微乳薄层色谱法对桑叶桑枝中活性成分的鉴别
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摘要
目的建立微乳薄层色谱法鉴别桑叶桑枝中活性成分的方法。方法 85%乙醇为溶剂,超声提取桑叶、桑枝中的活性成分。以聚酰胺薄膜作为固定相,微乳液为流动相,对桑叶桑枝中的活性成分进行定性鉴别,并考察微乳液的含水量、十二烷基硫酸钠(SDS)用量、助表面活性剂的种类及用量、微乳液中甲酸含量对分离效果的影响。结果最佳展开系统为含水量75%的O/W型微乳液,成分和配比为m(SDS)∶m(正丁醇)∶m(正己烷)=2.7∶6.3∶1.0,V(微乳液)∶V(甲酸)=9.0∶1.0。分别鉴定出桑叶中含有绿原酸、芦丁和桑皮苷A3种活性成分,桑枝中含有桑辛素、东莨菪内酯、绿原酸、桑皮苷A4种活性成分。结论本实验快速简单、点样量小、斑点清晰、重复性好,适用于桑叶桑枝中活性成分的鉴别。
Objective To establish a microemulsion thin layer chromatography method for identifying active components in Folium mori and Ramulus mori.Methods The active constituents of Folium mori and Ramulus mori were extracted by ultrasonic method using 85%ethanol as solvent.The active components were identified qualitatively using the polyamide film as stationary phase and the microemulsion as mobile phase.The effect of water content in microemulsion,amount of SDS and n-butanol,type and dosage of cosurfactant,and formic acid content of microemulsion on separation were investigated.Results The optimal development system was an O/W microemulsion with 75% water content,The composition and ratio were m(sodium dodecyl sulfate)∶ m(n-butanol)∶ m(n-hexane)=2.7∶6.3∶1.0,V(microemulsion)∶V(formic acid)=9.0∶1.0respectively.Three components of chlorogenic acid,rutin and mulberroside A in Folium mori and four components of morusin,scopoletin,chlorogenic acid,and mulberroside A in Ramulus mori were identified.Conclusion This experiment is fast,simple,small sample size,clear spots,and good repeatability,which is suitable for identification of active ingredients in Folium mori and Ramulus mori.
Objective To establish a microemulsion thin layer chromatography method for identifying active components in Folium mori and Ramulus mori.Methods The active constituents of Folium mori and Ramulus mori were extracted by ultrasonic method using 85%ethanol as solvent.The active components were identified qualitatively using the polyamide film as stationary phase and the microemulsion as mobile phase.The effect of water content in microemulsion,amount of SDS and n-butanol,type and dosage of cosurfactant,and formic acid content of microemulsion on separation were investigated.Results The optimal development system was an O/W microemulsion with 75% water content,The composition and ratio were m(sodium dodecyl sulfate)∶ m(n-butanol)∶ m(n-hexane)=2.7∶6.3∶1.0,V(microemulsion)∶V(formic acid)=9.0∶1.0respectively.Three components of chlorogenic acid,rutin and mulberroside A in Folium mori and four components of morusin,scopoletin,chlorogenic acid,and mulberroside A in Ramulus mori were identified.Conclusion This experiment is fast,simple,small sample size,clear spots,and good repeatability,which is suitable for identification of active ingredients in Folium mori and Ramulus mori.
引文
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[2]赖玲林,彭小芳,冷恩念,等.中药桑叶药理作用的研究进展[J].安徽医药,2016,20(12):2210-2213.
[3]向伟,喻艳,刘静,等.新疆药桑枝条中抗氧化活性成sanggenonA的分离鉴定[J].林业科学,2016,52(3):30-35.
[4]黄链莎,刘铜华,孙文,等.桑叶黄酮对糖尿病大鼠血糖水平的影响及机制探讨[J].中国实验方剂学杂志,2018,24(16):152-156.
[5]俞燕芳,黄金枝,石旭平,等.我国桑叶食品开发研究进展[J].食品安全质量检测学报,2018,9(7):1572-1576.
[6]刘超,史正琴,向伟,等.桑枝1-脱氧野尻霉素的提取及其含量与α-葡萄糖苷酶抑制活性的相关性分析[J].林业科学,2015,51(2):112-119.
[7]李琳,刘先明,马永雷,等.从桑枝皮醇提物萃取的不同组分及其体外生物活性[J].蚕业科学,2010,36(4):680-686.
[8]杜鸿志,余子川,袁胜涛.中药石韦中黄酮类成分微乳薄层色谱分析研究[J].亚太传统医药,2014,10(4):58-61.
[9]刘连芬,钱关泽.朝天罐总黄酮的提取及微乳液薄层分析[J].江苏农业科学,2010,38(1):282-283.
[10]李法威,付京,李萍.微乳技术在中药分析中的研究进展[J].中国药物经济学,2018,13(6):109-112.
[11]邬浩杰.两种中药黄酮类成分的微乳薄层色谱鉴别[J].浙江中医杂志,2010,45(1):51-53.
[12]夏提古丽·塔西买买提,胡曙晨,热娜·卡斯木.欧矢车菊根不同极性萃取部位体外抗氧化活性及其总黄酮的微乳薄层研究[J].新疆医科大学学报,2016,39(3):277-282.
[13]孙悦,叶建成,梁生旺,等.微乳液在苦参及其中成药薄层色谱分析中的应用研究[J].时珍国医国药,2008,19(8):2018-2020.
[14]张岩,王强,任常顺,等.心脑脉通胶囊的微乳薄层色谱鉴别[J].药学实践杂志,2015,33(6):536-538.
[15]王艳,张俊妹,郭金凤,等.榆叶合叶子黄酮类成分的微乳薄层色谱鉴别[J].新疆医科大学学报,2015,38(1):61-63.