基于iTRAQ技术研究采后1-甲基环丙烯和乙烯利处理对茭白线粒体蛋白质组变化的影响
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摘要
为探讨茭白采后衰老的分子机制,应用同位素标记相对与绝对定量蛋白质组学技术研究了茭白常温贮藏期间线粒体蛋白质表达谱的变化及1-甲基环丙烯(1-methyleyelopropene,1-MCP)和乙烯利(ethylene,ET)处理对茭白线粒体蛋白质组变化的影响。结果表明:共鉴定到肽段数大于等于2的可信蛋白1 908个,与贮藏0 d相比,对照(CK)组、ET和1-MCP处理组茭白贮藏3 d和6 d后,共有315个蛋白表达量变化倍数在2.0倍以上且重复组数据统计学差异显著(P<0.05)。生物信息学分析显示代谢途径、次生代谢产物生物合成、氨基酸生物合成与代谢、核苷酸代谢、含碱基小分子代谢途径等可能与茭白采后衰老有关,三羧酸循环、氧化磷酸化、磷酸戊糖途径、C5支链二元酸代谢及氨基酸代谢途径可能在茭白采后衰老中发挥重要作用。这些差异表达蛋白的生物学功能分析表明,茭白采后碳水化合物水解加速,磷酸戊糖途径加强而糖酵解途径和氧化磷酸化减弱,导致能量合成减少,同时形成氧化胁迫,这可能激活Ca~(2+)/MAPKs、细胞色素c和茉莉酸等信号途径,造成初级代谢紊乱和次级代谢产物(如木质素)积累,从而促进细胞凋亡或细胞坏死,最终加速衰老。
In order to explore the molecular mechanism of postharvest senescence of Zizania latifolia, the effect of 1-methyleyelopropene(1-MCP) and ethylene(ET) treatments on mitochondrial proteome changes in postharvestZ. latifolia after storage at 25 ℃ for 0(control), 3 and 6 days were investigated by using isobaric tags for relative and absolute quantification(iTRAQ) labeling. The results showed that a total of 1 908 proteins were identified with at least two peptides, of which 315 proteins showed an 2.0-fold change in their relative quantitation in the untreated, ET-treated, 1-MCPtreated groups at day 3 and 6 of storage as compared to their counterparts at day 0, with statistically significant differences being observed between results from replicate experiments(P < 0.05). A bioinformatics analysis of these differentially expressed proteins revealed that metabolic pathways, the biosynthesis of secondary metabolites, the biosynthesis and metabolism of amino acids, nucleotide metabolism, and the metabolic pathways of nucleobase-containing small molecules were related to postharvest senescence of Z. latifolia and that the citrate cycle, oxidative phosphorylation(OXPHOS), the pentose phosphate pathway(PPP), C5-branched dibasic acid metabolism and amino acid metabolism may play critical roles in postharvest senescence of Z. latifolia. Based on their biological functions, postharvest senescence of Z. latifolia may be closely associated with accelerated carbohydrate hydrolysis as well as strengthened PPP and weakened glycolysis and OXPHOS, leading to reduced energy supply and aggravated oxidative damage and consequently activating Ca~(2+)/MAPKs, cytochrome c and jasmonate signaling pathways to cause primary metabolic disturbance and an increase in the accumulation of secondary metabolites such as lignin and consequently induce cell apoptosis or necrosis and finally accelerate senescence.
In order to explore the molecular mechanism of postharvest senescence of Zizania latifolia, the effect of 1-methyleyelopropene(1-MCP) and ethylene(ET) treatments on mitochondrial proteome changes in postharvestZ. latifolia after storage at 25 ℃ for 0(control), 3 and 6 days were investigated by using isobaric tags for relative and absolute quantification(iTRAQ) labeling. The results showed that a total of 1 908 proteins were identified with at least two peptides, of which 315 proteins showed an 2.0-fold change in their relative quantitation in the untreated, ET-treated, 1-MCPtreated groups at day 3 and 6 of storage as compared to their counterparts at day 0, with statistically significant differences being observed between results from replicate experiments(P < 0.05). A bioinformatics analysis of these differentially expressed proteins revealed that metabolic pathways, the biosynthesis of secondary metabolites, the biosynthesis and metabolism of amino acids, nucleotide metabolism, and the metabolic pathways of nucleobase-containing small molecules were related to postharvest senescence of Z. latifolia and that the citrate cycle, oxidative phosphorylation(OXPHOS), the pentose phosphate pathway(PPP), C5-branched dibasic acid metabolism and amino acid metabolism may play critical roles in postharvest senescence of Z. latifolia. Based on their biological functions, postharvest senescence of Z. latifolia may be closely associated with accelerated carbohydrate hydrolysis as well as strengthened PPP and weakened glycolysis and OXPHOS, leading to reduced energy supply and aggravated oxidative damage and consequently activating Ca~(2+)/MAPKs, cytochrome c and jasmonate signaling pathways to cause primary metabolic disturbance and an increase in the accumulation of secondary metabolites such as lignin and consequently induce cell apoptosis or necrosis and finally accelerate senescence.
引文
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[3]CAO S F,YANG Z F,ZHENG Y H,et al.Effect of 1-methylcyclopene on senescence and quality maintenance of green bell pepper fruit during storage at 20℃[J].Postharvest Biology&Technology,2012,70:1-6.DOI:10.1016/j.postharvbio.2012.03.005.
[4]XIE Q L,HU Z L,ZHU Z G,et al.Overexpression of a novel MADS-box gene SlFYFL delays senescence,fruit ripening and abscission in tomato[J].Scientific Reports,2014,4(1):1-10.DOI:10.1038/srep04367.
[5]WANG C,CHU J J,FU L L,et al.iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage[J].Food Chemistry,2018,247:73-80.DOI:10.1016/j.foodchem.2017.12.004.
[6]MA J,SHENG H C,LI X L,et al.iTRAQ-based proteomic analysis reveals the mechanisms of silicon-mediated cadmium tolerance in rice(Oryza sativa)cells[J].Plant Physiology and Biochemistry,2016,104:71-80.DOI:10.1016/j.plaphy.2016.03.024.
[7]袁建丰,李林林,孙敏华,等.iTRAQ标记技术及其在微生物比较蛋白质组学中的研究进展[J].中国预防兽医学报,2013,35(10):859-862.DOI:10.3969/j.issn.1008-0589.2013.10.21.
[8]WANG Y Q,ZHANG L J,ZHU S J.1-Methylcyclopropene(1-MCP)-induced protein expression associated with changes in Tsai Tai(Brassica chinensis)leaves during low temperature storage[J].Postharvest Biology&Technology,2014,87:120-125.DOI:10.1016/j.postharvbio.2013.08.016.
[9]杜传来,罗海波,彭昕,等.茭白线粒体蛋白双向电泳体系建立[J].南方农业学报,2016,47(3):332-336.DOI:10.3969/j:issn.2095-1191.2016.03.332.
[10]AGARWAL G,CHOUDHARY D,SINGH V P,et al.Role of ethylene receptors during senescence and ripening in horticultural crops[J].Plant Signaling&Behavior,2012,7(7):827-846.DOI:10.4161/psb.20321.
[11]TAKAHA T,YANASE M,OKADA S,et al.Disproportionating enzyme(4-alpha-glucanotransferase;EC 2.4.1.25)of potato:purification,molecular cloning,and potential role in starch metabolism[J].Journal of Biological Chemistry,1993,268(2):1391-1396.
[12]STEUP M,SCH?CHTELE C.α-1,4-glucan phosphorylase forms from leaves of spinach(Spinacia oleracea L.):Ⅱ.peptide patterns and immunological properties.a comparison with other phosphorylase forms[J].Planta,1986,168(2):222-231.DOI:10.1007/BF00391219.
[13]SCHM?LZER K,GUTMANN A,DIRICKS M,et al.Sucrose synthase:a unique glycosyltransferase for biocatalytic glycosylation process development[J].Biotechnology Advances,2016,34(2):88-111.DOI:10.1016/j.biotechadv.2015.11.003.
[14]ROJAS M,TIESSEN A,ASCENCIO F,et al.Two Promoters of beta-glucosidase paralogs(ZmBGlu2 and ZmBGlu5)highly active in tropical young maize hybrid seedlings[J].Plant Molecular Biology Reporter,2015,33(6):1666-1674.DOI:10.1007/s11105-015-0863-0.
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[23]BURGESS D J.Chromatin:histone influences on chromosomal translocations[J].Nature Reviews Molecular Cell Biology,2015,16(8):452-453.DOI:10.1038/nrm4028.
[24]DEMIDCHIK V,STRALTSOVA D,MEDVEDEV S S,et al.Stressinduced electrolyte leakage:the role of K+-permeable channels and involvement in programmed cell death and metabolic adjustment[J].Journal of Experimental Botany,2014,65(5):1259-1270.DOI:10.1093/jxb/eru004.
[25]LI C Y,LUO C,ZHOU Z H,et al.Gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation[J].BMC Plant Biology,2017,17(1):57.DOI:10.1186/s12870-017-1005-7.
[26]LUO H B,JIANG J,ZHANG L,et al.Effect of gibberellic acid and6-benzylaminopurine on lignification of fresh-cut Zizania latifolia during refrigerated(1℃)storage[J].Journal of Food Processing and Preservation,2013,37(5):864-869.DOI:10.1111/j.1745-4549.2012.00743.x.
[27]ZHANG H,ZHOU C.Signal transduction in leaf senescence[J].Plant Molecular Biology,2013,82(6):539-545.DOI:10.1007/s11103-012-9980-4.
[28]施小龙,邢更妹,汪丽虹,等.植物钙信号系统与体细胞胚发生[J].生命科学,2002,14(5):302-304;271.DOI:10.3969/j.issn.1004-0374.2002.05.013.
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[30]SUBPIPATTANA P,GRAMS R,VICHASRI-GRAMS S.Analysis of a calcium-binding EF-hand protein family in Fasciola gigantica[J].Experimental Parasitology,2012,130(4):364-373.DOI:10.1016/j.exppara.2012.02.005.
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[33]瓮巧云,宋晋辉,张爱香.谷子丝/苏氨酸蛋白激酶类抗病基因同源序列的克隆与分析[J].河南农业科学,2012,41(6):106-108.DOI:10.3969/j.issn.1004-3268.2012.06.024.
[34]MENDES B M J,CARDOSO S C,BOSCARIOL-CAMARGO R L,et al.Reduction in susceptibility to Xanthomonas axonopodis pv.citri in transgenic Citrus sinensis expressing the rice Xa21 gene[J].Plant Pathology,2010,59(1):68-75.DOI:10.1111/j.1365-3059.2009.02148.x.
[35]MARTIN G B,BROMMONSCHENKEL S H,CHUNWONGSE J,et al.Map-based cloning of a protein kinase gene conferring disease resistance in tomato[J].Science,1993,262:1432-1436.DOI:10.1126/science.7902614.
[36]HUROWITZ E H,MELNYK J M,CHEN Y J,et al.Genomic characterization of the human heterotrimeric G protein alpha,beta,and gamma subunit genes[J].DNA Research,2000,7(2):111-120.DOI:10.1093/dnares/7.2.111.
[37]张之为,赵君,樊明寿,等.植物小G蛋白的研究进展[J].西北植物学报,2009,29(3):622-628.DOI:10.3321/j.issn:1000-4025.2009.03.031.
[38]TAKAI Y,SASAKI T,MATOZAKI T.Small GTP-binding proteins[J].Physiology Reviews,2001,81(1):153-208.DOI:10.1152/physrev.2001.81.1.153.
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