聚酰胺树脂纯化竹壳类黄酮工艺的研究
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摘要
采用聚酰胺吸附树脂对竹笋壳黄酮类化合物分离纯化,确定了聚酰胺吸附树脂对竹笋壳黄酮分离纯化的最佳工艺条件:制备5 mg/mL的竹笋壳黄酮提取液90 mL,调节pH=5,用1.8 mL/min的流速上样后,用160 mL的去离子水冲洗大量杂质,随后用120 mL的60%乙醇溶液洗脱120 mL。在此条件下,竹笋壳黄酮的纯度为58.4%,与大孔树脂纯化方法相比,该方法更具有良好的分离纯化效果。
The flavonoids from bamboo shoots were separated and purified by using polyamide adsorption resin. The optimum conditions for the separation and purification of bamboo shoot flavonoids by polyamide adsorption resin were determined as follows: preparing 5 mg/mL bamboo shoot flavonoid extraction 90 mL, adjusting pH=5, loading at flow rate of 1.8 mL/min, rinsing a large amount of impurities with 160 mL of deionized water, and then eluting 120 mL with 60% ethanol solution of 120 mL. Under these conditions, the purity of flavonoids from bamboo shoot was 58.4%, which was better than that of the macroporous resin purification method.
The flavonoids from bamboo shoots were separated and purified by using polyamide adsorption resin. The optimum conditions for the separation and purification of bamboo shoot flavonoids by polyamide adsorption resin were determined as follows: preparing 5 mg/mL bamboo shoot flavonoid extraction 90 mL, adjusting pH=5, loading at flow rate of 1.8 mL/min, rinsing a large amount of impurities with 160 mL of deionized water, and then eluting 120 mL with 60% ethanol solution of 120 mL. Under these conditions, the purity of flavonoids from bamboo shoot was 58.4%, which was better than that of the macroporous resin purification method.
引文
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[3] 陆柏益,张英,吴晓琴.竹叶黄酮的抗氧化性及其心脑血管药理活性研究进展[J].林产化学与工业,2005,25(3):120-124.
[4] 贾可敬.竹叶黄酮提取、纯化及抗氧化活性研究[D].中南林业科技大学,2014.
[5] 苏雅静,孙爱东,高雪娟.竹笋壳总黄酮提取工艺的响应面设计优化[J].食品工业科技,2010,31(1):233-236.
[6] PARK E J,JHON D Y.The antioxidant,angiotensin converting enzyme inhibition activity,and phenoLic compounds of bamboo shoot extracts[J].LWT-Food Science and TechnoLogy,2009,43(4):655-659.
[7] 陈晓侠,宋渊,张纪柏,等.吸附树脂对蛹虫草黄酮纯化工艺条件优化[J].食品科学,2015,36(4):68-73.
[8] 李夏冰,金昭君,荀航,等.竹叶总黄酮对脂肪酸合酶及人乳腺癌细胞的抑制作用[J].林产化学与工业,2017,37(5):113-118.
[9] MARTENS S,MITH?FER A.FLavones and flavone synthases[J].Phytochemistry,2005,66(20).2399-2407.
[10] 彭昕,黄亮,王平,等.雷竹笋总黄酮和总甾醇的抗氧化性与抑菌性[J].经济林研究,2017,35(3):179-185.
[11] BERETZ A,STIERLE A.Flavonoids and Bioflavoniods[M].Oxford:Elsevier,1981:421-425.
[12] 方小燕.聚酰胺树脂在分离纯化黄酮中的应用研究[J].海峡药学,2013,25(5):41-42.
[13] 陈帅,王慧竹,王冠,等.聚酰胺树脂分离纯化罗布麻叶总黄酮的工艺研究[J].河南工业大学学报(自然科学版),2016,37(2):109-116.
[14] 王文文,袁艺,袁彩红,等.毛竹笋总黄酮的提取工艺研究[J].安徽农业大学学报,2011,38(2):197-201.
[15] 苏雅静.竹笋壳黄酮的高效提取及其对酪氨酸酶活性影响的动力学研究[D].北京林业大学,2010.
[16] 杨乐,王洪新.笋壳黄酮分离纯化工艺及其抗氧化性[J].食品与发酵工业,2010,36(8):184-189.
[17] 王梅霞,陈双林,霍娟.一株银杏内生真菌的分离及其产黄酮类物质的初步研究[J].工业微生物,2004,34 (2):15-18.