荧光分光光度法测定钙制剂中微量铝(Ⅲ)
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摘要
以8-羟基喹啉为络合剂,采用荧光分光光度法测定钙制剂中微量铝(Ⅲ),考察铝(Ⅲ)与8-羟基喹啉的显色条件.在pH=6. 0的醋酸-醋酸铵缓冲溶液中,铝(Ⅲ)与8-羟基喹啉形成稳定的配合物.使用三氯甲烷萃取,配合物的激发波长和发射波长分别为392 nm和512 nm.铝(Ⅲ)在2～200μg/L范围内,荧光强度与铝(Ⅲ)含量具有良好的线性关系,相关系数R~2=0. 998 3;方法的最低检出限为0. 2μg/L.该方法准确,简便,灵敏度高,可用于钙试剂中铝(Ⅲ)的测定.
In this paper,8-hydroxyquinoline as complexing agent and trichloromethane as extracting solvent were used to determine trace aluminium(Ⅲ) in calcium reagent by fluorescence spectrophotometry.The chromogenic conditions of aluminum(Ⅲ) and 8-hydroxyquinoline were investigated. In the acetic acid-ammonium acetate buffer solution of pH = 6. 0,stable complexes was formed by aluminum(Ⅲ) and 8-hydroxyquinolinol. Chloroform was used as extraction agent. The excitation and emission wavelengths of complexes were 392 nm and 512 nm,respectively. The fluorescence intensity of aluminum(Ⅲ) has a good linear relationship with the content of aluminum(Ⅲ) in the range of 2 ～ 200 g/L. The correlation coefficient R~2= 0. 998 3. The method is accurate,simple and sensitive,and it can be used for the determination of aluminum(Ⅲ) in calcium preparations.
In this paper,8-hydroxyquinoline as complexing agent and trichloromethane as extracting solvent were used to determine trace aluminium(Ⅲ) in calcium reagent by fluorescence spectrophotometry.The chromogenic conditions of aluminum(Ⅲ) and 8-hydroxyquinoline were investigated. In the acetic acid-ammonium acetate buffer solution of pH = 6. 0,stable complexes was formed by aluminum(Ⅲ) and 8-hydroxyquinolinol. Chloroform was used as extraction agent. The excitation and emission wavelengths of complexes were 392 nm and 512 nm,respectively. The fluorescence intensity of aluminum(Ⅲ) has a good linear relationship with the content of aluminum(Ⅲ) in the range of 2 ～ 200 g/L. The correlation coefficient R~2= 0. 998 3. The method is accurate,simple and sensitive,and it can be used for the determination of aluminum(Ⅲ) in calcium preparations.
引文
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[2]陈建军,杨双喜,杨庆荣,等.铝对人类健康的影响及相关食品安全问题研究进展[J].中国卫生检验杂志,2007,17(7):1326-1329.
[3] SORENSON J R J,CAMPBELL I R,TEPPER L B,et al. Aluminum in the Environment and Human Health[J]. Environ Health Perspect,1974,8:3-95.
[4]张慧敏,刘桂华,罗若荣,等.食品中铝含量测定方法的研究[J].食品安全质量检测学报,2014(10):3223-3229.
[5] NANDA B B,BISWAL R R,ACHARYA R,et al.Determination of Aluminium Contents in Selected Food Samples by Instrumental Neutron Activation Analysis[J]. Journal of Radioanalytical and Nuclear Chemistry,2014,302(3):1471-1474.
[6] KHANHUATHON Y, SIRIANGKHAWUT W,CHANTIRATIKUL P,et al. Spectrophotometric M ethod for Determination of Aluminium Content inWater and Beverage Samples Employing FlowBatch Sequential Injection System[J]. Journal of Food Composition and Analysis,2015,41:45-53.
[7]中华人民共和国卫生部,中国国家标准化管理委员会. GB/T 5009. 182—2003面制食品中铝的测定[S].北京:中国标准出版社,2004:9.
[8]李志娟,强立新,宋春伟,等.铬天青S分光光度法与水杨基荧光酮-氯代十六烷基吡啶分光光度法测定饮用水中铝的比对试验[J].华北煤炭医学院学报,2005,7(3):279-280.
[9]叶耀东,刘振华,骆胜超.改进铬天青S光度法测定饮用天然矿泉水中铝[J].饮料工业,2009,12(6):38-40.
[10]张涛. 8-羟基喹啉分光光度法测定铍铜中间合金中铝[J].冶金分析,2013,33(3):60-64.
[11]程代,朱春秋,许慧玲,等. 8-羟基喹啉荧光分光光度法测定油炸食品中的铝[J].中国食品卫生杂志,2011,23(4):317-319.