当归饮子急性毒性实验研究
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摘要
目的:观察当归饮子急性毒性反应和动物死亡情况,测定药物的LD_(50),为临床应用的安全性提供试验依据。方法:采用改良寇氏法测定当归饮子对大鼠的半数致死量(LD_(50))。结果:通过急性毒性预实验和正式实验未能测出当归饮子LD_(50),大鼠急性毒性实验体质量及组织病理切片,无明显病理变化。结论:当归饮子具有较好的安全性,临床用药安全可靠。
Objective:To observe the acute toxicity and animal mortality of Angelica Sinensis and determine the LD_(50) of the drug and provide the experimental basis for the safety of clinical application.Methods:The modified KOU's method was used to determine the median lethal dose(LD_(50)) of Angelica Sinensis.Results:The acute toxicity of the experimental body and the histopathological biopsy of the acute toxicity test were not detected by acute toxicity preoperative experiment or formal experiment.There was no obvious pathological change.Conclusion:Angelica has a good,and safe and reliable effect.
Objective:To observe the acute toxicity and animal mortality of Angelica Sinensis and determine the LD_(50) of the drug and provide the experimental basis for the safety of clinical application.Methods:The modified KOU's method was used to determine the median lethal dose(LD_(50)) of Angelica Sinensis.Results:The acute toxicity of the experimental body and the histopathological biopsy of the acute toxicity test were not detected by acute toxicity preoperative experiment or formal experiment.There was no obvious pathological change.Conclusion:Angelica has a good,and safe and reliable effect.
引文
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[2] 邓中甲,主编.方剂学[M].北京:中国中医药出版社,2010.
[3] 郭静,杜艾嫒,余倩颖,等.当归饮子加减方治疗慢性荨麻疹的临床观察[J].广州中医药大学学报,2015,32(2):216-219.
[4] OECD.Acute oral toxicity-fixed dose procedure[J].OECD guideline for testing of chemicals,2001.
[5] 石春蕊,石春波,匡钱华,等.当归饮子治疗慢性荨麻疹疗效与安全性的系统评价[J].中国循证医学杂志,2012,12(10):1261-1269.
[6] 张菡,王莉.当归饮子加减治疗老年性皮肤瘙痒症80例[J].山东中医杂志,2012,31(7):492-493.
[7] 李芳梅,杨志波,曾宪玉.当归饮子对寻常型银屑病患者皮肤屏障功能的影响[J].中医药导报,2014,20(7):11-13.
[8] 王进恩,祝存海,叶昶.当归饮子加味内服外用三黄油剂治疗慢性阴囊湿疹96例临床分析[J].临床医学工程,2012,19(1):71-72.
[9] 高学敏.中药学[M].北京:中国中医药出版社,2007:433.
[10] 王栩芮,周策,钟振东,等.当归饮子加减方对气血两虚模型小鼠迟发型超敏反应的影响[J].中国中西医结合杂志,2016,36(3):345-347.
[11] 郭静,肖敏,左小红,等.基于久病入络理论的当归饮子通过调控PIP2/IP3/DAG信号通路对荨麻疹的防治及其机理研究[J].时珍国医国药,2015,26(10):2333-2335.