消糜栓对霉菌性、滴虫性阴道炎的作用研究
摘要
目的:探讨消糜栓对霉菌性阴道炎和滴虫性阴道炎的治疗作用.方法:(1)通过体外抑菌试验,测定对白色念珠菌的最低杀菌浓度(MBC).(2)通过摘除大鼠卵巢,皮下注射苯甲酸雌二醇及氢化可的松建立大鼠假动情及免疫低下所致的霉菌感染性阴道炎模型,观察消糜栓的体内抑菌作用.(3)通过消糜栓体外、体内抗阴道滴虫实验,观察消糜栓对滴虫的抑制作用.结果:(1)消糜栓对白色念珠菌的最低杀菌浓度(MBC)为37.5mg·m L~(-1).(2)霉菌性阴道炎实验中,消糜栓高、中剂量组(540、270mg·kg~(-1))菌落个数在10个以下,具有显效作用,而消糜栓低剂量组(135mg·kg~(-1))的菌落个数在11~30个区间,判定为有效作用.与模型组比较,消糜栓高、中、低剂量组(540、270、135mg·kg-1)能明显降低大鼠血清中肿瘤坏死因子TNF-α水平(P<0.01,P<0.05).(3)消糜栓体内滴虫实验,消糜栓高、中、低剂量组(780、360、180mg·kg-1)滴虫数明显少于模型组(P<0.01,P<0.05).结论:消糜栓对霉菌性、滴虫性阴道炎及表现症状有显著治疗作用.
引文
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[2]彭仁菊.中西药外洗相结合治疗霉菌性阴道炎50例[J].中医药临床杂志,2013,25(6):485.
[3]宁艳萍.口服联合外用药物治疗念珠菌性外阴阴道炎患者的临床效果[J].中国药物与临床,2014,14(5):649-650.
[4]何义萍.霉菌性阴道炎的危险因素分析[J].中国医药指南,2013,11(10):605-606.
[5]徐淑云.药理实验方法学[M].第三版.北京:人民卫生出版社,2002:1661-1663.
[6]夏德超,丁娟,吴艳,等.白色念珠菌阴道炎鼠模型构建的研究[J].中国麻风皮肤病杂志,2006,22(6):441.
[7]吕红.奥硝唑凝胶剂体内外抗阴道毛滴虫作用[J].中国医院药学杂志,2010,30(14):1203-1206.
[8]Chami F,Chami N,Bennis S,et al.Evaluation of carvacrol and eugenol as prophrlaxis and treatment of vaginal candidiasis in an immunosuppressed rat model[J].Journal of Antimicrobial Chemotherapy 2004,54∶909-914.
[9]Cherpes T L,W iesenfeld H C,Melan M A,et al.The associations between pelvic inflammatory disease,Trichomonas vaginalis infection,and positive herpes simplex virus type 2 serology[J].Sex Transm Dis,2006,33:747-752.
[10]Soper D E,Bump R C,Hurt W G.Bacterial vaginosis and trichomonas vaginitis are risk factors for cuff cellulitis after abdominal hysterectomy[J].Am J Obstet Gyneco,1990,163:1016-1023.
[11]Minkoff H,Grunebaum A N,Schwarz R H,et al.Risk factors forprematurity and premature rupture ofmembranes:a prospective study of the vaginal flora in pregnancy[J].Am J Obstet Gyneco,1984,150:965-972.
[12]郭红宇.女性阴道炎患者阴道内环境中氧化应激状况及其致细胞凋亡的机制研究[D].兰州:兰州大学,2012.
[13]于东平,杨爱英,李云峰.苦参洗剂的药理作用研究[J].山东医药工业,2002,21(5):47-49.