不同品种工业大麻中大麻二酚含量分析
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摘要
建立使用HPLC测不同品种工业大麻中大麻二酚含量的分析方法;在Eclipse Plus C18 (150 mm×4.6 mm,5μm)色谱柱,水-甲醇-乙腈为流动相梯度洗脱,流速为1.0 mL/min,检测波长210 nm,柱温30℃的色谱条件下测不同品种工业大麻中大麻二酚的含量。结果表明,大麻二酚在质量浓度0.0675～0.5400μg/μL (R2=0.9927)范围内有良好的线性关系,加样回收率为101.3%(RSD=2.51%)。该方法操作简便,具有良好的重复性和回收率,适用于工业大麻中大麻二酚的性量分析。
A method for the determination of cannabinol in different varieties of hemp by HPLC was established.Under the chromatographic conditions of Eclipse Plus C18(150 mm×4.6 mm,5 μm)column with water-methanolacetonitrile as mobile phase gradient elution, flow rate of 1.0 mL/min, detection wavelength of 210 nm and column temperature of 30℃, the content of cannabinol in different kinds of industrial cannabis was determined. The results showed that cannabinol had a good linear relationship in the range of 0.0675-0.5400 μg/μL(R2 = 0.9927), and the recovery of cannabinol was 101.3%(RSD=2.51%). The method is simple, reproducible and has good recovery. It is suitable for the qualitative and quantitative analysis of cannabinol in industrial hemp.
A method for the determination of cannabinol in different varieties of hemp by HPLC was established.Under the chromatographic conditions of Eclipse Plus C18(150 mm×4.6 mm,5 μm)column with water-methanolacetonitrile as mobile phase gradient elution, flow rate of 1.0 mL/min, detection wavelength of 210 nm and column temperature of 30℃, the content of cannabinol in different kinds of industrial cannabis was determined. The results showed that cannabinol had a good linear relationship in the range of 0.0675-0.5400 μg/μL(R2 = 0.9927), and the recovery of cannabinol was 101.3%(RSD=2.51%). The method is simple, reproducible and has good recovery. It is suitable for the qualitative and quantitative analysis of cannabinol in industrial hemp.
引文
[1]高哲,张志军,李晓君,等.火麻叶中大麻二酚的热回流法提取工艺研究[J].中国油脂,2019(03):107-111
[2] Zuardi A W, Crippa J A S, Hallak J E C, et al. Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug[J]. Brazilian Journal of Medical and Biological Research, 2006,39(04):421-429
[3] Danilo D G, Mclaughlin R J, Luca P, et al. Cannabidiol modulates serotonergic transmission and prevents allodynia and anxiety-like behavior in a model of neuropathic pain[J]. PAIN, 2019,160:136-150
[4] Mechoulam R, Parker L A, Gallily R. Cannabidiol:An Overview of Some Pharmacological Aspects[J]. The Journal of Clinical Pharmacology, 2002, 42(Z1):11-19
[5] Devinsky O, Cross J H, Laux L, et al. Trial of Cannabidiol for Drug-Resistant Seizures in the Dravet Syndrome[J]. New England Journal of Medicine, 2017,376(21):2011-2020
[6] Attard T M, Bainier C, Reinaud M, et al. Utilisation of supercritical fluids for the effective extraction of waxes and Cannabidiol(CBD)from hemp wastes[J]. Industrial Crops and Products,2018,112:38-46
[7] Cuba L F, Salum F G, Cherubini K, et al. Cannabidiol:an alternative therapeutic agent for oral mucositis?[J]. Journal of Clinical Pharmacy and Therapeutics, 2017,42:245-250
[8] Lodzki M, Godin B, Rakou L, et al. Cannabidiol—transdermal delivery and anti-inflammatory effect in a murine model[J]. Journal of Controlled Release, 2003,93(3):377-387
[9] Ambach L, Penitschka F, Broillet A, et al. Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD[J]. Forensic Science International, 2014,243:107-111
[10] Brighenti V, Pellati F, Steinbach M, et al. Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type, Cannabis sativa, L.(hemp)[J]. Journal of Pharmaceutical and Biomedical Analysis,2017,143:228-236
[11]高宝昌,孙宇峰,张旭,等.工业大麻叶中大麻二酚含量分析研究[J].黑龙江科学,2018, 9(01):61-63
[12]张旭,孙宇峰,田媛,等. SPE-HPLC法同时测汉麻提取物中3种大麻酚含量[J].化学试剂, 2018, 40(06):547-550
[2] Zuardi A W, Crippa J A S, Hallak J E C, et al. Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug[J]. Brazilian Journal of Medical and Biological Research, 2006,39(04):421-429
[3] Danilo D G, Mclaughlin R J, Luca P, et al. Cannabidiol modulates serotonergic transmission and prevents allodynia and anxiety-like behavior in a model of neuropathic pain[J]. PAIN, 2019,160:136-150
[4] Mechoulam R, Parker L A, Gallily R. Cannabidiol:An Overview of Some Pharmacological Aspects[J]. The Journal of Clinical Pharmacology, 2002, 42(Z1):11-19
[5] Devinsky O, Cross J H, Laux L, et al. Trial of Cannabidiol for Drug-Resistant Seizures in the Dravet Syndrome[J]. New England Journal of Medicine, 2017,376(21):2011-2020
[6] Attard T M, Bainier C, Reinaud M, et al. Utilisation of supercritical fluids for the effective extraction of waxes and Cannabidiol(CBD)from hemp wastes[J]. Industrial Crops and Products,2018,112:38-46
[7] Cuba L F, Salum F G, Cherubini K, et al. Cannabidiol:an alternative therapeutic agent for oral mucositis?[J]. Journal of Clinical Pharmacy and Therapeutics, 2017,42:245-250
[8] Lodzki M, Godin B, Rakou L, et al. Cannabidiol—transdermal delivery and anti-inflammatory effect in a murine model[J]. Journal of Controlled Release, 2003,93(3):377-387
[9] Ambach L, Penitschka F, Broillet A, et al. Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD[J]. Forensic Science International, 2014,243:107-111
[10] Brighenti V, Pellati F, Steinbach M, et al. Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type, Cannabis sativa, L.(hemp)[J]. Journal of Pharmaceutical and Biomedical Analysis,2017,143:228-236
[11]高宝昌,孙宇峰,张旭,等.工业大麻叶中大麻二酚含量分析研究[J].黑龙江科学,2018, 9(01):61-63
[12]张旭,孙宇峰,田媛,等. SPE-HPLC法同时测汉麻提取物中3种大麻酚含量[J].化学试剂, 2018, 40(06):547-550