正交优化超声辅助法提取杜仲叶中绿原酸的工艺研究
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摘要
对杜仲叶中的绿原酸采用超声波辅助技术进行提取研究。根据6种因素对提取结果的影响趋势,采用正交试验优化提取工艺。结果表明最佳提取条件:溶剂pH 4.5、超声浸提时间25 min、超声浸提温度40℃、溶剂中乙醇浓度70%,测得提取物的得率为4.06%。
In this work,ultrasound-assisted extraction technology of chlorogenic acid from Eucommia ulmoides leaves was studied.The effects on extraction of the six factors were explored,and orthogonal test was made and optimized extraction process was obtained.The results showed that,the best condition was solvent pH 4.5,ultrasonic extraction time 25 min,extraction temperature 40 ℃,and ethanol concentration in the solvent 70 %.On this condition the yield could be 4.06 %.
In this work,ultrasound-assisted extraction technology of chlorogenic acid from Eucommia ulmoides leaves was studied.The effects on extraction of the six factors were explored,and orthogonal test was made and optimized extraction process was obtained.The results showed that,the best condition was solvent pH 4.5,ultrasonic extraction time 25 min,extraction temperature 40 ℃,and ethanol concentration in the solvent 70 %.On this condition the yield could be 4.06 %.
引文
[1]李旭,刘停,陈时建,等.杜仲叶绿原酸提取工艺优化及对自发性高血压大鼠的降压作用[J].食品科学,2013,34(14):30-34
[2]王辉,田呈瑞,马守磊,等.绿原酸的研究进展[J].食品工业科技,2009,30(5):341-345
[3]岳鹍,王芃,崔荣健.超声波辅助提取杜仲叶中绿原酸的工艺研究[J].农产品加工(学刊),2010(2):33-35
[4]曾桥,韦承伯,吕生华.杜仲叶主要活性成分研究进展[J].安徽农业科学,2017,45(34):133-135
[5]张蕾,张娜,展雯琳.正交法优选杜仲叶中绿原酸提取工艺[J].现代食品,2016(14):97-100
[6]谢敏.杜仲叶绿原酸提取物对胰脂肪酶活性抑制的研究[J].广州化工,2015,43(6):121-122,134
[7]CHO A S,JEON S M,KIM M J,et al.Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat dietinduced-obese mice[J].Food&Chemical Toxicology,2010,48(3):937-943
[8]赖玲林,肖苑,彭小芳,等.杜仲叶绿原酸提取物联合西红花苷对肝癌细胞胆固醇代谢的影响[J].山东医药,2017,57(8):17-20
[9]胡居吾,韩晓丹,付建平,等.三种绿原酸提取物的抑菌和抗氧化效果比较[J].天然产物研究与开发,2017,29(11):1928-1933
[10]何念武,杨超.杜仲叶中绿原酸的提取及其在果蔬保鲜中的应用[J].江西农业学报,2015,27(7):107-110
[11]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301
[12]肖希斌,陈勇,李颖,等.绿原酸超声波提取工艺研究概况[J].现代农业科技,2018(17):238,241
[13]刘军海,裘爱泳.杜仲叶中绿原酸提取工艺研究[J].食品科学,2006,27(12):450-454
[14]刘军海,裘爱泳.绿原酸的提取分离及含量测定[J].中国油脂,2005,30(3):54-56
[15]谷楠楠,王天贵,肖清贵,等.杜仲叶中绿原酸的浸出动力学[J].过程工程学报,2016,16(4):590-595
[16]兰小艳,黄敏,张学俊.杜仲叶中绿原酸醇提法的工艺研究[J].中国农学通报,2009,25(18):84-88
[2]王辉,田呈瑞,马守磊,等.绿原酸的研究进展[J].食品工业科技,2009,30(5):341-345
[3]岳鹍,王芃,崔荣健.超声波辅助提取杜仲叶中绿原酸的工艺研究[J].农产品加工(学刊),2010(2):33-35
[4]曾桥,韦承伯,吕生华.杜仲叶主要活性成分研究进展[J].安徽农业科学,2017,45(34):133-135
[5]张蕾,张娜,展雯琳.正交法优选杜仲叶中绿原酸提取工艺[J].现代食品,2016(14):97-100
[6]谢敏.杜仲叶绿原酸提取物对胰脂肪酶活性抑制的研究[J].广州化工,2015,43(6):121-122,134
[7]CHO A S,JEON S M,KIM M J,et al.Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat dietinduced-obese mice[J].Food&Chemical Toxicology,2010,48(3):937-943
[8]赖玲林,肖苑,彭小芳,等.杜仲叶绿原酸提取物联合西红花苷对肝癌细胞胆固醇代谢的影响[J].山东医药,2017,57(8):17-20
[9]胡居吾,韩晓丹,付建平,等.三种绿原酸提取物的抑菌和抗氧化效果比较[J].天然产物研究与开发,2017,29(11):1928-1933
[10]何念武,杨超.杜仲叶中绿原酸的提取及其在果蔬保鲜中的应用[J].江西农业学报,2015,27(7):107-110
[11]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301
[12]肖希斌,陈勇,李颖,等.绿原酸超声波提取工艺研究概况[J].现代农业科技,2018(17):238,241
[13]刘军海,裘爱泳.杜仲叶中绿原酸提取工艺研究[J].食品科学,2006,27(12):450-454
[14]刘军海,裘爱泳.绿原酸的提取分离及含量测定[J].中国油脂,2005,30(3):54-56
[15]谷楠楠,王天贵,肖清贵,等.杜仲叶中绿原酸的浸出动力学[J].过程工程学报,2016,16(4):590-595
[16]兰小艳,黄敏,张学俊.杜仲叶中绿原酸醇提法的工艺研究[J].中国农学通报,2009,25(18):84-88