硝普钠对生姜根茎抗氧化酶和苯丙烷途径的影响
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摘要
以生姜根茎为试材,研究采后不同浓度硝普钠溶液浸泡处理10 min对其呼吸强度和失重率的影响并筛选最佳浓度,同时分析最佳浓度处理后生姜根茎抗氧化酶活性及苯丙烷途径关键酶活性和产物含量的变化。结果表明,0.25 mmol/L硝普钠处理降低了生姜根茎呼吸强度、抑制了失重率的升高,硝普钠处理还显著提高了生姜根茎过氧化氢含量、超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶的活性。此外,硝普钠处理还提高了苯丙氨酸解氨酶活性、显著提高了4-香豆酰CoA连接酶的活性,促进了木质素、总酚和类黄酮的积累。由此表明,硝普钠处理通过调节生姜根茎抗氧化酶活性和苯丙烷途径延缓其衰老。
Ginger rhizomes were used as materials to study the effect of sodium nitroprusside( SNP) dipping at different concentration for 10 min on the respiratory intensity and weight loss to screen the optimal concentration of SNP.The changes of antioxidant enzymes activities,key enzymes in phenylpropanoid pathway and metabolites accumulation in ginger rhizomes after SNP treatment were also studied.The results indicated that 0.25 mmol/L SNP treatment decreased the respiratory intensity and inhibited the increase of weight loss. SNP treatment significantly increased the content of hydrogen peroxide( H_2O_2) and the activity of superoxide dismutase( SOD),peroxidase( POD) and ascorbate peroxidase( APX) in ginger rhizomes.The results also showed that SNP treatment enhanced the activity of phenylalanine ammonia-lyase( PAL) and significantly increased 4-coumarate/coenzyme A ligase( 4CL) activity to activate the accumulation of lignin,total phenolic compounds and flavonoids in ginger rhizomes.These results suggested that SNP delay senescence of ginger rhizomes by modulating antioxidant enzymes and phenylpropanoid pathway.
Ginger rhizomes were used as materials to study the effect of sodium nitroprusside( SNP) dipping at different concentration for 10 min on the respiratory intensity and weight loss to screen the optimal concentration of SNP.The changes of antioxidant enzymes activities,key enzymes in phenylpropanoid pathway and metabolites accumulation in ginger rhizomes after SNP treatment were also studied.The results indicated that 0.25 mmol/L SNP treatment decreased the respiratory intensity and inhibited the increase of weight loss. SNP treatment significantly increased the content of hydrogen peroxide( H_2O_2) and the activity of superoxide dismutase( SOD),peroxidase( POD) and ascorbate peroxidase( APX) in ginger rhizomes.The results also showed that SNP treatment enhanced the activity of phenylalanine ammonia-lyase( PAL) and significantly increased 4-coumarate/coenzyme A ligase( 4CL) activity to activate the accumulation of lignin,total phenolic compounds and flavonoids in ginger rhizomes.These results suggested that SNP delay senescence of ginger rhizomes by modulating antioxidant enzymes and phenylpropanoid pathway.
引文
[1]朱丹实,刘仁斌,杜伟,等.生姜成分差异及采后贮藏保鲜技术研究进展[J].食品工业科技,2015,36(17):375-378,383.
[2]赵文竹,张瑞雪,于志鹏,等.生姜的化学成分及生物活性图7采后硝普钠处理对生姜根茎总酚(A),类黄酮(B)和木质素(C)含量的影响Fig.7 Effect of sodium nitrate treatment on the total phenololic compounds(A),flavonoids(B)and lignin(C)content in ginger rhizomes研究进展[J].食品工业科技,2016,37(11):383-389.
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[5]Barman K,Siddiqui M W,Patel V B,et al. Nitric oxide reduces pericarp browning and preserves bioactive antioxidants in litchi[J].Scientia Horticulturae,2014,171(171):71-77.
[6]Wu F H,Yang H Q,Chang Y Z,et al.Effects of nitric oxide on reactive oxygen species and antioxidant capacity in Chinese bayberry during storage[J]. Scientia Horticulturae,2012,135(135):106-111.
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[8]Dong J F,Zhang M,Lu L,et al. Nitric oxide fumigation stimulates flavonoid and phenolic accumulation and enhances antioxidant activity of mushroom[J]. Food Chemistry,2012,135(3):1220-1225.
[9]Hu M J,Yang D P,Huber D J,et al.Reduction of postharvestanthracnose and enhancement of disease resistance in ripening mango fruit by nitric oxide treatment[J]. Postharvest Biology and Technology,2014,97(11):115-122.
[10]黄玉平,彭文娟,张瑜,等.NO处理对草莓果实采后品质和苯丙烷类代谢的影响[J].核农学报,2016,30(10):1959-1966.
[11]敬媛媛,吴斌,李艳娇,等.一氧化氮处理对甜瓜果实采后病害的控制及活性氧代谢的作用[J].现代食品科技,2016(12):186-190.
[12]李秀,巩彪,徐坤.外源NO对高温胁迫下姜叶片活性氧代谢的影响[J].园艺学报,2014,41(2):277-284.
[13]Bowyer M C,Wills R B H,Badiyan D,et al.Extending the postharvest life of carnations with nitric oxide-comparison of fumigation and in vivo delivery[J]. Postharvest Biology and Technology,2003,30(3):281-286.
[14]Shi J Y,Gao L P,Zuo J H,et al. Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life,enhances antioxidant enzyme activity,and inhibits chlorophylldegradation[J]. Postharvest Biology and Technology,2016,116:98-104.
[15]Saba M K,Moradi S. Sodium nitroprusside(SNP)spray to maintain fruit quality and alleviate postharvest chilling injury of peach fruit[J].Scientia Horticulturae,2017,216:193-199.
[16]张琦,刘千,张智,等.硝普钠对香蕉采后品质及生理指标的影响[J].南方农业学报,2016,47(7):1198-1202.
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[18]Wang Y S,Tian S P,Xu Y. Effects of high oxygen concentration on pro-and anti-oxidant enzymes in peach fruits during postharvest periods[J]. Food Chemistry,2005,91(1):99-104.
[19]Chen C,Hu W Z,He Y B,et al. Effect of citric acid combined with UV-C on the quality of fresh-cut apples[J].Postharvest Biology and Technology,2016,111:126-131.
[20]Rahnama H,Ebrahimzadeh H. The effect of Na Cl on antioxidant enzyme activities in potato seedlings[J]. Biologia Plantarum,2005,49(1):93-97.
[21]闫媛媛,胡文忠,姜爱丽,等.茉莉酸甲酯和乙烯利处理对鲜切苹果膜质过氧化反应的影响[J].食品工业科技,2015,36(14):345-349.
[22]王焕宇,姜璐璐,王惠源,等.短波紫外线对草莓采后腐烂、苯丙烷类代谢和抗氧化活性的影响[J].食品科学,2015,36(10):221-226.
[23]胡文忠,姜爱丽,杨宏,等.茉莉酸甲酯对鲜切苹果生理生化变化的影响[J].食品工业科技,2012,33(16):338-341,346.
[24]Arensberg P,Hemmingsen V H,Nyholm N.A miniscale algal toxicity test[J].Chemosphere,1995,30(11):2103-2115.
[25]葛永红,李灿婴,王毅,等.硅酸钠处理对杏果实活性氧和苯丙烷代谢的影响[J].食品工业科技,2014,35(13):317-320,326.
[26]Ge Y H,Deng H W,Bi Y,et al.Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon(Cucumis melo L.)fruit[J].Postharvest Biology and Technology,2015,99:160-167.
[27]李凯龙,王艺潼,韩晓雪,等.低钾胁迫对番茄叶片活性氧及抗氧化酶系的影响[J].西北植物学报,2013,33(1):66-73.
[28]Shabanian S,Esfahani M N,Karamian R,et al.Physiological and biochemical modifications by postharvest treatment with sodium nitroprusside extend vase life of cut flowers of two gerbera cultivars[J].Postharvest Biology Technology,2018,137:1-8.
[29]周拥军,郜海燕,陈杭君,等.减压贮藏对杏鲍菇采后活性氧代谢的影响[J].核农学报,2015,29(6):1108-1113.
[30]范林林,王清,左进华,等.外源NO处理对茄子贮藏品质的影响[J].中国食品学报,2017,17(1):186-192.
[31]胡江伟,周江,朱璇,等.一氧化氮对新疆伽师瓜果实采后贮藏品质的影响[J].食品工业科技,2015,36(13):352-356.
[32]Kuzzaniak E. Effects of fusaric acid on reactive oxygen species and antioxidants in tomato cell cultures[J]. Journal of Phytopathology,2001,149(10):575-582.
[33]黄玉平,彭文娟,张瑜,等.NO处理对草莓果实采后品质和苯丙烷类代谢的影响[J].核农学报,2016,30(10):1959-1966.
[34]倪志勇,王娟,吕萌,等.棉花4-香豆酸辅酶A连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.
[35]葛永红,李灿婴,朱丹实,等.采后BTH处理对苹果果实苯丙烷代谢和病程相关蛋白积累的增强作用[J].食品工业科技,2015,36(5):306-310.
[36]朱迎迎.外源一氧化氮(NO)诱导火龙果果实对炭疽病的抗性及其对采后品质影响的研究[D].海口:海南大学,2016.
[2]赵文竹,张瑞雪,于志鹏,等.生姜的化学成分及生物活性图7采后硝普钠处理对生姜根茎总酚(A),类黄酮(B)和木质素(C)含量的影响Fig.7 Effect of sodium nitrate treatment on the total phenololic compounds(A),flavonoids(B)and lignin(C)content in ginger rhizomes研究进展[J].食品工业科技,2016,37(11):383-389.
[3]向艳昌.浅论生姜的贮藏保鲜技术[J].农业开发与装备,2017,(12):141.
[4]Liu L Q,Dong Y,Guan J F. Effects of nitric oxide on the quality and pectin metabolism of Yali Pears during cold storage[J].Agricultural Sciences in China,2011,10(7):1125-1133.
[5]Barman K,Siddiqui M W,Patel V B,et al. Nitric oxide reduces pericarp browning and preserves bioactive antioxidants in litchi[J].Scientia Horticulturae,2014,171(171):71-77.
[6]Wu F H,Yang H Q,Chang Y Z,et al.Effects of nitric oxide on reactive oxygen species and antioxidant capacity in Chinese bayberry during storage[J]. Scientia Horticulturae,2012,135(135):106-111.
[7]Ferrer J L,Austin M B,Stewart C,et al.Structure and function of enzymes involved in the biosynthesis of phenylpropanoids[J].Plant Physiology and Biochemistry,2007,46(3):356-370.
[8]Dong J F,Zhang M,Lu L,et al. Nitric oxide fumigation stimulates flavonoid and phenolic accumulation and enhances antioxidant activity of mushroom[J]. Food Chemistry,2012,135(3):1220-1225.
[9]Hu M J,Yang D P,Huber D J,et al.Reduction of postharvestanthracnose and enhancement of disease resistance in ripening mango fruit by nitric oxide treatment[J]. Postharvest Biology and Technology,2014,97(11):115-122.
[10]黄玉平,彭文娟,张瑜,等.NO处理对草莓果实采后品质和苯丙烷类代谢的影响[J].核农学报,2016,30(10):1959-1966.
[11]敬媛媛,吴斌,李艳娇,等.一氧化氮处理对甜瓜果实采后病害的控制及活性氧代谢的作用[J].现代食品科技,2016(12):186-190.
[12]李秀,巩彪,徐坤.外源NO对高温胁迫下姜叶片活性氧代谢的影响[J].园艺学报,2014,41(2):277-284.
[13]Bowyer M C,Wills R B H,Badiyan D,et al.Extending the postharvest life of carnations with nitric oxide-comparison of fumigation and in vivo delivery[J]. Postharvest Biology and Technology,2003,30(3):281-286.
[14]Shi J Y,Gao L P,Zuo J H,et al. Exogenous sodium nitroprusside treatment of broccoli florets extends shelf life,enhances antioxidant enzyme activity,and inhibits chlorophylldegradation[J]. Postharvest Biology and Technology,2016,116:98-104.
[15]Saba M K,Moradi S. Sodium nitroprusside(SNP)spray to maintain fruit quality and alleviate postharvest chilling injury of peach fruit[J].Scientia Horticulturae,2017,216:193-199.
[16]张琦,刘千,张智,等.硝普钠对香蕉采后品质及生理指标的影响[J].南方农业学报,2016,47(7):1198-1202.
[17]曹建康,姜微波,赵玉梅,等.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.
[18]Wang Y S,Tian S P,Xu Y. Effects of high oxygen concentration on pro-and anti-oxidant enzymes in peach fruits during postharvest periods[J]. Food Chemistry,2005,91(1):99-104.
[19]Chen C,Hu W Z,He Y B,et al. Effect of citric acid combined with UV-C on the quality of fresh-cut apples[J].Postharvest Biology and Technology,2016,111:126-131.
[20]Rahnama H,Ebrahimzadeh H. The effect of Na Cl on antioxidant enzyme activities in potato seedlings[J]. Biologia Plantarum,2005,49(1):93-97.
[21]闫媛媛,胡文忠,姜爱丽,等.茉莉酸甲酯和乙烯利处理对鲜切苹果膜质过氧化反应的影响[J].食品工业科技,2015,36(14):345-349.
[22]王焕宇,姜璐璐,王惠源,等.短波紫外线对草莓采后腐烂、苯丙烷类代谢和抗氧化活性的影响[J].食品科学,2015,36(10):221-226.
[23]胡文忠,姜爱丽,杨宏,等.茉莉酸甲酯对鲜切苹果生理生化变化的影响[J].食品工业科技,2012,33(16):338-341,346.
[24]Arensberg P,Hemmingsen V H,Nyholm N.A miniscale algal toxicity test[J].Chemosphere,1995,30(11):2103-2115.
[25]葛永红,李灿婴,王毅,等.硅酸钠处理对杏果实活性氧和苯丙烷代谢的影响[J].食品工业科技,2014,35(13):317-320,326.
[26]Ge Y H,Deng H W,Bi Y,et al.Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon(Cucumis melo L.)fruit[J].Postharvest Biology and Technology,2015,99:160-167.
[27]李凯龙,王艺潼,韩晓雪,等.低钾胁迫对番茄叶片活性氧及抗氧化酶系的影响[J].西北植物学报,2013,33(1):66-73.
[28]Shabanian S,Esfahani M N,Karamian R,et al.Physiological and biochemical modifications by postharvest treatment with sodium nitroprusside extend vase life of cut flowers of two gerbera cultivars[J].Postharvest Biology Technology,2018,137:1-8.
[29]周拥军,郜海燕,陈杭君,等.减压贮藏对杏鲍菇采后活性氧代谢的影响[J].核农学报,2015,29(6):1108-1113.
[30]范林林,王清,左进华,等.外源NO处理对茄子贮藏品质的影响[J].中国食品学报,2017,17(1):186-192.
[31]胡江伟,周江,朱璇,等.一氧化氮对新疆伽师瓜果实采后贮藏品质的影响[J].食品工业科技,2015,36(13):352-356.
[32]Kuzzaniak E. Effects of fusaric acid on reactive oxygen species and antioxidants in tomato cell cultures[J]. Journal of Phytopathology,2001,149(10):575-582.
[33]黄玉平,彭文娟,张瑜,等.NO处理对草莓果实采后品质和苯丙烷类代谢的影响[J].核农学报,2016,30(10):1959-1966.
[34]倪志勇,王娟,吕萌,等.棉花4-香豆酸辅酶A连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.
[35]葛永红,李灿婴,朱丹实,等.采后BTH处理对苹果果实苯丙烷代谢和病程相关蛋白积累的增强作用[J].食品工业科技,2015,36(5):306-310.
[36]朱迎迎.外源一氧化氮(NO)诱导火龙果果实对炭疽病的抗性及其对采后品质影响的研究[D].海口:海南大学,2016.