RTCB在小鼠不同组织中的表达
|
摘要
目的检测RTCB在小鼠各种组织中的表达和分布。方法提取小鼠胃、前列腺、子宫、卵巢等组织的总蛋白,利用免疫印迹技术,检测RTCB在各组织的相对表达量;通过免疫组织化学技术,检测RTCB在各组织的表达定位情况。结果免疫印迹结果显示,小鼠17种组织中RTCB均有表达,输卵管、骨骼肌、肾脏的相对表达量较高,而在膀胱和小肠的相对表达量较低。免疫组织化学结果显示,RTCB在不同组织或同一组织的不同细胞中免疫染色存在差异,尤其在睾丸、附睾、卵巢、子宫、输卵管、小肠等组织某些特定细胞中免疫染色较强。结论 RTCB可能参与小鼠生殖系统功能的维持。
Objective To investigate the expression and distribution of RTCB in various tissues of mice. Methods We detected the expression of RTCB by Western blotting and the location of the expression of RTCB in each tissue by immunohistochemistry. Results RTCB protein was all expressed in 17 tissues of mice. The relative expressions of oviduct, skeletal muscle and kidney were high, while the relative expressions of bladder and small intestine were low. In addition, the immunostaining of RTCB was different in different tissues or in different cells of the same tissue. The immunostaining was stronger in some specific cells of testis, epididymis, ovary, uterus, fallopian tube and small intestine. Conclusion RTCB may participate in the maintenance of reproductive function in mice.
Objective To investigate the expression and distribution of RTCB in various tissues of mice. Methods We detected the expression of RTCB by Western blotting and the location of the expression of RTCB in each tissue by immunohistochemistry. Results RTCB protein was all expressed in 17 tissues of mice. The relative expressions of oviduct, skeletal muscle and kidney were high, while the relative expressions of bladder and small intestine were low. In addition, the immunostaining of RTCB was different in different tissues or in different cells of the same tissue. The immunostaining was stronger in some specific cells of testis, epididymis, ovary, uterus, fallopian tube and small intestine. Conclusion RTCB may participate in the maintenance of reproductive function in mice.
引文
[1] Tanaka N,Chakravarty AK,Maughan B,et al.Novel mechanism of RNA repair by RtcB via sequential 2’,3’-cyclic phosphodiesterase and 3’-Phosphate/5’-hydroxyl ligation reactions[J].J Biol Chem,2011,286(50):43134-43143.
[2] Tanaka N,Meineke B,Shuman S.RtcB,a novel RNA ligase,can catalyze tRNA splicing and HAC1 mRNA splicing in vivo[J].J Biol Chem,2011,286(35):30253-30257.
[3] Zhang X,Ma H,Liu D,et al.Expression analysis of mouse focal adhesion associated protein[J].Animal Husbandry Veterinary,2013,45(6):59-61.(in Chinese)张旭,马红,刘娣,等.小鼠黏着斑相关蛋白在不同组织中的表达分析[J].畜牧与兽医,2013,45(6):59-61.
[4] Zhao JD,Ju XF,Zhang LQ,et al.Spatiotemporal expression of D10Wsu52e gene in the developing mouse stomach and duodenum[J].Biophysics Reports,2011,27(9):757-764.(in Chinese)赵疆东,鞠晓芳,张立强,等.D10Wsu52e基因在发育中小鼠胃和十二指肠的时空表达[J].生物物理学报,2011,27(9):757-764.
[5] Ding NZ,He M,He CQ,et al.Expression and regulation of FAAP in the mouse epididymis[J].Endocrine,2010,38(2):188-193.
[6] Zhao J,Ju X,Zhang L,et al.Spatiotemporal expression of D10Wsu52e in the developing mouse pancreas[J].Front Biosci (Elite Ed),2011,3:355-463.
[7] Kosmaczewski SG,Edwards TJ,Han SM,et al.The RtcB RNA ligase is an essential component of the metazoan unfolded protein response[J].EMBO Rep,2014,15(12):1278-1285.
[8] Bai XW,Ji W,Ding BW,et al.Endoplasmic reticulum stress and inflammatory bowel disease in intestinal secretion[J].Chinese Journal of Current Advances in General Surgery,2012,15(1):34-38.(in Chinese)白小武,嵇武,丁博文,等.肠道分泌细胞内质网应激与炎性肠病[J].中国现代普通外科进展,2012,15(1):34-38.
[9] Lu Y,Liang FX,Wang X.A synthetic biology approach identifies the mammalian UPR RNA ligase RtcB[J].Mol Cell,2014,55(5):758-770.
[10] Zeng Ch,Du Y,Ding NZh,The research progress of RtcB function[J].Chemistry of life,2017,37(2):147-152.(in Chinese)曾超,杜燕,丁乃峥,RtcB功能的研究进展[J].生命的化学,2017,37(2):147-152.
[2] Tanaka N,Meineke B,Shuman S.RtcB,a novel RNA ligase,can catalyze tRNA splicing and HAC1 mRNA splicing in vivo[J].J Biol Chem,2011,286(35):30253-30257.
[3] Zhang X,Ma H,Liu D,et al.Expression analysis of mouse focal adhesion associated protein[J].Animal Husbandry Veterinary,2013,45(6):59-61.(in Chinese)张旭,马红,刘娣,等.小鼠黏着斑相关蛋白在不同组织中的表达分析[J].畜牧与兽医,2013,45(6):59-61.
[4] Zhao JD,Ju XF,Zhang LQ,et al.Spatiotemporal expression of D10Wsu52e gene in the developing mouse stomach and duodenum[J].Biophysics Reports,2011,27(9):757-764.(in Chinese)赵疆东,鞠晓芳,张立强,等.D10Wsu52e基因在发育中小鼠胃和十二指肠的时空表达[J].生物物理学报,2011,27(9):757-764.
[5] Ding NZ,He M,He CQ,et al.Expression and regulation of FAAP in the mouse epididymis[J].Endocrine,2010,38(2):188-193.
[6] Zhao J,Ju X,Zhang L,et al.Spatiotemporal expression of D10Wsu52e in the developing mouse pancreas[J].Front Biosci (Elite Ed),2011,3:355-463.
[7] Kosmaczewski SG,Edwards TJ,Han SM,et al.The RtcB RNA ligase is an essential component of the metazoan unfolded protein response[J].EMBO Rep,2014,15(12):1278-1285.
[8] Bai XW,Ji W,Ding BW,et al.Endoplasmic reticulum stress and inflammatory bowel disease in intestinal secretion[J].Chinese Journal of Current Advances in General Surgery,2012,15(1):34-38.(in Chinese)白小武,嵇武,丁博文,等.肠道分泌细胞内质网应激与炎性肠病[J].中国现代普通外科进展,2012,15(1):34-38.
[9] Lu Y,Liang FX,Wang X.A synthetic biology approach identifies the mammalian UPR RNA ligase RtcB[J].Mol Cell,2014,55(5):758-770.
[10] Zeng Ch,Du Y,Ding NZh,The research progress of RtcB function[J].Chemistry of life,2017,37(2):147-152.(in Chinese)曾超,杜燕,丁乃峥,RtcB功能的研究进展[J].生命的化学,2017,37(2):147-152.