南麦号系列小麦品种的染色体结构演变
|
摘要
为了明确南麦号系列小麦品种及其亲本的染色体结构特点,用4种寡核苷酸探针和非变性荧光原位杂交(ND-FISH)技术对南麦号小麦品种及其亲本的染色体进行了分析。结果表明,亲本攀早抗含1RS/1BL易位染色体,它的4个衍生品种中3个含1RS/1BL易位染色体。寡核苷酸探针Oligo-275.1、Oligo-275.2、Oligo-pSc119.2-1和Oligo-pTa535-1反映出了2A、4A、5A、6A、7A、3B、5B、6B、1D和2D染色体在攀早抗及其衍生品种间的结构差异。根据Oligo-275.1和Oligo-275.2的信号模式,推测7A染色体在南麦302、特研麦南88和荣春南麦1号中的重组情况不同。
Structural variation of wheat chromosomes can be displayed using fluorescence in situ hybridization(FISH) technology. To investigate the chromosomal structure of Nanmai wheat varieties and their parental lines,four kinds of oligonucleotide probes and non-denaturing fluorescence in situ hybridization(ND-FISH) technology were used to analyze the chromosomes of Nanmai wheat varieties and their parental lines.The results indicated that parental line Panzaokang contains 1 RS/1 BL translocation chromosomes,and three of the four derived varieties also contain 1 RS/1 BL translocation chromosomes. The structural variation of 2 A,4 A,5 A,6 A,7 A,3 B,5 B,6 B,1 D and 2 D chromosomes were displayed by oligonucleotide probes Oligo-275.1,Oligo-275.2,Oligo-pSc119.2-1 and Oligo-pTa535-1. According to the signal patterns of Oligo-275.1 and Oligo-275.2,it is speculated that the recombination patterns of 7 A chromosomes among the three varieties Nanmai 302,Teyanmai Nan 88 and Rongchun Nanmai 1 are different.
Structural variation of wheat chromosomes can be displayed using fluorescence in situ hybridization(FISH) technology. To investigate the chromosomal structure of Nanmai wheat varieties and their parental lines,four kinds of oligonucleotide probes and non-denaturing fluorescence in situ hybridization(ND-FISH) technology were used to analyze the chromosomes of Nanmai wheat varieties and their parental lines.The results indicated that parental line Panzaokang contains 1 RS/1 BL translocation chromosomes,and three of the four derived varieties also contain 1 RS/1 BL translocation chromosomes. The structural variation of 2 A,4 A,5 A,6 A,7 A,3 B,5 B,6 B,1 D and 2 D chromosomes were displayed by oligonucleotide probes Oligo-275.1,Oligo-275.2,Oligo-pSc119.2-1 and Oligo-pTa535-1. According to the signal patterns of Oligo-275.1 and Oligo-275.2,it is speculated that the recombination patterns of 7 A chromosomes among the three varieties Nanmai 302,Teyanmai Nan 88 and Rongchun Nanmai 1 are different.
引文
[1]肖永贵,殷贵鸿,李慧慧,等.小麦骨干亲本周8425B及其衍生品种的遗传解析和抗条锈病基因定位[J].中国农业科学,2011,44(19):3919.XIAO Y G,YIN G H,LI H H,et al.Genetic diversity and genome-wide association analysis of stripe rust resistance among the core wheat parent Zhou 8425B and its derivatives [J].Sientia Agricultura Sinica,2011,44(19):3919.
[2]张德强,宋晓朋,冯洁,等.小麦周8425B及其衍生品种与黄淮麦区主栽品种的遗传解析[J].麦类作物学报,2016,36(10):1328.ZHANG D Q,SONG X P,FENG J,et al.Genetic dissection on the derived lines from wheat cultivar Zhou 8425B and widely grown cultivars in Huang-huai region [J].Journal of Triticeae Crops,2016,36(10):1328.
[3]JIANG P,ZHANG P P,ZHANG X,et al.Genetic contribution of Ningmai 9 wheat to its derivatives evaluated by using SNP markers [J].International Journal of Genomics,2016:3602986.
[4]GUO J,SHI W,ZHANG Z,et al.Association of yield-related traits in founder genotypes and derivatives of common wheat(Triticu maestivumL.)[J].BMC Plant Biology,2018,18:38.
[5]盖红梅,李玉刚,王瑞英,等.鲁麦14对山东新选育小麦品种的遗传贡献[J].作物学报,2012,38(6):954.GE H M,LI Y G,WANG R Y,et al.Genetic contribution of Lumai 14 to novel wheat varieties developed in Shandong Province [J].Acta Agronomica Sinica,2012,38(6):954.
[6]亓佳佳,韩芳,马守才,等.小麦骨干亲本小偃6号及其衍生品种(系)的遗传解析[J].西北农林科技大学学报(自然科学版),2015,43(11):45.QI J J,HAN F,MA S C,et al.Genetic dissection of wheat milestone parent Xiaoyan 6 and its derivatives [J].Journal of Northwest A&F University(Natural Science Edition),2015,43(11):45.
[7]任勇,李生荣,罗建明,等.绵麦37特异位点在其衍生品种中的遗传贡献率分析[J].遗传,2014,36(2):145.REN Y,LI S R,LUO J M,et al.Frenquency and contribution of specific genetic loci transferred from wheat cultivar Mianmai 37 to its derivatives [J].Hereditas,2014,36(2):145.
[8]邓梅,何员江,苟璐璐,等.小麦骨干亲本繁6产量相关性状关键基因组区段的遗传效应[J].作物学报,2018,44(5):706.DENG M,HE Y J,GOU L L,et al.Genetic effects of key genomic regions controlling yield-related traits in wheat founder parent Fan 6 [J].Acta Agronomica Sinica,2018,44(5):706.
[9]CUADRADO á,GOLCZYK H,JOUVE N.A novel,simple and rapid nondenaturing FISH(ND-FISH) technique for the detection of plant telomeres.Potential used and possible target structures detected [J].Chromosome Research,2009,17(6):755.
[10]TANG Z X,YANG Z J,FU S L.Oligonucleotides replacing the roles of repetitive sequences pAs1,pSc119.2,pTa-535,pTa71,CCS1,and pAWRC.1 for FISH analysis [J].Journal of Applied Genetics,2014,55(3):313.
[11]FU S L,CHEN L,WANG Y Y,et al.Oligonucleotide probes for ND-FISH analysis to identify rye and wheat chromosomes [J].Scientific Reports,2015,5:10552.
[12]TANG S Y,QIU L,XIAO Z Q,et al.New oligonucleotide probes for ND-FISH analysis to identify barley chromosomes and to investigate polymorphisms of wheat chromosomes [J].Genes,2016,7:118.
[13]XIAO Z Q,TANG S Y,QIU L,et al.Oligonucleotides and ND-FISH displaying different arrangements of tandem repeats and identification of Dasypyrum villosum chromosomes in wheat backgrounds [J].Molecules,2017,22:973.
[14]JIANG M,XIAO Z Q,FU S L,et al.FISH karyotype of 85 wheat cultivars/lines displayed by ND-FISH using oligonucleotide probes [J].Cereal Research Communications,2017,45(4):549.
[15]HUANG X Y,ZHU M Q,ZHUANG L F,et al.Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH [J].Theoretical and Applied Genetics,2018,131(9):1967.
[16]HAN F P,LAMB J C,BIRCHLER J A.High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize [J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(9):3238.
[17]KOMURO S,ENDO R,SHIKATA K,et al.Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure [J].Genome,2013,56(3):131.
[18]SCHNEIDER A,LINC G,MOLNR-LNG M.Fluorescence in situ hybridization polymorphism using two repetitive DNA clones in different cultivars of wheat [J].Plant Breeding,2003,122(5):396.
[19]CARVALHO A,GUEDES-PINTO H,LIMA-BRITO J.Polymorphism of the simple sequence repeat(AAC)5 in the nucleolar chromosomes of old Portuguese wheat cultivars [J].Journal of Genetics,2013,92(3):583.
[20]杨漫宇,杨足君,杨武云,等.多重易位小麦新品种(系)的选育及细胞学鉴定[J].麦类作物学报,2018,38(2):127.YANG M Y,YANG Z J,YANG W Y,et al.Development and identification of new wheat varieties(lines) with multiple translocation chromosomes via cytogenetic method [J].Journal of Triticeae Crops,2018,38(2):127.
[2]张德强,宋晓朋,冯洁,等.小麦周8425B及其衍生品种与黄淮麦区主栽品种的遗传解析[J].麦类作物学报,2016,36(10):1328.ZHANG D Q,SONG X P,FENG J,et al.Genetic dissection on the derived lines from wheat cultivar Zhou 8425B and widely grown cultivars in Huang-huai region [J].Journal of Triticeae Crops,2016,36(10):1328.
[3]JIANG P,ZHANG P P,ZHANG X,et al.Genetic contribution of Ningmai 9 wheat to its derivatives evaluated by using SNP markers [J].International Journal of Genomics,2016:3602986.
[4]GUO J,SHI W,ZHANG Z,et al.Association of yield-related traits in founder genotypes and derivatives of common wheat(Triticu maestivumL.)[J].BMC Plant Biology,2018,18:38.
[5]盖红梅,李玉刚,王瑞英,等.鲁麦14对山东新选育小麦品种的遗传贡献[J].作物学报,2012,38(6):954.GE H M,LI Y G,WANG R Y,et al.Genetic contribution of Lumai 14 to novel wheat varieties developed in Shandong Province [J].Acta Agronomica Sinica,2012,38(6):954.
[6]亓佳佳,韩芳,马守才,等.小麦骨干亲本小偃6号及其衍生品种(系)的遗传解析[J].西北农林科技大学学报(自然科学版),2015,43(11):45.QI J J,HAN F,MA S C,et al.Genetic dissection of wheat milestone parent Xiaoyan 6 and its derivatives [J].Journal of Northwest A&F University(Natural Science Edition),2015,43(11):45.
[7]任勇,李生荣,罗建明,等.绵麦37特异位点在其衍生品种中的遗传贡献率分析[J].遗传,2014,36(2):145.REN Y,LI S R,LUO J M,et al.Frenquency and contribution of specific genetic loci transferred from wheat cultivar Mianmai 37 to its derivatives [J].Hereditas,2014,36(2):145.
[8]邓梅,何员江,苟璐璐,等.小麦骨干亲本繁6产量相关性状关键基因组区段的遗传效应[J].作物学报,2018,44(5):706.DENG M,HE Y J,GOU L L,et al.Genetic effects of key genomic regions controlling yield-related traits in wheat founder parent Fan 6 [J].Acta Agronomica Sinica,2018,44(5):706.
[9]CUADRADO á,GOLCZYK H,JOUVE N.A novel,simple and rapid nondenaturing FISH(ND-FISH) technique for the detection of plant telomeres.Potential used and possible target structures detected [J].Chromosome Research,2009,17(6):755.
[10]TANG Z X,YANG Z J,FU S L.Oligonucleotides replacing the roles of repetitive sequences pAs1,pSc119.2,pTa-535,pTa71,CCS1,and pAWRC.1 for FISH analysis [J].Journal of Applied Genetics,2014,55(3):313.
[11]FU S L,CHEN L,WANG Y Y,et al.Oligonucleotide probes for ND-FISH analysis to identify rye and wheat chromosomes [J].Scientific Reports,2015,5:10552.
[12]TANG S Y,QIU L,XIAO Z Q,et al.New oligonucleotide probes for ND-FISH analysis to identify barley chromosomes and to investigate polymorphisms of wheat chromosomes [J].Genes,2016,7:118.
[13]XIAO Z Q,TANG S Y,QIU L,et al.Oligonucleotides and ND-FISH displaying different arrangements of tandem repeats and identification of Dasypyrum villosum chromosomes in wheat backgrounds [J].Molecules,2017,22:973.
[14]JIANG M,XIAO Z Q,FU S L,et al.FISH karyotype of 85 wheat cultivars/lines displayed by ND-FISH using oligonucleotide probes [J].Cereal Research Communications,2017,45(4):549.
[15]HUANG X Y,ZHU M Q,ZHUANG L F,et al.Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH [J].Theoretical and Applied Genetics,2018,131(9):1967.
[16]HAN F P,LAMB J C,BIRCHLER J A.High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize [J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(9):3238.
[17]KOMURO S,ENDO R,SHIKATA K,et al.Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure [J].Genome,2013,56(3):131.
[18]SCHNEIDER A,LINC G,MOLNR-LNG M.Fluorescence in situ hybridization polymorphism using two repetitive DNA clones in different cultivars of wheat [J].Plant Breeding,2003,122(5):396.
[19]CARVALHO A,GUEDES-PINTO H,LIMA-BRITO J.Polymorphism of the simple sequence repeat(AAC)5 in the nucleolar chromosomes of old Portuguese wheat cultivars [J].Journal of Genetics,2013,92(3):583.
[20]杨漫宇,杨足君,杨武云,等.多重易位小麦新品种(系)的选育及细胞学鉴定[J].麦类作物学报,2018,38(2):127.YANG M Y,YANG Z J,YANG W Y,et al.Development and identification of new wheat varieties(lines) with multiple translocation chromosomes via cytogenetic method [J].Journal of Triticeae Crops,2018,38(2):127.