摘要
目的制备活血散结方含药血浆,并研究其不同浓度对视网膜色素上皮(retinal pigment epithelium,RPE)细胞的毒性作用。方法以成年健康家兔为对象,制备活血散结方含药血浆。将提取的兔原代RPE细胞分9个组,分别为:空白对照组、正常血浆组、5%含药血浆组、10%含药血浆组、20%含药血浆组、40%含药血浆组、60%含药血浆组、80%含药血浆组、100%含药血浆组,予以相对应浓度的含药血浆干预,CCK8法检测含药血浆对兔RPE细胞毒作用。结果活血散结方各浓度含药血浆对RPE细胞有抑制作用,其中5%~20%含药血浆对RPE细胞有较弱的抑制作用,但与正常血浆比较,抑制率差异无统计学意义(P>0.05);而40%~100%含药血浆对RPE细胞生长有明显的抑制作用,与正常血浆比较,抑制率差异有统计学意义(P<0.05)。结论活血散结方含药血浆对RPE细胞有一定的抑制作用,抑制程度与含药血浆浓度成量效关系,可以考虑选择5%~20%含药血浆浓度作为后续实验研究的干预浓度。
Objective To prepare Huoxue Sanjie Decoction-containing plasma, and to study the toxic effect of different concentrations of the plasma on retinal pigment epithelium(RPE) cells. Methods Adult healthy rabbits were used to prepare Huoxue Sanjie Decoction-containing plasma. Rabbit primary cells were divided into nine groups: blank control group, normal plasma group, 5% drug-containing plasma group, 10% drug-containing plasma group, 20% drug-containing plasma group, 40%drug-containing plasma group, 60% drug-containing plasma group, 80% drug-containing plasma group, and 100% drug-containing plasma group. These groups were treated with respective concentrations of drug-containing plasma. CCK-8 assay was used to examine their cytotoxic effect on rabbit RPE cells. Results All concentrations of Huoxue Sanjie Decoction-containing plasma had an inhibitory effect on RPE cells. The 5%, 10%, and 20% drug-containing plasma had a weak inhibitory effect on RPE cells,which was not significantly different from the inhibitory effect of normal plasma(P>0.05). However, the 40%, 60%, 80%, and 100%drug-containing plasma had an obvious inhibitory effect on RPE cell growth, and the inhibition rates were significantly from that of the normal plasma group(P<0.05). Conclusion Huoxue Sanjie Decoction-containing plasma has a certain inhibitory effect on RPE cells, which is correlated with the concentration of drug-containing plasma. The 5%-10% concentration of drug-containing plasma can be considered as the intervention concentration in the following experimental studies.
引文
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