亚热带典型岩溶水库细菌丰度和细菌生产力及其与环境因子的关系
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摘要
细菌丰度是反映水体污染状况的敏感指标.为了解亚热带岩溶区水库营养状况,细菌丰度和细菌生产力及其与环境因子的关系,本文以亚热带典型岩溶水库——广西上林县大龙洞水库为研究对象,采用综合营养状态指数法(TLI)评价大龙洞水库水体的营养化类型、荧光定量PCR技术研究细菌丰度分布情况以及14C标记法测定细菌生产力.结果表明,大龙洞水库处于富营养化状态,细菌丰度在调查水域存在明显空间异质性;沿着水流方向从上游到下游表层水的细菌丰度呈先递减后增加的趋势;细菌丰度垂向分布特征均表现为表层>中层>底层;细菌生产力的变化特征为表层水的细菌生产力明显高于中层和底层.相关性分析表明,细菌丰度与细菌生产力呈显著正相关;细菌丰度与温度、p H、高锰酸盐指数呈显著正相关,与电导率和DIC呈显著负相关,与DO、叶绿素a、DOC呈极显著正相关;细菌生产力则与p H、DOC、高锰酸盐指数呈显著正相关,与电导率、DIC呈显著负相关,与DO极显著正相关.主成分分析将影响细菌丰度和细菌生产力的10个环境因子均划分为两个主成分,第一主成分为温度、p H、电导率、DIC、DO、Chla、DOC和高锰酸盐指数,第二主成分为TN和TP,表明大龙洞水库细菌丰度和细菌生产力受多种环境因子共同影响,进而使光合细菌成为水体初级生产力的重要贡献者.
In recent years,the increasing scarcity of water resources and eutrophication of water have become more serious. Reservoirs that are far from big cities have become important sources of drinking water and were targets of research and protection. Therefore,the abundance and production of bacteria and their correlations with environmental factors were investigated in the Dalongdong Reservoir,Shanglin County,Guangxi Province,using Quantitative Real-time PCR technology and the14 C tracer technique. The Dalongdong Reservoir is a typical subtropical karst reservoir. The bacterial abundance of the surface water decreased from the upstream to the downstream along the water flow direction and then increases. The vertical distribution of the bacterial abundance at each sampling site shows a similar trend; it is the highest in the surface water and the lowest on the bottom. The correlation analysis results show that the temperature,p H,electrical conductivity,dissolved organic carbon(DOC),chlorophyll-a,dissolved oxygen(DO),and other environmental factors significantly correlate with the bacterial abundance,indicating that these parameters are the main factors limiting the bacterial abundance in this region. The bacterial production is positively correlated with the p H,DOC,and permanganate index;negatively correlated with the conductivity and DIC; and significantly positively correlated with DO. The principal component analyses(PCA) shows that the environmental factors affecting the bacterial abundance and bacterial production can be grouped into two PCAs.PCA1 includes the temperature,p H,electrical conductivity,DIC,DO,chlorophyll-a,DOC,and permanganate index and PCA2 includes TN and TP. The bacterial abundance and production in the Dalongdong Reservoir are affected by various environmental factors and photosynthetic bacteria are the important contributors to the production of organic carbon.
In recent years,the increasing scarcity of water resources and eutrophication of water have become more serious. Reservoirs that are far from big cities have become important sources of drinking water and were targets of research and protection. Therefore,the abundance and production of bacteria and their correlations with environmental factors were investigated in the Dalongdong Reservoir,Shanglin County,Guangxi Province,using Quantitative Real-time PCR technology and the14 C tracer technique. The Dalongdong Reservoir is a typical subtropical karst reservoir. The bacterial abundance of the surface water decreased from the upstream to the downstream along the water flow direction and then increases. The vertical distribution of the bacterial abundance at each sampling site shows a similar trend; it is the highest in the surface water and the lowest on the bottom. The correlation analysis results show that the temperature,p H,electrical conductivity,dissolved organic carbon(DOC),chlorophyll-a,dissolved oxygen(DO),and other environmental factors significantly correlate with the bacterial abundance,indicating that these parameters are the main factors limiting the bacterial abundance in this region. The bacterial production is positively correlated with the p H,DOC,and permanganate index;negatively correlated with the conductivity and DIC; and significantly positively correlated with DO. The principal component analyses(PCA) shows that the environmental factors affecting the bacterial abundance and bacterial production can be grouped into two PCAs.PCA1 includes the temperature,p H,electrical conductivity,DIC,DO,chlorophyll-a,DOC,and permanganate index and PCA2 includes TN and TP. The bacterial abundance and production in the Dalongdong Reservoir are affected by various environmental factors and photosynthetic bacteria are the important contributors to the production of organic carbon.
引文
[1]林秋奇,韩博平.水库生态系统特征研究及其在水库水质管理中的应用[J].生态学报,2001,21(6):1034-1040.Lin Q Q,Han B P. Reservoir limnology and its application in water quality management:an overview[J]. Acta Ecologica Sinica,2001,21(6):1034-1040.
[2] Cho B C,Azam F. Major role of bacteria in biogeochemical fluxes in the ocean's interior[J]. Nature,1988,332(6163):441-443.
[3] Woo S H,Enders A,Lehmann J. Microbial mineralization of pyrogenic organic matter in different mineral systems[J].Organic Geochemistry,2016,98:18-26.
[4] Azam F,Malfatti F. Microbial structuring of marine ecosystems[J]. Nature Reviews Microbiology,2007,5(10):782-791.
[5] Zou K J,Thébault E,Lacroix G,et al. Interactions between the green and brown food web determine ecosystem functioning[J].Functional Ecology,2016,30(8):1454-1465.
[6] Matulich K L,Martiny J B H. Microbial composition alters the response of litter decomposition to environmental change[J].Ecology,2016,96(1):154-163.
[7]赛·巴雅尔图,黄瑾,谢贵娟,等.新疆博斯腾湖浮游细菌丰度对富营养化及咸化的响应[J].湖泊科学,2011,23(6):934-941.Sai B,Huang J,Xie G J,et al. Response of planktonic bacterial abundance to eutrophication and salinization in Lake Bosten,Xinjiang[J]. Journal of Lake Sciences,2011,23(6):934-941.
[8]李建洋,赵月,谢宁栋,等.深圳近海表层浮游细菌分布特征及其环境影响因素[J].生态学报,2017,37(24):8567-8577.Li J Y,Zhao Y,Xie N D,et al. Distribution characteristics of bacterioplankton and effect of environmental factors in the coastal surface waters of Shenzhen[J]. Acta Ecologica Sinica,2017,37(24):8567-8577.
[9]周菁,余正,刘开国,等.典型亚热带热分层水库秋季细菌群落垂直分布[J].生态学报,2014,34(21):6205-6213.Zhou J,Yu Z,Liu K G,et al. Bacterial community and its relation to environmental variables in a subtropical stratified reservoir for drinking water supply in autumn[J]. Acta Ecologica Sinica,2014,34(21):6205-6213.
[10]胡明明,王英才,李艳晖,等.云南高原湖库浮游细菌的生态分布及其影响因素[J].水生态学杂志,2011,32(3):21-25.Hu M M,Wang Y C,Li Y H,et al. The ecological distribution of bacterioplankton and its influencing factors in the Yunnan Plateau Lakes[J]. Journal of Hydroecology,2011,32(3):21-25.
[11]广西壮族自治区水利厅.广西壮族自治区水利厅[EB/OL].http://www. gxwater. gov. cn,2018-04-01.
[12]雷恒新,姚星辉.区域地质水文普查报告[R].南宁:广西壮族自治区地质局,1979. 6-90.
[13]李建鸿,蒲俊兵,袁道先,等.岩溶区地下水补给型水库表层无机碳时空变化特征及影响因素[J].环境科学,2015,36(8):2833-2842.Li J H,Pu J B,Yuan D X,et al. Variations of inorganic carbon and its impact factors in surface-layer waters in a groundwater-fed reservoir in karst area,SW China[J]. Environmental Science,2015,36(8):2833-2842.
[14]刘文.亚热带不同地质背景水库碳转移过程的研究[D].重庆:西南大学,2015.
[15]彭文杰,李强,宋昂,等.五里峡水库初级生产力对水气界面二氧化碳和甲烷排放速率时空变化的影响[J].环境科学,2018,39(6):2673-2679.Peng W J,Li Q,Song A,et al. Spatial-temporal variations of CO2and CH4flux through water-air interface under the effect of primary productivity in Wulixia reservoir[J]. Environmental Science,2018,39(6):2673-2679.
[16]卢晓漩,李强,靳振江,等.桂林五里峡水库丰水期溶解有机碳特征[J].环境科学,2018,39(5):2075-2085.Lu X X, Li Q, Jin Z J, et al. Water chemistry and characteristics of dissolved organic carbon in wet season from Wulixia reservoir,SW China[J]. Environmental Science,2018,39(5):2075-2085.
[17] Manerkar M A,Seena S,Brlocher F. Q-RT-PCR for assessing archaea,bacteria, and fungi during leaf decomposition in a stream[J]. Microbial Ecology,2008,56(3):467-473.
[18]邓建才,陈桥,翟水晶,等.太湖水体中氮、磷空间分布特征及环境效应[J].环境科学,2008,29(12):3382-3386.Deng J C, Chen Q, Zhai S J, et al. Spatial distribution characteristics and environmental effect of N and P in water body of Taihu lake[J]. Environmental Science,2008,29(12):3382-3386.
[19]匡武名,张萌,张金美,等.鄱阳湖外围湖泊水体营养波动周年特征的比较湖沼学研究[J].湖泊科学,2016,28(6):1293-1305.Kuang W M, Zhang M, Zhang J M, et al. Comparative limnological study on annual dynamic pattern of nutrients in water column of three suburb lakes adjacent to Lake Poyang[J].Journal of Lake Sciences,2016,28(6):1293-1305.
[20]世界经济合作与发展组织.水体富营养化监测评价与防治[M].北京:中国环境科学出版社,1989. 92-95.
[21]王震,邹华,杨桂军,等.太湖叶绿素a的时空分布特征及其与环境因子的相关关系[J].湖泊科学,2014,26(4):567-575.Wang Z,Ma J,Yang G J,et al. Spatial-temporal characteristics of chlorophyll-a and its relationship with environmental factors in Lake Taihu[J]. Journal of Lake Sciences,2014,26(4):567-575.
[22]王斌,马健,王银亚,等.天山天池夏季叶绿素a的分布及富营养化特征研究[J].环境科学,2015,36(7):2465-2471.Wang B,Ma J,Wang Y Y,et al. Distribution of chlorophyll-a and eutrophication state in Tianchi lake of Tianshan mountains in summer[J]. Environmental Science,2015,36(7):2465-2471.
[23]宋昂,彭文杰,何若雪,等.好氧不产氧光合细菌反馈作用下的五里峡水库坝前水体化学特征研究[J].岩矿测试,2017,36(2):171-179.Song A, Peng W J, He R X, et al. Hydrochemistry characteristics in front of the Wulixia reservoir dam associated with feedback from aerobic anoxygenic phototrophic bacteria[J].Rock and Mineral Analysis,2017,36(2):171-179.
[24]富冰冰.珠江口厌氧氨氧化细菌丰度、群落结构及其环境响应[D].青岛:中国海洋大学,2014.
[25] Song H,Xu J H,Lavoie M,et al. Biological and chemical factors driving the temporal distribution of cyanobacteria and heterotrophic bacteria in a eutrophic lake(West Lake,China)[J]. Applied Microbiology and Biotechnology,2017,101(4):1685-1696.
[26]胡安谊,李姜维,杨晓永,等.宁波三江口水域原核生物群落结构分析[J].环境科学,2015,36(7):2487-2495.Hu A Y,Li J W,Yang X Y,et al. Analysis of prokaryotic community structure in river waters of the Ningbo Sanjiang Mouth[J]. Environmental Science,2015,36(7):2487-2495.
[27] Gao Y, He J F, Chen M, et al. Factors dominating bacterioplankton abundance and production in the Nordic seas and the Chukchi Sea in summer 2012[J]. Acta Oceanologica Sinica,2017,36(8):153-162.
[28]刘子琳,越川海,宁修仁,等.长江冲淡水区细菌生产力研究[J].海洋学报,2001,23(4):93-99.Liu Z L, Yue C H, Ning X R, et al. Bacterioplankton production in dilution zone of the Changjiang(Yangtze River)estuary[J]. Acta Oceanologica Sinica,2001,23(4):93-99.
[29]彭安国,黄奕普,刘广山,等.大亚湾细菌生产力研究[J].海洋学报,2003,25(4):83-90.Peng A G,Huang Y P,Liu G S,et al. Study on bacterial production in the Daya Bay[J]. Acta Oceanologica Sinica,2003,25(4):83-90.
[30] Lee C W. Bacterial abundance and production and heterotrophic nanoflagellate abundance in subarctic coastal waters(Western North Pacific Ocean)[J]. Aquatic Microbial Ecology,2001,23(3):263-271.
[31]季凤云,郭立梅,李洪波,等.南海北部细菌丰度和细菌生产力分布及其与环境因子相关性[J].海洋环境科学,2017,36(3):354-359.Ji F Y,Guo L M,Li H B,et al. Abundance and production of bacteria and their correlations with environmental factor[J].Marine Environmental Science,2017,36(3):354-359.
[32] Yucel N. Seasonal and spatial variation of bacterial production and abundance in the northern Levantine Sea[J]. Mediterranean Marine Science,2017,18(1):97-106.
[33] Puddu A,La Ferla R,Allegra A,et al. Seasonal and spatial distribution of bacterial production and biomass along a salinity gradient(Northern Adriatic Sea)[J]. Hydrobiologia,1997,363(1-3):271-282.
[34] Tuomi P, Suominen K, Autio R. Phytoplankton and bacterioplankton production and bacterial biomass in a fjord-like bay—open sea gradient[J]. Hydrobiologia,1999,393:141-150.
[35]宋星宇,刘华雪,黄良民,等.南海北部夏季基础生物生产力分布特征及影响因素[J].生态学报,2010,30(23):6409-6417.Song X Y, Liu H X, Huang L M, et al. Distribution characteristics of basic biological production and its influencing factors in the northern South China Sea in summer[J]. Acta Ecologica Sinica,2010,30(23):6409-6417.
[36] Jain A,Bandekar M,Gomes J,et al. Temporally invariable bacterial community structure in the Arabian Sea oxygen minimum zone[J]. Aquatic Microbial Ecology,2014,73(1):51-67.
[37] Lu H F,Zhang G M,Wan T,et al. Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation:different metabolic pathways[J]. Bioresource Technology,2011,102(20):9503-9508.
[38]王博雯,汤祥明,高光,等.博斯腾湖细菌丰度时空分布及其与环境因子的关系[J].生态学报,2014,34(7):1812-1821.Wang B W,Tang X M,Gao G,et al. Spatiotemporal dynamics of bacterial abundance and related environmental parameters in Lake Bosten[J]. Acta Ecologica Sinica,2014,34(7):1812-1821.
[39] Haukka K,Kolmonen E,Hyder R,et al. Effect of nutrient loading on bacterioplankton community composition in lake mesocosms[J]. Microbial Ecology,2006,51(2):137-146.
[40] Lewicka-Rataj K,Kaczorkiewicz M,Heese T,et al. Structure and function of bacterioplankton in the rehabilitated Lake Trzesiecko[J]. Ecohydrology&Hydrobiology,2014,14(1):96-105.
[41]陈祯,何聃,任丽娟.温度和营养盐水平对淡水浮游细菌群落结构的潜在影响[J].南京大学学报(自然科学版),2016,52(4):590-600.Chen Z,He D,Ren L J. The potential impacts of temperature and nutrient levels on freshwater bacterioplankton community structure[J]. Journal of Nanjing University(Natural Sciences),2016,52(4):590-600.
[42] Kuosa H,Kaartokallio H. Experimental evidence on nutrient and substrate limitation of Baltic Sea sea-ice algae and bacteria[J].Hydrobiologia,2006,554(1):1-10.
[43]张喆,孟祥红,肖慧,等.青岛近岸水体夏冬季浮游病毒、细菌分布特征及其与环境因子的关系[J].武汉大学学报(理学版),2008,54(2):209-214.Zhang Z,Meng X H,Xiao H,et al. Distribution characteristics of viruses and bacteria and their relationships with environmental factors in summer and winter in coastal waters of Qingdao[J].Journal of Wuhan University(Natural Science Edition),2008,54(2):209-214.
[44] Pomeroy L R, Wiebe W J. Temperature and substrates as interactive limiting factors for marine heterotrophic bacteria[J].Aquatic Microbial Ecology,2001,23(2):187-204.
[45] Niu Y, Shen H,Chen J,et al. Phytoplankton community succession shaping bacterioplankton community composition in Lake Taihu,China[J]. Water Research,2011,45(14):4169-4182.
[46]沈烽,赵大勇,黄睿,等.南京玄武湖浮游细菌群落结构的季节变化及其与环境因子的关系[J].湖泊科学,2017,29(3):662-669.Shen F,Zhao D Y,Huang R,et al. Seasonal variation of bacterioplankton community structure in Xuanwu Lake(Nanjing)and its relationship with environmental factors[J]. Journal of Lake Sciences,2017,29(3):662-669.
[47] Nikrad M P,Cottrell M T,Kirchman D L. Abundance and single-cell activity of heterotrophic bacterial groups in the western Arctic Ocean in summer and winter[J]. Applied and Environmental Microbiology,2012,78(7):2402-2409.
[48] Liu X J,Xu X Q,Xie P,et al. Seasonal changes of dissolved and particulate organic carbon in Donghu Lake, China[J].Chinese Journal of Oceanology and Limnology,2000,18(4):372-377.
[49]王秀君,房传苓,于志同,等.新疆博斯腾湖水体颗粒和溶解有机碳的季节变化及其来源初探[J].湖泊科学,2014,26(4):552-558.Wang X J,Fang C L,Yu Z T,et al. Seasonal variations and sources of particulate and dissolved organic carbon in Lake Bosten,Xinjiang Province[J]. Journal of Lake Sciences,2014,26(4):552-558.
[50] Boteva S,Traykov I,Kenarova A,et al. Abundance and spatial dynamics of bacterioplankton in the Seven Rila Lakes,Bulgaria[J]. Chinese Journal of Oceanology and Limnology,2010,28(3):451-458.
[51] Bricker S B,Ferreira J G,Simas T. An integrated methodology for assessment of estuarine trophic status[J]. Ecological Modelling,2003,169(1):39-60.
[52]倪健斌,张朝霞,柯才焕,等.北部湾夏、冬两季异养细菌的水平分布特征及其影响因子[J].厦门大学学报(自然科学版),2012,51(3):434-440.Ni J B,Zhang Z X,Ke C H,et al. Horizontal distribution characteristics and influencing factors of heterotrophic bacterial abundance in Beibu Gulf in summer and winter[J]. Journal of Xiamen University(Natural Science),2012,51(3):434-440.
[53] Szelag-Wasielewska E,Joniak T,Micha? kiewicz M,et al.Bacterioplankton of the Warta River in relation to physicochemical parameters and flow rate[J]. Ecohydrology&Hydrobiology,2009,9(2-4):225-236.
[54] Li Q, Song A, Peng W J, et al. Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments,Southwest China:Evidence from hydrochemical and microbiological study[J].FEMS Microbiology Ecology,2017,93(6),DOI:10. 1093/femsec/fix065.
[55]姜发军,胡章立,胡超群.大鹏湾浮游细菌时空分布与环境因子的关系[J].热带海洋学报,2011,30(1):96-100.Jiang F J, Hu Z L, Hu C Q. Correlation between spatialtemporal distribution of bacterioplankton and environmental factors in the Dapeng Bay[J]. Journal of Tropical Oceanography,2011,30(1):96-100.
[56]白洁,李岿然,李正炎,等.渤海春季浮游细菌分布与生态环境因子的关系[J].青岛海洋大学学报,2003,33(6):841-846.Bai J, Li K R, Li Z Y, et al. Relationship between the environmental factors and distribution of bacterioplankton in the Bohai sea[J]. Journal of Ocean University of Qingdao,2003,33(6):841-846.
[57]吴娅,王雨春,胡明明,等.三峡库区典型支流浮游细菌的生态分布及其影响因素[J].生态学杂志,2015,34(4):1060-1065.Wu Y,Wang Y C,Hu M M,et al. Ecological distribution and its influencing factors of bacterioplankton in the typical tributaries of Three Gorges Reservoir[J]. Chinese Journal of Ecology,2015,34(4):1060-1065.
[58] Pakulski J D,Baldwin A,Dean A L,et al. Responses of heterotrophic bacteria to solar irradiance in the eastern Pacific Ocean[J]. Aquatic Microbial Ecology,2007,47(2):153-162.
[59]李建鸿,蒲俊兵,孙平安,等.不同地质背景水库区夏季水-气界面温室气体交换通量研究[J].环境科学,2015,36(11):4032-4042.Li J H,Pu J B,Sun P A,et al. Summer greenhouse gases exchange flux across water-air interface in three water reservoirs located in different geologic setting in Guangxi, China[J].Environmental Science,2015,36(11):4032-4042.
[60] Liu Z H,Zhao M,Sun H L,et al.“Old”carbon entering the South China Sea from the carbonate-rich Pearl River Basin:coupled action of carbonate weathering and aquatic photosynthesis[J]. Applied Geochemistry,2017,78:96-104.
[2] Cho B C,Azam F. Major role of bacteria in biogeochemical fluxes in the ocean's interior[J]. Nature,1988,332(6163):441-443.
[3] Woo S H,Enders A,Lehmann J. Microbial mineralization of pyrogenic organic matter in different mineral systems[J].Organic Geochemistry,2016,98:18-26.
[4] Azam F,Malfatti F. Microbial structuring of marine ecosystems[J]. Nature Reviews Microbiology,2007,5(10):782-791.
[5] Zou K J,Thébault E,Lacroix G,et al. Interactions between the green and brown food web determine ecosystem functioning[J].Functional Ecology,2016,30(8):1454-1465.
[6] Matulich K L,Martiny J B H. Microbial composition alters the response of litter decomposition to environmental change[J].Ecology,2016,96(1):154-163.
[7]赛·巴雅尔图,黄瑾,谢贵娟,等.新疆博斯腾湖浮游细菌丰度对富营养化及咸化的响应[J].湖泊科学,2011,23(6):934-941.Sai B,Huang J,Xie G J,et al. Response of planktonic bacterial abundance to eutrophication and salinization in Lake Bosten,Xinjiang[J]. Journal of Lake Sciences,2011,23(6):934-941.
[8]李建洋,赵月,谢宁栋,等.深圳近海表层浮游细菌分布特征及其环境影响因素[J].生态学报,2017,37(24):8567-8577.Li J Y,Zhao Y,Xie N D,et al. Distribution characteristics of bacterioplankton and effect of environmental factors in the coastal surface waters of Shenzhen[J]. Acta Ecologica Sinica,2017,37(24):8567-8577.
[9]周菁,余正,刘开国,等.典型亚热带热分层水库秋季细菌群落垂直分布[J].生态学报,2014,34(21):6205-6213.Zhou J,Yu Z,Liu K G,et al. Bacterial community and its relation to environmental variables in a subtropical stratified reservoir for drinking water supply in autumn[J]. Acta Ecologica Sinica,2014,34(21):6205-6213.
[10]胡明明,王英才,李艳晖,等.云南高原湖库浮游细菌的生态分布及其影响因素[J].水生态学杂志,2011,32(3):21-25.Hu M M,Wang Y C,Li Y H,et al. The ecological distribution of bacterioplankton and its influencing factors in the Yunnan Plateau Lakes[J]. Journal of Hydroecology,2011,32(3):21-25.
[11]广西壮族自治区水利厅.广西壮族自治区水利厅[EB/OL].http://www. gxwater. gov. cn,2018-04-01.
[12]雷恒新,姚星辉.区域地质水文普查报告[R].南宁:广西壮族自治区地质局,1979. 6-90.
[13]李建鸿,蒲俊兵,袁道先,等.岩溶区地下水补给型水库表层无机碳时空变化特征及影响因素[J].环境科学,2015,36(8):2833-2842.Li J H,Pu J B,Yuan D X,et al. Variations of inorganic carbon and its impact factors in surface-layer waters in a groundwater-fed reservoir in karst area,SW China[J]. Environmental Science,2015,36(8):2833-2842.
[14]刘文.亚热带不同地质背景水库碳转移过程的研究[D].重庆:西南大学,2015.
[15]彭文杰,李强,宋昂,等.五里峡水库初级生产力对水气界面二氧化碳和甲烷排放速率时空变化的影响[J].环境科学,2018,39(6):2673-2679.Peng W J,Li Q,Song A,et al. Spatial-temporal variations of CO2and CH4flux through water-air interface under the effect of primary productivity in Wulixia reservoir[J]. Environmental Science,2018,39(6):2673-2679.
[16]卢晓漩,李强,靳振江,等.桂林五里峡水库丰水期溶解有机碳特征[J].环境科学,2018,39(5):2075-2085.Lu X X, Li Q, Jin Z J, et al. Water chemistry and characteristics of dissolved organic carbon in wet season from Wulixia reservoir,SW China[J]. Environmental Science,2018,39(5):2075-2085.
[17] Manerkar M A,Seena S,Brlocher F. Q-RT-PCR for assessing archaea,bacteria, and fungi during leaf decomposition in a stream[J]. Microbial Ecology,2008,56(3):467-473.
[18]邓建才,陈桥,翟水晶,等.太湖水体中氮、磷空间分布特征及环境效应[J].环境科学,2008,29(12):3382-3386.Deng J C, Chen Q, Zhai S J, et al. Spatial distribution characteristics and environmental effect of N and P in water body of Taihu lake[J]. Environmental Science,2008,29(12):3382-3386.
[19]匡武名,张萌,张金美,等.鄱阳湖外围湖泊水体营养波动周年特征的比较湖沼学研究[J].湖泊科学,2016,28(6):1293-1305.Kuang W M, Zhang M, Zhang J M, et al. Comparative limnological study on annual dynamic pattern of nutrients in water column of three suburb lakes adjacent to Lake Poyang[J].Journal of Lake Sciences,2016,28(6):1293-1305.
[20]世界经济合作与发展组织.水体富营养化监测评价与防治[M].北京:中国环境科学出版社,1989. 92-95.
[21]王震,邹华,杨桂军,等.太湖叶绿素a的时空分布特征及其与环境因子的相关关系[J].湖泊科学,2014,26(4):567-575.Wang Z,Ma J,Yang G J,et al. Spatial-temporal characteristics of chlorophyll-a and its relationship with environmental factors in Lake Taihu[J]. Journal of Lake Sciences,2014,26(4):567-575.
[22]王斌,马健,王银亚,等.天山天池夏季叶绿素a的分布及富营养化特征研究[J].环境科学,2015,36(7):2465-2471.Wang B,Ma J,Wang Y Y,et al. Distribution of chlorophyll-a and eutrophication state in Tianchi lake of Tianshan mountains in summer[J]. Environmental Science,2015,36(7):2465-2471.
[23]宋昂,彭文杰,何若雪,等.好氧不产氧光合细菌反馈作用下的五里峡水库坝前水体化学特征研究[J].岩矿测试,2017,36(2):171-179.Song A, Peng W J, He R X, et al. Hydrochemistry characteristics in front of the Wulixia reservoir dam associated with feedback from aerobic anoxygenic phototrophic bacteria[J].Rock and Mineral Analysis,2017,36(2):171-179.
[24]富冰冰.珠江口厌氧氨氧化细菌丰度、群落结构及其环境响应[D].青岛:中国海洋大学,2014.
[25] Song H,Xu J H,Lavoie M,et al. Biological and chemical factors driving the temporal distribution of cyanobacteria and heterotrophic bacteria in a eutrophic lake(West Lake,China)[J]. Applied Microbiology and Biotechnology,2017,101(4):1685-1696.
[26]胡安谊,李姜维,杨晓永,等.宁波三江口水域原核生物群落结构分析[J].环境科学,2015,36(7):2487-2495.Hu A Y,Li J W,Yang X Y,et al. Analysis of prokaryotic community structure in river waters of the Ningbo Sanjiang Mouth[J]. Environmental Science,2015,36(7):2487-2495.
[27] Gao Y, He J F, Chen M, et al. Factors dominating bacterioplankton abundance and production in the Nordic seas and the Chukchi Sea in summer 2012[J]. Acta Oceanologica Sinica,2017,36(8):153-162.
[28]刘子琳,越川海,宁修仁,等.长江冲淡水区细菌生产力研究[J].海洋学报,2001,23(4):93-99.Liu Z L, Yue C H, Ning X R, et al. Bacterioplankton production in dilution zone of the Changjiang(Yangtze River)estuary[J]. Acta Oceanologica Sinica,2001,23(4):93-99.
[29]彭安国,黄奕普,刘广山,等.大亚湾细菌生产力研究[J].海洋学报,2003,25(4):83-90.Peng A G,Huang Y P,Liu G S,et al. Study on bacterial production in the Daya Bay[J]. Acta Oceanologica Sinica,2003,25(4):83-90.
[30] Lee C W. Bacterial abundance and production and heterotrophic nanoflagellate abundance in subarctic coastal waters(Western North Pacific Ocean)[J]. Aquatic Microbial Ecology,2001,23(3):263-271.
[31]季凤云,郭立梅,李洪波,等.南海北部细菌丰度和细菌生产力分布及其与环境因子相关性[J].海洋环境科学,2017,36(3):354-359.Ji F Y,Guo L M,Li H B,et al. Abundance and production of bacteria and their correlations with environmental factor[J].Marine Environmental Science,2017,36(3):354-359.
[32] Yucel N. Seasonal and spatial variation of bacterial production and abundance in the northern Levantine Sea[J]. Mediterranean Marine Science,2017,18(1):97-106.
[33] Puddu A,La Ferla R,Allegra A,et al. Seasonal and spatial distribution of bacterial production and biomass along a salinity gradient(Northern Adriatic Sea)[J]. Hydrobiologia,1997,363(1-3):271-282.
[34] Tuomi P, Suominen K, Autio R. Phytoplankton and bacterioplankton production and bacterial biomass in a fjord-like bay—open sea gradient[J]. Hydrobiologia,1999,393:141-150.
[35]宋星宇,刘华雪,黄良民,等.南海北部夏季基础生物生产力分布特征及影响因素[J].生态学报,2010,30(23):6409-6417.Song X Y, Liu H X, Huang L M, et al. Distribution characteristics of basic biological production and its influencing factors in the northern South China Sea in summer[J]. Acta Ecologica Sinica,2010,30(23):6409-6417.
[36] Jain A,Bandekar M,Gomes J,et al. Temporally invariable bacterial community structure in the Arabian Sea oxygen minimum zone[J]. Aquatic Microbial Ecology,2014,73(1):51-67.
[37] Lu H F,Zhang G M,Wan T,et al. Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation:different metabolic pathways[J]. Bioresource Technology,2011,102(20):9503-9508.
[38]王博雯,汤祥明,高光,等.博斯腾湖细菌丰度时空分布及其与环境因子的关系[J].生态学报,2014,34(7):1812-1821.Wang B W,Tang X M,Gao G,et al. Spatiotemporal dynamics of bacterial abundance and related environmental parameters in Lake Bosten[J]. Acta Ecologica Sinica,2014,34(7):1812-1821.
[39] Haukka K,Kolmonen E,Hyder R,et al. Effect of nutrient loading on bacterioplankton community composition in lake mesocosms[J]. Microbial Ecology,2006,51(2):137-146.
[40] Lewicka-Rataj K,Kaczorkiewicz M,Heese T,et al. Structure and function of bacterioplankton in the rehabilitated Lake Trzesiecko[J]. Ecohydrology&Hydrobiology,2014,14(1):96-105.
[41]陈祯,何聃,任丽娟.温度和营养盐水平对淡水浮游细菌群落结构的潜在影响[J].南京大学学报(自然科学版),2016,52(4):590-600.Chen Z,He D,Ren L J. The potential impacts of temperature and nutrient levels on freshwater bacterioplankton community structure[J]. Journal of Nanjing University(Natural Sciences),2016,52(4):590-600.
[42] Kuosa H,Kaartokallio H. Experimental evidence on nutrient and substrate limitation of Baltic Sea sea-ice algae and bacteria[J].Hydrobiologia,2006,554(1):1-10.
[43]张喆,孟祥红,肖慧,等.青岛近岸水体夏冬季浮游病毒、细菌分布特征及其与环境因子的关系[J].武汉大学学报(理学版),2008,54(2):209-214.Zhang Z,Meng X H,Xiao H,et al. Distribution characteristics of viruses and bacteria and their relationships with environmental factors in summer and winter in coastal waters of Qingdao[J].Journal of Wuhan University(Natural Science Edition),2008,54(2):209-214.
[44] Pomeroy L R, Wiebe W J. Temperature and substrates as interactive limiting factors for marine heterotrophic bacteria[J].Aquatic Microbial Ecology,2001,23(2):187-204.
[45] Niu Y, Shen H,Chen J,et al. Phytoplankton community succession shaping bacterioplankton community composition in Lake Taihu,China[J]. Water Research,2011,45(14):4169-4182.
[46]沈烽,赵大勇,黄睿,等.南京玄武湖浮游细菌群落结构的季节变化及其与环境因子的关系[J].湖泊科学,2017,29(3):662-669.Shen F,Zhao D Y,Huang R,et al. Seasonal variation of bacterioplankton community structure in Xuanwu Lake(Nanjing)and its relationship with environmental factors[J]. Journal of Lake Sciences,2017,29(3):662-669.
[47] Nikrad M P,Cottrell M T,Kirchman D L. Abundance and single-cell activity of heterotrophic bacterial groups in the western Arctic Ocean in summer and winter[J]. Applied and Environmental Microbiology,2012,78(7):2402-2409.
[48] Liu X J,Xu X Q,Xie P,et al. Seasonal changes of dissolved and particulate organic carbon in Donghu Lake, China[J].Chinese Journal of Oceanology and Limnology,2000,18(4):372-377.
[49]王秀君,房传苓,于志同,等.新疆博斯腾湖水体颗粒和溶解有机碳的季节变化及其来源初探[J].湖泊科学,2014,26(4):552-558.Wang X J,Fang C L,Yu Z T,et al. Seasonal variations and sources of particulate and dissolved organic carbon in Lake Bosten,Xinjiang Province[J]. Journal of Lake Sciences,2014,26(4):552-558.
[50] Boteva S,Traykov I,Kenarova A,et al. Abundance and spatial dynamics of bacterioplankton in the Seven Rila Lakes,Bulgaria[J]. Chinese Journal of Oceanology and Limnology,2010,28(3):451-458.
[51] Bricker S B,Ferreira J G,Simas T. An integrated methodology for assessment of estuarine trophic status[J]. Ecological Modelling,2003,169(1):39-60.
[52]倪健斌,张朝霞,柯才焕,等.北部湾夏、冬两季异养细菌的水平分布特征及其影响因子[J].厦门大学学报(自然科学版),2012,51(3):434-440.Ni J B,Zhang Z X,Ke C H,et al. Horizontal distribution characteristics and influencing factors of heterotrophic bacterial abundance in Beibu Gulf in summer and winter[J]. Journal of Xiamen University(Natural Science),2012,51(3):434-440.
[53] Szelag-Wasielewska E,Joniak T,Micha? kiewicz M,et al.Bacterioplankton of the Warta River in relation to physicochemical parameters and flow rate[J]. Ecohydrology&Hydrobiology,2009,9(2-4):225-236.
[54] Li Q, Song A, Peng W J, et al. Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments,Southwest China:Evidence from hydrochemical and microbiological study[J].FEMS Microbiology Ecology,2017,93(6),DOI:10. 1093/femsec/fix065.
[55]姜发军,胡章立,胡超群.大鹏湾浮游细菌时空分布与环境因子的关系[J].热带海洋学报,2011,30(1):96-100.Jiang F J, Hu Z L, Hu C Q. Correlation between spatialtemporal distribution of bacterioplankton and environmental factors in the Dapeng Bay[J]. Journal of Tropical Oceanography,2011,30(1):96-100.
[56]白洁,李岿然,李正炎,等.渤海春季浮游细菌分布与生态环境因子的关系[J].青岛海洋大学学报,2003,33(6):841-846.Bai J, Li K R, Li Z Y, et al. Relationship between the environmental factors and distribution of bacterioplankton in the Bohai sea[J]. Journal of Ocean University of Qingdao,2003,33(6):841-846.
[57]吴娅,王雨春,胡明明,等.三峡库区典型支流浮游细菌的生态分布及其影响因素[J].生态学杂志,2015,34(4):1060-1065.Wu Y,Wang Y C,Hu M M,et al. Ecological distribution and its influencing factors of bacterioplankton in the typical tributaries of Three Gorges Reservoir[J]. Chinese Journal of Ecology,2015,34(4):1060-1065.
[58] Pakulski J D,Baldwin A,Dean A L,et al. Responses of heterotrophic bacteria to solar irradiance in the eastern Pacific Ocean[J]. Aquatic Microbial Ecology,2007,47(2):153-162.
[59]李建鸿,蒲俊兵,孙平安,等.不同地质背景水库区夏季水-气界面温室气体交换通量研究[J].环境科学,2015,36(11):4032-4042.Li J H,Pu J B,Sun P A,et al. Summer greenhouse gases exchange flux across water-air interface in three water reservoirs located in different geologic setting in Guangxi, China[J].Environmental Science,2015,36(11):4032-4042.
[60] Liu Z H,Zhao M,Sun H L,et al.“Old”carbon entering the South China Sea from the carbonate-rich Pearl River Basin:coupled action of carbonate weathering and aquatic photosynthesis[J]. Applied Geochemistry,2017,78:96-104.