-223..
[43]Huang H H,Liu X L,Xiang J H,et al.Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi[J]. Fish and Shellfish Immunology,2013,35(2):328-333.
[44]Pande G S J,Scheie A A,Benneche T,et al.Quorum sensing-disrupting compounds protect larvae of the giant freshwater prawn Macrobrachium rosenbergii,from Vibrio harveyi,infection[J].Aquaculture,2013,s 406-407(2):121-124.
[45]Liu X,Ji C,Zhao J,et al.Differential metabolic responses of clam Ruditapes philippinarum,to Vibrio anguillarum,and Vibrio splendidus,challenges[J]. Fish and Shellfish Immunology,2013,35(6):2001-7.
[46]Liu X,Zhao J,Wu H,et al. Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge[J]. Fish and Shellfish Immunology,2013,35(6):1969-75.
[2]Soto-Rodriguez S A,Gomez-Gil B,Lozano R,et al.Virulence of Vibrio harveyi responsible for the“bright-red”syndrome in the Pacific white shrimp Litopenaeus vannamei[J]. Journal of Invertebrate Pathology,2012,109(3):307-317.
[3]徐晓津,徐斌,王军,等.大黄鱼感染哈维氏弧菌后血液生化指标的变化及组织病理学观察[J].水产学报,2010,34(4):618-625.
[4]高晓建,姚东瑞,孙晶晶,等. 4株长牡蛎(Crassostrea gigas)致病性哈维氏弧菌(Vibrio harveyi)鉴定及其毒力基因检测[J].海洋湖沼通报,2015(3):87-96.
[5]沈桂明,李晨,史成银,等.哈维氏弧菌(Vibrio harveyi)ML01株胞外产物及分泌性蛋白的分离与特性分析[J].渔业科学进展,2017,38(4):25-33.
[6]张凤萍,彭志兰,张健,等.鮸鱼弧菌病病原菌(哈维氏弧菌)的分离与鉴定[J].微生物学报,2010,50(3):304-309.
[7]Hashem M,El-Barbary M.Vibrio harveyi,infection in Arabian Surgeon fish(Acanthurus sohal)of Red Sea at Hurghada,Egypt[J]. Egyptian Journal of Aquatic Research,2013,39(3):199-203.
[8]Sithigorngul P,Rukpratanporn S N,Suksawat P,et al.A simple and rapid immunochromatographic test strip for detection of pathogenic isolates of Vibrio harveyi[J].Journal of Microbiological Methods,2007,71(3):256-264.
[9]Medina A,Mancera J M,Martínez-Manzanares E,et al.Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole(Solea senegalensis,Kaup)[J].Fish and Shellfish Immunology,2015,47(1):377-380.
[10]Kumar B K,Raghunath P,Devegowda D,et al.Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus in seafood[J].International Journal of Food Microbiology,2011,145(1):244-249.
[11]Thongkao K. Establishment of immunological-based assay and molecular assay for rapid detection of Vibrio harveyi[J].Procedia-Social and Behavioral Sciences,2015,197:1627-1633.
[12]Xu X,Liu K,Wang S,et al.Identification of pathogenicity,investigation of virulent gene distribution and development of a virulent strain-specific detection PCR method for Vibrio harveyi,isolated from Hainan Province and Guangdong Province,China[J].Aquaculture,2017,468:226-234.
[13]Yi M Y,Ling L,Neogi S B,et al.Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus[J]. Food Control,2014,41(1):91-95.
[14]Ren C H,Hu C Q,Luo P,et al. Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification[J]. Microbiological Research,2009,164(5):514-521.
[15]Yu L P,Hu Y H,Zhang X H,et al.Development of a triplex loop-mediated isothermal amplification method for rapid on-site detection of three Vibrio,species associated with fish diseases[J]. Aquaculture,2013,s 414-415(2):267-273.
[16]Bai F,Sun B,Woo N,et al.Vibrio harveyi hemolysin induces ultrastructural changes and apoptosis in flounder(Paralichthys olivaceus)cells.[J]. Biochemical and Biophysical Research Communications,2010,395(1):70-75.
[17]白方方,张晓华.哈维氏弧菌密度感应系统对几种胞外酶的活性影响[J].中国海洋大学学报:自然科学版,2010,40(6):91-95.
[18]Ruwandeepika H A D,Bhowmick P P,Karunasagar I,et al.Pathogenesis,virulence factors and virulence regulation of Vibrios belonging to the Harveyi clade[J].Reviews in Aquaculture,2012,4(2):59-74.
[19]陈雅芳,王军,苏永全,等.养殖石斑鱼溃疡病病原哈维氏弧菌胞外产物的研究[J].海洋科学,2006,30(10):30-34.
[20]Zhang X H,Austin B. Haemolysins in Vibrio species[J].Journal of Applied Microbiology,2005,98(5):1011-9.
[21]王淑娴,张晓华,孙铂光,等.不同海洋弧菌中5类溶血素基因的分布及其与溶血活性和磷脂酶活性的相关性[J].中国水产科学,2007,14(4):570-578.
[22]Ferreira D,Seca A M,D C G A,et al. Targeting human pathogenic bacteria by siderophores:A proteomics review[J].Journal of Proteomics,2016,145:153-166.
[23]Kyoungmi W,Sooil P. Pathogenicity of Vibrio harveyi to cultured marine fishes in Korea[J]. Aquaculture,2008,285(1-4):8-13.
[24]Rajamani S,Sayre R T.A sensitive fluorescence reporter for monitoring quorum sensing regulated protease production in Vibrio harveyi[J]. Journal of Microbiological Methods,2011,84(2):189.
[25]Gao M,Song H,Liu X,et al. Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules[J]. Journal of Bioscience and Bioengineering,2016,121(4):406.
[26]Binns M M,Davies D L,Hardy K G.Cloned fragments of the plasmid Col V,I-K94 specifying virulence and serum resistance[J].Nature,1979,279(5716):778-781.
[27]Li M,Wang C L,Sun L.A pathogenic Vibrio harveyi lineage causes recurrent disease outbreaks in cultured Japanese flounder(Paralichthys olivaceus)and induces apoptosis in host cells[J].Aquaculture,2011,319(1):30-36.
[28]Zha S,Liu S,Su W,et al. Laboratory simulation reveals significant impacts of ocean acidification on microbial community composition and host-pathogen interactions between the blood clam and Vibrio harveyi[J].Fish and Shellfish Immunology,2017,71:393.
[29]赵吉臣,王刚,邓中宁,等.不同条件下哈维氏弧菌(Vibrio harvey)对墨吉明对虾(Fenneropenaeus merguiensis)致病性研究[J].海洋与湖沼,2015,46(6):1413-1419..
[30]钟硕良,陈月忠,周宸.环境因子对发光细菌的生长及日本对虾仔虾感染死亡率的影响[J].中国水产科学,2001,8(1):41-45.
[31]廖梅杰,张正,荣小军,等.基于vhh P2基因的SYBR Green I实时定量PCR检测哈维氏弧菌方法的建立[J].中国水产科学,2014,21(3):611-620..
[32]耿伟光,史成银,李晋,等.同步检测海水养殖动物5种病原菌的扩增子拯救多重PCR(Arm-PCR)方法的建立与应用[J].农业生物技术学报,2013,21(9):1125-1134.
[33]Cano-Gomez A,Hj L,Owens L,et al.A multiplex PCRbased protocol for identification and quantification of Vibrio harveyi-related species[J].Aquaculture,2015,437:195-200.
[34]Schikorski D,Renault T,Paillard C,et al. Development of Taq Man real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture[J]. Aquaculture,2013,392(10):106-112.
[35]Cao Y T W Z H,Jian J C,Lu Y S.Evaluation of a loopmediated isothermal amplification method for the rapid detection of Vibrio harveyi in cultured marine shellfish[J]. Letters in Applied Microbiology,2010,51(1):24-29.
[36]张静,施慧,谢建军,等.利用LAMP法快速检测致病性哈维氏弧菌[J].上海海洋大学学报,2010,19(5):601-607.
[37]Yu L,Hu Y,Zhang X,et al.Development of a triplex loopmediated isothermal amplification method for rapid on-site detection of three Vibrio species associated with fish diseases[J].Aquaculture,2013,s 414-415(2):267-273.
[38]程蝶,柴方超,蔡怡,等.环介导等温扩增联合横向流动试纸条可视化检测哈维氏弧菌的研究[J].生物技术通报,2016,32(6):60-68.
[39]Robertson P A W,Xu H S,Austin B. An enzyme-linked immunosorbent assay(ELISA)for the detection of Vibrio harveyi,in penaeid shrimp and water[J]. Journal of Microbiological Methods,1998,34(1):31-39.
[40]Kumar B K,Raghunath P,Devegowda D,et al.Development of monoclonal antibody based sandwich ELISA for the rapid detection of pathogenic Vibrio parahaemolyticus,in seafood[J].International Journal of Food Microbiology,2011,145(1):244-249.
[41]Ng W K,Koh C B,Teoh C Y,et al. Farm-raised tiger shrimp,Penaeus monodon,fed commercial feeds with added organic acids showed enhanced nutrient utilization,immune response and resistance to Vibrio harveyi,challenge[J].Aquaculture,2015,449:69-77.
[42]杨仲明,B Vaseeharan,G S Prasad,等.大蒜对印度明对虾黑鳃病原耐药菌株哈维氏弧菌抗菌活性的研究[J].渔业信息与战略,2012,27(3):219-223..
[43]Huang H H,Liu X L,Xiang J H,et al.Selection of Vibrio harveyi-resistant Litopenaeus vannamei via a three-round challenge selection with a pathogenic strain of V. harveyi[J]. Fish and Shellfish Immunology,2013,35(2):328-333.
[44]Pande G S J,Scheie A A,Benneche T,et al.Quorum sensing-disrupting compounds protect larvae of the giant freshwater prawn Macrobrachium rosenbergii,from Vibrio harveyi,infection[J].Aquaculture,2013,s 406-407(2):121-124.
[45]Liu X,Ji C,Zhao J,et al.Differential metabolic responses of clam Ruditapes philippinarum,to Vibrio anguillarum,and Vibrio splendidus,challenges[J]. Fish and Shellfish Immunology,2013,35(6):2001-7.
[46]Liu X,Zhao J,Wu H,et al. Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge[J]. Fish and Shellfish Immunology,2013,35(6):1969-75.