圆孢蘑菇中抑菌活性物质的提取分离与含量测定
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摘要
为研究圆孢蘑菇中具有抑菌活性的小分子化合物,建立圆孢蘑菇中其含量的测定方法。采用硅胶柱色谱、Sephadex LH-20和高效液相色谱等分离纯化技术,结合波谱数据分离出圆孢蘑菇中具有抑菌活性的小分子化合物没食子酸,同时利用HPLC法测定其在圆孢蘑菇中的含量为0.566 4 mg·g~(-1)。此方法简便、准确,可用于圆孢蘑菇的质量控制。
To study the antibacterial activity of small molecular compounds in the Agaricus gennadii,a method for the determination of its content has been established in this work. Separation and purification techniques such as silica gel column chromatography,Sephadex LH-20 and high performance liquid chromatography were employed to isolate gallic acid and other small molecular compounds. Enperimanfal results show that the content of gallic acid with antibacterial activity was 0.566 4 mg·g~(-1) in the Agaricus gennadi. This method is simple and accurate and thus it can be used for the purpose of quality control for Agaricus gennadi.
To study the antibacterial activity of small molecular compounds in the Agaricus gennadii,a method for the determination of its content has been established in this work. Separation and purification techniques such as silica gel column chromatography,Sephadex LH-20 and high performance liquid chromatography were employed to isolate gallic acid and other small molecular compounds. Enperimanfal results show that the content of gallic acid with antibacterial activity was 0.566 4 mg·g~(-1) in the Agaricus gennadi. This method is simple and accurate and thus it can be used for the purpose of quality control for Agaricus gennadi.
引文
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[3]岳乐乐,汪晓,张莲珠.松茸研究进展[J].特产研究,2017,39(3):72-76.
[4]GONCLVES J L,ROMA E H,GOMES S A C,et al.Proinflammatory effects of the mushroom Agaricus blazei and its consequences on atherosclerosis development[J].Eur J Nutr,2012,51(8):927-337.
[5]KIM S S,LEE J S,CHO J Y,et al.Process development for mycelial growth and polysaccharide production in Tricholoma matsutake liquid culture[J].J Biosci Bioeng,2010(109):351-355.
[6]DING X,TANGA J,CAO M,et al.Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake[J].Int J Biol Macromol,2010(47):271-275.
[7]陈诚,李强,黄文丽.松茸多糖提取方法、结构分析及生物活性研究进展[J].天然产物研究与开发,2016,28(9):1499-1503,1426.
[8]MANACH C,WILLIAMSON G,MORAND C,et al.Bioavailability and bioefficacy of polyphenols in humans.I.Review of 97bioavailability studies[J].Am J Clin Nutr,2005,81(1):230-242.
[9]LIU H B,ZHENG A M,LIU H L,et al.Identification of three novel polyphenolic compounds,origanine A-C,with unique skeleton from Origanum vulgare L.using the hyphenated LC-DAD-SPE-NMR/MS methods[J].J Agr Food Chem,2012,60(1):129-135.
[10]张丽,冯锋,柳文媛.HPLC法测定不同产地虎耳草中没食子酸、原儿茶酸和虎耳草素的含量[J].西北药学杂志,2011,26(2):88-90.
[11]李骅,谢艳华,张邦乐.RP-HPLC法同时测定双丹口服液中没食子酸、丹参素和原儿茶醛的含量[J].中国医药导报,2012,9(5):117-118,122.
[12]常连举,张宗和,黄嘉玲,等.没食子酸的制备与应用综述[J].生物质化学工程,2010,44(4):48-52.
[13]何丽清,佘志刚.五倍子染料中没食子酸的分子结构鉴定与性能研究[J].化纤与纺织技术,2017,46(2):5-9.
[14]柳贤福,黄红泓,覃日宏,等.白苞蒿提取工艺优化及槲皮素含量测定[J].湖南师范大学自然科学学报,2018,41(5):43-47.
[15]王秋萍,何珺,马海霞,等.HPLC-RID同时测定艾纳香叶中左旋樟脑、左旋龙脑及异龙脑含量[J].中华中医药杂志,2017,32(3):1267-1270.
[16]刘丽艳.分心木中没食子酸的提取工艺研究[J].广东化工,2018,45(2):42-43.
[17]李忠琴,林茂,赖晓健,等.高效液相色谱法检测优化诃子没食子酸萃取工艺[J].西华大学学报(自然科学版),2016,35(5):34-38.
[18]许维国,刘洋,刘多见,等.没食子酸抑菌活性分析[J].中国公共卫生,2012,28(10):1329-1331.
[19]王春娟,左国营,王根春.没食子酸与5种抗生素联用对铜绿假单胞菌的体外抑菌活性[J].中国药房,2014,25(1):25-27.
[20]张雅丽,李建科,刘柳.没食子酸的体外抑菌作用研究[J].食品工业科技,2013,34(11):81-84.