专利方四妙君逸软膏促愈机制的实验研究
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摘要
[目的]研究专利方四妙君逸软膏促愈的作用机制。[方法]将90只大鼠,随机均分为模型组、空白对照组、重组人表皮生长因子组、龙珠软膏组和专利方四妙君逸软膏组5组。建立大鼠皮肤创伤模型后,从宏观整体水平观察不同时相点各组大鼠创面愈合率、组织病理学以及最终创面愈合时间;从微观分子水平观察不同时相点大鼠创面肉芽组织中表皮生长因子受体(EGFR)含量、血清中转化生长因子-α(TGF-α)、白介素-1β(IL-1β)含量。[结果]1)专利方四妙君逸软膏能缩短创面愈合时间(P<0.01),提高创面愈合率。2)专利方四妙君逸软膏能提高大鼠创面EGFR(P<0.01)和血清中TGF-α表达(P<0.01),降低血清中IL-1β的表达(P<0.01),且在第7天时作用最显著。[结论]1)专利方四妙君逸软膏具有良好的促愈作用,且作用与龙珠软膏相当。2)专利方四妙君逸软膏的促愈机制可能与TGF-α、IL-1以及EGFR表达的调节有关。
[Objective] To study the mechanism of promoting wound healing by patented product of Simiao Junyi ointment. [Methods]The 90 rats were randomly divided into model group, blank control group, recombinant human epidermal growth factor group, the Longzhu ointment group and patented product of Simiao Junyi ointment group. Skin wound healing model of rats were successfully established. To observe each group of rats' wound healing time and wound healing rate, histopathology, epidermal growth factor receptor(EGFR), transforming growth factor-alpha(TGF-α), interleukin-1(IL-1) content. [Results] 1) Patented product of Simiao Junyi ointment can shorten wound healing time(P<0.01) and improve the healing rate. 2) The content of the EGFR in granulation tissue(P<0.01) and TGF-α content in serum(P <0.01) was enhanced. The content of serum IL-1β was reduced(P <0.01). [Conclusion] 1) The patented product of Simiao Junyi ointment has an act of promoting wound healing as the same act of Longzhu ointment. 2) The mechanism of promoting wound healing of patented product of Junyi Simiao ointment may related to the regulation of TGF-α, IL-1 and EGFR.
[Objective] To study the mechanism of promoting wound healing by patented product of Simiao Junyi ointment. [Methods]The 90 rats were randomly divided into model group, blank control group, recombinant human epidermal growth factor group, the Longzhu ointment group and patented product of Simiao Junyi ointment group. Skin wound healing model of rats were successfully established. To observe each group of rats' wound healing time and wound healing rate, histopathology, epidermal growth factor receptor(EGFR), transforming growth factor-alpha(TGF-α), interleukin-1(IL-1) content. [Results] 1) Patented product of Simiao Junyi ointment can shorten wound healing time(P<0.01) and improve the healing rate. 2) The content of the EGFR in granulation tissue(P<0.01) and TGF-α content in serum(P <0.01) was enhanced. The content of serum IL-1β was reduced(P <0.01). [Conclusion] 1) The patented product of Simiao Junyi ointment has an act of promoting wound healing as the same act of Longzhu ointment. 2) The mechanism of promoting wound healing of patented product of Junyi Simiao ointment may related to the regulation of TGF-α, IL-1 and EGFR.
引文
[1]何永恒,胡响当.用于肛肠病术后镇痛的四妙君逸软膏组方研究[J].中华中医药杂志,2010,25(10):1694-1697.
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[5]何永恒,张湘杰,罗敏.四妙君逸软膏用于肛肠病术后换药镇痛的药理学实验研究[J].中华中医药杂志,2010,25(11):1871-1874.
[6]何永恒,鲁龙生,王晓艳.四妙君逸软膏用于肛肠病术后止痛促愈的临床研究[J].湖南中医药大学学报,2010,30(11):51-54.
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[12]隋志甫,刘静杰,谷延敏,等.创面修复中骨桥蛋白和表皮生长因子受体表达变化的实验研究[J].中国医药导报,2011,8(17):22-24.
[13]Behm B,Babilas P,Landthaler M,et al.Cytokines,chemokines and growth factors in wound healing[J].Journal of the European Academy of Dermatology and Venereology,2012,26(7):812-820.
[14]王大鹏,张桂梅,罗力佳.细胞生长因子修复骨骼肌损伤[J].中国组织工程研究,2016,20(2):273-278.
[15]吴伟.重组人表皮生长因子与碱性成纤维生长因子促进角膜上皮损伤修复及血管新生[J].广州:南方医科大学,2012.
[16]Dinh T,Braunagel S,Rosenblum BI.Growth Factors in Wound Healing:The Present and the Future?[J].Clinics in podiatric medicine and surgery,2015,32(1):109-119.
[17]The molecular and cellular biology of wound repair[M].Berlin:Springer Science&Business Media,2013.
[18]Eldad A,Weinberg A,Breiterman S,et al.Early nonsurgical removal of chemically injured tissue enhances wound healing in partial thickness burns[J].Burns,1998,24(2):166-172.
[2]金艺淑,何群,何永恒,等.正交试验优选四妙君逸软膏提取工艺[J].中国药房,2011,22(7):603-605.
[3]何群,韩军涛,陈光宇,等.四妙君逸软膏与散剂离体透皮特性的比较研究[J].中成药,2012,34(6):1052-1056.
[4]彭思源,何群,陈光宇,等.四妙君逸软膏制剂处方筛选研究[J].中成药,2015,37(11):2544-2546.
[5]何永恒,张湘杰,罗敏.四妙君逸软膏用于肛肠病术后换药镇痛的药理学实验研究[J].中华中医药杂志,2010,25(11):1871-1874.
[6]何永恒,鲁龙生,王晓艳.四妙君逸软膏用于肛肠病术后止痛促愈的临床研究[J].湖南中医药大学学报,2010,30(11):51-54.
[7]何永恒,罗敏.四妙君逸软膏换药用于促进肛肠病术后创面愈合的研究[J].中国新药杂志,2012,21(3):18.
[8]何永恒,李艳华.四妙君逸软膏对肛肠病术后疼痛模型大鼠血清TNF-α,IL-6,IL-10影响的实验研究[C].2012医学前沿——中华中医药学会肛肠分会第十四次全国肛肠学术交流大会论文精选,2012.
[9]付小兵,王德文.现代创伤修复学[M].北京:人民军医出版社,1999.
[10]Irving BA,Weltman JY,Brock DW,et al.NIH Image J and slice-omatic computed tomography imaging software to quantify soft tissue[J].Obesity,2007,15(2):370-376.
[11]黄康,陈玉林.创面愈合评价指标进展[J].中国修复重建外科杂志,2001,15(2):126-129.
[12]隋志甫,刘静杰,谷延敏,等.创面修复中骨桥蛋白和表皮生长因子受体表达变化的实验研究[J].中国医药导报,2011,8(17):22-24.
[13]Behm B,Babilas P,Landthaler M,et al.Cytokines,chemokines and growth factors in wound healing[J].Journal of the European Academy of Dermatology and Venereology,2012,26(7):812-820.
[14]王大鹏,张桂梅,罗力佳.细胞生长因子修复骨骼肌损伤[J].中国组织工程研究,2016,20(2):273-278.
[15]吴伟.重组人表皮生长因子与碱性成纤维生长因子促进角膜上皮损伤修复及血管新生[J].广州:南方医科大学,2012.
[16]Dinh T,Braunagel S,Rosenblum BI.Growth Factors in Wound Healing:The Present and the Future?[J].Clinics in podiatric medicine and surgery,2015,32(1):109-119.
[17]The molecular and cellular biology of wound repair[M].Berlin:Springer Science&Business Media,2013.
[18]Eldad A,Weinberg A,Breiterman S,et al.Early nonsurgical removal of chemically injured tissue enhances wound healing in partial thickness burns[J].Burns,1998,24(2):166-172.